DE69935314D1 - Quantitatitative bestimmung von nucleinsäure-amplifizierungsprodukten - Google Patents

Quantitatitative bestimmung von nucleinsäure-amplifizierungsprodukten

Info

Publication number
DE69935314D1
DE69935314D1 DE69935314T DE69935314T DE69935314D1 DE 69935314 D1 DE69935314 D1 DE 69935314D1 DE 69935314 T DE69935314 T DE 69935314T DE 69935314 T DE69935314 T DE 69935314T DE 69935314 D1 DE69935314 D1 DE 69935314D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid amplification
amplification products
quantitative determination
quantitative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69935314T
Other languages
English (en)
Other versions
DE69935314T2 (de
Inventor
Rajesh Patel
Nurith Kurn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dade Behring Inc
Original Assignee
Dade Behring Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Inc filed Critical Dade Behring Inc
Publication of DE69935314D1 publication Critical patent/DE69935314D1/de
Application granted granted Critical
Publication of DE69935314T2 publication Critical patent/DE69935314T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69935314T 1998-02-18 1999-02-17 Quantitatitative bestimmung von nucleinsäure-amplifizierungsprodukten Expired - Fee Related DE69935314T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/025,639 US6365346B1 (en) 1998-02-18 1998-02-18 Quantitative determination of nucleic acid amplification products
US25639 1998-02-18
PCT/US1999/003426 WO1999042618A1 (en) 1998-02-18 1999-02-17 Quantitative determination of nucleic acid amplification products

Publications (2)

Publication Number Publication Date
DE69935314D1 true DE69935314D1 (de) 2007-04-12
DE69935314T2 DE69935314T2 (de) 2007-11-22

Family

ID=21827235

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69935314T Expired - Fee Related DE69935314T2 (de) 1998-02-18 1999-02-17 Quantitatitative bestimmung von nucleinsäure-amplifizierungsprodukten

Country Status (6)

Country Link
US (3) US6365346B1 (de)
EP (1) EP1055005B1 (de)
JP (1) JP2002504350A (de)
DE (1) DE69935314T2 (de)
ES (1) ES2283124T3 (de)
WO (1) WO1999042618A1 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
BR0014182A (pt) 1999-09-13 2002-05-21 Nugen Technologies Inc Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas
AT409383B (de) * 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe
US6602665B2 (en) 2000-03-31 2003-08-05 Whitehead Institute For Biomedical Research Referenced amplification of small quantities of RNA
JP4796259B2 (ja) * 2000-05-04 2011-10-19 シーメンス・ヘルスケア・ダイアグノスティックス・プロダクツ・ゲーエムベーハー 複数のアナライトの検出において使用するための組成物
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
JP4963142B2 (ja) * 2000-12-14 2012-06-27 学校法人慶應義塾 遺伝子型と表現型の対応付け分子とその構成要素、および対応付け分子の製造方法と利用方法
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
ATE361996T1 (de) 2001-03-09 2007-06-15 Nugen Technologies Inc Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
US6818417B2 (en) * 2002-08-06 2004-11-16 Nalco Company Method for locating hidden microorganism contaminated surfaces in industrial water systems
US20040096829A1 (en) * 2002-11-14 2004-05-20 Allaire Normand E. Absolute quantitation of nucleic acids by RT-PCR
EP1567675A4 (de) 2002-11-21 2006-05-10 Epict Technologies Verfahren zur verwendung von primern,die für einen strang eines doppelsträngigen promotors codieren
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
WO2005065321A2 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
KR101076578B1 (ko) * 2004-06-23 2011-10-24 더 보드 오브 리젠츠 오브 더 유니버시티 오브 텍사스 시스템 2입자 착물을 이용한 생물학적 분자의 검출을 위한 방법 및조성물
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP1934374B1 (de) * 2005-10-05 2011-08-10 Roche Diagnostics GmbH Nicht-fluoreszente energieübertragung
EP1953541B1 (de) * 2005-11-16 2014-05-07 Mitsubishi Gas Chemical Company, Inc. VERFAHREN ZUR BESTIMMUNG der Menge an DOPPELSTRÄNGIGER DNA
US7226752B1 (en) * 2006-01-19 2007-06-05 Avago Technologies General Ip (Singapore) Pte. Ltd. Methods for detecting an analyte in a sample
WO2009006438A2 (en) 2007-06-29 2009-01-08 Epicentre Technologies Corporation Copy dna and sense rna
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
US9347947B2 (en) * 2009-03-12 2016-05-24 Siemens Healthcare Diagnostics Inc. Immunoassays employing non-particulate chemiluminescent reagent
EP2501716B1 (de) 2009-11-19 2015-01-21 Solis Biodyne Zusammensetzungen zur erhöhung der Polypeptidstabilität und -aktivität sowie zugehörige Verfahren
EP2600901B1 (de) 2010-08-06 2019-03-27 ModernaTX, Inc. Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung
HRP20220796T1 (hr) 2010-10-01 2022-10-14 ModernaTX, Inc. Ribonukleinske kiseline koje sadrže n1-metil-pseudouracil i njihove uporabe
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP3492109B1 (de) 2011-10-03 2020-03-04 ModernaTX, Inc. Modifizierte nukleoside, nukleotide und nukleinsäuren und verwendungen davon
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
RS63244B1 (sr) 2011-12-16 2022-06-30 Modernatx Inc Kompozicije modifikovane mrna
EP4372084A3 (de) 2012-01-26 2024-08-14 Tecan Genomics, Inc. Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und hocheffizienten erzeugung einer bibliothek
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
WO2013151664A1 (en) 2012-04-02 2013-10-10 modeRNA Therapeutics Modified polynucleotides for the production of proteins
US9303079B2 (en) 2012-04-02 2016-04-05 Moderna Therapeutics, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP4026912A1 (de) 2012-05-10 2022-07-13 The General Hospital Corporation Verfahren zur bestimmung einer nukleotidsequenz
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
PL2922554T3 (pl) 2012-11-26 2022-06-20 Modernatx, Inc. Na zmodyfikowany na końcach
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
EP3052106A4 (de) 2013-09-30 2017-07-19 ModernaTX, Inc. Polynukleotide zur codierung immunmodulierender polypeptide
SG11201602503TA (en) 2013-10-03 2016-04-28 Moderna Therapeutics Inc Polynucleotides encoding low density lipoprotein receptor
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CA2938080A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
JP6803327B2 (ja) 2014-08-06 2020-12-23 ニューゲン テクノロジーズ， インコーポレイテッド 標的化されたシークエンシングからのデジタル測定値
WO2017147486A1 (en) * 2016-02-26 2017-08-31 Alere San Diego Inc. Redox labeled oligonucleotide probes and their use
AU2017328953B2 (en) 2016-09-15 2023-09-14 Archerdx, Llc Methods of nucleic acid sample preparation for analysis of cell-free DNA
EP3933039A1 (de) 2016-09-15 2022-01-05 ArcherDX, LLC Verfahren für die zubereitung von nukleinsäureproben
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
WO2021183941A1 (en) * 2020-03-13 2021-09-16 Uh-Oh Labs Inc. Looped primer and loop-de-loop method for detecting target nucleic acid

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193983A (en) 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
US4766062A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4853327A (en) * 1985-07-10 1989-08-01 Molecular Diagnostics, Inc. Enhanced phthalazinedione chemiluminescence
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US5635347A (en) * 1986-04-30 1997-06-03 Igen, Inc. Rapid assays for amplification products
US4891324A (en) 1987-01-07 1990-01-02 Syntex (U.S.A.) Inc. Particle with luminescer for assays
US5017473A (en) 1987-05-26 1991-05-21 Becton, Dickinson And Company Homogeneous chemiluminescence immunoassay using a light absorbing material
US5030557A (en) * 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
JP2681061B2 (ja) * 1988-06-08 1997-11-19 ロンドン・ダイアグノスティック・インコーポレーテッド 検出可能なシグナルのセンシタイザー誘導発生を利用したアッセイ
US5656207A (en) 1989-06-24 1997-08-12 Gen Probe Incorporated Detecting or quantifying multiple analytes using labelling techniques
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
CA2037349C (en) 1990-03-26 2008-06-17 James G. Wetmur Branch migration of nucleotides
AU9115891A (en) 1990-11-14 1992-06-11 Siska Diagnostics, Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
US5340716A (en) 1991-06-20 1994-08-23 Snytex (U.S.A.) Inc. Assay method utilizing photoactivated chemiluminescent label
EP0515194B1 (de) 1991-05-22 2001-10-31 Dade Behring Marburg GmbH Bestimmungsmethoden unter Verwendung von induzierter Lumineszenz
ES2214472T3 (es) * 1991-08-02 2004-09-16 Biomerieux B.V. Cuantificacion de acidos nucleicos.
DE69231853T2 (de) * 1991-11-07 2001-09-13 Nanotronics, Inc. Hybridisierung von mit chromophoren und fluorophoren konjugierten polynukleotiden zur erzeugung eines donor-zu-donor energietransfersystems
JP2788786B2 (ja) 1991-11-15 1998-08-20 イゲン，インコーポレーテッド 増幅生成物の迅速アッセイ
EP1752545A3 (de) * 1991-12-23 2007-12-26 Novartis Vaccines and Diagnostics, Inc. HIV Sonden für ein Sandwich-Hybridisierungsverfahren in Lösung
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
ATE231920T1 (de) 1992-03-11 2003-02-15 Dana Farber Cancer Inst Inc Methode um mrna zu klonieren
WO1993022461A1 (en) * 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
EP0653066B1 (de) * 1992-07-31 1998-01-28 BEHRINGWERKE Aktiengesellschaft Photoaktivierbare chemiluminizierende matrices
ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
US5445933A (en) 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
DE69432419T2 (de) 1993-09-22 2004-01-08 Igen International, Inc. Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
US5624803A (en) 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
DE69535240T2 (de) * 1994-10-28 2007-06-06 Gen-Probe Inc., San Diego Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen
WO1997021832A1 (de) 1995-12-08 1997-06-19 Evotec Biosystems Gmbh Verfahren zur bestimmung von nukleinsäuremolekülen in niedriger konzentration
EP0819181B1 (de) 1996-02-01 2002-08-07 Aventis Pharmaceuticals, Inc. Positiv kontrollen zur verwendung bei polynukleotid - vervielfältigungen
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products

Also Published As

Publication number Publication date
EP1055005B1 (de) 2007-02-28
US20030175785A1 (en) 2003-09-18
DE69935314T2 (de) 2007-11-22
US6365346B1 (en) 2002-04-02
US6573054B2 (en) 2003-06-03
EP1055005A1 (de) 2000-11-29
US20020182620A1 (en) 2002-12-05
WO1999042618A1 (en) 1999-08-26
JP2002504350A (ja) 2002-02-12
ES2283124T3 (es) 2007-10-16

Similar Documents

Publication Publication Date Title
DE69935314D1 (de) Quantitatitative bestimmung von nucleinsäure-amplifizierungsprodukten
DE69829328D1 (de) Strangverdrängungsamplifikation von RNA
DE69637065D1 (de) Die bestimmung von nukleinsäuren und nukleinsäure-einheiten
DE69636843D1 (de) Nachweis von Nukleinsäure-Änderungen
HUP0105052A3 (en) Methods of nucleic acid amplification and sequencing
DE69739128D1 (de) Invasive Spaltung von Nukleinsäuren
TR199800046A3 (tr) Aktif maddeler yaratilmasi için nükleik asit olusumu.
DE69836885D1 (de) Nachweis von Nukleinsäuren durch Fluoreszenz quenching
DE69832490D1 (de) Vervielfältigung von nukleinsäuren
DE69635744D1 (de) Modifizierte Nukleinsäuresonden
FI960469A0 (fi) DNA:ta sekvenssoivia entsyymejä
DE69933283D1 (de) Photoaktivierbare nukleinsäure-derivate
DK1133352T3 (da) Biologisk assay-teknik
DE59508908D1 (de) Elektrochemischer Enzymbiosensor
DE59701329D1 (de) Verwendung von naphthalinsulfonsäure-formaldehyd-kondensationsprodukten als trocknungshilfsmittel
ATE264069T1 (de) Verstärkung von nahrungsmitteln
DE69839679D1 (de) Handlung von verschiedenen protokollen
DE69637579D1 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
DE69921311D1 (de) Vervielfältigung von nukleinsäuren
DE69731646D1 (de) Verabreichung von nukleinsäurepartikeln
PT878467E (pt) Acidos 6-aminotetra-hidroisoquinolinacarboxilicos e 7-aminotetra-hidroisoquinolinacarboxilicos substituidos
DE59712415D1 (de) Verfahren zur amplifikation von nukleinsäure
DK1330525T3 (da) NOD2-nukleinsyrer og proteiner
DE69221641D1 (de) Quantitativer nachweis von nukleinsäure-amplifikationsprodukten
DE60022125D1 (de) Tiefstkühlen von Waren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee