DE68927373D1 - Verfahren und mittel zum nachweis von nukleinsäuresequenzen - Google Patents

Verfahren und mittel zum nachweis von nukleinsäuresequenzen

Info

Publication number
DE68927373D1
DE68927373D1 DE68927373T DE68927373T DE68927373D1 DE 68927373 D1 DE68927373 D1 DE 68927373D1 DE 68927373 T DE68927373 T DE 68927373T DE 68927373 T DE68927373 T DE 68927373T DE 68927373 D1 DE68927373 D1 DE 68927373D1
Authority
DE
Germany
Prior art keywords
amplification
nucleic acid
probes
acid sequence
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68927373T
Other languages
English (en)
Other versions
DE68927373T2 (de
Inventor
Rodney Richards
Theodore Jones
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of DE68927373D1 publication Critical patent/DE68927373D1/de
Application granted granted Critical
Publication of DE68927373T2 publication Critical patent/DE68927373T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]
DE68927373T 1988-06-24 1989-06-16 Verfahren und mittel zum nachweis von nukleinsäuresequenzen Expired - Lifetime DE68927373T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22010888A 1988-06-24 1988-06-24
PCT/US1989/002649 WO1989012696A1 (en) 1988-06-24 1989-06-16 Method and reagents for detecting nucleic acid sequences
CA000603916A CA1340160C (en) 1988-06-24 1989-06-26 Method and reagents for detecting nucleic acid sequences

Publications (2)

Publication Number Publication Date
DE68927373D1 true DE68927373D1 (de) 1996-11-28
DE68927373T2 DE68927373T2 (de) 1997-03-20

Family

ID=25672839

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68927373T Expired - Lifetime DE68927373T2 (de) 1988-06-24 1989-06-16 Verfahren und mittel zum nachweis von nukleinsäuresequenzen

Country Status (9)

Country Link
EP (1) EP0379559B1 (de)
JP (2) JP2801051B2 (de)
AT (1) ATE144556T1 (de)
AU (1) AU634969B2 (de)
CA (1) CA1340160C (de)
DE (1) DE68927373T2 (de)
HK (1) HK1006858A1 (de)
NZ (1) NZ229672A (de)
WO (1) WO1989012696A1 (de)

Families Citing this family (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341584C (en) * 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
DE68926504T2 (de) * 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US6004826A (en) * 1988-07-20 1999-12-21 David Segev Repair-mediated process for amplifying and detecting nucleic acid sequences
KR950013953B1 (ko) * 1990-01-26 1995-11-18 애보트 래보라토리즈 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법
EP0453301A3 (en) * 1990-04-20 1993-07-21 Syntex (U.S.A.) Inc. Double receptor polynucleotide assay method
NZ245242A (en) * 1992-01-17 1994-12-22 Pioneer Hi Bred Int Identifying variations between organisms by detecting polymorphisms in single sequence repeats
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US5869252A (en) * 1992-03-31 1999-02-09 Abbott Laboratories Method of multiplex ligase chain reaction
JP3516953B2 (ja) * 1992-08-03 2004-04-05 アボツト・ラボラトリーズ エキソヌクレオリチック活性を用いた標的核酸の検出及び増幅
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
US5681943A (en) * 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
US6406841B1 (en) 1993-07-01 2002-06-18 Abbott Laboratories Methods for the detection of HTLV-II antibodies employing novel HTLV-II NRA envelope peptides
US5571903A (en) * 1993-07-09 1996-11-05 Lynx Therapeutics, Inc. Auto-ligating oligonucleotide compounds
US6113905A (en) * 1995-01-06 2000-09-05 The Regents Of The University Of California Borna disease viral sequences, diagnostics and therapeutics for nervous system diseases
US6015660A (en) * 1995-01-06 2000-01-18 The Regents Of The University Of California Borna disease viral sequences, diagnostics and therapeutics for nervous system diseases
US6077510A (en) * 1995-01-06 2000-06-20 Regents Of The University Of California Borna disease viral sequences, diagnostics and therapeutics for nervous system diseases
DE69921594T2 (de) * 1998-07-24 2005-11-10 Lumigen, Inc., Southfield Methoden zur synthese von polynukleotiden durch ligation von multiplen oligomeren
US5998175A (en) * 1998-07-24 1999-12-07 Lumigen, Inc. Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers
EP1196630B2 (de) 1999-04-20 2018-10-17 Illumina, Inc. Detektion von nukleinsäurereaktionen auf kügelchen-arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
EP1967595A3 (de) 2000-02-16 2008-12-03 Illumina, Inc. Paralleles Genotypisieren von mehreren Patientenproben
WO2002057491A2 (en) 2000-10-24 2002-07-25 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic dna
EP1481076A4 (de) 2001-07-12 2005-05-11 Illumina Inc Multiplex-nukleinsäurereaktionen
US20040259100A1 (en) 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1636337A4 (de) 2003-06-20 2007-07-04 Illumina Inc Verfahren und zusammensetzungen zur ganzgenomischen amplifikation und genotypisierung
JP2007538236A (ja) 2004-05-21 2007-12-27 アトノミックス アクティーゼルスカブ ヒドロゲルを含む表面弾性波センサ
US7981606B2 (en) 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
EP2677039B8 (de) * 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Erkennung von Nukleinsäuretargets mithilfe chemisch reaktiver Oligonnucleotidsonden
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
CN102449169B (zh) 2009-04-01 2016-03-16 德克斯特里蒂诊断公司 化学连接依赖性的探针扩增(clpa)
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2013082164A1 (en) 2011-11-28 2013-06-06 Life Technologies Corporation Enhanced ligation reactions
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
CA2836577C (en) 2011-05-17 2019-06-25 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP2971069B1 (de) 2013-03-13 2018-10-17 Illumina, Inc. Verfahren und systeme zum ausrichten wiederholter dna elemente
ES2887177T3 (es) 2013-03-13 2021-12-22 Illumina Inc Método de preparación de una biblioteca de secuenciación de ácidos nucleicos
EP3080268A1 (de) 2013-12-09 2016-10-19 Illumina, Inc. Verfahren und zusammensetzungen zur gezielten nukleinsäuresequenzierung
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
KR102001554B1 (ko) 2014-01-16 2019-07-18 일루미나, 인코포레이티드 고형 지지체 상에서의 앰플리콘 제조 방법 및 시퀀싱
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
EP3154696B1 (de) 2014-06-10 2020-04-08 DxTerity Diagnostics Incorporated Vorrichtungen und verfahren zur entnahme und stabilisierung biologischer proben
GB201410420D0 (en) 2014-06-11 2014-07-23 Illumina Cambridge Ltd Methods for estimating cluster numbers
WO2015189636A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
CA2953791A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
US20160085910A1 (en) 2014-09-18 2016-03-24 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
WO2016061517A2 (en) 2014-10-17 2016-04-21 Illumina Cambridge Limited Contiguity preserving transposition
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
ES2846730T3 (es) 2015-03-31 2021-07-29 Illumina Cambridge Ltd Concatemerización en superficie de moldes
EP3280821B1 (de) 2015-04-07 2023-12-06 Polyskope Labs Nachweis eines oder mehrerer krankheitserreger
WO2017006108A1 (en) 2015-07-06 2017-01-12 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
DK3377226T3 (da) 2016-03-28 2021-04-26 Illumina Inc Mikrorækker i flere niveauer
WO2018018008A1 (en) 2016-07-22 2018-01-25 Oregon Health & Science University Single cell whole genome libraries and combinatorial indexing methods of making thereof
AU2018259202B2 (en) 2017-04-23 2022-03-24 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US10995369B2 (en) 2017-04-23 2021-05-04 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
US10934584B2 (en) 2017-04-23 2021-03-02 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
US10995104B2 (en) 2017-05-30 2021-05-04 Roche Molecular System, Inc. Catalysts for reversing formaldehyde adducts and crosslinks
EP4293122A3 (de) 2017-06-07 2024-01-24 Oregon Health & Science University Einzelzellen-ganzgenombibliotheken zur methylierungssequenzierung
US20200202977A1 (en) 2017-07-31 2020-06-25 Illumina, Inc. Sequencing system with multiplexed biological sample aggregation
SG11201911871TA (en) 2017-08-01 2020-01-30 Illumina Inc Hydrogel beads for nucleotide sequencing
CN111094589A (zh) 2018-02-13 2020-05-01 伊鲁米纳公司 使用水凝胶珠的dna测序
AU2019248635B2 (en) 2018-04-02 2022-01-27 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
RU2750567C2 (ru) 2018-04-20 2021-06-29 Иллумина, Инк. Способы инкапсулирования одиночных клеток, инкапсулированные клетки и способы их применения
EP4269618A3 (de) 2018-06-04 2024-01-10 Illumina, Inc. Verfahren zur herstellung von einzelzelltranskriptombibliotheken mit hohem durchsatz
US11085036B2 (en) 2018-10-26 2021-08-10 Illumina, Inc. Modulating polymer beads for DNA processing
SG11202102700TA (en) 2018-11-30 2021-04-29 Illumina Inc Analysis of multiple analytes using a single assay
AU2020232618A1 (en) 2019-03-01 2021-04-08 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
BR112021012755A2 (pt) 2019-07-12 2022-04-26 Illumina Cambridge Ltd Composições e métodos para preparar bibliotecas de sequenciamento de ácidos nucleicos usando crispr/cas9 imobilizadas em um suporte sólido
AU2020312787A1 (en) 2019-07-12 2021-06-17 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
CN114008199A (zh) 2019-12-19 2022-02-01 伊路敏纳公司 高通量单细胞文库及其制备和使用方法
BR112022019541A2 (pt) 2020-03-30 2022-11-16 Illumina Inc Métodos e composições para preparação de bibliotecas de ácido nucleico
CN115916967A (zh) 2020-05-12 2023-04-04 伊鲁米纳公司 使用重组末端脱氧核苷酸转移酶生成具有经修饰的碱基的核酸
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
CN115997033A (zh) 2020-06-30 2023-04-21 伊鲁米纳公司 通过合成产生无痕dna的催化控制测序
CA3190588A1 (en) 2020-08-06 2022-02-10 Illumina, Inc. Preparation of rna and dna sequencing libraries using bead-linked transposomes
AU2021329302A1 (en) 2020-08-18 2023-02-16 Illumina Cambridge Limited Sequence-specific targeted transposition and selection and sorting of nucleic acids
WO2022053610A1 (en) 2020-09-11 2022-03-17 Illumina Cambridge Limited Methods of enriching a target sequence from a sequencing library using hairpin adaptors
CA3208854A1 (en) 2021-02-04 2022-08-11 Illumina, Inc. Long indexed-linked read generation on transposome bound beads
WO2022212280A1 (en) 2021-03-29 2022-10-06 Illumina, Inc. Compositions and methods for assessing dna damage in a library and normalizing amplicon size bias
CA3214278A1 (en) 2021-03-29 2022-10-06 Illumina, Inc Improved methods of library preparation
CA3214584A1 (en) 2021-03-30 2022-10-06 Illumina, Inc. Improved methods of isothermal complementary dna and library preparation
BR112023019945A2 (pt) 2021-03-31 2023-11-14 Illumina Cambridge Ltd Métodos para a preparação de bibliotecas de sequenciamento por tagmentação direcional com o uso de tecnologia baseada em transposon com identificadores moleculares exclusivos para correção de erros
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI71768C (fi) * 1984-02-17 1987-02-09 Orion Yhtymae Oy Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning.
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
ZA862334B (en) * 1985-03-28 1987-11-25 Cetus Corp Process for amplifying,detecting and/or cloning nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1339653C (en) * 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
GB8612087D0 (en) * 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
JPS63313658A (ja) * 1987-06-16 1988-12-21 Shibaura Eng Works Co Ltd ロボツト
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
CA1341584C (en) * 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) * 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
CA2046919A1 (en) * 1989-02-14 1990-08-15 Mark P. Pasek Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them

Also Published As

Publication number Publication date
HK1006858A1 (en) 1999-03-19
CA1340160C (en) 1998-12-08
EP0379559B1 (de) 1996-10-23
JPH10309195A (ja) 1998-11-24
DE68927373T2 (de) 1997-03-20
AU3860189A (en) 1990-01-12
JPH03501211A (ja) 1991-03-22
EP0379559A4 (en) 1992-06-03
JP2801051B2 (ja) 1998-09-21
ATE144556T1 (de) 1996-11-15
WO1989012696A1 (en) 1989-12-28
EP0379559A1 (de) 1990-08-01
NZ229672A (en) 1990-12-21
AU634969B2 (en) 1993-03-11

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