ES2241120T3 - Metodos para liofilizar celulas competentes. - Google Patents
Metodos para liofilizar celulas competentes.Info
- Publication number
- ES2241120T3 ES2241120T3 ES98906440T ES98906440T ES2241120T3 ES 2241120 T3 ES2241120 T3 ES 2241120T3 ES 98906440 T ES98906440 T ES 98906440T ES 98906440 T ES98906440 T ES 98906440T ES 2241120 T3 ES2241120 T3 ES 2241120T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- competent
- transformation efficiency
- lyophilized
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 94
- 210000004027 cell Anatomy 0.000 claims abstract description 304
- 230000009466 transformation Effects 0.000 claims abstract description 58
- 230000012010 growth Effects 0.000 claims abstract description 13
- 210000001236 prokaryotic cell Anatomy 0.000 claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 claims abstract description 5
- 108020004414 DNA Proteins 0.000 claims description 51
- 238000003860 storage Methods 0.000 claims description 36
- 239000000872 buffer Substances 0.000 claims description 29
- 102000053602 DNA Human genes 0.000 claims description 28
- 238000004108 freeze drying Methods 0.000 claims description 25
- 108090000623 proteins and genes Proteins 0.000 claims description 25
- 241000588724 Escherichia coli Species 0.000 claims description 22
- 239000002577 cryoprotective agent Substances 0.000 claims description 20
- 102000004169 proteins and genes Human genes 0.000 claims description 19
- 241000588722 Escherichia Species 0.000 claims description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 11
- 229930006000 Sucrose Natural products 0.000 claims description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 11
- 239000005720 sucrose Substances 0.000 claims description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 6
- 239000013598 vector Substances 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 150000001720 carbohydrates Chemical group 0.000 claims description 4
- 241000588748 Klebsiella Species 0.000 claims description 3
- 241000589516 Pseudomonas Species 0.000 claims description 3
- 101100072644 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) INO2 gene Proteins 0.000 claims description 3
- 101100454372 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LCB2 gene Proteins 0.000 claims description 3
- 101100489624 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) RTS1 gene Proteins 0.000 claims description 3
- 241000607142 Salmonella Species 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- 229920000084 Gum arabic Polymers 0.000 claims description 2
- 239000000205 acacia gum Substances 0.000 claims description 2
- 235000010489 acacia gum Nutrition 0.000 claims description 2
- 239000000600 sorbitol Substances 0.000 claims description 2
- 241000978776 Senegalia senegal Species 0.000 claims 1
- 125000000647 trehalose group Chemical group 0.000 claims 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- 239000002609 medium Substances 0.000 description 18
- 230000003287 optical effect Effects 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 229920001451 polypropylene glycol Polymers 0.000 description 10
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 9
- 239000006285 cell suspension Substances 0.000 description 8
- 238000007710 freezing Methods 0.000 description 8
- 230000008014 freezing Effects 0.000 description 8
- 239000008188 pellet Substances 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 8
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 235000011089 carbon dioxide Nutrition 0.000 description 4
- 230000003833 cell viability Effects 0.000 description 4
- 239000013599 cloning vector Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 235000011056 potassium acetate Nutrition 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 230000001131 transforming effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 244000144725 Amygdalus communis Species 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 108091029865 Exogenous DNA Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000002274 desiccant Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- -1 phagemids Substances 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- FGDZQCVHDSGLHJ-UHFFFAOYSA-M rubidium chloride Chemical compound [Cl-].[Rb+] FGDZQCVHDSGLHJ-UHFFFAOYSA-M 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 241000488157 Escherichia sp. Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 241000588754 Klebsiella sp. Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000607149 Salmonella sp. Species 0.000 description 1
- 241000607758 Shigella sp. Species 0.000 description 1
- 241001147693 Staphylococcus sp. Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000194019 Streptococcus mutans Species 0.000 description 1
- 241000193998 Streptococcus pneumoniae Species 0.000 description 1
- 241000194022 Streptococcus sp. Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241001454747 Streptomyces aureus Species 0.000 description 1
- 241000187180 Streptomyces sp. Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 150000001719 carbohydrate derivatives Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002270 exclusion chromatography Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229940055835 triptone Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000002061 vacuum sublimation Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3972097P | 1997-02-12 | 1997-02-12 | |
| US39720P | 1997-02-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2241120T3 true ES2241120T3 (es) | 2005-10-16 |
Family
ID=21907025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES98906440T Expired - Lifetime ES2241120T3 (es) | 1997-02-12 | 1998-02-11 | Metodos para liofilizar celulas competentes. |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US6960464B2 (enExample) |
| EP (1) | EP1005529B1 (enExample) |
| JP (1) | JP2001512310A (enExample) |
| AT (1) | ATE294229T1 (enExample) |
| DE (1) | DE69829976T2 (enExample) |
| DK (1) | DK1005529T3 (enExample) |
| ES (1) | ES2241120T3 (enExample) |
| WO (1) | WO1998035018A1 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69829976T2 (de) * | 1997-02-12 | 2006-04-27 | Invitrogen Corp., Carlsbad | Verfahren zur herstellung von zellen |
| US6709852B1 (en) * | 1999-06-22 | 2004-03-23 | Invitrogen Corporation | Rapid growing microorganisms for biotechnology applications |
| US20020081565A1 (en) * | 2000-10-30 | 2002-06-27 | Sigma-Aldrich Co. | Process for producing freeze dried competent cells and use thereof in cloning |
| AU2002248192A1 (en) * | 2000-12-15 | 2002-08-12 | Stratagene | Room temperature stable competent cells |
| US20040110267A1 (en) * | 2000-12-15 | 2004-06-10 | Stratagene | Room temperature stable competent cells |
| US20040214306A1 (en) * | 2003-01-23 | 2004-10-28 | Bloom Fredric R. | Rapid growing microorganisms for biotechnology applications |
| US20040209362A1 (en) * | 2003-03-19 | 2004-10-21 | Stratagene | Electrocompetent host cells |
| TWI414602B (zh) | 2010-12-14 | 2013-11-11 | Ind Tech Res Inst | 新穎之大腸桿菌與其用途以及製備勝任細胞的方法 |
| CN103387946A (zh) * | 2012-05-11 | 2013-11-13 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 一种感受态宿主细胞的制备方法以及一种快速高效将外源物质转入宿主细胞的方法及其应用 |
| GB2520903A (en) | 2012-10-16 | 2015-06-03 | Gene Bridges Gmbh | Electrocompetent cells and preparation thereof |
| TWI707038B (zh) | 2013-08-05 | 2020-10-11 | 美商扭轉生物科技有限公司 | 重新合成之基因庫 |
| US9752829B2 (en) * | 2014-01-07 | 2017-09-05 | Sudhir R. Brahmbhatt | Liquid nitrogen (LIN) integrated lyophilization system for minimizing a carbon footprint |
| WO2016014307A1 (en) * | 2014-07-21 | 2016-01-28 | New England Biolabs, Inc. | Compositions and methods for extending storage time of competent cells at -20 c |
| CA2975855C (en) | 2015-02-04 | 2025-09-23 | Twist Bioscience Corporation | SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS |
| WO2016126882A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| HK1258869A1 (zh) | 2015-09-18 | 2019-11-22 | Twist Bioscience Corporation | 寡核酸变体文库及其合成 |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| US9895673B2 (en) | 2015-12-01 | 2018-02-20 | Twist Bioscience Corporation | Functionalized surfaces and preparation thereof |
| SG11201901563UA (en) | 2016-08-22 | 2019-03-28 | Twist Bioscience Corp | De novo synthesized nucleic acid libraries |
| US10417457B2 (en) | 2016-09-21 | 2019-09-17 | Twist Bioscience Corporation | Nucleic acid based data storage |
| US10463065B2 (en) * | 2016-12-01 | 2019-11-05 | Council Of Scientific And Industrial Research | Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni |
| AU2017378492B2 (en) | 2016-12-16 | 2022-06-16 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| US11550939B2 (en) | 2017-02-22 | 2023-01-10 | Twist Bioscience Corporation | Nucleic acid based data storage using enzymatic bioencryption |
| EP3595674A4 (en) | 2017-03-15 | 2020-12-16 | Twist Bioscience Corporation | BANKS OF VARIANTS OF IMMUNOLOGICAL SYNAPSE AND THEIR SYNTHESIS |
| IL271205B2 (en) | 2017-06-12 | 2025-02-01 | Twist Bioscience Corp | Methods for assembling continuous nucleic acids |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| GB2581620A (en) | 2017-09-11 | 2020-08-26 | Twist Bioscience Corp | GPCR binding proteins and synthesis thereof |
| CA3079613A1 (en) | 2017-10-20 | 2019-04-25 | Twist Bioscience Corporation | Heated nanowells for polynucleotide synthesis |
| WO2019126562A1 (en) * | 2017-12-22 | 2019-06-27 | Bio-Rad Laboratories, Inc. | Controlling phenotype of organisms with crispr/cas gene targeting |
| GB2585506A (en) | 2018-01-04 | 2021-01-13 | Twist Bioscience Corp | DNA-based digital information storage |
| CA3100739A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| EP3902816A4 (en) | 2018-12-26 | 2022-09-14 | Twist Bioscience Corporation | HIGHLY ACCURATE POLYNUCLEOTIDE DE NOVO SYNTHESIS |
| AU2020227802A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| JP2022521551A (ja) | 2019-02-26 | 2022-04-08 | ツイスト バイオサイエンス コーポレーション | Ｇｌｐ１受容体の変異体核酸ライブラリ |
| AU2020298294A1 (en) | 2019-06-21 | 2022-02-17 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
| US12091777B2 (en) | 2019-09-23 | 2024-09-17 | Twist Bioscience Corporation | Variant nucleic acid libraries for CRTH2 |
| EP4034564A4 (en) | 2019-09-23 | 2023-12-13 | Twist Bioscience Corporation | VARIANT NUCLEIC ACID LIBRARIES FOR SINGLE-DOMAIN ANTIBODIES |
| CN113817609A (zh) * | 2021-11-01 | 2021-12-21 | 苏州科正环保科技有限公司 | 基因工程菌的冻干保藏方法 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3843453A (en) | 1970-11-27 | 1974-10-22 | Miles Lab | Process and composition for use in microbiological quality assurance |
| US4038143A (en) | 1973-10-29 | 1977-07-26 | The Regents Of The University Of Michigan | Test kit for the genetic detection of microorganisms |
| JPS57170184A (en) | 1980-07-18 | 1982-10-20 | Unisearch Ltd | Mutant of e. coli and production of phenylalanine |
| US4446230A (en) | 1981-10-30 | 1984-05-01 | Temple University Of The Commonwealth System Of Higher Education | Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae |
| US4520019A (en) | 1982-04-29 | 1985-05-28 | Ribi Immunochem Research, Inc. | Stable composition and preparation thereof |
| JPS60258125A (ja) | 1984-06-06 | 1985-12-20 | Hayashibara Biochem Lab Inc | 蛋白性生理活性物質を含有する水溶性乾燥物 |
| DE3515586A1 (de) | 1985-04-30 | 1986-11-06 | Boehringer Mannheim Gmbh, 6800 Mannheim | Stabilisiertes sarcosinoxidase-praeparat |
| EP0229810B1 (en) | 1985-07-09 | 1991-10-16 | Quadrant Bioresources Limited | Protection of proteins and the like |
| US4981797A (en) | 1985-08-08 | 1991-01-01 | Life Technologies, Inc. | Process of producing highly transformable cells and cells produced thereby |
| US4851348A (en) | 1986-07-16 | 1989-07-25 | Cold Spring Harbor Laboratory | Biologically pure escherichia coli cell line which is a deoR- mutant and which is more transformation efficient with foreign plasmids than deoR+ escherichia coli cell lines, processes for obtaining these cell lines, methods of use |
| US5292507A (en) | 1986-08-01 | 1994-03-08 | Imperial Oil Limited | Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds |
| FR2625221B1 (fr) | 1987-12-24 | 1990-06-15 | Sanofi Sa | Protoplastes stabilises et procede pour leur obtention |
| US4808404A (en) | 1988-01-11 | 1989-02-28 | A. H. Robins Company, Inc. | Live vaccine for coccidiosis utilizing coccidial sporozoites |
| US5043261A (en) | 1989-04-10 | 1991-08-27 | Cryopharm Corporation | Lyophilized and reconstituted red blood cell and hemosome compositions |
| US5958670A (en) | 1988-05-18 | 1999-09-28 | Cobe Laboratories, Inc. | Method of freezing cells and cell-like materials |
| US5178884A (en) | 1988-05-18 | 1993-01-12 | Cryopharm Corporation | Lyophilized and reconstituted red blood cell compositions |
| US5153004A (en) | 1989-06-02 | 1992-10-06 | Cryopharm Corporation | Freezing and thawing of erythrocytes |
| US5213814A (en) | 1989-04-10 | 1993-05-25 | Cryopharm Corporation | Lyophilized and reconstituted blood platelet compositions |
| US5045446A (en) | 1988-08-26 | 1991-09-03 | Cryopharm Corporation | Lyophilization of cells |
| US5059518A (en) | 1988-10-20 | 1991-10-22 | Coulter Corporation | Stabilized lyophilized mammalian cells and method of making same |
| GB8903593D0 (en) | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| US5149656A (en) | 1990-05-03 | 1992-09-22 | University Of Florida Research Foundation, Inc. | Microbiological assay pad and kit for selective detection of toxicants |
| US5200399A (en) | 1990-09-14 | 1993-04-06 | Boyce Thompson Institute For Plant Research, Inc. | Method of protecting biological materials from destructive reactions in the dry state |
| US5733774A (en) | 1995-02-02 | 1998-03-31 | Ecoscience Corporation | Method and composition for producing stable bacteria and bacterial formulations |
| US6274369B1 (en) | 1996-02-02 | 2001-08-14 | Invitrogen Corporation | Method capable of increasing competency of bacterial cell transformation |
| AU2590997A (en) | 1996-03-29 | 1997-10-22 | Life Technologies, Inc. | Method for increasing viability and transformation efficiency of bacteria during storage at low temperatures |
| DE69829976T2 (de) | 1997-02-12 | 2006-04-27 | Invitrogen Corp., Carlsbad | Verfahren zur herstellung von zellen |
| US6383810B2 (en) | 1997-02-14 | 2002-05-07 | Invitrogen Corporation | Dry powder cells and cell culture reagents and methods of production thereof |
| US6610531B1 (en) | 1998-09-24 | 2003-08-26 | The United States Of America As Represented By The Secretary Of The Navy | Viable dried bacteria produced by drying in the presence of trehalose and divalent cation |
| US20030044965A1 (en) | 1998-09-24 | 2003-03-06 | Alfred Mateczun | Long term preservation and storage of viable dried bacteria |
| US20020081565A1 (en) | 2000-10-30 | 2002-06-27 | Sigma-Aldrich Co. | Process for producing freeze dried competent cells and use thereof in cloning |
| US6872357B1 (en) | 2000-11-22 | 2005-03-29 | Quadrant Drug Delivery Limited | Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying |
| US20040110267A1 (en) | 2000-12-15 | 2004-06-10 | Stratagene | Room temperature stable competent cells |
| AU2002248192A1 (en) | 2000-12-15 | 2002-08-12 | Stratagene | Room temperature stable competent cells |
| WO2002061035A2 (en) | 2000-12-15 | 2002-08-08 | Stratagene | Mutant cells with enhanced resistance to desiccation |
| US20050074867A1 (en) | 2001-05-07 | 2005-04-07 | Victor Bronshtein | Preservation of competent cells at ambient temperatures |
-
1998
- 1998-02-11 DE DE69829976T patent/DE69829976T2/de not_active Expired - Fee Related
- 1998-02-11 DK DK98906440T patent/DK1005529T3/da active
- 1998-02-11 WO PCT/US1998/002880 patent/WO1998035018A1/en not_active Ceased
- 1998-02-11 JP JP53508298A patent/JP2001512310A/ja not_active Ceased
- 1998-02-11 AT AT98906440T patent/ATE294229T1/de not_active IP Right Cessation
- 1998-02-11 EP EP98906440A patent/EP1005529B1/en not_active Expired - Lifetime
- 1998-02-11 ES ES98906440T patent/ES2241120T3/es not_active Expired - Lifetime
-
2002
- 2002-04-11 US US10/120,163 patent/US6960464B2/en not_active Expired - Lifetime
-
2005
- 2005-08-31 US US11/214,800 patent/US7648832B2/en not_active Expired - Fee Related
-
2009
- 2009-12-03 US US12/630,542 patent/US20100081171A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1005529A1 (en) | 2000-06-07 |
| EP1005529B1 (en) | 2005-04-27 |
| DE69829976D1 (de) | 2005-06-02 |
| US7648832B2 (en) | 2010-01-19 |
| ATE294229T1 (de) | 2005-05-15 |
| US6960464B2 (en) | 2005-11-01 |
| DE69829976T2 (de) | 2006-04-27 |
| US20020164771A1 (en) | 2002-11-07 |
| US20060014255A1 (en) | 2006-01-19 |
| EP1005529A4 (en) | 2002-06-12 |
| DK1005529T3 (da) | 2005-06-13 |
| WO1998035018A1 (en) | 1998-08-13 |
| US20100081171A1 (en) | 2010-04-01 |
| JP2001512310A (ja) | 2001-08-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2241120T3 (es) | Metodos para liofilizar celulas competentes. | |
| WO1998035018A9 (en) | Methods for lyophilizing competent cells | |
| ES2273854T3 (es) | Medio de preservacion y almacenamiento para materiales biologicos. | |
| AU650045B2 (en) | Method and apparatus for cryopreparation dry stabilization and rehydration of biological suspensions | |
| Uzunova-Doneva et al. | Anabiosis and conservation of microorganisms | |
| Jang et al. | Cryopreservation and its clinical applications | |
| US6610531B1 (en) | Viable dried bacteria produced by drying in the presence of trehalose and divalent cation | |
| Criste et al. | Research concerning use of long-term preservation techniques for microorganisms | |
| Anderson et al. | The effects of streptomycin, desiccation, and UV radiation on ice nucleation by Pseudomonas viridiflava | |
| Morrison et al. | Flagellar changes in Escherichia coli induced by temperature of the environment | |
| US10611996B2 (en) | Preservation and storage of biological specimens | |
| Acker et al. | Engineering desiccation tolerance in mammalian cells: tools and techniques | |
| WO1991012718A1 (en) | Enhanced cryopreservation with thermal hysteresis peptide | |
| Mortain‐Bertrand et al. | A METHOD FOR THE CRYOCONSERVATION OF DUNALIELLA SALINA (CHLOROPHYCEAE): EFFECT OF GLYCEROL AND COLD ADAPTATION 1 | |
| MORIGUCHI et al. | Freeze-preservation of dormant pear shoot apices | |
| Adams | The preservation of inocula | |
| US20030044965A1 (en) | Long term preservation and storage of viable dried bacteria | |
| Sidyakina et al. | Viability and genetic stability of the bacterium Escherichia coli HB101 with the recombinant plasmid during preservation by various methods | |
| Holm-Hansen | Viability of lyophilized algae | |
| Thomas et al. | Cryogenic light microscopy and the development of long-term cryopreservation techniques for fungi | |
| SU1671682A1 (ru) | Способ хранени микроорганизмов | |
| KR100390385B1 (ko) | 미생물의 동결건조방법 | |
| RU2822476C1 (ru) | Способ длительного сохранения (консервирования) облигатно анаэробных бактерий высушиванием из замороженного состояния | |
| JP4490038B2 (ja) | コンピテント細胞または形質転換細胞の作製法 | |
| RU2036234C1 (ru) | Консервант для хранения перевиваемых клеток |