DK0702090T3 - Coamplifikation af målnucleinsyrer ved anvendelse af volumenudelukkelsesmiddel i reaktionssammensætning, testkit og testanordning, som er anvendelige dertil - Google Patents

Coamplifikation af målnucleinsyrer ved anvendelse af volumenudelukkelsesmiddel i reaktionssammensætning, testkit og testanordning, som er anvendelige dertil

Info

Publication number
DK0702090T3
DK0702090T3 DK95306480T DK95306480T DK0702090T3 DK 0702090 T3 DK0702090 T3 DK 0702090T3 DK 95306480 T DK95306480 T DK 95306480T DK 95306480 T DK95306480 T DK 95306480T DK 0702090 T3 DK0702090 T3 DK 0702090T3
Authority
DK
Denmark
Prior art keywords
target nucleic
nucleic acids
volume exclusion
amplification
exclusion agent
Prior art date
Application number
DK95306480T
Other languages
Danish (da)
English (en)
Inventor
John Wesley Backus
Original Assignee
Ortho Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc
Application granted granted Critical
Publication of DK0702090T3 publication Critical patent/DK0702090T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK95306480T 1994-09-15 1995-09-14 Coamplifikation af målnucleinsyrer ved anvendelse af volumenudelukkelsesmiddel i reaktionssammensætning, testkit og testanordning, som er anvendelige dertil DK0702090T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/306,792 US5705366A (en) 1994-09-15 1994-09-15 Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor

Publications (1)

Publication Number Publication Date
DK0702090T3 true DK0702090T3 (da) 2004-09-06

Family

ID=23186863

Family Applications (1)

Application Number Title Priority Date Filing Date
DK95306480T DK0702090T3 (da) 1994-09-15 1995-09-14 Coamplifikation af målnucleinsyrer ved anvendelse af volumenudelukkelsesmiddel i reaktionssammensætning, testkit og testanordning, som er anvendelige dertil

Country Status (9)

Country Link
US (1) US5705366A (pt)
EP (1) EP0702090B1 (pt)
JP (1) JP3802110B2 (pt)
AT (1) ATE266102T1 (pt)
CA (1) CA2158486C (pt)
DE (1) DE69532984T2 (pt)
DK (1) DK0702090T3 (pt)
ES (1) ES2219655T3 (pt)
PT (1) PT702090E (pt)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9819418D0 (en) * 1998-09-07 1998-10-28 Secr Defence Amplification method
US6200757B1 (en) 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
JP2001029078A (ja) * 1999-07-16 2001-02-06 Shimadzu Corp Rna増幅法
GB0114639D0 (en) * 2001-06-15 2001-08-08 Sec Dep Of The Home Department Improvements in and relating to analysis of DNA
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
JP4340543B2 (ja) 2002-02-21 2009-10-07 エーエスエム サイエンティフィック, インコーポレイテッド リコンビナーゼポリメラーゼ増幅
EP1411133B1 (en) * 2002-09-24 2009-01-07 Qiagen GmbH Enhanced coamplification of nucleic acids
EP1403379A1 (en) * 2002-09-24 2004-03-31 QIAGEN GmbH Enhanced coamplification of nucleic acids
JP4896006B2 (ja) 2004-04-08 2012-03-14 バイオマトリカ, インコーポレイテッド ライフサイエンスのためのサンプル保存とサンプル管理との統合
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
CA2502549C (en) * 2004-04-23 2016-02-16 Becton, Dickinson And Company Use of an extraction control in a method of extracting nucleic acids
ES2718482T3 (es) * 2004-06-01 2019-07-02 Alere San Diego Inc Kit para amplificación de recombinasa polimerasa
JP2006320217A (ja) * 2005-05-17 2006-11-30 Olympus Corp マルチプレックスpcr法
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック, インコーポレイテッド レコンビナーゼポリメラーゼ増幅
EP2077337A1 (en) * 2007-12-26 2009-07-08 Eppendorf Array Technologies SA Amplification and detection composition, method and kit
EP2432795B1 (en) * 2009-05-20 2015-03-04 Alere San Diego, Inc. Dna glycosylase/lyase and ap endonuclease substrates
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
CN103119178B (zh) * 2010-09-22 2017-05-17 株式会社钟化 核酸的检测方法以及装置、试剂盒
EP2694666B1 (en) 2011-04-07 2018-05-23 Alere San Diego, Inc. Monitoring recombinase polymerase amplification mixtures
EP2743353A1 (en) * 2012-12-13 2014-06-18 Alacris Theranostics GmbH Interstitial amplification
WO2014100755A2 (en) 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
US9587268B2 (en) * 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
EP3387411B1 (en) 2015-12-08 2023-05-10 Biomatrica, INC. Reduction of erythrocyte sedimentation rate
US10731209B2 (en) 2016-12-19 2020-08-04 Quantum-Si Incorporated Loading molecules into sample wells for analysis
AU2018261007A1 (en) 2017-05-05 2019-11-07 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
CN112534064A (zh) * 2018-06-07 2021-03-19 拜奥法尔诊断有限责任公司 快速热循环方法
BR112022006682A2 (pt) 2019-10-11 2022-07-12 Quantum Si Inc Modificação de superfície na fase de vapor

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3798320A (en) * 1970-09-18 1974-03-19 South African Inventions Precipitation of bacterial cells with polymers
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
CA1180647A (en) 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4766064A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
US4813326A (en) 1984-07-16 1989-03-21 Yamaha Corporation Method and apparatus for synthesizing music tones with high harmonic content
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4795698A (en) * 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
US4997771A (en) 1987-04-27 1991-03-05 Schering Corporation Method for measuring the BZ-1 receptor binding activity in a test sample or test compound
US5024935A (en) 1987-12-18 1991-06-18 Eastman Kodak Company Dye-providing composition, diagnostic test kit and their use in method for ligand determination using peroxidase labeled-receptor
US4914210A (en) 1987-10-02 1990-04-03 Cetus Corporation Oligonucleotide functionalizing reagents
US4962029A (en) 1987-10-02 1990-10-09 Cetus Corporation Covalent oligonucleotide-horseradish peroxidase conjugate
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
IL88923A (en) 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5229297A (en) * 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
WO1990008840A1 (en) 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
US4948561A (en) 1989-02-09 1990-08-14 Eastman Kodak Company Multiple level filter device and kit containing same
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5089233A (en) 1989-06-12 1992-02-18 Eastman Kodak Company Processing apparatus for a chemical reaction pack
US5106730A (en) * 1989-07-24 1992-04-21 Microprobe Corporation Lactam-containing compositions and methods useful for the hybridization of nucleic acids
US5231015A (en) 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
CA2031659A1 (en) 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
JP2969222B2 (ja) 1990-06-07 1999-11-02 清水建設株式会社 原子炉の生体遮蔽コンクリート壁の解体装置および原子炉の生体遮蔽コンクリート壁の解体工法
US5155166A (en) 1990-06-18 1992-10-13 Eastman Kodak Company Use of 1-(1-pyrrolidinylcarbonyl)pyridinium salts to attach compounds to carboxylated particles and a kit containing same
US5147777A (en) 1990-06-18 1992-09-15 Eastman Kodak Company Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use
US5173260A (en) 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
EP0487218B1 (en) 1990-10-31 1997-12-29 Tosoh Corporation Method for detecting or quantifying target nucleic acid
ZA918965B (en) * 1990-11-13 1992-08-26 Siska Diagnostics Inc Nucleic acid amplification by two-enzyme,self-sustained sequence replication
JPH0830704B2 (ja) 1991-03-21 1996-03-27 イーストマン コダック カンパニー 核酸増幅のための要素および方法ならびに付着プローブを使用する検出方法
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0543484B1 (en) * 1991-08-30 2001-01-31 Research Development Corporation of Japan A method of DNA amplification
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
SG68568A1 (en) * 1993-07-08 1999-11-16 Johnson & Johnson Clin Diag Method for coamplification of two different nucleic acid sequences using polymerase chain reaction

Also Published As

Publication number Publication date
JP3802110B2 (ja) 2006-07-26
EP0702090A3 (en) 1998-03-04
EP0702090B1 (en) 2004-05-06
ES2219655T3 (es) 2004-12-01
CA2158486A1 (en) 1996-03-16
DE69532984T2 (de) 2005-05-04
CA2158486C (en) 2007-11-06
US5705366A (en) 1998-01-06
DE69532984D1 (de) 2004-06-09
EP0702090A2 (en) 1996-03-20
JPH08103300A (ja) 1996-04-23
ATE266102T1 (de) 2004-05-15
PT702090E (pt) 2004-09-30

Similar Documents

Publication Publication Date Title
DK0702090T3 (da) Coamplifikation af målnucleinsyrer ved anvendelse af volumenudelukkelsesmiddel i reaktionssammensætning, testkit og testanordning, som er anvendelige dertil
DK0726324T3 (da) Anvendelse af exonuklease og/eller glycosylase som supplementer til antipolymeraseantistof til forøgelse af specification i polymerasekædereaktion
EA200100402A1 (ru) Способы амплификации и секвенирования нуклеиновых кислот
DK1705253T3 (da) Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR)
KR970701791A (ko) 등온 가닥 변위 핵산 증폭법(Isothermal Strand Displacement Nucleic Acid Amplification)
ATE244759T1 (de) Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation
BR0101493B1 (pt) mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes.
ATE394508T1 (de) Late-pcr
DE60034396D1 (de) Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure
ATE428809T1 (de) Nukleinsäure-primer und -sonden zur detektion von hiv-1 und hiv-2
DE60022541D1 (de) Polynukleotid amplifizierungsverfahren
ATE220726T1 (de) Oligonukleotid-primer für amplifizierung von hcv- nukleinsäure
DK0632134T3 (da) Reagenser og fremgangsmåder til koblet revers transkription ved høj temperatur og polymerase-kædereaktioner
FI921945A (fi) Foerfaranden foer amplifiering och detektering av nukleinsyror genom attanvaenda snabb polymeraskedjereaktionscykel
DK0648845T3 (da) Metode til co-amplificering af to forskellige nucleinsyresekvenser ved hjælp af polymerasekædereaktion
DE69931928D1 (de) Reversibel inhibierende sonden
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
DK1036847T3 (da) Oligonukleotidprimere til effektiv revers transskription af Hepatitis C Virus (HCV)-RNA og fremgangsmåde til anvendelse deraf
ATE456669T1 (de) Verfahren zur quantifizierung von nukleinsäuren
DE60136572D1 (de) Detektion von rpob sequenzen von mycobacterium tuberculosis
DE60209711D1 (de) Amplifikation und nachweis von mycoplasma pneumoniae