DE69231939D1 - Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren - Google Patents

Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren

Info

Publication number
DE69231939D1
DE69231939D1 DE69231939T DE69231939T DE69231939D1 DE 69231939 D1 DE69231939 D1 DE 69231939D1 DE 69231939 T DE69231939 T DE 69231939T DE 69231939 T DE69231939 T DE 69231939T DE 69231939 D1 DE69231939 D1 DE 69231939D1
Authority
DE
Germany
Prior art keywords
nitrile
acids
methods
vectors containing
plasmid vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69231939T
Other languages
English (en)
Other versions
DE69231939T2 (de
Inventor
Teruhiko Beppu
Sueharu Horinouchi
Makoto Nishiyama
Fujio Yu
Yoshihiro Hashimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Rayon Co Ltd
Original Assignee
Mitsubishi Rayon Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Rayon Co Ltd filed Critical Mitsubishi Rayon Co Ltd
Publication of DE69231939D1 publication Critical patent/DE69231939D1/de
Application granted granted Critical
Publication of DE69231939T2 publication Critical patent/DE69231939T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69231939T 1991-03-04 1992-03-03 Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren Expired - Lifetime DE69231939T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP3754591 1991-03-04
JP3754491 1991-03-04

Publications (2)

Publication Number Publication Date
DE69231939D1 true DE69231939D1 (de) 2001-08-23
DE69231939T2 DE69231939T2 (de) 2002-04-04

Family

ID=26376666

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69231939T Expired - Lifetime DE69231939T2 (de) 1991-03-04 1992-03-03 Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren

Country Status (3)

Country Link
US (1) US5654180A (de)
EP (1) EP0502476B1 (de)
DE (1) DE69231939T2 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3692593A (en) * 1992-04-28 1993-11-04 Mitsubishi Kasei Corporation Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
FR2708936B1 (fr) * 1993-08-10 1995-11-10 Rhone Poulenc Chimie Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
JP3235934B2 (ja) * 1994-08-04 2001-12-04 三菱レイヨン株式会社 ロドコッカス属細菌由来カナマイシン耐性遺伝子
JP3828590B2 (ja) * 1994-10-03 2006-10-04 昌 清水 ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
JP3154633B2 (ja) * 1994-12-28 2001-04-09 三菱レイヨン株式会社 ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
JP3354757B2 (ja) * 1995-07-20 2002-12-09 三菱レイヨン株式会社 環状プラスミド
JP3709422B2 (ja) * 1995-07-21 2005-10-26 昌 清水 ニトリラーゼ遺伝子発現に関わる調節因子およびその遺伝子
EP0839190A1 (de) * 1995-08-09 1998-05-06 Ciba Specialty Chemicals Water Treatments Limited Verfahren zur herstellung von amidasen
AU7514896A (en) * 1995-10-06 1997-04-28 E.I. Du Pont De Nemours And Company Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant microorganisms expressing those enzymes useful for the production of chiral amides and acides
US5728556A (en) * 1996-03-14 1998-03-17 E. I. Du Pont De Nemours And Company Production of ω-cyanocarboxamides from aliphatic α,ω-dinitriles using pseudomonas putida-derived biocatalysts
US6060265A (en) * 1996-12-18 2000-05-09 Cytec Technology Corporation Methods for the detoxification of nitrile and/or amide compounds
US5863750A (en) * 1996-12-18 1999-01-26 Cytec Tech Corp Methods for the detoxification of nitrile and/or amide compounds
EA200100718A1 (ru) 1999-10-26 2001-12-24 Сова Денко К.К. Новая бактерия rhodococcus, ген нитрилазы, ген нитрилгидратазы и ген амидазы бактерии rhodococcus и способ получения карбоновых кислот с их использованием
US7521216B2 (en) 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
US7300775B2 (en) 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US6562603B2 (en) 2000-08-04 2003-05-13 E. I. Du Pont De Nemours And Company 3-hydroxycarboxylic acid production and use in branched polymers
DE60133130T2 (de) * 2000-12-20 2009-02-26 Dia-Nitrix Co.Ltd. Verfahren zur herstellung einer amidverbindung unter verwendung eines mikrobiellen katalysators
JP4733298B2 (ja) 2001-07-05 2011-07-27 三菱レイヨン株式会社 プラスミドの自律増殖に関する機能を有する遺伝子を含むｄｎａ断片
ITMI20011826A1 (it) * 2001-08-30 2003-03-02 Istituto Biochimico Italiano Microorganismo dotato di attivita' nitrile-idratasica/amidasica enantioselettiva e regioselettiva
WO2003106415A2 (en) 2002-06-13 2003-12-24 Diversa Corporation Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid
SG128684A1 (en) 2003-10-31 2007-01-30 Daiichi Fine Chem Co Ltd Novel sequence and utilization thereof
KR100738002B1 (ko) 2005-02-16 2007-07-13 주식회사 엘지화학 로도코커스―대장균 셔틀벡터
US9217164B2 (en) 2007-03-01 2015-12-22 Basf Enzymes Llc Nitrilases, nucleic acids encoding them and methods for making and using them
EP2147105B1 (de) 2007-05-02 2013-04-24 Merial Limited Dna-plasmide mit verbesserter expression und stabilität
EP2894224B1 (de) 2012-09-10 2021-09-01 Mitsubishi Chemical Corporation Verfahren zur herstellung von methacrylsäure und/oder einem ester davon
CN111378640A (zh) * 2020-03-12 2020-07-07 东莞市东阳光生物合成药有限公司 制备右旋酰胺酮洛芬的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4920054A (en) * 1986-07-25 1990-04-24 Allelix, Inc. Shuttle vectors from rhodococcus equi
US4952500A (en) * 1988-02-01 1990-08-28 University Of Georgia Research Foundation, Inc. Cloning systems for Rhodococcus and related bacteria
JP2840253B2 (ja) * 1988-07-06 1998-12-24 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法
AU627648B2 (en) * 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
US5246857A (en) * 1990-10-11 1993-09-21 Nitto Chemical Industry Co., Ltd. Circular plasmids derived from the genus rhodococcus

Also Published As

Publication number Publication date
US5654180A (en) 1997-08-05
EP0502476A2 (de) 1992-09-09
DE69231939T2 (de) 2002-04-04
EP0502476A3 (en) 1993-04-21
EP0502476B1 (de) 2001-07-18

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