DE69829976T2 - Verfahren zur herstellung von zellen - Google Patents
Verfahren zur herstellung von zellen Download PDFInfo
- Publication number
- DE69829976T2 DE69829976T2 DE69829976T DE69829976T DE69829976T2 DE 69829976 T2 DE69829976 T2 DE 69829976T2 DE 69829976 T DE69829976 T DE 69829976T DE 69829976 T DE69829976 T DE 69829976T DE 69829976 T2 DE69829976 T2 DE 69829976T2
- Authority
- DE
- Germany
- Prior art keywords
- cells
- competent
- transformation efficiency
- stored
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004519 manufacturing process Methods 0.000 title abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 92
- 230000009466 transformation Effects 0.000 claims abstract description 55
- 108020004414 DNA Proteins 0.000 claims abstract description 51
- 102000053602 DNA Human genes 0.000 claims abstract description 33
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 26
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 20
- 230000001131 transforming effect Effects 0.000 claims abstract description 11
- 210000004027 cell Anatomy 0.000 claims description 305
- 238000003860 storage Methods 0.000 claims description 34
- 239000000872 buffer Substances 0.000 claims description 28
- 238000004108 freeze drying Methods 0.000 claims description 25
- 241000588724 Escherichia coli Species 0.000 claims description 20
- 230000002528 anti-freeze Effects 0.000 claims description 15
- 241000588722 Escherichia Species 0.000 claims description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 11
- 229930006000 Sucrose Natural products 0.000 claims description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 11
- 239000005720 sucrose Substances 0.000 claims description 11
- 238000007710 freezing Methods 0.000 claims description 10
- 230000008014 freezing Effects 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 8
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 8
- 239000013598 vector Substances 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- 241000588748 Klebsiella Species 0.000 claims description 3
- 241000589516 Pseudomonas Species 0.000 claims description 3
- 101100072644 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) INO2 gene Proteins 0.000 claims description 3
- 101100454372 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LCB2 gene Proteins 0.000 claims description 3
- 101100489624 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) RTS1 gene Proteins 0.000 claims description 3
- 241000607142 Salmonella Species 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000000600 sorbitol Substances 0.000 claims description 2
- 241000220479 Acacia Species 0.000 claims 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 abstract description 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 238000009877 rendering Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 22
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- 230000003287 optical effect Effects 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 229920001451 polypropylene glycol Polymers 0.000 description 10
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 9
- 239000006285 cell suspension Substances 0.000 description 9
- 239000008188 pellet Substances 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 8
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 238000011534 incubation Methods 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 235000011056 potassium acetate Nutrition 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- 239000013599 cloning vector Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 244000144725 Amygdalus communis Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 108091029865 Exogenous DNA Proteins 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 239000007798 antifreeze agent Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- OSQPUMRCKZAIOZ-UHFFFAOYSA-N carbon dioxide;ethanol Chemical compound CCO.O=C=O OSQPUMRCKZAIOZ-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000002577 cryoprotective agent Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000002274 desiccant Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- FGDZQCVHDSGLHJ-UHFFFAOYSA-M rubidium chloride Chemical compound [Cl-].[Rb+] FGDZQCVHDSGLHJ-UHFFFAOYSA-M 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 229910021581 Cobalt(III) chloride Inorganic materials 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 241001302160 Escherichia coli str. K-12 substr. DH10B Species 0.000 description 1
- 241000488157 Escherichia sp. Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 102000020897 Formins Human genes 0.000 description 1
- 108091022623 Formins Proteins 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 241000588754 Klebsiella sp. Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000607149 Salmonella sp. Species 0.000 description 1
- 241000607758 Shigella sp. Species 0.000 description 1
- 241001147693 Staphylococcus sp. Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000194019 Streptococcus mutans Species 0.000 description 1
- 241000193998 Streptococcus pneumoniae Species 0.000 description 1
- 241000194022 Streptococcus sp. Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241001454747 Streptomyces aureus Species 0.000 description 1
- 241000187180 Streptomyces sp. Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 150000001719 carbohydrate derivatives Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 235000010299 hexamethylene tetramine Nutrition 0.000 description 1
- 239000004312 hexamethylene tetramine Substances 0.000 description 1
- VKYKSIONXSXAKP-UHFFFAOYSA-N hexamethylenetetramine Chemical compound C1N(C2)CN3CN1CN2C3 VKYKSIONXSXAKP-UHFFFAOYSA-N 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- -1 phagemids Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000000859 sublimation Methods 0.000 description 1
- 230000008022 sublimation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3972097P | 1997-02-12 | 1997-02-12 | |
| US39720P | 1997-02-12 | ||
| PCT/US1998/002880 WO1998035018A1 (en) | 1997-02-12 | 1998-02-11 | Methods for lyophilizing competent cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69829976D1 DE69829976D1 (de) | 2005-06-02 |
| DE69829976T2 true DE69829976T2 (de) | 2006-04-27 |
Family
ID=21907025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69829976T Expired - Fee Related DE69829976T2 (de) | 1997-02-12 | 1998-02-11 | Verfahren zur herstellung von zellen |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US6960464B2 (enExample) |
| EP (1) | EP1005529B1 (enExample) |
| JP (1) | JP2001512310A (enExample) |
| AT (1) | ATE294229T1 (enExample) |
| DE (1) | DE69829976T2 (enExample) |
| DK (1) | DK1005529T3 (enExample) |
| ES (1) | ES2241120T3 (enExample) |
| WO (1) | WO1998035018A1 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1005529B1 (en) * | 1997-02-12 | 2005-04-27 | Invitrogen Corporation | Methods for lyophilizing competent cells |
| US6709852B1 (en) * | 1999-06-22 | 2004-03-23 | Invitrogen Corporation | Rapid growing microorganisms for biotechnology applications |
| US20020081565A1 (en) * | 2000-10-30 | 2002-06-27 | Sigma-Aldrich Co. | Process for producing freeze dried competent cells and use thereof in cloning |
| WO2002061111A2 (en) * | 2000-12-15 | 2002-08-08 | Stratagene | Room temperature stable competent cells |
| US20040110267A1 (en) * | 2000-12-15 | 2004-06-10 | Stratagene | Room temperature stable competent cells |
| WO2004065568A2 (en) * | 2003-01-23 | 2004-08-05 | Invitrogen Corporation | Rapid growing microorganisms for biotechnology applications |
| WO2004085613A2 (en) * | 2003-03-19 | 2004-10-07 | Stratagene | Electrocompetent host cells |
| TWI414602B (zh) | 2010-12-14 | 2013-11-11 | Ind Tech Res Inst | 新穎之大腸桿菌與其用途以及製備勝任細胞的方法 |
| CN103387946A (zh) * | 2012-05-11 | 2013-11-13 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 一种感受态宿主细胞的制备方法以及一种快速高效将外源物质转入宿主细胞的方法及其应用 |
