DE69732419T2 - Die verwendung von modularen oligonukleotiden als sonden oder primern in auf nukleinsaure basierenden tests - Google Patents
Die verwendung von modularen oligonukleotiden als sonden oder primern in auf nukleinsaure basierenden tests Download PDFInfo
- Publication number
- DE69732419T2 DE69732419T2 DE69732419T DE69732419T DE69732419T2 DE 69732419 T2 DE69732419 T2 DE 69732419T2 DE 69732419 T DE69732419 T DE 69732419T DE 69732419 T DE69732419 T DE 69732419T DE 69732419 T2 DE69732419 T2 DE 69732419T2
- Authority
- DE
- Germany
- Prior art keywords
- oligonucleotide
- capture
- modular
- module
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108091034117 Oligonucleotide Proteins 0.000 title claims abstract description 283
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract description 66
- 238000012360 testing method Methods 0.000 title description 7
- 235000015067 sauces Nutrition 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 92
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 91
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 91
- 238000000034 method Methods 0.000 claims abstract description 85
- 239000002773 nucleotide Substances 0.000 claims abstract description 67
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 66
- 238000009739 binding Methods 0.000 claims abstract description 64
- 230000027455 binding Effects 0.000 claims abstract description 63
- 238000001514 detection method Methods 0.000 claims abstract description 35
- 230000000295 complement effect Effects 0.000 claims abstract description 31
- 238000002955 isolation Methods 0.000 claims abstract description 21
- 238000009396 hybridization Methods 0.000 claims description 28
- 238000012163 sequencing technique Methods 0.000 claims description 27
- 239000007787 solid Substances 0.000 claims description 23
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 22
- 108010090804 Streptavidin Proteins 0.000 claims description 16
- 229960002685 biotin Drugs 0.000 claims description 16
- 239000011616 biotin Substances 0.000 claims description 16
- 235000020958 biotin Nutrition 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 6
- -1 HCV nucleic acid Chemical class 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 4
- 108020004414 DNA Proteins 0.000 description 99
- 239000000523 sample Substances 0.000 description 89
- 239000011324 bead Substances 0.000 description 45
- 241000711549 Hepacivirus C Species 0.000 description 41
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 38
- 238000003752 polymerase chain reaction Methods 0.000 description 25
- 102000053602 DNA Human genes 0.000 description 23
- 238000006243 chemical reaction Methods 0.000 description 20
- 238000002474 experimental method Methods 0.000 description 20
- 230000000694 effects Effects 0.000 description 17
- 230000005291 magnetic effect Effects 0.000 description 16
- 239000000047 product Substances 0.000 description 15
- 230000003321 amplification Effects 0.000 description 14
- 238000003199 nucleic acid amplification method Methods 0.000 description 14
- 208000005176 Hepatitis C Diseases 0.000 description 13
- 239000012634 fragment Substances 0.000 description 13
- 108020004682 Single-Stranded DNA Proteins 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 241000725303 Human immunodeficiency virus Species 0.000 description 10
- 108091093037 Peptide nucleic acid Proteins 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 238000003757 reverse transcription PCR Methods 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- 230000015572 biosynthetic process Effects 0.000 description 9
- 239000000872 buffer Substances 0.000 description 9
- 238000011534 incubation Methods 0.000 description 9
- 238000002347 injection Methods 0.000 description 9
- 239000007924 injection Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- 238000013459 approach Methods 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 7
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 7
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 7
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 7
- 238000000137 annealing Methods 0.000 description 7
- 238000010839 reverse transcription Methods 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 230000003612 virological effect Effects 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 238000012408 PCR amplification Methods 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 230000003100 immobilizing effect Effects 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 5
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 5
- 230000003993 interaction Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 239000007790 solid phase Substances 0.000 description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000007857 nested PCR Methods 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102100034343 Integrase Human genes 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 101000897979 Homo sapiens Putative spermatid-specific linker histone H1-like protein Proteins 0.000 description 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 102100021861 Putative spermatid-specific linker histone H1-like protein Human genes 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 239000012148 binding buffer Substances 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012869 ethanol precipitation Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 238000002205 phenol-chloroform extraction Methods 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 208000002109 Argyria Diseases 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000008857 Ferritin Human genes 0.000 description 1
- 108050000784 Ferritin Proteins 0.000 description 1
- 238000008416 Ferritin Methods 0.000 description 1
- 102100022653 Histone H1.5 Human genes 0.000 description 1
- 101000899879 Homo sapiens Histone H1.5 Proteins 0.000 description 1
- 238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- 229920001410 Microfiber Polymers 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 241000713869 Moloney murine leukemia virus Species 0.000 description 1
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 102000009661 Repressor Proteins Human genes 0.000 description 1
- 108010034634 Repressor Proteins Proteins 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000002079 cooperative effect Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 125000002485 formyl group Chemical group [H]C(*)=O 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 108060003552 hemocyanin Proteins 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229940059904 light mineral oil Drugs 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000003658 microfiber Substances 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 101150088264 pol gene Proteins 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001566 pro-viral effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
- C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Communicable Diseases (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Semiconductor Lasers (AREA)
- Seeds, Soups, And Other Foods (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9620075.3A GB9620075D0 (en) | 1996-09-26 | 1996-09-26 | Method |
| GB9620075 | 1996-09-26 | ||
| PCT/GB1997/002629 WO1998013522A1 (en) | 1996-09-26 | 1997-09-26 | The use of modular oligonucleotides as probes or primers in nucleic acid based assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69732419D1 DE69732419D1 (de) | 2005-03-10 |
| DE69732419T2 true DE69732419T2 (de) | 2006-04-06 |
Family
ID=10800518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69732419T Expired - Lifetime DE69732419T2 (de) | 1996-09-26 | 1997-09-26 | Die verwendung von modularen oligonukleotiden als sonden oder primern in auf nukleinsaure basierenden tests |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6482592B1 (enExample) |
| EP (1) | EP0928342B1 (enExample) |
| JP (1) | JP2001501470A (enExample) |
| AT (1) | ATE288499T1 (enExample) |
| AU (1) | AU743820B2 (enExample) |
| CA (1) | CA2266749C (enExample) |
| DE (1) | DE69732419T2 (enExample) |
| ES (1) | ES2234031T3 (enExample) |
| GB (1) | GB9620075D0 (enExample) |
| WO (1) | WO1998013522A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9620075D0 (en) | 1996-09-26 | 1996-11-13 | Dynal As | Method |
| US5888778A (en) * | 1997-06-16 | 1999-03-30 | Exact Laboratories, Inc. | High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers |
| GB9820185D0 (en) * | 1998-09-15 | 1998-11-11 | Dynal As | Method |
| AU773108B2 (en) * | 1998-09-15 | 2004-05-13 | Dynal Asa | Method of isolation primer extension products with modular oligonucleotides |
| US20010031467A1 (en) * | 1999-12-10 | 2001-10-18 | Johannes Dapprich | Method for selectively isolating a nucleic acid |
| DK1370653T3 (da) * | 2001-03-15 | 2008-01-21 | Univ Aix Marseille Ii | Fremstilling af kalibratorer og anvendelse heraf til kvantificering af nukleotidsekvenser af interesse |
| AU2004254367B2 (en) * | 2003-06-27 | 2008-06-19 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| US20050095627A1 (en) * | 2003-09-03 | 2005-05-05 | The Salk Institute For Biological Studies | Multiple antigen detection assays and reagents |
| EP1784508B1 (en) * | 2004-08-09 | 2012-10-03 | Generation Biotech, LLC | Method for nucleic acid isolation and amplification |
| WO2006047787A2 (en) | 2004-10-27 | 2006-05-04 | Exact Sciences Corporation | Method for monitoring disease progression or recurrence |
| US9777314B2 (en) | 2005-04-21 | 2017-10-03 | Esoterix Genetic Laboratories, Llc | Analysis of heterogeneous nucleic acid samples |
| WO2007133740A1 (en) * | 2006-05-15 | 2007-11-22 | Generation Biotech, Llc | Method for identification of novel physical linkage of genomic sequences |
| EP1903117A1 (en) * | 2006-09-22 | 2008-03-26 | Veterinärmedizinische Universität Wien | Methods for the detection of mutations by means of primers that hybridize contiguously |
| US8790916B2 (en) | 2009-05-14 | 2014-07-29 | Genestream, Inc. | Microfluidic method and system for isolating particles from biological fluid |
| US8747084B2 (en) | 2010-07-21 | 2014-06-10 | Aperia Technologies, Inc. | Peristaltic pump |
| WO2013116039A1 (en) * | 2012-01-31 | 2013-08-08 | Advanced Liquid Logic Inc | Amplification primers and probes for detection of hiv-1 |
| WO2013138510A1 (en) * | 2012-03-13 | 2013-09-19 | Patel Abhijit Ajit | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
| BR112014022974B1 (pt) | 2012-03-20 | 2021-08-17 | Aperia Technologies | Sistema de inflagem de pneu |
| US9604157B2 (en) | 2013-03-12 | 2017-03-28 | Aperia Technologies, Inc. | Pump with water management |
| US11453258B2 (en) | 2013-03-12 | 2022-09-27 | Aperia Technologies, Inc. | System for tire inflation |
| US10144254B2 (en) | 2013-03-12 | 2018-12-04 | Aperia Technologies, Inc. | Tire inflation system |
| ITUA20163413A1 (it) * | 2016-05-13 | 2017-11-13 | Fondazione St Italiano Tecnologia | Reazione di amplificazione di acidi nucleici con primer cooperativi. |
| CN109952237B (zh) | 2016-09-06 | 2022-08-26 | 阿佩利亚科技公司 | 用于轮胎充气的系统 |
| US10406869B2 (en) | 2017-11-10 | 2019-09-10 | Aperia Technologies, Inc. | Inflation system |
| US11642920B2 (en) | 2018-11-27 | 2023-05-09 | Aperia Technologies, Inc. | Hub-integrated inflation system |
| US12442364B2 (en) | 2023-12-11 | 2025-10-14 | Aperia Technologies, Inc. | Two-stage pump and method of operation |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5149625A (en) | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
| US5030557A (en) | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| US4988617A (en) * | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| DE68919706T2 (de) * | 1988-05-10 | 1995-04-13 | Du Pont | Verfahren zum schnellen nachweis von nukleinsäuren. |
| DE69022362T2 (de) * | 1989-05-24 | 1996-05-09 | Amoco Corp | Nukleinsäuresonden für die detektion von neisseria gonorrhoeae. |
| FR2663040B1 (fr) * | 1990-06-11 | 1995-09-15 | Bio Merieux | Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich. |
| JPH0484899A (ja) * | 1990-07-26 | 1992-03-18 | Tosoh Corp | 核酸測定法 |
| US5627032A (en) * | 1990-12-24 | 1997-05-06 | Ulanovsky; Levy | Composite primers for nucleic acids |
| US5428145A (en) | 1991-08-09 | 1995-06-27 | Immuno Japan, Inc. | Non-A, non-B, hepatitis virus genome, polynucleotides, polypeptides, antigen, antibody and detection systems |
| US5387510A (en) | 1991-10-02 | 1995-02-07 | Eastman Kodak Company | Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit |
| GB9207598D0 (en) | 1992-04-03 | 1992-05-20 | Dynal As | Method of sequencing double stranded dna |
| JP3408564B2 (ja) * | 1992-04-30 | 2003-05-19 | メディカル リサーチ カウンスル | 結核菌中のイソニアジド抵抗性の迅速検出 |
| US5547843A (en) * | 1992-07-17 | 1996-08-20 | Associated Universities, Inc. | Method for promoting specific alignment of short oligonucleotides on nucleic acids |
| JP3454847B2 (ja) * | 1992-08-26 | 2003-10-06 | 株式会社東芝 | ハイブリダイゼーション法 |
| CA2119701A1 (en) * | 1993-04-16 | 1994-10-17 | Colleen M. Nycz | Nucleic acid assay procedure |
| JP3746083B2 (ja) * | 1993-05-28 | 2006-02-15 | 日本製粉株式会社 | 隣接する２種の核酸プローブを用いたｒｎａの検出方法 |
| CA2170264A1 (en) | 1993-09-10 | 1995-03-16 | Michael W. Konrad | Optical detection of position of oligonucleotides on large dna molecules |
| ES2176342T5 (es) * | 1994-10-21 | 2010-02-09 | Innogenetics N.V. | Secuencias del genotipo 7 del virus de la hepatitis c y su utilizacion como agentes profilacticos, terapeuticos y de diagnosticos. |
| US5731153A (en) * | 1996-08-26 | 1998-03-24 | The Regents Of The University Of California | Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions |
| US5908745A (en) * | 1996-01-16 | 1999-06-01 | University Of Chicago | Use of continuous/contiguous stacking hybridization as a diagnostic tool |
| GB9620075D0 (en) | 1996-09-26 | 1996-11-13 | Dynal As | Method |
| US6124092A (en) | 1996-10-04 | 2000-09-26 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
| GB9820185D0 (en) * | 1998-09-15 | 1998-11-11 | Dynal As | Method |
-
1996
- 1996-09-26 GB GBGB9620075.3A patent/GB9620075D0/en active Pending
-
1997
- 1997-09-26 AT AT97942121T patent/ATE288499T1/de not_active IP Right Cessation
- 1997-09-26 ES ES97942121T patent/ES2234031T3/es not_active Expired - Lifetime
- 1997-09-26 EP EP97942121A patent/EP0928342B1/en not_active Expired - Lifetime
- 1997-09-26 DE DE69732419T patent/DE69732419T2/de not_active Expired - Lifetime
- 1997-09-26 CA CA002266749A patent/CA2266749C/en not_active Expired - Fee Related
- 1997-09-26 AU AU43917/97A patent/AU743820B2/en not_active Ceased
- 1997-09-26 WO PCT/GB1997/002629 patent/WO1998013522A1/en not_active Ceased
- 1997-09-26 JP JP10515411A patent/JP2001501470A/ja not_active Ceased
-
1998
- 1998-09-15 US US09/153,242 patent/US6482592B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU743820B2 (en) | 2002-02-07 |
| EP0928342A1 (en) | 1999-07-14 |
| DE69732419D1 (de) | 2005-03-10 |
| CA2266749C (en) | 2009-11-10 |
| AU4391797A (en) | 1998-04-17 |
| WO1998013522A1 (en) | 1998-04-02 |
| ES2234031T3 (es) | 2005-06-16 |
| CA2266749A1 (en) | 1998-04-02 |
| EP0928342B1 (en) | 2005-02-02 |
| US6482592B1 (en) | 2002-11-19 |
| JP2001501470A (ja) | 2001-02-06 |
| ATE288499T1 (de) | 2005-02-15 |
| GB9620075D0 (en) | 1996-11-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69732419T2 (de) | Die verwendung von modularen oligonukleotiden als sonden oder primern in auf nukleinsaure basierenden tests | |
| DE69122457T2 (de) | Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation | |
| DE69432419T2 (de) | Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation | |
| DE69128077T2 (de) | Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen | |
| DE3852177T2 (de) | Transkriptionsbasierte nukleinsäure-verstärkungs/nachweissysteme. | |
| DE69625461T2 (de) | Fortführende amplifizierungsreaktion | |
| DE69523360T2 (de) | Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung | |
| DE68911648T2 (de) | Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird. | |
| DE69432709T2 (de) | Nukleinsäure - Sandwichassay in Lösungsphase mit reduzierten Hintergrundsignalen | |
| DE69327326T2 (de) | Vervielfältigungs- und detektionsprozess | |
| DE69836012T2 (de) | Zwei-schritt hybridisierung und einfang von einem polynukleotid | |
| DE68926484T2 (de) | RNS-Template mit endverbundener Sonde und Verfahren zur Verwendung | |
| DE69028325T2 (de) | Nukleinsäure-Amplifikation unter Verwendung eines Einzelprimers | |
| DE69516116T2 (de) | Sequenzierung von nukleinsäuren | |
| EP0718408A2 (de) | Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren | |
| DE69029740T2 (de) | Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe | |
| DE69902086T2 (de) | Verfahren zum nachweis von nukleinsäure-zielsequenzen mittels in vitro transkription von einem rna-promoter | |
| EP1029084A2 (de) | Spezifisches und sensitives nukleinsäurenachweisverfahren | |
| DE60308017T2 (de) | Amplifizierungs-hybridisierungsverfahren zum nachweis und zur typisierung des mes menschlichen papillomavirus | |
| DE202017007129U1 (de) | Lysereagenz für Blutzellen | |
| EP0437774B1 (de) | Verfahren zur Herstellung von modifizierten Nukleinsäuren | |
| DE112006002652T5 (de) | Verfahren zur isothermen Amplifikation von Nukleinsäuren und Verfahren zum Nachweisen von Nukleinsäuren durch gleichzeitige isotherme Amplifikation von Nukleinsäuren und Signalsonde | |
| KR102679112B1 (ko) | 분할된 t7 프로모터를 이용한 등온 단일 반응용 프로브 세트 및 이의 용도 | |
| EP0552203B1 (de) | Verfahren zum empfindlichen nachweis von nukleinsäuren | |
| DE10051564A1 (de) | Neue Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |