DE69919143T2 - Nukleinsäure hybridisierung-testverfahren in lösung - Google Patents
Nukleinsäure hybridisierung-testverfahren in lösung Download PDFInfo
- Publication number
- DE69919143T2 DE69919143T2 DE69919143T DE69919143T DE69919143T2 DE 69919143 T2 DE69919143 T2 DE 69919143T2 DE 69919143 T DE69919143 T DE 69919143T DE 69919143 T DE69919143 T DE 69919143T DE 69919143 T2 DE69919143 T2 DE 69919143T2
- Authority
- DE
- Germany
- Prior art keywords
- label
- capture
- waveguide
- target polynucleotide
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000007899 nucleic acid hybridization Methods 0.000 title 1
- 238000010998 test method Methods 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 124
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 124
- 239000002157 polynucleotide Substances 0.000 claims abstract description 124
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 65
- 238000003556 assay Methods 0.000 claims abstract description 51
- 238000004020 luminiscence type Methods 0.000 claims abstract description 43
- 230000003287 optical effect Effects 0.000 claims abstract description 36
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 29
- 201000010099 disease Diseases 0.000 claims abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 205
- 239000004606 Fillers/Extenders Substances 0.000 claims description 105
- 230000000295 complement effect Effects 0.000 claims description 53
- 238000001514 detection method Methods 0.000 claims description 32
- 230000027455 binding Effects 0.000 claims description 31
- 108020004414 DNA Proteins 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 230000003993 interaction Effects 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 11
- 125000003729 nucleotide group Chemical group 0.000 claims description 11
- 244000052769 pathogen Species 0.000 claims description 10
- 238000002372 labelling Methods 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 241000700605 Viruses Species 0.000 claims description 5
- 125000005647 linker group Chemical group 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000012736 aqueous medium Substances 0.000 claims description 2
- 238000003745 diagnosis Methods 0.000 claims description 2
- 238000011835 investigation Methods 0.000 claims 1
- 239000010410 layer Substances 0.000 description 46
- 230000005284 excitation Effects 0.000 description 21
- 230000035945 sensitivity Effects 0.000 description 19
- 239000000243 solution Substances 0.000 description 18
- 238000009396 hybridization Methods 0.000 description 17
- 230000003321 amplification Effects 0.000 description 12
- 238000003199 nucleic acid amplification method Methods 0.000 description 12
- 108091034117 Oligonucleotide Proteins 0.000 description 11
- 230000008878 coupling Effects 0.000 description 11
- 238000010168 coupling process Methods 0.000 description 11
- 238000005859 coupling reaction Methods 0.000 description 11
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 10
- 238000005259 measurement Methods 0.000 description 10
- 238000005406 washing Methods 0.000 description 10
- 241001668536 Oculimacula yallundae Species 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 239000002609 medium Substances 0.000 description 8
- 238000011002 quantification Methods 0.000 description 8
- 230000035772 mutation Effects 0.000 description 7
- 239000012071 phase Substances 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 238000004925 denaturation Methods 0.000 description 6
- 230000036425 denaturation Effects 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 239000000975 dye Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- BPSIOYPQMFLKFR-UHFFFAOYSA-N trimethoxy-[3-(oxiran-2-ylmethoxy)propyl]silane Chemical compound CO[Si](OC)(OC)CCCOCC1CO1 BPSIOYPQMFLKFR-UHFFFAOYSA-N 0.000 description 5
- 241001290235 Ceratobasidium cereale Species 0.000 description 4
- 241000701022 Cytomegalovirus Species 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 241000711549 Hepacivirus C Species 0.000 description 4
- 241000725303 Human immunodeficiency virus Species 0.000 description 4
- JGFZNNIVVJXRND-UHFFFAOYSA-N N,N-Diisopropylethylamine (DIPEA) Chemical compound CCN(C(C)C)C(C)C JGFZNNIVVJXRND-UHFFFAOYSA-N 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108090001008 Avidin Proteins 0.000 description 3
- 241001157813 Cercospora Species 0.000 description 3
- 239000004593 Epoxy Substances 0.000 description 3
