DE69734063D1 - Herstellung und verwendung von normalisierten dna-bibliotheken - Google Patents

Herstellung und verwendung von normalisierten dna-bibliotheken

Info

Publication number
DE69734063D1
DE69734063D1 DE69734063T DE69734063T DE69734063D1 DE 69734063 D1 DE69734063 D1 DE 69734063D1 DE 69734063 T DE69734063 T DE 69734063T DE 69734063 T DE69734063 T DE 69734063T DE 69734063 D1 DE69734063 D1 DE 69734063D1
Authority
DE
Germany
Prior art keywords
genomic dna
normalized
environmental sample
preparation
isolated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69734063T
Other languages
English (en)
Other versions
DE69734063T2 (de
Inventor
Jay Short
Eric J Mathur
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of DE69734063D1 publication Critical patent/DE69734063D1/de
Application granted granted Critical
Publication of DE69734063T2 publication Critical patent/DE69734063T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
DE69734063T 1996-06-18 1997-06-18 Herstellung und verwendung von normalisierten dna-bibliotheken Expired - Fee Related DE69734063T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/665,565 US5763239A (en) 1996-06-18 1996-06-18 Production and use of normalized DNA libraries
US665565 1996-06-18
PCT/US1997/010748 WO1997048717A1 (en) 1996-06-18 1997-06-18 Production and use of normalized dna libraries

Publications (2)

Publication Number Publication Date
DE69734063D1 true DE69734063D1 (de) 2005-09-29
DE69734063T2 DE69734063T2 (de) 2006-06-22

Family

ID=24670627

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69734063T Expired - Fee Related DE69734063T2 (de) 1996-06-18 1997-06-18 Herstellung und verwendung von normalisierten dna-bibliotheken

Country Status (10)

Country Link
US (4) US5763239A (de)
EP (2) EP0923598B1 (de)
JP (1) JP4227196B2 (de)
AT (1) ATE302788T1 (de)
AU (1) AU718573B2 (de)
CA (1) CA2258175A1 (de)
DE (1) DE69734063T2 (de)
DK (1) DK0923598T3 (de)
ES (1) ES2248851T3 (de)
WO (1) WO1997048717A1 (de)

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WO1999045154A1 (en) 1998-03-04 1999-09-10 Diversa Corporation Production and use of normalized dna libraries

Also Published As

Publication number Publication date
EP0923598B1 (de) 2005-08-24
US6001574A (en) 1999-12-14
EP1528067A2 (de) 2005-05-04
DK0923598T3 (da) 2006-01-09
ATE302788T1 (de) 2005-09-15
WO1997048717A1 (en) 1997-12-24
AU3406697A (en) 1998-01-07
JP4227196B2 (ja) 2009-02-18
JP2000513933A (ja) 2000-10-24
AU718573B2 (en) 2000-04-13
US6444426B1 (en) 2002-09-03
EP0923598A4 (de) 2000-07-19
EP1528067A3 (de) 2006-02-01
US5763239A (en) 1998-06-09
EP0923598A1 (de) 1999-06-23
US20030104440A1 (en) 2003-06-05
CA2258175A1 (en) 1997-12-24
ES2248851T3 (es) 2006-03-16
DE69734063T2 (de) 2006-06-22

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