EP1060269A4 - Production and use of normalized dna libraries - Google Patents

Production and use of normalized dna libraries

Info

Publication number
EP1060269A4
EP1060269A4 EP99909874A EP99909874A EP1060269A4 EP 1060269 A4 EP1060269 A4 EP 1060269A4 EP 99909874 A EP99909874 A EP 99909874A EP 99909874 A EP99909874 A EP 99909874A EP 1060269 A4 EP1060269 A4 EP 1060269A4
Authority
EP
European Patent Office
Prior art keywords
production
dna libraries
normalized dna
normalized
libraries
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99909874A
Other languages
German (de)
French (fr)
Other versions
EP1060269A1 (en
Inventor
Jay Short
Eric J Mathur
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/034,724 external-priority patent/US6001574A/en
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of EP1060269A1 publication Critical patent/EP1060269A1/en
Publication of EP1060269A4 publication Critical patent/EP1060269A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
EP99909874A 1998-03-04 1999-03-04 Production and use of normalized dna libraries Withdrawn EP1060269A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/034,724 US6001574A (en) 1996-06-18 1998-03-04 Production and use of normalized DNA libraries
US34724 1998-03-04
PCT/US1999/004917 WO1999045154A1 (en) 1998-03-04 1999-03-04 Production and use of normalized dna libraries

Publications (2)

Publication Number Publication Date
EP1060269A1 EP1060269A1 (en) 2000-12-20
EP1060269A4 true EP1060269A4 (en) 2004-10-27

Family

ID=21878199

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99909874A Withdrawn EP1060269A4 (en) 1998-03-04 1999-03-04 Production and use of normalized dna libraries

Country Status (6)

Country Link
EP (1) EP1060269A4 (en)
JP (1) JP2002505121A (en)
AU (1) AU744699B2 (en)
CA (1) CA2321930A1 (en)
MX (1) MXPA00008559A (en)
WO (1) WO1999045154A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US8476011B1 (en) * 1996-06-18 2013-07-02 Bp Corporation North America Inc. Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6399334B1 (en) * 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
JP2002522072A (en) 1998-08-12 2002-07-23 マキシジェン, インコーポレイテッド DNA shuffling of monooxygenase gene for production of industrial chemicals.
US6500639B2 (en) 1998-10-07 2002-12-31 Maxygen, Inc. DNA shuffling to produce nucleic acids for mycotoxin detoxification
AU1619400A (en) 1998-11-10 2000-05-29 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plantphenotypes
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7384387B1 (en) 1999-02-11 2008-06-10 Maxygen, Inc. High throughput mass spectrometry
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
FR2808276B1 (en) 2000-04-26 2004-04-02 Renaud Nalin METHOD FOR INDIRECT EXTRACTION OF DNA FROM NON-CULTIVABLE ORGANISMS AND DNA LIKELY TO BE OBTAINED BY SAID PROCESS
CA2411828A1 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
ATE501251T1 (en) 2001-01-25 2011-03-15 Evolva Ltd CELL LIBRARY
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1386966A1 (en) 2002-07-24 2004-02-04 Libragen Method for the expression of unknown environmental DNA into adapted host cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995008647A1 (en) * 1993-09-24 1995-03-30 The Trustees Of Columbia University In The City Of New York METHOD FOR CONSTRUCTION OF NORMALIZED cDNA LIBRARIES
WO1997048717A1 (en) * 1996-06-18 1997-12-24 Recombinant Biocatalysis, Inc. Production and use of normalized dna libraries

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995008647A1 (en) * 1993-09-24 1995-03-30 The Trustees Of Columbia University In The City Of New York METHOD FOR CONSTRUCTION OF NORMALIZED cDNA LIBRARIES
WO1997048717A1 (en) * 1996-06-18 1997-12-24 Recombinant Biocatalysis, Inc. Production and use of normalized dna libraries

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO9945154A1 *

Also Published As

Publication number Publication date
WO1999045154A1 (en) 1999-09-10
CA2321930A1 (en) 1999-09-10
AU744699B2 (en) 2002-02-28
MXPA00008559A (en) 2005-06-06
EP1060269A1 (en) 2000-12-20
JP2002505121A (en) 2002-02-19
AU2898199A (en) 1999-09-20

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