DE69722176T2 - Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen - Google Patents

Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen Download PDF

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Publication number
DE69722176T2
DE69722176T2 DE69722176T DE69722176T DE69722176T2 DE 69722176 T2 DE69722176 T2 DE 69722176T2 DE 69722176 T DE69722176 T DE 69722176T DE 69722176 T DE69722176 T DE 69722176T DE 69722176 T2 DE69722176 T2 DE 69722176T2
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Prior art keywords
cells
cell
peptide
randomized
sequence
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Expired - Lifetime
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DE69722176T
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English (en)
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DE69722176D1 (de
Inventor
Garry P. Noaln
S. Michael Rothenberg
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Rigel Pharmaceuticals Inc
Leland Stanford Junior University
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Rigel Pharmaceuticals Inc
Leland Stanford Junior University
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Priority claimed from US08/589,109 external-priority patent/US6365344B1/en
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Publication of DE69722176T2 publication Critical patent/DE69722176T2/de
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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DE69722176T 1996-01-23 1997-01-23 Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen Expired - Lifetime DE69722176T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US58991196A 1996-01-23 1996-01-23
US08/589,109 US6365344B1 (en) 1996-01-23 1996-01-23 Methods for screening for transdominant effector peptides and RNA molecules
US589109 1996-01-23
US589911 1996-01-23
PCT/US1997/001048 WO1997027213A1 (en) 1996-01-23 1997-01-23 Methods for screening for transdominant effector peptides and rna molecules

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DE69722176D1 DE69722176D1 (de) 2003-06-26
DE69722176T2 true DE69722176T2 (de) 2004-03-18

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DE69722176T Expired - Lifetime DE69722176T2 (de) 1996-01-23 1997-01-23 Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen

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US (5) US6455247B1 (enExample)
EP (2) EP0877752B1 (enExample)
JP (2) JP2000504220A (enExample)
AU (2) AU725716C (enExample)
CA (1) CA2244222A1 (enExample)
DE (1) DE69722176T2 (enExample)
DK (1) DK0877752T3 (enExample)
ES (1) ES2200150T3 (enExample)
WO (2) WO1997027212A1 (enExample)

Families Citing this family (139)

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Publication number Priority date Publication date Assignee Title
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
EP1405911A1 (en) * 1994-07-20 2004-04-07 The General Hospital Corporation Interaction trap systems for detecting protein interactions
USRE39229E1 (en) 1994-08-20 2006-08-08 Gendaq Limited Binding proteins for recognition of DNA
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
US20050002902A1 (en) * 1995-12-28 2005-01-06 Liming Yu Hybrid with interferon-alpha and an immunoglobulin Fc for treatment of tumors and viral infections
US6365344B1 (en) * 1996-01-23 2002-04-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and RNA molecules
DE69722176T2 (de) 1996-01-23 2004-03-18 The Board Of Trustees Of The Leland Stanford Junior University, Palo Alto Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen
US5955275A (en) 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US6623922B1 (en) 1997-02-14 2003-09-23 Deltagen Proteomics Methods for identifying, characterizing, and evolving cell-type specific CIS regulatory elements
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