DE69328487T2 - Alkalische protease und verfahren zu ihrer herstellung - Google Patents

Alkalische protease und verfahren zu ihrer herstellung

Info

Publication number
DE69328487T2
DE69328487T2 DE69328487T DE69328487T DE69328487T2 DE 69328487 T2 DE69328487 T2 DE 69328487T2 DE 69328487 T DE69328487 T DE 69328487T DE 69328487 T DE69328487 T DE 69328487T DE 69328487 T2 DE69328487 T2 DE 69328487T2
Authority
DE
Germany
Prior art keywords
pct
alkaline protease
production
sec
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69328487T
Other languages
English (en)
Other versions
DE69328487D1 (de
Inventor
Helle Outtrup
Claus Dambmann
Dorrit Anita Aaslyng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of DE69328487D1 publication Critical patent/DE69328487D1/de
Publication of DE69328487T2 publication Critical patent/DE69328487T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
DE69328487T 1992-05-27 1993-05-26 Alkalische protease und verfahren zu ihrer herstellung Expired - Lifetime DE69328487T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK92702A DK70292D0 (da) 1992-05-27 1992-05-27 Nyt enzym
PCT/DK1993/000183 WO1993024623A1 (en) 1992-05-27 1993-05-26 Alkaline protease and process for its production

Publications (2)

Publication Number Publication Date
DE69328487D1 DE69328487D1 (de) 2000-05-31
DE69328487T2 true DE69328487T2 (de) 2001-01-11

Family

ID=8096553

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69328487T Expired - Lifetime DE69328487T2 (de) 1992-05-27 1993-05-26 Alkalische protease und verfahren zu ihrer herstellung

Country Status (10)

Country Link
US (1) US5597720A (de)
EP (1) EP0652947B1 (de)
JP (1) JP3309985B2 (de)
KR (1) KR100284246B1 (de)
AT (1) ATE192190T1 (de)
DE (1) DE69328487T2 (de)
DK (2) DK70292D0 (de)
ES (1) ES2148230T3 (de)
FI (1) FI117759B (de)
WO (1) WO1993024623A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5621089A (en) * 1992-05-27 1997-04-15 Novo Nordisk Biotech, Inc. Nucleic acid constructs for the production of a Bacillus alkaline protease
CN1093174C (zh) * 1995-02-10 2002-10-23 诺沃奇梅兹有限公司 芽孢杆菌属蛋白酶
WO1996024666A1 (en) * 1995-02-10 1996-08-15 Novo Nordisk A/S Bacillus proteases
DE19535082A1 (de) 1995-09-21 1997-03-27 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
DE19636035A1 (de) 1996-09-05 1998-03-12 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
US6416756B1 (en) * 1997-01-10 2002-07-09 Novozymes A/S Modified protease having 5 to 13 covalently coupled polymeric molecules for skin care
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
DE19857687A1 (de) 1998-12-15 2000-06-21 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Waschmittel
US6855548B2 (en) * 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US6960462B2 (en) * 2000-02-08 2005-11-01 Dsm Ip Assets B.V Use of acid-stable subtilisin proteases in animal feed
KR100465852B1 (ko) * 2002-06-29 2005-01-13 인하대학교 산학협력단 고활성의 알카리성 단백질 분해효소를 대량 생산하는 바실러스 속 ｉ-５２
WO2023110639A1 (en) 2021-12-16 2023-06-22 Novozymes A/S Protease animal feed formulation

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2026092C3 (de) * 1969-05-31 1979-04-12 Rikagaku Kenkyusho, Saitama (Japan) Verfahren zur Herstellung einer alkalischen Protease
JPS6055118B2 (ja) * 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
BE897479A (fr) * 1983-08-08 1983-12-01 Showa Denko Kk Protease alcaline sa preparation et son utilisation
US4771003A (en) * 1985-10-22 1988-09-13 Genex Corporation Heat stable alkaline proteases produced by a bacillus
US4764470A (en) * 1986-02-05 1988-08-16 Genex Corporation Alkaline protease produced by a bacillus
JPH01101884A (ja) * 1987-10-14 1989-04-19 Lion Corp 新規アルカリプロテアーゼｌおよびその製造法
JPH02195878A (ja) * 1989-01-24 1990-08-02 Toagosei Chem Ind Co Ltd プロテアーゼ
DK58591D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DK58491D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
JPH06261751A (ja) * 1992-03-12 1994-09-20 Japan Tobacco Inc 新規プロテアーゼ及びそれを生産するバチルス バディウス ｊｔ０１２７菌株

Also Published As

Publication number Publication date
FI945543A0 (fi) 1994-11-25
KR950701682A (ko) 1995-04-28
FI945543A (fi) 1994-11-25
ES2148230T3 (es) 2000-10-16
DK70292D0 (da) 1992-05-27
KR100284246B1 (ko) 2001-03-02
US5597720A (en) 1997-01-28
ATE192190T1 (de) 2000-05-15
EP0652947A1 (de) 1995-05-17
DE69328487D1 (de) 2000-05-31
DK0652947T3 (da) 2000-09-18
EP0652947B1 (de) 2000-04-26
FI117759B (fi) 2007-02-15
JP3309985B2 (ja) 2002-07-29
WO1993024623A1 (en) 1993-12-09
JPH07506972A (ja) 1995-08-03

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: NOVOZYMES A/S, BAGSVAERD, DK