GB2262987A - Identification of organisms - Google Patents

Identification of organisms

Info

Publication number
GB2262987A
GB2262987A GB9302666A GB9302666A GB2262987A GB 2262987 A GB2262987 A GB 2262987A GB 9302666 A GB9302666 A GB 9302666A GB 9302666 A GB9302666 A GB 9302666A GB 2262987 A GB2262987 A GB 2262987A
Authority
GB
United Kingdom
Prior art keywords
organisms
nucleic acid
probe
identification
those
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB9302666A
Other versions
GB9302666D0 (en
Inventor
Gregory Glenn Lennon
Hans Rudolph Lehrach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cancer Research Horizons Ltd
Original Assignee
Imperial Cancer Research Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB909020621A external-priority patent/GB9020621D0/en
Application filed by Imperial Cancer Research Technology Ltd filed Critical Imperial Cancer Research Technology Ltd
Priority to GB9302666A priority Critical patent/GB2262987A/en
Publication of GB9302666D0 publication Critical patent/GB9302666D0/en
Publication of GB2262987A publication Critical patent/GB2262987A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Nucleic acid (usually PCR-amplified) from organisms is screened with a library of short probes to determine whether each probe hybridises to the nucleic acid and, if so, how many times. A "hybridisation signature" is thereby created with a score (0, 1, 2 etc) for each probe. The method can be used to provide an initial screening of bacteria gathered from the wild to weed out those of an undesired type and those studied previously, and also for typing organisms of medical or industrial interest.
GB9302666A 1990-09-21 1993-02-09 Identification of organisms Withdrawn GB2262987A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9302666A GB2262987A (en) 1990-09-21 1993-02-09 Identification of organisms

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB909020621A GB9020621D0 (en) 1990-09-21 1990-09-21 Identification of organisms
PCT/GB1991/001618 WO1992005280A1 (en) 1990-09-21 1991-09-20 Identification of organisms
GB9302666A GB2262987A (en) 1990-09-21 1993-02-09 Identification of organisms

Publications (2)

Publication Number Publication Date
GB9302666D0 GB9302666D0 (en) 1993-05-19
GB2262987A true GB2262987A (en) 1993-07-07

Family

ID=26297685

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9302666A Withdrawn GB2262987A (en) 1990-09-21 1993-02-09 Identification of organisms

Country Status (1)

Country Link
GB (1) GB2262987A (en)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2156074A (en) * 1984-02-17 1985-10-02 Orion Yhtymae Oy Improved nucleic acid reagents and methods for their preparation
WO1987002065A1 (en) * 1985-01-08 1987-04-09 Biotal Limited Determination of identity between two organisms
EP0237737A2 (en) * 1986-01-30 1987-09-23 Enzo Biochem, Inc. A composition specific for Neisseria gonorrhoea
WO1989005359A1 (en) * 1987-12-01 1989-06-15 Integrated Genetics, Inc. Detection of salmonella
WO1989006704A1 (en) * 1988-01-11 1989-07-27 Microprobe Corporation Oligonucleotide probes for detection of periodontal pathogens
EP0337896A1 (en) * 1988-04-15 1989-10-18 N.V. Innogenetics S.A. Hybridization probes for detecting neisseria strains
WO1989010977A1 (en) * 1988-05-03 1989-11-16 Isis Innovation Limited Analysing polynucleotide sequences
WO1989011547A1 (en) * 1988-05-20 1989-11-30 Cetus Corporation Method for hla dp typing
FR2636075A1 (en) * 1988-09-07 1990-03-09 Biotechnologie Ste Europ Process for the detection of bacteria, yeasts, parasites and other eurcaryotes especially in foodstuffs
EP0395292A2 (en) * 1989-04-20 1990-10-31 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2156074A (en) * 1984-02-17 1985-10-02 Orion Yhtymae Oy Improved nucleic acid reagents and methods for their preparation
WO1987002065A1 (en) * 1985-01-08 1987-04-09 Biotal Limited Determination of identity between two organisms
EP0237737A2 (en) * 1986-01-30 1987-09-23 Enzo Biochem, Inc. A composition specific for Neisseria gonorrhoea
WO1989005359A1 (en) * 1987-12-01 1989-06-15 Integrated Genetics, Inc. Detection of salmonella
WO1989006704A1 (en) * 1988-01-11 1989-07-27 Microprobe Corporation Oligonucleotide probes for detection of periodontal pathogens
EP0337896A1 (en) * 1988-04-15 1989-10-18 N.V. Innogenetics S.A. Hybridization probes for detecting neisseria strains
WO1989010977A1 (en) * 1988-05-03 1989-11-16 Isis Innovation Limited Analysing polynucleotide sequences
WO1989011547A1 (en) * 1988-05-20 1989-11-30 Cetus Corporation Method for hla dp typing
FR2636075A1 (en) * 1988-09-07 1990-03-09 Biotechnologie Ste Europ Process for the detection of bacteria, yeasts, parasites and other eurcaryotes especially in foodstuffs
EP0395292A2 (en) * 1989-04-20 1990-10-31 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Anal.Biochem. 1989,181,23-27 *
FEMS Microbiol.Lett. 1987,43,187-193 *
FEMS Microbiol.Lett. 1989,57,19-24 *
J.Gen.Microbiol. 1987,133,1969-1974 *

Also Published As

Publication number Publication date
GB9302666D0 (en) 1993-05-19

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)