DE69033531T2 - Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen - Google Patents

Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen

Info

Publication number
DE69033531T2
DE69033531T2 DE69033531T DE69033531T DE69033531T2 DE 69033531 T2 DE69033531 T2 DE 69033531T2 DE 69033531 T DE69033531 T DE 69033531T DE 69033531 T DE69033531 T DE 69033531T DE 69033531 T2 DE69033531 T2 DE 69033531T2
Authority
DE
Germany
Prior art keywords
gene
site
lambda
downstream
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69033531T
Other languages
English (en)
Other versions
DE69033531D1 (de
Inventor
Jane Eccles
Gerardus Grosveld
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council filed Critical Medical Research Council
Application granted granted Critical
Publication of DE69033531D1 publication Critical patent/DE69033531D1/de
Publication of DE69033531T2 publication Critical patent/DE69033531T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Image Analysis (AREA)
  • Devices For Executing Special Programs (AREA)
DE69033531T 1989-02-22 1990-02-22 Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen Expired - Fee Related DE69033531T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898904009A GB8904009D0 (en) 1989-02-22 1989-02-22 Vector
PCT/GB1990/000286 WO1990010077A1 (en) 1989-02-22 1990-02-22 Vector for integration site independent gene expression in mammalian host cells

Publications (2)

Publication Number Publication Date
DE69033531D1 DE69033531D1 (de) 2000-06-08
DE69033531T2 true DE69033531T2 (de) 2001-01-11

Family

ID=10652102

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69033531T Expired - Fee Related DE69033531T2 (de) 1989-02-22 1990-02-22 Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen

Country Status (10)

Country Link
EP (2) EP0962534A3 (de)
JP (1) JPH04505552A (de)
AT (1) ATE192498T1 (de)
AU (2) AU5161490A (de)
CA (2) CA2050920A1 (de)
DE (1) DE69033531T2 (de)
DK (1) DK0460042T3 (de)
ES (1) ES2150411T3 (de)
GB (1) GB8904009D0 (de)
WO (1) WO1990010077A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9027917D0 (en) * 1990-12-21 1991-02-13 Ici Plc Expression systems
DE4228162C1 (de) 1992-08-25 1994-01-13 Rajewsky Klaus Dr Verfahren zum Ersetzen homologer Genabschnitte aus Säugern in der Keimbahn von nicht-menschlichen Säugern
DE19541450C2 (de) * 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Genkonstrukt und dessen Verwendung
CZ2001584A3 (cs) 1998-08-18 2002-06-12 The Regents Of The University Of California Prevence tvorby hlenu v dýchacích cestách podáváním antagonistů EGF-R
WO2001027150A2 (en) 1999-10-14 2001-04-19 Clontech Laboratories Inc. Anthozoa derived chromo/fluoroproteins and methods for using the same
JP2003000243A (ja) 2001-06-25 2003-01-07 Takachika Azuma 変異体の製造法
ES2329666T3 (es) 2001-12-19 2009-11-30 The University Of Chicago Proteinas fluorescentes de maduracion rapida y procedimientos de uso de las mismas.
US7585501B2 (en) 2002-06-14 2009-09-08 Stowers Institute For Medical Research Compositions and methods for treating kidney disease
WO2005016241A2 (en) 2003-05-16 2005-02-24 Intermune, Inc. Synthetic chemokine receptor ligands and methods of use thereof
PL1680137T3 (pl) 2003-10-14 2013-04-30 F Hoffmann La Roche Ltd Makrocykliczny kwas karboksylowy i związek acylosulfonamidowy jako inhibitor replikacji HCV
US7250298B2 (en) 2004-04-07 2007-07-31 The University Of Chicago Monomeric red fluorescent proteins
US7597884B2 (en) 2004-08-09 2009-10-06 Alios Biopharma, Inc. Hyperglycosylated polypeptide variants and methods of use
WO2007137163A2 (en) 2006-05-19 2007-11-29 Georgia Tech Research Corporation Abc transporter ligand
WO2008008873A2 (en) 2006-07-14 2008-01-17 Georgia Tech Research Corporation Clc channel ligand
US8680235B2 (en) 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
US8679749B2 (en) 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
EP3025727A1 (de) 2008-10-02 2016-06-01 The J. David Gladstone Institutes Verfahren zur behandlung von lebererkrankungen
MX2011007660A (es) 2008-12-18 2011-08-17 Kingdon Craig R Animales transgenicos no humanos que expresan anticuerpos humanizados y usos de los mismos.
PL2482849T3 (pl) 2009-09-30 2018-11-30 Memorial Sloan-Kettering Cancer Center Skojarzona immunoterapia w leczeniu nowotworu
DK2597945T3 (da) 2010-07-26 2020-09-21 Trianni Inc Transgene dyr og fremgangsmåder til anvendelse deraf
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
ES2829376T3 (es) 2013-03-14 2021-05-31 Univ Erasmus Med Ct Rotterdam Mamífero no humano transgénico para la producción de anticuerpos
CA3144958A1 (en) 2019-07-01 2021-01-07 Trianni, Inc. Transgenic mammals and methods of use thereof
JP2022538886A (ja) 2019-07-01 2022-09-06 トリアニ・インコーポレイテッド トランスジェニック哺乳動物およびその使用法
CN111424054B (zh) * 2020-04-21 2021-08-27 鲲石生物科技(深圳)有限公司 改进的大型载体在人类细胞中传递方法的发现及应用
CN114763558B (zh) 2021-01-14 2024-02-20 上海泰槿生物技术有限公司 重组基因组、非人哺乳动物细胞及其产生方法和用途
AU2022268937A1 (en) 2021-05-05 2023-10-26 Trianni, Inc. Transgenic rodents expressing chimeric equine-rodent antibodies and methods of use thereof
CA3235395A1 (en) 2021-11-10 2023-05-19 Peter Burrows Transgenic mammals and methods of use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005083A1 (en) * 1986-12-24 1988-07-14 Whitehead Institute For Biomedical Research Method of inducible gene expression
DE3724482C2 (de) * 1987-07-24 1995-12-14 Boehringer Mannheim Gmbh Verfahren zur Erweiterung der Zellspezifität von eukaryotischen Expressionsvektoren

Also Published As

Publication number Publication date
ES2150411T3 (es) 2000-12-01
GB8904009D0 (en) 1989-04-05
AU5161490A (en) 1990-09-26
EP0962534A3 (de) 1999-12-22
EP0460042B1 (de) 2000-05-03
AU672910B2 (en) 1996-10-17
DK0460042T3 (da) 2000-07-31
EP0962534A2 (de) 1999-12-08
JPH04505552A (ja) 1992-10-01
DE69033531D1 (de) 2000-06-08
ATE192498T1 (de) 2000-05-15
EP0460042A1 (de) 1991-12-11
WO1990010077A1 (en) 1990-09-07
CA2297385A1 (en) 1990-08-23
CA2050920A1 (en) 1990-08-23
AU6872694A (en) 1994-11-17

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee