DE69635109D1 - Verstärkung der alkalischen phosphatase durch sds als chemilumineszsubstrat und verfahren zur enzyminhibition - Google Patents
Verstärkung der alkalischen phosphatase durch sds als chemilumineszsubstrat und verfahren zur enzyminhibitionInfo
- Publication number
- DE69635109D1 DE69635109D1 DE69635109T DE69635109T DE69635109D1 DE 69635109 D1 DE69635109 D1 DE 69635109D1 DE 69635109 T DE69635109 T DE 69635109T DE 69635109 T DE69635109 T DE 69635109T DE 69635109 D1 DE69635109 D1 DE 69635109D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- acid sequence
- bound
- target
- label
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/472,756 US5780227A (en) | 1995-06-07 | 1995-06-07 | Oligonucleotide probe conjugated to a purified hydrophilic alkaline phosphatase and uses thereof |
US08/610,955 US5853974A (en) | 1995-06-07 | 1996-03-05 | Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates |
PCT/US1996/009767 WO1996041015A2 (en) | 1995-06-07 | 1996-06-07 | Enhancement of alkaline phosphatase with sds in chemiluminescent substrates and enzyme inhibition assays |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69635109D1 true DE69635109D1 (de) | 2005-09-29 |
DE69635109T2 DE69635109T2 (de) | 2006-06-14 |
Family
ID=27043908
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69637766T Expired - Lifetime DE69637766D1 (de) | 1995-06-07 | 1996-06-07 | Erstärkung der alkalischen Phosphatase durch SDSds als Chemilumineszsubstrat und Verfahren zur Enzyminhibition |
DE69635109T Expired - Lifetime DE69635109T2 (de) | 1995-06-07 | 1996-06-07 | Verstärkung der alkalischen phosphatase durch sds als chemilumineszsubstrat und verfahren zur enzyminhibition |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69637766T Expired - Lifetime DE69637766D1 (de) | 1995-06-07 | 1996-06-07 | Erstärkung der alkalischen Phosphatase durch SDSds als Chemilumineszsubstrat und Verfahren zur Enzyminhibition |
Country Status (8)
Country | Link |
---|---|
US (1) | US5853974A (de) |
EP (2) | EP1616966B1 (de) |
JP (1) | JPH11507531A (de) |
AT (2) | ATE302856T1 (de) |
AU (1) | AU716770B2 (de) |
DE (2) | DE69637766D1 (de) |
ES (2) | ES2315769T3 (de) |
WO (1) | WO1996041015A2 (de) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9622524D0 (en) | 1996-10-29 | 1997-01-08 | London Biotechnology Ltd | Enzyme labels for assays |
US6783965B1 (en) | 2000-02-10 | 2004-08-31 | Mountain View Pharmaceuticals, Inc. | Aggregate-free urate oxidase for preparation of non-immunogenic polymer conjugates |
CA2374393A1 (en) * | 2000-03-23 | 2001-09-27 | Lumigen, Inc. | Methods of detecting polynucleotide kinase and its use as a label |
JP4576752B2 (ja) * | 2001-05-29 | 2010-11-10 | 富士レビオ株式会社 | 化学発光増強剤 |
US20040018495A1 (en) * | 2002-07-24 | 2004-01-29 | Xing-Xiang Li | Microparticle based signal amplification for the detection of analytes |
JP5414424B2 (ja) * | 2009-08-31 | 2014-02-12 | シスメックス株式会社 | 目的物質の検出方法、及び目的物質を検出するためのクロマトグラフィー用試験キット |
CN113008879B (zh) * | 2021-02-25 | 2023-05-09 | 陕西科技大学 | 一种利用反蛋白石水凝胶薄膜检测碱性磷酸酶的方法 |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1260372A (en) * | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Hybridization method for the detection of genetic materials |
US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US5707559A (en) * | 1986-07-17 | 1998-01-13 | Tropix, Inc. | Chemiluminescent 1,2-dioxetane compounds |
US5112960A (en) * | 1989-07-17 | 1992-05-12 | Bronstein Irena Y | Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes |
CA1340590C (en) * | 1986-07-24 | 1999-06-08 | John C. Voyta | Chemiluminescence enhancement |
US4978614A (en) * | 1988-10-26 | 1990-12-18 | Tropix, Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
ZA877772B (en) | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
CA1339351C (en) * | 1987-10-15 | 1997-08-26 | Michael S. Urdea | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
US5094939A (en) | 1988-07-19 | 1992-03-10 | Fujirebio, Inc. | Chemiluminescence assays using stabilized dioxetane derivatives |
US5082780A (en) * | 1989-09-12 | 1992-01-21 | Eastman Kodak Company | Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures |
US5254469A (en) * | 1989-09-12 | 1993-10-19 | Eastman Kodak Company | Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures |
CA2025178A1 (en) | 1989-10-02 | 1991-04-03 | William G. Weisburg | Nucleic acid probes for the detection of lyme disease spirochetes |
WO1992000226A1 (de) | 1990-06-27 | 1992-01-09 | Wilhelm Ernst | Eimer mit mitteln zur erhöhung seiner standfestigkeit |
JPH06502766A (ja) | 1990-11-14 | 1994-03-31 | アクゾ・ノベル・ナムローゼ・フェンノートシャップ | ポリスチレン支持体ベース−サンドイッチハイブリダイゼーションアッセイ法を用いる核酸の非同位体検出およびそれに用いる組成物 |
JPH0579985A (ja) | 1991-09-24 | 1993-03-30 | Kyoto Daiichi Kagaku:Kk | 化学発光増強剤及び増強方法 |
JP3907201B2 (ja) * | 1991-12-23 | 2007-04-18 | カイロン コーポレイション | 溶液相サンドイッチハイブリダイゼーションアッセイに用いるためのhivプローブ |
US5747244A (en) | 1991-12-23 | 1998-05-05 | Chiron Corporation | Nucleic acid probes immobilized on polystyrene surfaces |
AU3467293A (en) * | 1991-12-23 | 1993-07-28 | Chiron Corporation | (Chlamydiae) probes for use in solution phase sandwich hybridization assays |
WO1993013227A1 (en) * | 1991-12-23 | 1993-07-08 | Chiron Corporation | Cmv probes for use in solution phase sandwich hybridization assays |
CA2119701A1 (en) | 1993-04-16 | 1994-10-17 | Colleen M. Nycz | Nucleic acid assay procedure |
US5451347A (en) * | 1993-06-24 | 1995-09-19 | Lumigen, Inc. | Methods and compositions providing enhanced chemiluminescence from chemiluminescent compounds using dicationic surfactants |
US5578253A (en) * | 1994-11-23 | 1996-11-26 | Lumigen, Inc. | Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use |
US5747248A (en) | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
-
1996
- 1996-03-05 US US08/610,955 patent/US5853974A/en not_active Expired - Lifetime
- 1996-06-07 DE DE69637766T patent/DE69637766D1/de not_active Expired - Lifetime
- 1996-06-07 AU AU62663/96A patent/AU716770B2/en not_active Expired
- 1996-06-07 ES ES05018281T patent/ES2315769T3/es not_active Expired - Lifetime
- 1996-06-07 AT AT96921438T patent/ATE302856T1/de not_active IP Right Cessation
- 1996-06-07 ES ES96921438T patent/ES2247605T3/es not_active Expired - Lifetime
- 1996-06-07 JP JP9501991A patent/JPH11507531A/ja active Pending
- 1996-06-07 EP EP05018281A patent/EP1616966B1/de not_active Expired - Lifetime
- 1996-06-07 AT AT05018281T patent/ATE415494T1/de not_active IP Right Cessation
- 1996-06-07 EP EP96921438A patent/EP0832296B1/de not_active Expired - Lifetime
- 1996-06-07 WO PCT/US1996/009767 patent/WO1996041015A2/en active IP Right Grant
- 1996-06-07 DE DE69635109T patent/DE69635109T2/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
ES2315769T3 (es) | 2009-04-01 |
JPH11507531A (ja) | 1999-07-06 |
DE69635109T2 (de) | 2006-06-14 |
EP1616966A2 (de) | 2006-01-18 |
WO1996041015A2 (en) | 1996-12-19 |
EP0832296B1 (de) | 2005-08-24 |
ATE415494T1 (de) | 2008-12-15 |
ES2247605T3 (es) | 2006-03-01 |
AU6266396A (en) | 1996-12-30 |
ATE302856T1 (de) | 2005-09-15 |
DE69637766D1 (de) | 2009-01-08 |
EP1616966A3 (de) | 2007-10-03 |
US5853974A (en) | 1998-12-29 |
WO1996041015A3 (en) | 1997-05-01 |
AU716770B2 (en) | 2000-03-09 |
EP1616966B1 (de) | 2008-11-26 |
EP0832296A2 (de) | 1998-04-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC. (N.D.GES.D, US |
|
8328 | Change in the person/name/address of the agent |
Representative=s name: MAIER, D., DIPL.-ING. UNIV., PAT.-ASS., 81739 MUEN |