DE69920113D1 - Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie - Google Patents

Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie

Info

Publication number
DE69920113D1
DE69920113D1 DE69920113T DE69920113T DE69920113D1 DE 69920113 D1 DE69920113 D1 DE 69920113D1 DE 69920113 T DE69920113 T DE 69920113T DE 69920113 T DE69920113 T DE 69920113T DE 69920113 D1 DE69920113 D1 DE 69920113D1
Authority
DE
Germany
Prior art keywords
antibiotic
system based
escherichia coli
vector system
coli host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69920113T
Other languages
English (en)
Other versions
DE69920113T2 (de
Inventor
Erhard Kopetzki
Christian Schantz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE69920113D1 publication Critical patent/DE69920113D1/de
Publication of DE69920113T2 publication Critical patent/DE69920113T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medical Treatment And Welfare Office Work (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69920113T 1998-07-15 1999-07-08 Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie Expired - Lifetime DE69920113T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP98113156 1998-07-15
EP98113156 1998-07-15
EP98119078 1998-10-09
EP98119078 1998-10-09

Publications (2)

Publication Number Publication Date
DE69920113D1 true DE69920113D1 (de) 2004-10-21
DE69920113T2 DE69920113T2 (de) 2005-12-15

Family

ID=26149433

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69920113T Expired - Lifetime DE69920113T2 (de) 1998-07-15 1999-07-08 Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie

Country Status (9)

Country Link
US (1) US6291245B1 (de)
JP (1) JP3801389B2 (de)
KR (1) KR100355963B1 (de)
CN (1) CN1196792C (de)
AT (1) ATE276371T1 (de)
AU (1) AU720627B2 (de)
CA (1) CA2274982C (de)
DE (1) DE69920113T2 (de)
ES (1) ES2227938T3 (de)

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AU2004293810B2 (en) * 2003-11-19 2010-07-01 Pelican Technology Holdings, Inc. Improved protein expression systems
DE10360483B4 (de) * 2003-12-22 2007-11-15 Scil Proteins Gmbh Expressionsvektor und dessen Verwendung
EP1885847B1 (de) * 2005-05-26 2011-07-06 Cytos Biotechnology AG Skalierbares fermentationsverfahren
NZ567190A (en) * 2005-10-06 2012-07-27 Dompe Pha R Ma Spa Res & Mfg Method of producing a recombinant protein comprising transforming an E. Coli host cell lacking a gene encoding pyrC with a vector comprising a gene encoding the recombinant protein and a gene encoding E. coli pyrC
JP2009545328A (ja) 2006-08-03 2009-12-24 ウィスコンシン アルムニ リサーチ ファウンデーション ベクター系
WO2010132341A2 (en) 2009-05-11 2010-11-18 Pfenex, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
ES2758782T3 (es) 2010-06-01 2020-05-06 Dsm Ip Assets Bv Extracción de lípido de células y productos del mismo
TW201215394A (en) 2010-08-30 2012-04-16 Hoffmann La Roche Tetranectin-apolipoprotein A-I, lipid particles containing it and its use
BR112013004397A2 (pt) 2010-08-30 2017-06-27 Hoffmann La Roche método para produzir uma partícula de lipídio, a partícula de lipídio em si e seu uso
US8877895B2 (en) 2010-08-30 2014-11-04 Hoffmann-La Roche Inc. Prokaryotic expression construct
CN103068967B (zh) * 2010-08-30 2016-05-04 霍夫曼-拉罗奇有限公司 碱性进料
JP2013538205A (ja) 2010-08-30 2013-10-10 エフ．ホフマン−ラ ロシュ アーゲー テトラネクチン−アポリポタンパク質ａ−ｉ粒子を産生するための方法、それを用いて得られる脂質粒子、及びその使用
RU2013113723A (ru) 2010-09-14 2014-10-20 Ф.Хоффманн-Ля Рош Аг Гибридный полипептид с серпиновым "пальцем"
WO2012146630A1 (en) 2011-04-29 2012-11-01 F. Hoffmann-La Roche Ag N-terminal acylated polypeptides, methods for their production and uses thereof
CA2838070A1 (en) 2011-08-25 2013-02-28 F. Hoffmann-La Roche Ag Shortened tetranectin-apolipoprotein a-i fusion protein, a lipid particle containing it, and uses thereof
WO2013127752A1 (en) 2012-02-29 2013-09-06 F. Hoffmann-La Roche Ag Method for reduction of 1->3 reading frame shifts
CN104136459A (zh) 2012-02-29 2014-11-05 霍夫曼-拉罗奇有限公司 减少1-＞2读框移位的方法
US20150044718A1 (en) 2012-02-29 2015-02-12 Hoffmann-La Roche Inc. On-column enzymatic cleavage
RU2014143029A (ru) 2012-03-26 2016-05-20 Проньютриа, Инк. Заряженные питательные белки и способы их применения
US9700071B2 (en) 2012-03-26 2017-07-11 Axcella Health Inc. Nutritive fragments, proteins and methods
CN104364259A (zh) 2012-03-26 2015-02-18 普罗努塔利亚公司 营养性片段、蛋白质和方法
US9605040B2 (en) 2012-03-26 2017-03-28 Axcella Health Inc. Nutritive proteins and methods
CA2865676C (en) 2012-04-17 2020-03-10 Stefan Klostermann Method for the expression of polypeptides using modified nucleic acids
SG11201502538TA (en) 2012-11-08 2015-05-28 Hoffmann La Roche Her3 antigen binding proteins binding to the beta-hairpin of her3
WO2014128135A1 (en) * 2013-02-22 2014-08-28 F. Hoffmann-La Roche Ag Use of an amino acid auxotrophy cured prokaryotic strain for the recombinant production of a polypeptide
SG11201601999UA (en) 2013-09-25 2016-04-28 Pronutria Inc Compositions and formulations for prevention and treatment of diabetes and obesity, and methods of production and use thereof in glucose and caloric control
CA2926215A1 (en) 2013-10-06 2015-04-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Modified pseudomonas exotoxin a
WO2016004024A1 (en) 2014-07-02 2016-01-07 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
EP3184547A1 (de) 2015-10-29 2017-06-28 F. Hoffmann-La Roche AG Anti-tpbg-antikörper und verfahren zur verwendung
WO2017097383A1 (de) * 2015-12-11 2017-06-15 Wacker Chemie Ag Mikroorganismenstamm und verfahren zur antibiotikafreien, fermentativen herstellung von niedermolekularen substanzen und proteinen
NZ754510A (en) * 2016-12-20 2024-02-23 Univ Basel Virulence attenuated bacteria based protein delivery
US11421239B2 (en) * 2017-07-21 2022-08-23 Conagen Inc. Plasmid addiction system to drive desired gene expression
EP3584316B1 (de) 2018-06-20 2024-04-03 Eligo Bioscience Bakterielles freisetzungsvehikel, verfahren zur herstellung und verwendungen davon
JP6583759B1 (ja) * 2018-11-22 2019-10-02 勝 枦 Ｄｎａを改変したり、新たに核酸を導入した細胞を抗生物質を利用せずに確認、選抜する方法
CN111635879B (zh) * 2019-03-01 2022-04-22 中国科学院天津工业生物技术研究所 L-赖氨酸高产菌株及其构建方法和应用
EP4077363A1 (de) 2019-12-20 2022-10-26 F. Hoffmann-La Roche AG Il-37-fusionsproteine und verwendungen davon
US11746352B2 (en) 2019-12-30 2023-09-05 Eligo Bioscience Microbiome modulation of a host by delivery of DNA payloads with minimized spread
WO2022144382A1 (en) 2020-12-30 2022-07-07 Eligo Bioscience Chimeric receptor binding proteins resistant to proteolytic degradation
IL308147A (en) 2021-05-12 2023-12-01 Eligo Bioscience Production of bacterial cells and their use in production methods

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Also Published As

Publication number Publication date
JP3801389B2 (ja) 2006-07-26
CA2274982A1 (en) 2000-01-15
ATE276371T1 (de) 2004-10-15
CN1241635A (zh) 2000-01-19
ES2227938T3 (es) 2005-04-01
DE69920113T2 (de) 2005-12-15
CA2274982C (en) 2005-01-04
US6291245B1 (en) 2001-09-18
KR20000011710A (ko) 2000-02-25
AU720627B2 (en) 2000-06-08
CN1196792C (zh) 2005-04-13
AU4011599A (en) 2000-02-10
KR100355963B1 (ko) 2002-10-19
JP2000050888A (ja) 2000-02-22

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