| CN105209601A (zh) * | 2012-10-16 | 2015-12-30 | 基因桥有限责任公司 | 电感受态细胞及其制备 |
| KR102122632B1 (ko) | 2013-08-05 | 2020-06-16 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
| US9752829B2 (en) * | 2014-01-07 | 2017-09-05 | Sudhir R. Brahmbhatt | Liquid nitrogen (LIN) integrated lyophilization system for minimizing a carbon footprint |
| US9963672B2 (en) | 2014-07-21 | 2018-05-08 | New England Biolabs, Inc. | Compositions and methods for extending storage time of competent cells at -20° C |
| CA3253836A1 (en) | 2015-02-04 | 2025-12-01 | Twist Bioscience Corporation | Compositions and methods for synthetic gene assembly |
| WO2016126882A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| WO2016172377A1 (en) | 2015-04-21 | 2016-10-27 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| EP3350314A4 (en) | 2015-09-18 | 2019-02-06 | Twist Bioscience Corporation | OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| CN108603307A (zh) | 2015-12-01 | 2018-09-28 | 特韦斯特生物科学公司 | 功能化表面及其制备 |
| GB2568444A (en) | 2016-08-22 | 2019-05-15 | Twist Bioscience Corp | De novo synthesized nucleic acid libraries |
| US10417457B2 (en) | 2016-09-21 | 2019-09-17 | Twist Bioscience Corporation | Nucleic acid based data storage |
| US10463065B2 (en) * | 2016-12-01 | 2019-11-05 | Council Of Scientific And Industrial Research | Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni |
| CN110366613A (zh) | 2016-12-16 | 2019-10-22 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
| SG11201907713WA (en) | 2017-02-22 | 2019-09-27 | Twist Bioscience Corp | Nucleic acid based data storage |
| CN110913865A (zh) | 2017-03-15 | 2020-03-24 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| KR102784398B1 (ko) | 2017-06-12 | 2025-03-19 | 트위스트 바이오사이언스 코포레이션 | 심리스 핵산 어셈블리를 위한 방법 |
| JP2020536504A (ja) | 2017-09-11 | 2020-12-17 | ツイスト バイオサイエンス コーポレーション | Gpcr結合タンパク質およびその合成 |
| KR102889470B1 (ko) | 2017-10-20 | 2025-11-21 | 트위스트 바이오사이언스 코포레이션 | 폴리뉴클레오타이드 합성을 위한 가열된 나노웰 |
| EP3728581B1 (en) * | 2017-12-22 | 2024-10-09 | Bio-Rad Laboratories, Inc. | Controlling phenotype of organisms with crispr/cas gene targeting |
| WO2019136175A1 (en) | 2018-01-04 | 2019-07-11 | Twist Bioscience Corporation | Dna-based digital information storage |
| CA3100739A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| CN119708086A (zh) | 2018-12-26 | 2025-03-28 | 特韦斯特生物科学公司 | 高度准确的从头多核苷酸合成 |
| EP3930753A4 (en) | 2019-02-26 | 2023-03-29 | Twist Bioscience Corporation | VARIANT NUCLEIC ACID LIBRARIES FOR GLP1 RECEPTOR |
| AU2020227802A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| CN114729342A (zh) | 2019-06-21 | 2022-07-08 | 特韦斯特生物科学公司 | 基于条形码的核酸序列装配 |
| WO2021061829A1 (en) | 2019-09-23 | 2021-04-01 | Twist Bioscience Corporation | Variant nucleic acid libraries for crth2 |
| AU2020355027A1 (en) | 2019-09-23 | 2022-04-21 | Twist Bioscience Corporation | Antibodies that bind CD3 Epsilon |
| CN113817609A (zh) * | 2021-11-01 | 2021-12-21 | 苏州科正环保科技有限公司 | 基因工程菌的冻干保藏方法 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3843453A (en) | 1970-11-27 | 1974-10-22 | Miles Lab | Process and composition for use in microbiological quality assurance |
| US4038143A (en) | 1973-10-29 | 1977-07-26 | The Regents Of The University Of Michigan | Test kit for the genetic detection of microorganisms |
| DE3128411A1 (de) | 1980-07-18 | 1983-09-15 | Unisearch Ltd., 2033 Kensington, New South Wales | Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalanin |
| US4446230A (en) | 1981-10-30 | 1984-05-01 | Temple University Of The Commonwealth System Of Higher Education | Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae |
| US4520019A (en) | 1982-04-29 | 1985-05-28 | Ribi Immunochem Research, Inc. | Stable composition and preparation thereof |
| JPS60258125A (ja) | 1984-06-06 | 1985-12-20 | Hayashibara Biochem Lab Inc | 蛋白性生理活性物質を含有する水溶性乾燥物 |
| DE3515586A1 (de) | 1985-04-30 | 1986-11-06 | Boehringer Mannheim Gmbh, 6800 Mannheim | Stabilisiertes sarcosinoxidase-praeparat |
| JPH0779694B2 (ja) | 1985-07-09 | 1995-08-30 | カドラント バイオリソ−シズ リミテツド | 蛋白質および同類品の保護 |
| US4981797A (en) | 1985-08-08 | 1991-01-01 | Life Technologies, Inc. | Process of producing highly transformable cells and cells produced thereby |
| US4851348A (en) | 1986-07-16 | 1989-07-25 | Cold Spring Harbor Laboratory | Biologically pure escherichia coli cell line which is a deoR- mutant and which is more transformation efficient with foreign plasmids than deoR+ escherichia coli cell lines, processes for obtaining these cell lines, methods of use |
| US5292507A (en) | 1986-08-01 | 1994-03-08 | Imperial Oil Limited | Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds |
| FR2625221B1 (fr) | 1987-12-24 | 1990-06-15 | Sanofi Sa | Protoplastes stabilises et procede pour leur obtention |
| US4808404A (en) | 1988-01-11 | 1989-02-28 | A. H. Robins Company, Inc. | Live vaccine for coccidiosis utilizing coccidial sporozoites |
| US5043261A (en) | 1989-04-10 | 1991-08-27 | Cryopharm Corporation | Lyophilized and reconstituted red blood cell and hemosome compositions |
| US5153004A (en) | 1989-06-02 | 1992-10-06 | Cryopharm Corporation | Freezing and thawing of erythrocytes |
| US5178884A (en) | 1988-05-18 | 1993-01-12 | Cryopharm Corporation | Lyophilized and reconstituted red blood cell compositions |
| US5213814A (en) | 1989-04-10 | 1993-05-25 | Cryopharm Corporation | Lyophilized and reconstituted blood platelet compositions |
| US5958670A (en) | 1988-05-18 | 1999-09-28 | Cobe Laboratories, Inc. | Method of freezing cells and cell-like materials |
| US5045446A (en) | 1988-08-26 | 1991-09-03 | Cryopharm Corporation | Lyophilization of cells |
| US5059518A (en) | 1988-10-20 | 1991-10-22 | Coulter Corporation | Stabilized lyophilized mammalian cells and method of making same |
| GB8903593D0 (en) | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| US5149656A (en) | 1990-05-03 | 1992-09-22 | University Of Florida Research Foundation, Inc. | Microbiological assay pad and kit for selective detection of toxicants |
| US5200399A (en) * | 1990-09-14 | 1993-04-06 | Boyce Thompson Institute For Plant Research, Inc. | Method of protecting biological materials from destructive reactions in the dry state |
| US5733774A (en) * | 1995-02-02 | 1998-03-31 | Ecoscience Corporation | Method and composition for producing stable bacteria and bacterial formulations |
| US6274369B1 (en) | 1996-02-02 | 2001-08-14 | Invitrogen Corporation | Method capable of increasing competency of bacterial cell transformation |
| WO1997036613A1 (en) | 1996-03-29 | 1997-10-09 | Life Technologies, Inc. | Method for increasing viability and transformation efficiency of bacteria during storage at low temperatures |
| EP1005529B1 (en) | 1997-02-12 | 2005-04-27 | Invitrogen Corporation | Methods for lyophilizing competent cells |
| US6383810B2 (en) | 1997-02-14 | 2002-05-07 | Invitrogen Corporation | Dry powder cells and cell culture reagents and methods of production thereof |
| US6610531B1 (en) * | 1998-09-24 | 2003-08-26 | The United States Of America As Represented By The Secretary Of The Navy | Viable dried bacteria produced by drying in the presence of trehalose and divalent cation |
| US20030044965A1 (en) * | 1998-09-24 | 2003-03-06 | Alfred Mateczun | Long term preservation and storage of viable dried bacteria |
| US20020081565A1 (en) * | 2000-10-30 | 2002-06-27 | Sigma-Aldrich Co. | Process for producing freeze dried competent cells and use thereof in cloning |
| US6872357B1 (en) * | 2000-11-22 | 2005-03-29 | Quadrant Drug Delivery Limited | Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying |
| US20040110267A1 (en) * | 2000-12-15 | 2004-06-10 | Stratagene | Room temperature stable competent cells |
| AU2002248178A1 (en) * | 2000-12-15 | 2002-08-12 | Stratagene | Mutant cells with enhanced resistance to desiccation |
| WO2002061111A2 (en) * | 2000-12-15 | 2002-08-08 | Stratagene | Room temperature stable competent cells |
| AU2002305461A1 (en) * | 2001-05-07 | 2002-11-18 | Universal Preservation Technologies, Inc. | Preservation of competent cells at ambient temperatures |
-
1998
- 1998-02-11 EP EP98906440A patent/EP1005529B1/en not_active Expired - Lifetime
- 1998-02-11 AT AT98906440T patent/ATE294229T1/de not_active IP Right Cessation
- 1998-02-11 DK DK98906440T patent/DK1005529T3/da active
- 1998-02-11 DE DE69829976T patent/DE69829976T2/de not_active Expired - Fee Related
- 1998-02-11 ES ES98906440T patent/ES2241120T3/es not_active Expired - Lifetime
- 1998-02-11 JP JP53508298A patent/JP2001512310A/ja not_active Ceased
- 1998-02-11 WO PCT/US1998/002880 patent/WO1998035018A1/en not_active Ceased
-
2002
- 2002-04-11 US US10/120,163 patent/US6960464B2/en not_active Expired - Lifetime
-
2005
- 2005-08-31 US US11/214,800 patent/US7648832B2/en not_active Expired - Fee Related
-
2009
- 2009-12-03 US US12/630,542 patent/US20100081171A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001512310A (ja) | 2001-08-21 |
| EP1005529A1 (en) | 2000-06-07 |
| US6960464B2 (en) | 2005-11-01 |
| ATE294229T1 (de) | 2005-05-15 |
| US20020164771A1 (en) | 2002-11-07 |
| WO1998035018A1 (en) | 1998-08-13 |
| ES2241120T3 (es) | 2005-10-16 |
| DK1005529T3 (da) | 2005-06-13 |
| US20060014255A1 (en) | 2006-01-19 |
| EP1005529A4 (en) | 2002-06-12 |
| DE69829976D1 (de) | 2005-06-02 |
| US7648832B2 (en) | 2010-01-19 |
| US20100081171A1 (en) | 2010-04-01 |
| EP1005529B1 (en) | 2005-04-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69829976T2 (de) | Verfahren zur herstellung von zellen | |
| DE69129253T2 (de) | Verfahren und Vorrichtung zur Kryopräparation, trockene Stabilisierung und Rehydratisierung von Suspensionen von Zellen | |
| DE3685968T2 (de) | Zellulare einkapselung biologischer pestizide. | |
| DE3789401T2 (de) | Biologische Kryoprotektion. | |
| US6127177A (en) | Controlled reversible poration for preservation of biological materials | |
| WO1998035018A9 (en) | Methods for lyophilizing competent cells | |
| Pye et al. | Neoaplectana carpocapsae: nematode accumulations on chemical and bacterial gradients | |
| DE68908206T2 (de) | Gegen Coleoptera wirksamer Bacillus thuringiensis isolate. | |
| Kiviranta et al. | Toxicity of the blue-green alga Oscillatoria agardhii to the mosquito Aedes aegypti and the shrimp Artemia salina | |
| US6610531B1 (en) | Viable dried bacteria produced by drying in the presence of trehalose and divalent cation | |
| PL133923B1 (en) | Method of obtaining freeze dried hepatitis vaccine for ducks | |
| CH629533A5 (de) | Verfahren zur gewinnung von insulin mittels genetisch umgebildeter funguszellen. | |
| DE3685673T2 (de) | Verfahren zur aufbewahrung von saeure produzierenden bakterien und demgemaess hergestellte zusammensetzungen. | |
| Criste et al. | Research concerning use of long-term preservation techniques for microorganisms | |
| Warren | Bacterial ice nucleation: molecular biology and applications | |
| DE68916806T2 (de) | Neue Bacillus Thuringiensis-Stämme. | |
| Johny et al. | Parasitization potential of two cephaline gregarines, Leidyana subramanii Pushkala and Muralirangan and Retractocephalus dhawanii sp. n. on the tobacco grasshopper, Atractomorpha crenulata (Fab.) | |
| Holm-Hansen | Viability of lyophilized algae | |
| DE102021116694B4 (de) | Wässrige Lösung zur Zellkonservierung | |
| US20040185425A1 (en) | Method for dry-preserving multicellular organism tissue at ordinary temperatures | |
| CN105028387A (zh) | 昆虫线虫保湿剂及昆虫线虫保存方法 | |
| EP1302532B1 (de) | Verfahren zur Erhöhung der Wachstumsrate von Zellen in statischen Flüssigmedien | |
| Papageorgiou et al. | Immobilization of photosynthetically active cyanobacteria in glutaraldehyde-crosslinked albumin matrix | |
| US20230225310A1 (en) | Composition for cryoprotection including dna nanostructure, and method for using same | |
| Miskimen | Nonaseptic laboratory rearing of the sugarcane borer, Diatraea saccharalis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |