ES2150411T3 - Vector de integracion de una expresion genica independiente del emplazamiento en las celulas huesped de mamifero. - Google Patents

Vector de integracion de una expresion genica independiente del emplazamiento en las celulas huesped de mamifero.

Info

Publication number
ES2150411T3
ES2150411T3 ES90903867T ES90903867T ES2150411T3 ES 2150411 T3 ES2150411 T3 ES 2150411T3 ES 90903867 T ES90903867 T ES 90903867T ES 90903867 T ES90903867 T ES 90903867T ES 2150411 T3 ES2150411 T3 ES 2150411T3
Authority
ES
Spain
Prior art keywords
gene
site
lambda
downstream
xho
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90903867T
Other languages
English (en)
Inventor
Sarah Jane Flat Woodlan Eccles
Franklin Gerardus Grosveld
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council filed Critical Medical Research Council
Application granted granted Critical
Publication of ES2150411T3 publication Critical patent/ES2150411T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Abstract

UN VECTOR PARA LA INTEGRACION DE UN GEN EN EL MATERIAL GENETICO DE UNA CELULA HUESPED DE MAMIFERO, TAL QUE EL GEN PUEDE SER EXPRESADO POR LA CELULA HUESPED. EL VECTOR CONSTA DE UN PROMOTOR, EL GEN Y UNA REGION CONTROL INMONOGLOBULINA DOMINANTE DERIVADA DEL LOCUS DEL GEN DE LA INMONOGLOBULINA DEL RATON LAMBDA CAPAZ DE EXTRAER UNA CELULA HUESPED TIPO RESTRICTIVO, UNA POSICION DE INTEGRACION INDEPENDIENTE, UN NUMERO DE COPIAS DEPENDIENTE DE LA EXPRESION DE DICHO GEN. LAS POSICIONES SUPER HIPERSENSIBLES DE DNASAI EJEMPLIFICADAS SON I) CERCA DE 2.35 KB MAS ARRIBA DE LA POSICION CAP DEL GEN LAMBDA, REORDENADO II) CERCA DE 2.5 KB MAS ARRIBA DEL SEGMENTO GENOMICO VLAMBDA SUB 2 O III) CERCA DE 30 KB HACIA ABAJO EL GEN LAMBDA, ADAPTADO. LAS CELULAS HUESPED DE MAMIFEROS TRANSFORMADAS CON EL VECTOR SON REVELADAS COMO SON MAMIFEROS TRANSGENICOS TRANSFORMADOS CON EL VECTOR Y UN METODO DE UNA PRODUCCION DE UN POLIPEPTIDO INCLUYENDO UN CULTIVO DE CELULA TRANSFORMADA DE MAMIFERO. UN METODO DE TERAPIA GENETICA INCLUYE LOS PASOS DE I) ELIMINAR CELULAS TRONCO DEL CUERPO DE UN MAMIFERO, II) OPCIONALMENTE MATAR EL RESTO DE LAS CELULAS TRONCO DEL CUERPO, III) TRANSFORMACION DE LAS CELULAS TRONCO EXTRAIDAS CON EL VECTOR CONTENIENDO UN GEN DEFICIENTE O AUSENTE EN EL CUERPO, Y IV) TAMBIEN SE REVELA LA SUSTITUCION DE LAS CELULAS TRONCO TRANSFORMADAS EN EL CUERPO. TAMBIEN ES REVELADO EL ESTIMULADOR FUNCIONAL DE LA INMUNOGLOBULINA LAMBDA, DEL RATON, CONSISTIENDO EN UNA SECUENCIA DE DNA CONSTANDO DE TODO O UNA PARTE FUNCIONAL DE LA SECUENCIA DE DNA ENTRE LA POSICION ECORI 3.8 KB MAS ABAJO DE LA POSICION XHOI DEL GEN REORDENADO Y LA POSICION SNABI 10 KB MAS ABAJO DE ESTA POSICION XHOI. EL ESTIMULADOR FUNCIONAL DE LA INMUNOGLOBULINA DEL RATON PUEDE CONTENER TODO O UNA PARTE DE I) EL 1.3KB HINDIII PRIMERO HACIA EL FRAGMENTO DE DNA HINDII HACIA ABAJO DE LA POSICION ECORI 3.8 KB HACIA ABAJO DE LA POSICION XHOI EN EL GEN REORDENADO DEL RATON, II) EL 3.3 KB HINDIII HACIA EL FRAGMENTO DE DNA HINDIII HACIA ABAJO DE LA POSICION ECORI 3.8 KB HACIA ABAJO DE LA POSICION XHO I EN EL GEN LAMBDA, REORDENADO DEL RATON Y ABARCANDO LA POSICION SNABI 10 KB HACIA ABAJO DE ESTA POSICION XHOI.
ES90903867T 1989-02-22 1990-02-22 Vector de integracion de una expresion genica independiente del emplazamiento en las celulas huesped de mamifero. Expired - Lifetime ES2150411T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898904009A GB8904009D0 (en) 1989-02-22 1989-02-22 Vector

Publications (1)

Publication Number Publication Date
ES2150411T3 true ES2150411T3 (es) 2000-12-01

Family

ID=10652102

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90903867T Expired - Lifetime ES2150411T3 (es) 1989-02-22 1990-02-22 Vector de integracion de una expresion genica independiente del emplazamiento en las celulas huesped de mamifero.

Country Status (10)

Country Link
EP (2) EP0460042B1 (es)
JP (1) JPH04505552A (es)
AT (1) ATE192498T1 (es)
AU (2) AU5161490A (es)
CA (2) CA2297385A1 (es)
DE (1) DE69033531T2 (es)
DK (1) DK0460042T3 (es)
ES (1) ES2150411T3 (es)
GB (1) GB8904009D0 (es)
WO (1) WO1990010077A1 (es)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9027917D0 (en) * 1990-12-21 1991-02-13 Ici Plc Expression systems
DE4228162C1 (de) 1992-08-25 1994-01-13 Rajewsky Klaus Dr Verfahren zum Ersetzen homologer Genabschnitte aus Säugern in der Keimbahn von nicht-menschlichen Säugern
DE19541450C2 (de) * 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Genkonstrukt und dessen Verwendung
TWI250019B (en) 1998-08-18 2006-03-01 Univ California Preventing airway mucus production by administration of EGF-R antagonists
AU783767B2 (en) 1999-10-14 2005-12-01 Takara Bio Usa, Inc. Anthozoa derived chromophores/fluorophores and methods for using the same
JP2003000243A (ja) 2001-06-25 2003-01-07 Takachika Azuma 変異体の製造法
ATE439368T1 (de) 2001-12-19 2009-08-15 Univ Chicago Schnellreifende fluoreszierende proteine und verfahren zu deren anwendung
US7585501B2 (en) 2002-06-14 2009-09-08 Stowers Institute For Medical Research Compositions and methods for treating kidney disease
CA2525647A1 (en) 2003-05-16 2005-02-24 Intermune, Inc. Synthetic chemokine receptor ligands and methods of use thereof
CA2540858C (en) 2003-10-14 2009-12-08 Intermune, Inc. Macrocyclic carboxylic acids and acylsulfonamides as inhibitors of hcv replication
EP1732944B1 (en) 2004-04-07 2012-09-05 The University of Chicago Monomeric red fluorescent proteins
US7597884B2 (en) 2004-08-09 2009-10-06 Alios Biopharma, Inc. Hyperglycosylated polypeptide variants and methods of use
US8106013B2 (en) 2006-05-19 2012-01-31 Georgia Tech Research Corporation ABC transporter ligand GATX1
EP2049138A4 (en) 2006-07-14 2011-11-09 Georgia Tech Res Inst CLC KANALLIGAND
WO2008094316A2 (en) 2006-09-22 2008-08-07 Stowers Institute Of Medical Research Novel branchiostoma derived fluorescent proteins
US8679749B2 (en) 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
EP3025727A1 (en) 2008-10-02 2016-06-01 The J. David Gladstone Institutes Methods of treating liver disease
RU2011129459A (ru) 2008-12-18 2013-01-27 Эрасмус Юниверсити Медикал Сентр Роттердам Трансгенные животные (не человек), экспрессирующие гуманизированные антитела, и их применение
EP2482849B1 (en) 2009-09-30 2018-06-06 Memorial Sloan-Kettering Cancer Center Combination immunotherapy for the treatment of cancer
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
AU2011286185B2 (en) 2010-07-26 2014-08-14 Trianni, Inc. Transgenic animals and methods of use
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
WO2014141189A1 (en) 2013-03-14 2014-09-18 Erasmus University Medical Center Transgenic non-human mammal for antibody production
JP2022538885A (ja) 2019-07-01 2022-09-06 トリアニ・インコーポレイテッド トランスジェニック哺乳動物およびその使用法
WO2021003149A1 (en) 2019-07-01 2021-01-07 Trianni, Inc. Transgenic mammals and methods of use
CN111424054B (zh) * 2020-04-21 2021-08-27 鲲石生物科技(深圳)有限公司 改进的大型载体在人类细胞中传递方法的发现及应用
CN114763558B (zh) 2021-01-14 2024-02-20 上海泰槿生物技术有限公司 重组基因组、非人哺乳动物细胞及其产生方法和用途
CN117440753A (zh) 2021-05-05 2024-01-23 特里安尼公司 表达嵌合马科动物-啮齿动物抗体的转基因啮齿动物及其使用方法
WO2023086815A1 (en) 2021-11-10 2023-05-19 Trianni, Inc. Transgenic mammals and methods of use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005083A1 (en) * 1986-12-24 1988-07-14 Whitehead Institute For Biomedical Research Method of inducible gene expression
DE3724482C2 (de) * 1987-07-24 1995-12-14 Boehringer Mannheim Gmbh Verfahren zur Erweiterung der Zellspezifität von eukaryotischen Expressionsvektoren

Also Published As

Publication number Publication date
DE69033531D1 (de) 2000-06-08
GB8904009D0 (en) 1989-04-05
EP0460042A1 (en) 1991-12-11
DK0460042T3 (da) 2000-07-31
DE69033531T2 (de) 2001-01-11
AU6872694A (en) 1994-11-17
AU5161490A (en) 1990-09-26
CA2297385A1 (en) 1990-08-23
CA2050920A1 (en) 1990-08-23
EP0962534A3 (en) 1999-12-22
ATE192498T1 (de) 2000-05-15
AU672910B2 (en) 1996-10-17
EP0460042B1 (en) 2000-05-03
JPH04505552A (ja) 1992-10-01
WO1990010077A1 (en) 1990-09-07
EP0962534A2 (en) 1999-12-08

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