DE69033531D1 - Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen - Google Patents

Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen

Info

Publication number
DE69033531D1
DE69033531D1 DE69033531T DE69033531T DE69033531D1 DE 69033531 D1 DE69033531 D1 DE 69033531D1 DE 69033531 T DE69033531 T DE 69033531T DE 69033531 T DE69033531 T DE 69033531T DE 69033531 D1 DE69033531 D1 DE 69033531D1
Authority
DE
Germany
Prior art keywords
gene
site
lambda
downstream
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69033531T
Other languages
English (en)
Other versions
DE69033531T2 (de
Inventor
Jane Eccles
Gerardus Grosveld
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council filed Critical Medical Research Council
Publication of DE69033531D1 publication Critical patent/DE69033531D1/de
Application granted granted Critical
Publication of DE69033531T2 publication Critical patent/DE69033531T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Image Analysis (AREA)
  • Devices For Executing Special Programs (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
DE69033531T 1989-02-22 1990-02-22 Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen Expired - Fee Related DE69033531T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898904009A GB8904009D0 (en) 1989-02-22 1989-02-22 Vector
PCT/GB1990/000286 WO1990010077A1 (en) 1989-02-22 1990-02-22 Vector for integration site independent gene expression in mammalian host cells

Publications (2)

Publication Number Publication Date
DE69033531D1 true DE69033531D1 (de) 2000-06-08
DE69033531T2 DE69033531T2 (de) 2001-01-11

Family

ID=10652102

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69033531T Expired - Fee Related DE69033531T2 (de) 1989-02-22 1990-02-22 Vektor zur integrationsstellenunabhängigen genexpression in säugetier-wirtszellen

Country Status (10)

Country Link
EP (2) EP0962534A3 (de)
JP (1) JPH04505552A (de)
AT (1) ATE192498T1 (de)
AU (2) AU5161490A (de)
CA (2) CA2050920A1 (de)
DE (1) DE69033531T2 (de)
DK (1) DK0460042T3 (de)
ES (1) ES2150411T3 (de)
GB (1) GB8904009D0 (de)
WO (1) WO1990010077A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9027917D0 (en) * 1990-12-21 1991-02-13 Ici Plc Expression systems
DE4228162C1 (de) 1992-08-25 1994-01-13 Rajewsky Klaus Dr Verfahren zum Ersetzen homologer Genabschnitte aus Säugern in der Keimbahn von nicht-menschlichen Säugern
DE19541450C2 (de) * 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Genkonstrukt und dessen Verwendung
CA2337422C (en) 1998-08-18 2010-11-02 The Regents Of The University Of California Preventing airway mucus production by administration of egf-r antagonists
EP1305412A2 (de) 1999-10-14 2003-05-02 Clontech Laboratories Inc. Chromo/fluoroproteine aus anthozoa und verwendung der gleichen
JP2003000243A (ja) 2001-06-25 2003-01-07 Takachika Azuma 変異体の製造法
JP4700281B2 (ja) 2001-12-19 2011-06-15 ザ・ユニバーシティ・オブ・シカゴ 急速に成熟する蛍光タンパク質およびその使用法
US7585501B2 (en) 2002-06-14 2009-09-08 Stowers Institute For Medical Research Compositions and methods for treating kidney disease
EP2390262A1 (de) 2003-05-16 2011-11-30 Intermune, Inc. Synthetische Chemokinrezeptorliganden und Verwendungsverfahren dafür
CN102020697B (zh) 2003-10-14 2014-10-29 F·霍夫曼-罗须公司 作为hcv复制抑制剂的巨环羧酸和酰基磺酰胺
EP1732944B1 (de) 2004-04-07 2012-09-05 The University of Chicago Monomere, rotfluoreszierende proteine
US7597884B2 (en) 2004-08-09 2009-10-06 Alios Biopharma, Inc. Hyperglycosylated polypeptide variants and methods of use
WO2007137163A2 (en) 2006-05-19 2007-11-29 Georgia Tech Research Corporation Abc transporter ligand
US8324158B2 (en) 2006-07-14 2012-12-04 Georgia Tech Research Corporation Methods for inhibiting CLC-2 channel with GATX2
WO2008094316A2 (en) 2006-09-22 2008-08-07 Stowers Institute Of Medical Research Novel branchiostoma derived fluorescent proteins
WO2009059305A2 (en) 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
EP2341924A4 (de) 2008-10-02 2013-01-23 David Gladstone Inst Verfahren zur behandlung von hepatitis-c-virusinfektionen
JP5827127B2 (ja) 2008-12-18 2015-12-02 エラスムス・ユニヴァーシティ・メディカル・センター・ロッテルダム ヒト化抗体を発現する非ヒトトランスジェニック動物及びその使用
CN102740887B (zh) 2009-09-30 2015-04-15 斯隆凯特林防癌纪念中心 用于治疗癌症的组合免疫疗法
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
CA2806233C (en) 2010-07-26 2021-12-07 Trianni, Inc. Transgenic animals and methods of use
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
US9980470B2 (en) 2013-03-14 2018-05-29 Erasmus University Medical Center Antibody production
WO2021003149A1 (en) 2019-07-01 2021-01-07 Trianni, Inc. Transgenic mammals and methods of use
KR20220025839A (ko) 2019-07-01 2022-03-03 트리아니, 인코포레이티드 유전자이식 포유동물 및 이의 사용 방법
CN111424054B (zh) * 2020-04-21 2021-08-27 鲲石生物科技(深圳)有限公司 改进的大型载体在人类细胞中传递方法的发现及应用
CN114763558B (zh) 2021-01-14 2024-02-20 上海泰槿生物技术有限公司 重组基因组、非人哺乳动物细胞及其产生方法和用途
CA3216988A1 (en) 2021-05-05 2022-11-10 Werner Mueller Transgenic rodents expressing chimeric equine-rodent antibodies and methods of use thereof
CA3235395A1 (en) 2021-11-10 2023-05-19 Peter Burrows Transgenic mammals and methods of use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005083A1 (en) * 1986-12-24 1988-07-14 Whitehead Institute For Biomedical Research Method of inducible gene expression
DE3724482C2 (de) * 1987-07-24 1995-12-14 Boehringer Mannheim Gmbh Verfahren zur Erweiterung der Zellspezifität von eukaryotischen Expressionsvektoren

Also Published As

Publication number Publication date
ATE192498T1 (de) 2000-05-15
ES2150411T3 (es) 2000-12-01
WO1990010077A1 (en) 1990-09-07
EP0460042A1 (de) 1991-12-11
JPH04505552A (ja) 1992-10-01
AU5161490A (en) 1990-09-26
AU672910B2 (en) 1996-10-17
DK0460042T3 (da) 2000-07-31
EP0962534A3 (de) 1999-12-22
DE69033531T2 (de) 2001-01-11
GB8904009D0 (en) 1989-04-05
AU6872694A (en) 1994-11-17
CA2050920A1 (en) 1990-08-23
CA2297385A1 (en) 1990-08-23
EP0962534A2 (de) 1999-12-08
EP0460042B1 (de) 2000-05-03

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee