DE69735382T2 - Kationische reagenzien zür transfektion - Google Patents
Kationische reagenzien zür transfektion Download PDFInfo
- Publication number
- DE69735382T2 DE69735382T2 DE69735382T DE69735382T DE69735382T2 DE 69735382 T2 DE69735382 T2 DE 69735382T2 DE 69735382 T DE69735382 T DE 69735382T DE 69735382 T DE69735382 T DE 69735382T DE 69735382 T2 DE69735382 T2 DE 69735382T2
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- Germany
- Prior art keywords
- alkyl
- straight
- chain
- branched
- alkynyl
- Prior art date
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- Expired - Lifetime
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
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- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
- A61K47/544—Phospholipids
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
- A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers
- A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
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- Zoology (AREA)
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- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3031596P | 1996-11-04 | 1996-11-04 | |
| US30315P | 1996-11-04 | ||
| PCT/EP1997/006035 WO1998019709A2 (en) | 1996-11-04 | 1997-11-03 | Cationic reagents for transfection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69735382D1 DE69735382D1 (de) | 2006-04-27 |
| DE69735382T2 true DE69735382T2 (de) | 2006-11-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69735382T Expired - Lifetime DE69735382T2 (de) | 1996-11-04 | 1997-11-03 | Kationische reagenzien zür transfektion |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6733777B2 (https=) |
| EP (1) | EP1003556B1 (https=) |
| JP (1) | JP4175667B2 (https=) |
| AU (1) | AU738384B2 (https=) |
| CA (1) | CA2270396C (https=) |
| DE (1) | DE69735382T2 (https=) |
| WO (1) | WO1998019709A2 (https=) |
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| US5674908A (en) * | 1993-12-20 | 1997-10-07 | Life Technologies, Inc. | Highly packed polycationic ammonium, sulfonium and phosphonium lipids |
| US6989434B1 (en) | 1994-02-11 | 2006-01-24 | Invitrogen Corporation | Reagents for intracellular delivery of macromolecules |
| DE69735382T2 (de) * | 1996-11-04 | 2006-11-30 | Qiagen Gmbh | Kationische reagenzien zür transfektion |
| DK1129064T3 (da) | 1998-11-12 | 2008-04-28 | Invitrogen Corp | Transfektionsreagenser |
| CA2299119C (en) * | 1999-02-23 | 2013-02-05 | Qiagen Gmbh | A method of stabilizing and/or isolating nucleic acids |
| US20030096414A1 (en) | 2001-03-27 | 2003-05-22 | Invitrogen Corporation | Culture medium for cell growth and transfection |
| US7906122B2 (en) | 2003-06-18 | 2011-03-15 | Yissum Research Development Company Of The Hebrew University Of Jersusalem | Sphingoid polyalkylamine conjugates for Hepatitis B virus vaccination |
| MXPA05013746A (es) | 2003-06-18 | 2006-06-27 | Yissum Res Dev Co | Conjugados de esfingoide-polialquilamina para vacunacion. |
| US20060211004A1 (en) | 2005-02-15 | 2006-09-21 | Ilsley Diane D | Methods and compositions for determining non-specific cytotoxicity of a transfection agent |
| US20060257858A1 (en) * | 2005-04-01 | 2006-11-16 | Invitrogen Corporation | Methods and compositions for introducing biopolymers into cells |
| EP2366787B1 (en) | 2006-01-13 | 2019-12-11 | The Government Of The U.S.A, As Represented By The Secretary, Dept. Of Health And Human Services | Codon optimized IL-15 and IL-15R-Alpha genes for expression in mammalian cells |
| CA2686735A1 (en) | 2007-05-04 | 2008-11-13 | Mdrna, Inc. | Amino acid lipids and uses thereof |
| CA2726187A1 (en) | 2008-05-30 | 2009-12-23 | Yale University | Targeted oligonucleotide compositions for modifying gene expression |
| CN102231979B (zh) | 2008-10-16 | 2014-10-29 | 玛瑞纳生物技术有限公司 | 基因沉默治疗剂的脂质体有效递送方法和组合物 |
| HUE042177T2 (hu) | 2009-12-01 | 2019-06-28 | Translate Bio Inc | Szteroidszármazék mRNS szállítására humán genetikai betegségekben |
| US20130052221A1 (en) | 2010-02-26 | 2013-02-28 | The Govt. of the U.S, as represented by The Sec. of The Dept. of Health and Human Services | Dna-protein vaccination protocols |
| WO2011120023A1 (en) | 2010-03-26 | 2011-09-29 | Marina Biotech, Inc. | Nucleic acid compounds for inhibiting survivin gene expression uses thereof |
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| US3966630A (en) * | 1972-03-24 | 1976-06-29 | Petrolite Corporation | Use of polyquaternary ammonium methylene phosphonates in chelating or scale inhibition |
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| CA2214029A1 (en) * | 1995-04-25 | 1996-10-31 | Magda Marquet | Single-vial formulations of dna/lipid complexes |
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| DE69735382T2 (de) * | 1996-11-04 | 2006-11-30 | Qiagen Gmbh | Kationische reagenzien zür transfektion |
-
1997
- 1997-11-03 DE DE69735382T patent/DE69735382T2/de not_active Expired - Lifetime
- 1997-11-03 JP JP52084398A patent/JP4175667B2/ja not_active Expired - Lifetime
- 1997-11-03 AU AU53156/98A patent/AU738384B2/en not_active Expired
- 1997-11-03 EP EP97950064A patent/EP1003556B1/en not_active Expired - Lifetime
- 1997-11-03 CA CA002270396A patent/CA2270396C/en not_active Expired - Lifetime
- 1997-11-03 WO PCT/EP1997/006035 patent/WO1998019709A2/en not_active Ceased
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1999
- 1999-05-04 US US09/304,995 patent/US6733777B2/en not_active Expired - Lifetime
-
2003
- 2003-11-25 US US10/721,532 patent/US20040077582A1/en not_active Abandoned
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|---|---|
| US20040077582A1 (en) | 2004-04-22 |
| US20010048939A1 (en) | 2001-12-06 |
| AU738384B2 (en) | 2001-09-20 |
| AU5315698A (en) | 1998-05-29 |
| CA2270396A1 (en) | 1998-05-14 |
| EP1003556B1 (en) | 2006-03-01 |
| WO1998019709A2 (en) | 1998-05-14 |
| DE69735382D1 (de) | 2006-04-27 |
| JP4175667B2 (ja) | 2008-11-05 |
| WO1998019709A3 (en) | 1998-07-30 |
| JP2001503751A (ja) | 2001-03-21 |
| US6733777B2 (en) | 2004-05-11 |
| EP1003556A2 (en) | 2000-05-31 |
| CA2270396C (en) | 2008-03-11 |
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