DE60330468D1 - Schweine ohne jegliche expression funktioneller alpha-1,3-galactosyltransferase - Google Patents

Schweine ohne jegliche expression funktioneller alpha-1,3-galactosyltransferase

Info

Publication number
DE60330468D1
DE60330468D1 DE60330468T DE60330468T DE60330468D1 DE 60330468 D1 DE60330468 D1 DE 60330468D1 DE 60330468 T DE60330468 T DE 60330468T DE 60330468 T DE60330468 T DE 60330468T DE 60330468 D1 DE60330468 D1 DE 60330468D1
Authority
DE
Germany
Prior art keywords
galactosyltransferase
expression
pigs
functional alpha
alpha
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60330468T
Other languages
English (en)
Inventor
Carol J Phelps
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Revivicor Inc
Original Assignee
Revivicor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Revivicor Inc filed Critical Revivicor Inc
Application granted granted Critical
Publication of DE60330468D1 publication Critical patent/DE60330468D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60330468T 2002-08-21 2003-08-21 Schweine ohne jegliche expression funktioneller alpha-1,3-galactosyltransferase Expired - Lifetime DE60330468D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40477502P 2002-08-21 2002-08-21
PCT/US2003/026622 WO2004028243A2 (en) 2002-08-21 2003-08-21 Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase

Publications (1)

Publication Number Publication Date
DE60330468D1 true DE60330468D1 (de) 2010-01-21

Family

ID=32043169

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60330468T Expired - Lifetime DE60330468D1 (de) 2002-08-21 2003-08-21 Schweine ohne jegliche expression funktioneller alpha-1,3-galactosyltransferase

Country Status (12)

Country Link
US (4) US7795493B2 (de)
EP (3) EP1534819B1 (de)
JP (2) JP2005536228A (de)
AT (1) ATE451448T1 (de)
AU (2) AU2003295322B2 (de)
CA (2) CA2899360A1 (de)
DE (1) DE60330468D1 (de)
DK (2) DK2163614T3 (de)
ES (2) ES2338111T3 (de)
NZ (2) NZ562736A (de)
PT (2) PT2163614T (de)
WO (1) WO2004028243A2 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7795493B2 (en) * 2002-08-21 2010-09-14 Revivicor, Inc. Porcine animals lacking any expression of functional alpha 1, 3 galactosyltransferase
JP2007525955A (ja) 2003-06-06 2007-09-13 ユニバーシティー オブ ピッツバーグ ブタｃｍｐ−ｎ−アセチルノイラミン酸ヒドロキシラーゼ遺伝子
CA2533259C (en) * 2003-07-21 2014-01-28 Lifecell Corporation Acellular tissue matrices made from galactose .alpha.-1,3-galactose-deficient tissue
CA2546853C (en) 2003-11-21 2020-04-21 Revivicor, Inc. Use of interfering rna in the production of transgenic animals
EP2433492B1 (de) 2004-03-17 2018-04-25 Revivicor Inc. Gewebeprodukte von Tieren ohne Expression der funktionalen Alpha-1,3-Galactosyltransferase
US20090043383A1 (en) * 2004-03-29 2009-02-12 Mayo Foundation For Medical Education And Research Genetically modified heart valve xenografts
US20080026457A1 (en) 2004-10-22 2008-01-31 Kevin Wells Ungulates with genetically modified immune systems
KR20070072608A (ko) 2004-10-22 2007-07-04 레비비코르 인코포레이션 유전자 변형된 면역계를 가지는 유제류
WO2007035213A2 (en) 2005-08-09 2007-03-29 Revivicor, Inc. Transgenic ungulates expressing ctla4-ig and uses thereof
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
US8986377B2 (en) 2009-07-21 2015-03-24 Lifecell Corporation Graft materials for surgical breast procedures
WO2011020120A2 (en) 2009-08-14 2011-02-17 Revivicor, Inc. Multi-transgenic pigs for diabetes treatment
FR2951549B1 (fr) * 2009-10-15 2013-08-23 Olivier Schussler Procede d'obtention de bioprotheses medicales implantables
US9420770B2 (en) 2009-12-01 2016-08-23 Indiana University Research & Technology Corporation Methods of modulating thrombocytopenia and modified transgenic pigs
WO2011068778A1 (en) * 2009-12-03 2011-06-09 Lifecell Corporation Nerve treatment devices and methods
BR112012020257A8 (pt) * 2010-02-11 2018-02-14 Recombinetics Inc métodos e aparelhos para produzir artiodátilos transgênicos
CA2827348C (en) 2011-02-14 2021-02-23 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US8980583B2 (en) * 2011-06-30 2015-03-17 Sigma-Aldrich Co. Llc Cells deficient in CMP-N-acetylneuraminic acid hydroxylase and/or glycoprotein alpha-1,3-galactosyltransferase
DK3081189T3 (en) 2012-01-13 2018-10-22 Lifecell Corp BREAST PROSTHESIS AND PROCEDURES FOR MAKING BREAST PROSTHESIS
US9883939B2 (en) 2012-05-08 2018-02-06 The General Hospital Corporation Reducing immunogenicity of xenogeneic transplant tissues
EP3281607B1 (de) 2012-06-21 2019-03-06 LifeCell Corporation Implantierbare prothese mit azellulären gewebebefestigungen
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
US10307510B2 (en) 2013-11-04 2019-06-04 Lifecell Corporation Methods of removing alpha-galactose
WO2016065046A1 (en) 2014-10-22 2016-04-28 Indiana University Research & Technology Corporation Triple transgenic pigs suitable for xenograft
CN116059378A (zh) 2014-12-10 2023-05-05 明尼苏达大学董事会 用于治疗疾病的遗传修饰的细胞、组织和器官
WO2016186803A1 (en) 2015-05-15 2016-11-24 Lifecell Corporation Tissue matrices for plastic surgery
AU2016310471B2 (en) 2015-08-21 2021-05-20 Lifecell Corporation Breast treatment device
EP3506854B1 (de) 2016-08-31 2020-08-19 LifeCell Corporation Brustbehandlungsvorrichtung
US10485167B2 (en) 2016-09-21 2019-11-26 Mtd Products Inc Control assembly for a walk-behind mower
EP3612023A4 (de) 2017-04-20 2021-05-12 Egenesis, Inc. Verfahren zur erzeugung genetisch modifizierter tiere
US11026980B1 (en) 2018-02-26 2021-06-08 Triad Life Sciences, Inc. Flowable birth tissue composition and related methods
US11602548B1 (en) 2018-02-26 2023-03-14 Convatec, Inc Fibrous birth tissue composition and method of use
US10883084B2 (en) 2018-10-05 2021-01-05 Xenotherapeutics, Inc. Personalized cells, tissues, and organs for transplantation from a humanized, bespoke, designated-pathogen free, (non-human) donor and methods and products relating to same
IL281867B2 (en) 2018-10-05 2024-07-01 Xenotherapeutics Inc Products and methods of cell transplantation
JP2022527061A (ja) 2019-03-25 2022-05-30 ゼノセラピューティクス インコーポレイテッド ヒト化された、オーダーメイドされた、指定された病原体を含まない（非ヒト）ドナーから移植するための、個別化された細胞、組織、及び臓器、ならびにそれらに関連する方法及び製品
US11298220B2 (en) 2019-05-03 2022-04-12 Lifecell Corporation Breast treatment device
US20220409669A1 (en) * 2019-12-02 2022-12-29 The General Hospital Corporation Nerve Xenografts and Related Methods
CN116615448A (zh) 2020-08-24 2023-08-18 药物治疗股份有限公司 免疫相容性细胞、组织、器官以及用于在最大程度地减少附带基因组破坏的情况下进行针对沉默、人源化和个性化的移植的方法
JP2023550257A (ja) 2020-10-21 2023-12-01 ティシュー テスティング テクノロジーズ エルエルシー 脱細胞化組織の免疫原性の最小限化
JP2023551404A (ja) 2020-11-20 2023-12-08 レビビコア， インコーポレイテッド 異種間移植のための成長ホルモン受容体ノックアウトを有する多重トランスジェニックブタ
WO2022256401A1 (en) * 2021-06-01 2022-12-08 Xenotherapeutics, Inc. Xenogeneic nerve transplants and methods
US20230255185A1 (en) 2021-09-20 2023-08-17 Revivicor, Inc. Multitransgenic pigs comprising ten genetic modifications for xenotransplantation
US20240294869A1 (en) 2023-03-03 2024-09-05 Nugift Medical Llc Pig xenotransplants into humans without chronic immunosuppression

Family Cites Families (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) * 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4863852A (en) * 1985-07-03 1989-09-05 Virginia Tech Intellectual Properties, Inc. Method of detecting, isolating and purifying clostridium difficile toxin A and its receptors
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5354768A (en) * 1988-07-26 1994-10-11 Sankyo Company, Limited Use of imidazopyrazole derivatives as analgesics and anti-inflammatory agents
BR8907666A (pt) 1988-09-21 1991-07-30 Cambridge Animal Biotech Derivacao de linhas de celulas embrionarias pluripotenciais de animais domesticos
US4944384A (en) 1989-01-30 1990-07-31 Hay & Forage Industries Trash discharge apparatus for crop transferring conveyor mechanism
US5175383A (en) * 1989-02-17 1992-12-29 President And Fellows Of Harvard College Animal model for benign prostatic disease
US4997384A (en) 1989-04-17 1991-03-05 Otis Engineering Corporation Wet connector
US5324663A (en) 1990-02-14 1994-06-28 The Regents Of The University Of Michigan Methods and products for the synthesis of oligosaccharide structures on glycoproteins, glycolipids, or as free molecules, and for the isolation of cloned genetic sequences that determine these structures
WO1991018088A1 (en) * 1990-05-23 1991-11-28 The United States Of America, Represented By The Secretary, United States Department Of Commerce Adeno-associated virus (aav)-based eucaryotic vectors
US5173414A (en) * 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
US5589582A (en) 1992-10-27 1996-12-31 Biotransplant, Inc. Polynucleotides en coding porcine cytokines
EP0706319A4 (de) 1993-01-20 1998-04-22 Biotransplant Inc Retrovirale vektoren mit der fähigkeit zur expression multimerer proteine von mehreren translationsinitiations-orten
US5496720A (en) 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
JPH06253856A (ja) 1993-02-26 1994-09-13 Amano Pharmaceut Co Ltd ムタロターゼ遺伝子
CA2157659C (en) 1993-03-16 2003-10-21 Mauro S. Sandrin Use of porcine gal .alpha. (1,3) galactosyl transferase in xenograft therapies
US6331658B1 (en) * 1993-04-20 2001-12-18 Integris Baptist Medical Center, Inc. Genetically engineered mammals for use as organ donors
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
WO1995020661A1 (en) * 1994-01-27 1995-08-03 Bresatec Ltd. Materials and methods for management of hyperacute rejection in human xenotransplantation
US5849991A (en) * 1994-01-27 1998-12-15 Bresatch Limited Mice homozygous for an inactivated α 1,3-galactosyl transferase gene
JPH10504442A (ja) * 1994-04-13 1998-05-06 バイオトランスプラント，インコーポレイテッド α（１，３）ガラクトシルトランスフェラーゼ陰性ブタ
AUPM516994A0 (en) 1994-04-20 1994-05-12 Gene Shears Pty. Limited An in vivo gene expression system
US5714353A (en) * 1994-05-24 1998-02-03 Research Corporation Technologies, Inc. Safe vectors for gene therapy
US5989808A (en) 1994-06-14 1999-11-23 American Cyanamid Company Identification of compounds affecting specific interaction of peptide binding pairs
EP0769902A4 (de) 1994-06-15 2001-02-28 Alexion Pharma Inc Verfahren zur verringerung der hyperakuten abstossung von xenotransplantaten
US5850004A (en) * 1994-08-02 1998-12-15 Cornell Research Foundation, Inc. Transgenic mouse deficient in inducible nitric oxide synthase
WO1996006165A1 (en) 1994-08-19 1996-02-29 The General Hospital Corporation Genetically engineered swine cells
PT787200E (pt) 1994-10-28 2005-08-31 Univ Pennsylvania Adenovirus melhorado e metodos para a sua utilizacao
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5922601A (en) * 1995-01-19 1999-07-13 Biotransplant, Inc. High efficiency gene trap selection of regulated genetic loci
US5958713A (en) 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
WO1996028967A1 (fr) 1995-03-17 1996-09-26 Chihiro Koike Mammiferes transgeniques autres que des primates, ou des serotypes de primates superieurs ont ete exprimes par un transfert de genes etrangers et procede pour creer ceux-ci
US6610288B1 (en) 1995-05-26 2003-08-26 Diacrin, Inc. Porcine hepatocytes for use in treatment of disorders characterized by insufficient liver function
US5962644A (en) 1995-06-07 1999-10-05 Biotransplant, Inc. Porcine CD34
