DE69924873D1 - Cytochrom p450 monooxygenase und nadph-cytochrom p450-oxidoreduktase gene und proteine mit bezug zum omega-hydroxylase-komplex in candida tropicalis und dazu gehörigen verfahren - Google Patents

Cytochrom p450 monooxygenase und nadph-cytochrom p450-oxidoreduktase gene und proteine mit bezug zum omega-hydroxylase-komplex in candida tropicalis und dazu gehörigen verfahren

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Publication number
DE69924873D1
DE69924873D1 DE69924873T DE69924873T DE69924873D1 DE 69924873 D1 DE69924873 D1 DE 69924873D1 DE 69924873 T DE69924873 T DE 69924873T DE 69924873 T DE69924873 T DE 69924873T DE 69924873 D1 DE69924873 D1 DE 69924873D1
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DE
Germany
Prior art keywords
methods
genes
enzymes
cytochrom
monooxygenase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69924873T
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English (en)
Other versions
DE69924873T2 (de
Inventor
Ron Wilson
L Craft
Dudley Eirich
Mark Eshoo
Krishna M Madduri
Cathy A Cornett
A Brenner
Maria Tang
C Loper
Martin Gleeson
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Cognis Corp
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Cognis Corp
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Publication of DE69924873D1 publication Critical patent/DE69924873D1/de
Application granted granted Critical
Publication of DE69924873T2 publication Critical patent/DE69924873T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/40—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69924873T 1998-10-05 1999-09-10 Cytochrom p450 monooxygenase und nadph-cytochrom p450-oxidoreduktase gene und proteine mit bezug zum omega-hydroxylase-komplex in candida tropicalis und dazu gehörigen verfahren Expired - Lifetime DE69924873T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10309998P 1998-10-05 1998-10-05
US103099P 1998-10-05
US12355599P 1999-03-10 1999-03-10
US123555P 1999-03-10
PCT/US1999/020797 WO2000020566A2 (en) 1998-10-05 1999-09-10 Cytochrome p450 monooxygenase and nadph cytochrome p450 oxidoreductase genes and proteins related to the omega hydroxylase complex of candida tropicalis and methods relating thereto

Publications (2)

Publication Number Publication Date
DE69924873D1 true DE69924873D1 (de) 2005-05-25
DE69924873T2 DE69924873T2 (de) 2006-03-02

Family

ID=26800090

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69941604T Expired - Lifetime DE69941604D1 (de) 1998-10-05 1999-09-10 Cytochrom P450 Monooxygenase Gen und Protein des Omega-Hydroxylase-Komplexes von Candida Tropicalis und darauf bezogene Verfahren
DE69941603T Expired - Lifetime DE69941603D1 (de) 1998-10-05 1999-09-10 Cytochrom P450 Monooxygenase Gen und Protein des omega-Hydroxylase-Komplexes von Candida tropicalis und darauf bezogene Verfahren
DE69924873T Expired - Lifetime DE69924873T2 (de) 1998-10-05 1999-09-10 Cytochrom p450 monooxygenase und nadph-cytochrom p450-oxidoreduktase gene und proteine mit bezug zum omega-hydroxylase-komplex in candida tropicalis und dazu gehörigen verfahren

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE69941604T Expired - Lifetime DE69941604D1 (de) 1998-10-05 1999-09-10 Cytochrom P450 Monooxygenase Gen und Protein des Omega-Hydroxylase-Komplexes von Candida Tropicalis und darauf bezogene Verfahren
DE69941603T Expired - Lifetime DE69941603D1 (de) 1998-10-05 1999-09-10 Cytochrom P450 Monooxygenase Gen und Protein des omega-Hydroxylase-Komplexes von Candida tropicalis und darauf bezogene Verfahren

Country Status (7)

Country Link
EP (12) EP1148133A3 (de)
AT (3) ATE447028T1 (de)
AU (1) AU5917799A (de)
CA (2) CA2349868C (de)
DE (3) DE69941604D1 (de)
ES (1) ES2243075T3 (de)
WO (1) WO2000020566A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7610200A (en) 1999-09-30 2001-05-10 Cognis Corporation Improved fermentation process
US20020061566A1 (en) 2000-03-20 2002-05-23 Eirich L. Dudley Biooxidation capabilities of candida sp
US6660505B2 (en) 2000-06-22 2003-12-09 Cognis Corporation Isolation of carboxylic acids from fermentation broth
US6673613B2 (en) * 2000-07-26 2004-01-06 Cognis Corporation Use of CYP52A2A promoter to increase gene expression in yeast
WO2003031596A2 (en) * 2001-10-12 2003-04-17 Cognis Corporation Production of a highly active, soluble form of the cytochrome p450 reductase (cpr a) from candida tropicalis
AU2003247411A1 (en) 2002-05-23 2003-12-12 Cognis Corporation NON-REVERTIBLE Beta-OXIDATION BLOCKED CANDIDA TROPICALIS
US6777213B2 (en) 2002-10-29 2004-08-17 Cognis Corporation Isolation of carboxylic acids from fermentation broth
CN100591754C (zh) * 2005-10-14 2010-02-24 中国科学院大连化学物理研究所 一种共表达cyp和cpr的酵母系统
DE102007060705A1 (de) 2007-12-17 2009-06-18 Evonik Degussa Gmbh ω-Aminocarbonsäuren oder ihre Lactame, herstellende, rekombinante Zellen
DE102009046626A1 (de) 2009-11-11 2011-05-12 Evonik Degussa Gmbh Candida tropicalis Zellen und deren Verwendung
DE102010015807A1 (de) 2010-04-20 2011-10-20 Evonik Degussa Gmbh Biokatalytisches Oxidationsverfahren mit alkL-Genprodukt
EP2944697A1 (de) 2014-05-13 2015-11-18 Evonik Degussa GmbH Verfahren zur Herstellung von Nylon
WO2017102952A1 (en) 2015-12-17 2017-06-22 Evonik Degussa Gmbh A genetically modified acetogenic cell
MY187786A (en) 2016-07-27 2021-10-22 Evonik Operations Gmbh N-acetyl homoserine
US20200010862A1 (en) * 2018-07-06 2020-01-09 Cathay Biotech Inc. Long-chain dibasic acid with low content of fatty acid impurity and a method of producing the same

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1365689A (en) 1970-12-26 1974-09-04 Hasegawa T Co Ltd Process for the production of carboxylic acids
DE2140133C2 (de) 1971-08-10 1984-08-09 Ajinomoto Co., Inc., Tokio Verfahren zur mikrobiologischen Herstellung von α,ω-n-Alkandicarbonsäuren
JPS5543759B2 (de) 1972-06-28 1980-11-07
US3975234A (en) 1975-07-03 1976-08-17 Phillips Petroleum Company Microbial production of dicarboxylic acids
JPS5946598B2 (ja) 1977-12-14 1984-11-13 株式会社バイオリサ−チセンタ− 微生物による長鎖脂肪酸類の製造法
JPS5539778A (en) 1978-09-14 1980-03-19 Baiorisaac Center:Kk Microbial preparation of carboxylic acid composed mainly of omega,omega-1-dihydroxy acid
JPS5592691A (en) 1978-12-28 1980-07-14 Baiorisaac Center:Kk Purification of dicarboxylic acid produced by fermentation
US4339536A (en) 1979-06-08 1982-07-13 Nippon Mining Co., Ltd. Process for the preparation of long-chain dicarboxylic acids by fermentation
JPS5765194A (en) 1980-10-09 1982-04-20 Daicel Chem Ind Ltd Microbial preparation of unsaturated dicarboxylic acid
US5254466A (en) 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
WO1991014781A1 (en) * 1990-03-19 1991-10-03 Henkel Research Corporation METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS)
US6331420B1 (en) 1998-05-01 2001-12-18 C. Ron Wilson Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto
US6503734B1 (en) 2000-07-26 2003-01-07 Cognis Corporation Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto
US7388084B2 (en) * 2002-08-16 2008-06-17 Cognis Corporation Promoter motifs in Candida tropicalis
DE102007042592A1 (de) 2007-09-07 2009-03-26 Siemens Medical Instruments Pte. Ltd. Übertragungseinrichtung für eine Hörvorrichtung mit Folienleiterschirmung und eigengeschirmte Spule

Also Published As

Publication number Publication date
EP1148135A2 (de) 2001-10-24
EP1162268A3 (de) 2002-01-16
DE69941603D1 (de) 2009-12-10
EP1148134A3 (de) 2002-01-16
AU5917799A (en) 2000-04-26
EP1148132A3 (de) 2002-01-16
EP1148131A2 (de) 2001-10-24
EP1162268A2 (de) 2001-12-12
EP1148133A2 (de) 2001-10-24
EP1148138A2 (de) 2001-10-24
EP1148130A2 (de) 2001-10-24
CA2653985A1 (en) 2000-04-13
WO2000020566A9 (en) 2000-08-24
EP1148136A2 (de) 2001-10-24
EP1148136A3 (de) 2002-01-16
EP1148137A3 (de) 2002-01-16
EP1148143A2 (de) 2001-10-24
EP1148143A3 (de) 2002-01-16
EP1148131A3 (de) 2002-01-16
CA2349868A1 (en) 2000-04-13
EP1135506A2 (de) 2001-09-26
EP1148132A2 (de) 2001-10-24
EP1148134B1 (de) 2009-10-28
ATE447028T1 (de) 2009-11-15
EP1148135A3 (de) 2002-01-16
DE69924873T2 (de) 2006-03-02
ES2243075T3 (es) 2005-11-16
EP1148131B1 (de) 2009-10-28
DE69941604D1 (de) 2009-12-10
WO2000020566A2 (en) 2000-04-13
ATE293694T1 (de) 2005-05-15
EP1148130A3 (de) 2002-01-16
ATE447029T1 (de) 2009-11-15
CA2349868C (en) 2010-07-20
EP1148136B1 (de) 2012-12-05
WO2000020566A3 (en) 2000-10-12
EP1148137A2 (de) 2001-10-24
EP1148138A3 (de) 2002-01-16
EP1148134A2 (de) 2001-10-24
EP1148133A3 (de) 2002-01-16
EP1135506B1 (de) 2005-04-20

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