- 241000700721 Hepatitis B virus Species 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 241000736122 Parastagonospora nodorum Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000007846 asymmetric PCR Methods 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000009871 nonspecific binding Effects 0.000 description 3
- 238000002515 oligonucleotide synthesis Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 230000035515 penetration Effects 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 244000000003 plant pathogen Species 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000001509 sodium citrate Substances 0.000 description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 2
- 241000190150 Bipolaris sorokiniana Species 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- 241000223195 Fusarium graminearum Species 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- 241001459558 Monographella nivalis Species 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- 241001533598 Septoria Species 0.000 description 2
- 229910010413 TiO 2 Inorganic materials 0.000 description 2
- -1 TiO 2 Chemical class 0.000 description 2
- 108020005202 Viral DNA Proteins 0.000 description 2
- 241001360088 Zymoseptoria tritici Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011067 equilibration Methods 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical class O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910044991 metal oxide Inorganic materials 0.000 description 2
- 150000004706 metal oxides Chemical class 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229940078552 o-xylene Drugs 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 125000004424 polypyridyl Polymers 0.000 description 2
- 238000011533 pre-incubation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000007142 ring opening reaction Methods 0.000 description 2
- 150000003303 ruthenium Chemical class 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 125000003396 thiol group Chemical group [H]S* 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- VEPOHXYIFQMVHW-XOZOLZJESA-N 2,3-dihydroxybutanedioic acid (2S,3S)-3,4-dimethyl-2-phenylmorpholine Chemical compound OC(C(O)C(O)=O)C(O)=O.C[C@H]1[C@@H](OCCN1C)c1ccccc1 VEPOHXYIFQMVHW-XOZOLZJESA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- SIDCPPUYOKZTEY-UHFFFAOYSA-K 2-pyridin-2-ylpyridine;ruthenium(3+);trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Ru+3].N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1 SIDCPPUYOKZTEY-UHFFFAOYSA-K 0.000 description 1
- BCNPOXJWLYPAGO-UHFFFAOYSA-K 4,7-diphenyl-1,10-phenanthroline;ruthenium(3+);trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Ru+3].C1=CC=CC=C1C1=CC=NC2=C1C=CC1=C(C=3C=CC=CC=3)C=CN=C21.C1=CC=CC=C1C1=CC=NC2=C1C=CC1=C(C=3C=CC=CC=3)C=CN=C21.C1=CC=CC=C1C1=CC=NC2=C1C=CC1=C(C=3C=CC=CC=3)C=CN=C21 BCNPOXJWLYPAGO-UHFFFAOYSA-K 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- RQQVEUOMLMLXDY-UHFFFAOYSA-N 5-(4,4-dimethoxycyclohexa-1,5-dien-1-yl)-2-hydroxy-5,5-diphenylpentanoic acid Chemical compound C1=CC(OC)(OC)CC=C1C(CCC(O)C(O)=O)(C=1C=CC=CC=1)C1=CC=CC=C1 RQQVEUOMLMLXDY-UHFFFAOYSA-N 0.000 description 1
- CJIJXIFQYOPWTF-UHFFFAOYSA-N 7-hydroxycoumarin Natural products O1C(=O)C=CC2=CC(O)=CC=C21 CJIJXIFQYOPWTF-UHFFFAOYSA-N 0.000 description 1
- 241000050634 Aureobasidium zeae Species 0.000 description 1
- 241000228438 Bipolaris maydis Species 0.000 description 1
- 241000228439 Bipolaris zeicola Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 239000004970 Chain extender Substances 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 1
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000131448 Mycosphaerella Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 241000787361 Parastagonospora avenae Species 0.000 description 1
- PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 241001123567 Puccinia sorghi Species 0.000 description 1
- 241000190117 Pyrenophora tritici-repentis Species 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241000332749 Setosphaeria turcica Species 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 150000001217 Terbium Chemical class 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- CSUNYJDUAUTOOT-UHFFFAOYSA-K [Ru](Cl)(Cl)Cl.N1=CC=CC2=CC=C3C=CC=NC3=C12.N1=CC=CC2=CC=C3C=CC=NC3=C12.N1=CC=CC2=CC=C3C=CC=NC3=C12 Chemical compound [Ru](Cl)(Cl)Cl.N1=CC=CC2=CC=C3C=CC=NC3=C12.N1=CC=CC2=CC=C3C=CC=NC3=C12.N1=CC=CC2=CC=C3C=CC=NC3=C12 CSUNYJDUAUTOOT-UHFFFAOYSA-K 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000002318 adhesion promoter Substances 0.000 description 1
