JP2000513933A - 平均化dnaライブラリーの作製および使用 - Google Patents
平均化dnaライブラリーの作製および使用Info
- Publication number
- JP2000513933A JP2000513933A JP10503387A JP50338798A JP2000513933A JP 2000513933 A JP2000513933 A JP 2000513933A JP 10503387 A JP10503387 A JP 10503387A JP 50338798 A JP50338798 A JP 50338798A JP 2000513933 A JP2000513933 A JP 2000513933A
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- Japan
- Prior art keywords
- genomic dna
- library
- dna
- isolated
- population
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.自然環境試料から平均化ゲノムDNAライブラリーを作製する方法であって 、 (a)自然環境試料からゲノムDNA集団を単離する工程、 (b)前記のように単離されたゲノムDNA集団の複雑度を分析する工程、 (c)(i)前記のように単離されたDNA集団のコピー数を増幅する工程お よび(ii)所望の特性を有する単離されたゲノムDNAの画分を回収する工程の うち少なくとも一つの工程、ならびに (d)該ゲノムDNA集団内の種々のDNAの表現を平均化して自然環境試料 からゲノムDNAの平均化ライブラリーを作製する工程、 を含む前記方法。 2.所望の特性を有する単離されたゲノムDNAの画分を回収する工程を含む、 請求項1に記載の方法。 3.前記のように単離されたDNA集団のコピー数を増幅する工程を含む、請求 項1に記載の方法。 4.ゲノムDNAを増幅する工程が平均化工程の前に行われる、請求項1に記載 の方法。 5.ゲノムDNAを平均化する工程が増幅工程の前に行われる、請求項1に記載 の方法。 6.(i)前記のように単離されたDNA集団のコピー数を増幅する工程および (ii)所望の特性を有する単離されたゲノムDNAの画分を回収する工程の両方 を含む、請求項1に記載の方法。 7.(a)自然環境試料からゲノムDNA集団を単離する工程、 (b)前記のように単離されたゲノムDNA集団の複雑度を分析する工程、 (c)(i)前記のように単離されたDNA集団のコピー数を増幅する工程お よび(ii)所望の特性を有する単離されたゲノムDNAの画分を回収する工程の うち少なくとも一つの工程、ならびに (d)ゲノムDNA集団内の種々のDNAの表現を平均化して自然環境試料か らゲノムDNAの平均化ライブラリーを作製する工程、 を含む方法によって自然環境試料から作製された平均化ゲノムDNAライブラリ ー。 8.前記ライブラリーを作製する方法が、所望の特性を有する単離されたゲノム DNAの画分を回収する工程を含む、請求項1に記載のライブラリー。 9.前記ライブラリーを作製する方法が、前記のように単離されたDNA集団の コピー数を増幅する工程を含む、請求項1に記載のライブラリー。 10.前記ライブラリーを作製する方法において、ゲノムDNAを増幅する工程 が平均化工程の前に行われる、請求項1に記載のライブラリー。 11.前記ライブラリーを作製する方法において、ゲノムDNAを平均化する工 程が増幅工程の前に行われる、請求項1に記載のライブラリー。 12.前記ライブラリーを作製する方法が、(i)前記のように単離されたDN A集団のコピー数を増幅する工程および(ii)所望の特性を有する単離されたゲ ノムDNAの画分を回収する工程の両方を含む、請求項1に記載のライブラリー 13.自然環境試料からゲノム遺伝子クラスターの平均化ライブラリーを作製す る方法であって、 (a)自然環境試料からゲノムDNA集団を単離し、 (b)前記のように単離されたゲノムDNA集団の複雑度を分析し、 (c)(i)前記のように単離されたDNA集団のコピー数の増幅および(ii )所望の特性を有する単離されたゲノムDNAの画分の回収のうち少なくとも一 つを行い、そして (d)ゲノムDNA集団内の種々のDNAの表現を平均化して自然環境試料か らゲノムDNAの平均化ライブラリーを作製する、 ことを含む前記方法。 14.(a)自然環境試料からゲノムDNA集団を単離する工程、 (b)前記のように単離されたゲノムDNA集団の複雑度を分析する工程、 (c)(i)前記のように単離されたDNA集団のコピー数を増幅する工程お よび(ii)所望の特性を有する単離されたゲノムDNAの画分を回収する工程の うち少なくとも一つの工程、ならびに (d)ゲノムDNA集団内の種々のDNAの表現を平均化して自然環境試料か らゲノムDNAの平均化ライブラリーを作製する工程、 を含む方法によって自然環境試料から作製されたゲノム遺伝子クラスターの平均 化ライブラリー。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/665,565 US5763239A (en) | 1996-06-18 | 1996-06-18 | Production and use of normalized DNA libraries |
US08/665,565 | 1996-06-18 | ||
PCT/US1997/010748 WO1997048717A1 (en) | 1996-06-18 | 1997-06-18 | Production and use of normalized dna libraries |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2000513933A true JP2000513933A (ja) | 2000-10-24 |
JP4227196B2 JP4227196B2 (ja) | 2009-02-18 |
Family
ID=24670627
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50338798A Expired - Fee Related JP4227196B2 (ja) | 1996-06-18 | 1997-06-18 | 平均化dnaライブラリーの作製および使用 |
Country Status (10)
Country | Link |
---|---|
US (4) | US5763239A (ja) |
EP (2) | EP0923598B1 (ja) |
JP (1) | JP4227196B2 (ja) |
AT (1) | ATE302788T1 (ja) |
AU (1) | AU718573B2 (ja) |
CA (1) | CA2258175A1 (ja) |
DE (1) | DE69734063T2 (ja) |
DK (1) | DK0923598T3 (ja) |
ES (1) | ES2248851T3 (ja) |
WO (1) | WO1997048717A1 (ja) |
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EP0923598B1 (en) | 2005-08-24 |
JP4227196B2 (ja) | 2009-02-18 |
AU3406697A (en) | 1998-01-07 |
US5763239A (en) | 1998-06-09 |
DK0923598T3 (da) | 2006-01-09 |
EP1528067A2 (en) | 2005-05-04 |
US6001574A (en) | 1999-12-14 |
DE69734063T2 (de) | 2006-06-22 |
DE69734063D1 (de) | 2005-09-29 |
US6444426B1 (en) | 2002-09-03 |
CA2258175A1 (en) | 1997-12-24 |
US20030104440A1 (en) | 2003-06-05 |
EP0923598A4 (en) | 2000-07-19 |
ES2248851T3 (es) | 2006-03-16 |
ATE302788T1 (de) | 2005-09-15 |
AU718573B2 (en) | 2000-04-13 |
WO1997048717A1 (en) | 1997-12-24 |
EP0923598A1 (en) | 1999-06-23 |
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