DE69604298T2 (de) 1995-09-22 2000-05-18 Bioimage A/S, Soeborg Varianten des grünen fluoreszenzproteins, gfp
US5952236A (en) 1995-10-26 1999-09-14 Thompson; Richard B. Enzyme-based fluorescence biosensor for chemical analysis
US5856106A (en) 1995-11-01 1999-01-05 Biotransplant, Inc. Determination of antibody production against administered therapeutic glycoproteins, especially monoclonal antibodies
IL124293A0 (en) 1995-11-03 1998-12-06 Mount Sinai Medical Center Method and compositions for the reduction of xenotransplantation rejection
US5968738A (en) 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US6020192A (en) 1996-01-18 2000-02-01 University Of Florida Humanized green fluorescent protein genes and methods
US6255558B1 (en) 1996-02-14 2001-07-03 Btg International Limited Gene expression
US6027881A (en) 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5948889A (en) 1996-05-21 1999-09-07 Case Western Reserve University Compositions and methods for screening antimicrobials
CA2233705A1 (en) * 1996-08-02 1998-02-12 The Austin Research Institute Improved nucleic acids encoding a chimeric glycosyltransferase
US5948681A (en) 1996-08-14 1999-09-07 Children's Hospital Of Philadelphia Non-viral vehicles for use in gene transfer
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
DK0959899T3 (da) 1996-08-16 2009-09-07 Biotransplant Inc LO-CD2a-antistof samt dets anvendelse til h mning af T-celleaktivering og -proliferation
JP2001500725A (ja) 1996-08-19 2001-01-23 ユニヴァーシティー オヴ マサチューセッツ 種間核移植により製造される胚性または幹細胞様細胞株
US5914233A (en) 1996-08-23 1999-06-22 Osteo Screen Screening assay for the identification of agents which alter expression of PTH-rP
AUPO182396A0 (en) * 1996-08-23 1996-09-12 Austin Research Institute, The Improved nucleic acids for reducing carbohydrate epitopes
WO1998008839A1 (en) 1996-08-26 1998-03-05 Eli Lilly And Company Combinatorial process for preparing substituted thiophene libraries
AU722375B2 (en) 1996-09-06 2000-08-03 Trustees Of The University Of Pennsylvania, The Methods using cre-lox for production of recombinant adeno-associated viruses
US6025192A (en) 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
US6255071B1 (en) 1996-09-20 2001-07-03 Cold Spring Harbor Laboratory Mammalian viral vectors and their uses
US6136566A (en) 1996-10-04 2000-10-24 Lexicon Graphics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
TW371617B (en) 1996-10-09 1999-10-11 Of Animal And Plant Health Inspection And Quarantine Council Of Agriculture Executive Yuan Bureau Method to transplant GFP into autographa californica multiple nuclear polyhedrosis virus for inflicting pest in an attempt to detect and flow up it existence and to improve life span against UV
US6455037B1 (en) * 1996-11-01 2002-09-24 Mount Sinai School Of Medicine Of The City University Of New York Cells expressing an αgala nucleic acid and methods of xenotransplantation
EP0943004A2 (de) 1996-11-18 1999-09-22 McGill University Post-mitotische neuronen die adenovirusvektoren enthalten die apoptosis und wachstum modulieren
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
US5994077A (en) 1997-01-31 1999-11-30 The Board Of Trustees Of The Leland Stanford Junior University Flourescence-based isolation of differentially induced genes
JP2001518074A (ja) 1997-02-05 2001-10-09 バイオトランスプラント インク． Ｂ細胞の免疫寛容誘導
WO2000006194A2 (en) 1997-02-05 2000-02-10 Biotransplant, Inc. Depletion of cells responsible for antibody-mediated graft rejection
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5994071A (en) 1997-04-04 1999-11-30 Albany Medical College Assessment of prostate cancer
US6251384B1 (en) 1997-04-28 2001-06-26 Anticancer, Inc. Metastasis models using green fluorescent protein (GFP) as a marker
US5993778A (en) 1997-05-07 1999-11-30 Firestein; Stuart J. Functional expression of, and assay for, functional cellular receptors in vivo
US6251582B1 (en) 1997-07-17 2001-06-26 New York University Alternative G-coupled receptors associated with retroviral entry into cells, methods of identifying the same, and diagnostic and therapeutic uses thereof
WO1999009163A1 (en) 1997-08-13 1999-02-25 Biotransplant, Inc. Porcine stem cell factor varients and recombinant cells expressing such polypeptides
AU8827998A (en) 1997-08-14 1999-03-08 Biotransplant Incorporated Porcine totipotent cells and method for long-term culture
US6251677B1 (en) 1997-08-25 2001-06-26 The Trustees Of The University Of Pennsylvania Hybrid adenovirus-AAV virus and methods of use thereof
US6153409A (en) 1997-09-11 2000-11-28 University Of Maryland Biotechnology Institute Process for continuous optimized protein production in insect larvae
CA2304642C (en) 1997-09-26 2014-11-18 Athersys, Inc. Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna
US6482937B1 (en) 1997-10-09 2002-11-19 Biotransplant, Inc. Porcine Oct-4 promoter
AU1581899A (en) 1997-10-28 1999-05-17 Biotransplant Incorporated Nuclear transfer for production of transgenic animal embryo
US5968773A (en) 1997-11-14 1999-10-19 Heddle; John A. System and method for regulation of gene expression
US6013447A (en) 1997-11-21 2000-01-11 Innovir Laboratories, Inc. Random intracellular method for obtaining optimally active nucleic acid molecules
US5922576A (en) 1998-02-27 1999-07-13 The John Hopkins University Simplified system for generating recombinant adenoviruses
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
WO1999046592A1 (en) 1998-03-09 1999-09-16 The Regents Of The University Of California Regulation of the cell cycle by sterols
CA2513336A1 (en) 1998-03-20 1999-09-30 Benitec Australia Ltd. Control of gene expression in a non-human eukaryotic cell, tissue or organ
US6080576A (en) 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6037133A (en) 1998-04-17 2000-03-14 Clontech Laboratories, Inc. IκBEGFP constructs, cell lines and methods of use
US6316181B1 (en) 1998-04-24 2001-11-13 Virginia Commonwealth University Establishment of cell lines with persistent expression of a green fluorescent protein (GFP) using a pIRES/EGFP DNA vector construct
US6150176A (en) 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
AU5575899A (en) 1998-08-20 2000-03-14 Biotransplant Incorporated Inbred mhc-homozygous miniature swine and uses thereof
US6180343B1 (en) 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US5985577A (en) 1998-10-14 1999-11-16 The Trustees Of Columbia University In The City Of New York Protein conjugates containing multimers of green fluorescent protein
US6203986B1 (en) 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
US6268201B1 (en) 1998-10-23 2001-07-31 Albert Einstein College Of Medicine Of Yeshiva University IniB, iniA and iniC genes of mycobacteria and methods of use
US6270958B1 (en) 1998-10-29 2001-08-07 Washington University Detection of negative-strand RNA viruses
US6700037B2 (en) 1998-11-24 2004-03-02 Infigen, Inc. Method of cloning porcine animals
US6258998B1 (en) * 1998-11-24 2001-07-10 Infigen, Inc. Method of cloning porcine animals
US6210922B1 (en) 1998-11-30 2001-04-03 National Research Council Of Canada Serum free production of recombinant proteins and adenoviral vectors
DK1159415T3 (da) 1999-03-04 2010-05-03 Revivicor Inc Genetisk modifikation af somatiske celler og anvendelse deraf
AU7744800A (en) 1999-09-30 2001-04-30 Alexion Pharmaceuticals, Inc. Compositions and methods for altering gene expression
AU1099701A (en) * 1999-10-22 2001-05-08 University Of Pittsburgh Alpha1-3 galactosyltransferase gene and promoter
WO2002074935A2 (en) 2001-03-21 2002-09-26 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals
AU2002242538B2 (en) * 2001-03-21 2007-06-28 Geron Corporation Animal tissue with carbohydrate antigens compatible for human transplantation
US7126039B2 (en) * 2001-03-21 2006-10-24 Geron Corporation Animal tissue with carbohydrate antigens compatible for human transplantation
RU2243630C2 (ru) 2001-08-06 2004-12-27 Жаров Владимир Павлович Оптическое устройство для пространственной манипуляции объектами
WO2003055302A1 (en) 2001-12-21 2003-07-10 The Curators Of The University Of Missouri Knockout swine and methods for making the same
JP2005535343A (ja) 2002-08-14 2005-11-24 イマージ バイオセラピューティクス，インコーポレーテッド α（１，３）−ガラクトシルトランスフェラーゼ欠損細胞、選択する方法、およびそれらから作製されたα（１，３）−ガラクトシルトランスフェラーゼ欠損ブタ
US7795493B2 (en) * 2002-08-21 2010-09-14 Revivicor, Inc. Porcine animals lacking any expression of functional alpha 1, 3 galactosyltransferase

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