- MDBRABWLQUVUBW-UHFFFAOYSA-N aminosulfanylformic acid Chemical compound NSC(O)=O MDBRABWLQUVUBW-UHFFFAOYSA-N 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000011948 assay development Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- OMFRMAHOUUJSGP-IRHGGOMRSA-N bifenthrin Chemical compound C1=CC=C(C=2C=CC=CC=2)C(C)=C1COC(=O)[C@@H]1[C@H](\C=C(/Cl)C(F)(F)F)C1(C)C OMFRMAHOUUJSGP-IRHGGOMRSA-N 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000012677 causal agent Substances 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005401 electroluminescence Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000008713 feedback mechanism Effects 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 230000005021 gait Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 238000002444 silanisation Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical class C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 238000006557 surface reaction Methods 0.000 description 1
- UJMBCXLDXJUMFB-GLCFPVLVSA-K tartrazine Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)C1=NN(C=2C=CC(=CC=2)S([O-])(=O)=O)C(=O)C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 UJMBCXLDXJUMFB-GLCFPVLVSA-K 0.000 description 1
- 229960000943 tartrazine Drugs 0.000 description 1
- 235000012756 tartrazine Nutrition 0.000 description 1
- 239000004149 tartrazine Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 239000012815 thermoplastic material Substances 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229920006352 transparent thermoplastic Polymers 0.000 description 1
- ORHBXUUXSCNDEV-UHFFFAOYSA-N umbelliferone Chemical compound C1=CC(=O)OC2=CC(O)=CC=C21 ORHBXUUXSCNDEV-UHFFFAOYSA-N 0.000 description 1
- HFTAFOQKODTIJY-UHFFFAOYSA-N umbelliferone Natural products Cc1cc2C=CC(=O)Oc2cc1OCC=CC(C)(C)O HFTAFOQKODTIJY-UHFFFAOYSA-N 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9805935 | 1998-03-19 | ||
| GBGB9805935.5A GB9805935D0 (en) | 1998-03-19 | 1998-03-19 | Organic compounds |
| PCT/EP1999/001782 WO1999047705A1 (en) | 1998-03-19 | 1999-03-17 | Detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69919143D1 DE69919143D1 (de) | 2004-09-09 |
| DE69919143T2 true DE69919143T2 (de) | 2005-08-11 |
Family
ID=10828909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69919143T Expired - Lifetime DE69919143T2 (de) | 1998-03-19 | 1999-03-17 | Nukleinsäure hybridisierung-testverfahren in lösung |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20060188873A1 (enExample) |
| EP (1) | EP1064406B1 (enExample) |
| JP (1) | JP2002506984A (enExample) |
| AT (1) | ATE272721T1 (enExample) |
| AU (1) | AU3414599A (enExample) |
| DE (1) | DE69919143T2 (enExample) |
| GB (1) | GB9805935D0 (enExample) |
| WO (1) | WO1999047705A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001262178A1 (en) * | 2000-04-14 | 2001-10-30 | Zeptosens Ag | Grid-waveguide structure for reinforcing an excitation field and use thereof |
| US20030138208A1 (en) * | 2000-05-06 | 2003-07-24 | Michael Pawlak | Grating optical waveguide structure for multi-analyte determinations and the use thereof |
| US7158224B2 (en) | 2000-06-25 | 2007-01-02 | Affymetrix, Inc. | Optically active substrates |
| GB0106949D0 (en) * | 2001-03-20 | 2001-05-09 | Norchip As | Detection of mycobacteria |
| JP3448654B2 (ja) | 2001-11-22 | 2003-09-22 | 北陸先端科学技術大学院大学長 | バイオチップ、バイオチップアレイ、及びそれらを用いたスクリーニング方法 |
| JP2005519593A (ja) | 2002-01-18 | 2005-07-07 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | 平面導波路を使用する1塩基多型の検出法 |
| GB0507835D0 (en) * | 2005-04-18 | 2005-05-25 | Solexa Ltd | Method and device for nucleic acid sequencing using a planar wave guide |
| WO2007037282A1 (ja) * | 2005-09-27 | 2007-04-05 | Eisai R & D Management Co., Ltd. | 微小粒子に自己集合体を形成させる方法及び標的分析物の検出方法 |
| KR20100025328A (ko) * | 2008-08-27 | 2010-03-09 | 삼성전자주식회사 | 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법 |
| US9212995B2 (en) | 2009-03-02 | 2015-12-15 | Mbio Diagnostics, Inc. | System and method for detecting multiple molecules in one assay |
| US8331751B2 (en) | 2009-03-02 | 2012-12-11 | mBio Diagnositcs, Inc. | Planar optical waveguide with core of low-index-of-refraction interrogation medium |
| US9658222B2 (en) | 2009-03-02 | 2017-05-23 | Mbio Diagnostics, Inc. | Planar waveguide based cartridges and associated methods for detecting target analyte |
| US8300993B2 (en) | 2009-03-02 | 2012-10-30 | Mbio Diagnostics, Inc. | Waveguide with integrated lens |
| US9109793B2 (en) * | 2009-07-20 | 2015-08-18 | Crayola, Llc | Illuminated display unit |
| WO2012037369A1 (en) | 2010-09-15 | 2012-03-22 | Mbio Diagnostics, Inc. | System and method for detecting multiple molecules in one assay |
| WO2013129457A1 (ja) | 2012-02-27 | 2013-09-06 | 東レ株式会社 | 核酸の検出方法 |
| EP3908826A4 (en) * | 2019-01-08 | 2022-10-19 | Akoya Biosciences, Inc. | FLEXIBLE DETECTION SYSTEMS |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US5283174A (en) * | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
| US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| GB9119735D0 (en) * | 1991-09-16 | 1991-10-30 | Secr Defence | Gene probe biosensor method |
| US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| EP1890130A3 (en) * | 1995-05-12 | 2008-02-27 | Novartis AG | Sensor platform for the parallel detection of a plurality of analytes using evanescently excited luminescenes |
| AU4040097A (en) * | 1996-07-12 | 1998-02-09 | Tm Technologies, Inc. | Signal amplification method |
| US5832165A (en) * | 1996-08-28 | 1998-11-03 | University Of Utah Research Foundation | Composite waveguide for solid phase binding assays |
-
1998
- 1998-03-19 GB GBGB9805935.5A patent/GB9805935D0/en not_active Ceased
-
1999
- 1999-03-17 JP JP2000536887A patent/JP2002506984A/ja active Pending
- 1999-03-17 AT AT99915651T patent/ATE272721T1/de not_active IP Right Cessation
- 1999-03-17 DE DE69919143T patent/DE69919143T2/de not_active Expired - Lifetime
- 1999-03-17 AU AU34145/99A patent/AU3414599A/en not_active Abandoned
- 1999-03-17 WO PCT/EP1999/001782 patent/WO1999047705A1/en not_active Ceased
- 1999-03-17 EP EP99915651A patent/EP1064406B1/en not_active Expired - Lifetime
-
2005
- 2005-10-11 US US11/246,287 patent/US20060188873A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1064406B1 (en) | 2004-08-04 |
| JP2002506984A (ja) | 2002-03-05 |
| DE69919143D1 (de) | 2004-09-09 |
| WO1999047705A1 (en) | 1999-09-23 |
| ATE272721T1 (de) | 2004-08-15 |
| EP1064406A1 (en) | 2001-01-03 |
| AU3414599A (en) | 1999-10-11 |
| GB9805935D0 (en) | 1998-05-13 |
| US20060188873A1 (en) | 2006-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69919143T2 (de) | Nukleinsäure hybridisierung-testverfahren in lösung | |
| DE69637065T2 (de) | Die bestimmung von nukleinsäuren und nukleinsäure-einheiten | |
| DE69728017T2 (de) | Vorrichtungen und Verfahren zur Erkennung von mehreren Analyten in Proben | |
| DE69010506T2 (de) | Bestimmungsverfahren für biologische zielkomplexe auf der oberfläche eines biosensors. | |
| DE69331067T2 (de) | Verfahren und vorrichtung zum nachweis von nukleinsäure oder analysen unter verwendung der internen totalreflexion | |
| AT403961B (de) | Optochemisches messsystem mit einem fluoreszenzsensor | |
| DE60014762T2 (de) | Methode zum Nachweis von Ribonukleinsäuren | |
| DE69031665T2 (de) | Nachweis von Nukleinsäuresequenzen unter Verwendung von Fluoreszenz-Polarisation | |
| DE19844931C1 (de) | Verfahren zur DNS- oder RNS-Sequenzierung | |
| US20090087838A1 (en) | Analyte detection using autocatalytic chain reactions | |
| EP2376650B1 (de) | Nachweiskonjugat und verfahren zu polychromatischen analyse | |
| JP2009178171A (ja) | 複合混合物中における分析物の検出および定量のための方法 | |
| DE60213256T2 (de) | Verfahren zur bestimmung von mehrere analyten | |
| WO2018114674A1 (de) | Zweiteilige mediatorsonde | |
| DE69717601T2 (de) | Verfahren zur analyse von nukleinsäure-wiederholungssequenzen | |
| DE60015980T2 (de) | Biosensorsystem mit erhöhter empfindlichkeit mittels molekularer amplifikation des signals | |
| CN118638902A (zh) | 一种基于靶标诱导链位移的高灵敏度荧光适配传感器探针及其制备方法 | |
| DE10137342A1 (de) | Biosensor und Verfahren zum Erfassen von makromolekularen Biopolymeren mittels mindestens einer Einheit zum Immobilisieren von makromolekularen Biopolymeren | |
| DE102012203964B3 (de) | Verfahren und Kit zur Detektion von Nukleinsäuren | |
| EP1963441B1 (de) | Polyelektrolyt mono- und multischichten für optische signalwandler | |
| EP0912765B1 (de) | Bestimmung von analyten unter verwendung zweier markierungen | |
| DE102005029811B4 (de) | Oligonukleotidanordnungen, Verfahren zu deren Einsatz und deren Verwendung | |
| Chahar et al. | Biosensors for nucleic acid detection | |
| WO2003040679A2 (de) | Reversible bindung eines fluorophors an eine oberfläche zur detektion von ligat-ligand-assoziationsereingnissen durch fluoreszenz-quenchen | |
| CN101448956A (zh) | 用于dna-照相术的分子信标 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8328 | Change in the person/name/address of the agent |
Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: LUETJENS, H., DIPL.-CHEM. DR. RER. NAT., PAT.-ANW. |