DE69129773T2 - Gereinigte thermostabile DNS-Polymerase zu erhalten aus Thermococcus litoralis - Google Patents

Gereinigte thermostabile DNS-Polymerase zu erhalten aus Thermococcus litoralis

Info

Publication number
DE69129773T2
DE69129773T2 DE69129773T DE69129773T DE69129773T2 DE 69129773 T2 DE69129773 T2 DE 69129773T2 DE 69129773 T DE69129773 T DE 69129773T DE 69129773 T DE69129773 T DE 69129773T DE 69129773 T2 DE69129773 T2 DE 69129773T2
Authority
DE
Germany
Prior art keywords
dna polymerase
thermostable dna
thermococcus litoralis
polymerase obtained
purified thermostable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69129773T
Other languages
English (en)
Other versions
DE69129773D1 (de
Inventor
Donald G Comb
Francine Perler
Rebecca Kucera
William E Jack
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/513,994 external-priority patent/US5210036A/en
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of DE69129773D1 publication Critical patent/DE69129773D1/de
Publication of DE69129773T2 publication Critical patent/DE69129773T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69129773T 1990-04-26 1991-04-26 Gereinigte thermostabile DNS-Polymerase zu erhalten aus Thermococcus litoralis Expired - Lifetime DE69129773T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/513,994 US5210036A (en) 1990-04-26 1990-04-26 Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US62605790A 1990-12-11 1990-12-11
US07/686,340 US5322785A (en) 1990-04-26 1991-04-17 Purified thermostable DNA polymerase obtainable from thermococcus litoralis

Publications (2)

Publication Number Publication Date
DE69129773D1 DE69129773D1 (de) 1998-08-20
DE69129773T2 true DE69129773T2 (de) 1999-03-04

Family

ID=27414563

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69129773T Expired - Lifetime DE69129773T2 (de) 1990-04-26 1991-04-26 Gereinigte thermostabile DNS-Polymerase zu erhalten aus Thermococcus litoralis
DE91303787T Pending DE455430T1 (de) 1990-04-26 1991-04-26 Gereinigte thermostabile DNS-Polymerase zu erhalten aus Thermococcus litoralis.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE91303787T Pending DE455430T1 (de) 1990-04-26 1991-04-26 Gereinigte thermostabile DNS-Polymerase zu erhalten aus Thermococcus litoralis.

Country Status (4)

Country Link
US (1) US5322785A (de)
EP (1) EP0455430B1 (de)
JP (1) JP3229886B2 (de)
DE (2) DE69129773T2 (de)

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Also Published As

Publication number Publication date
DE69129773D1 (de) 1998-08-20
EP0455430A3 (en) 1992-02-26
EP0455430A2 (de) 1991-11-06
US5322785A (en) 1994-06-21
EP0455430B1 (de) 1998-07-15
DE455430T1 (de) 1994-02-03
JP3229886B2 (ja) 2001-11-19
JPH0568547A (ja) 1993-03-23

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