DE60309476T2 - Optische vorrichtung und verfahren zur messung von lichttransmission - Google Patents
Optische vorrichtung und verfahren zur messung von lichttransmission Download PDFInfo
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- DE60309476T2 DE60309476T2 DE60309476T DE60309476T DE60309476T2 DE 60309476 T2 DE60309476 T2 DE 60309476T2 DE 60309476 T DE60309476 T DE 60309476T DE 60309476 T DE60309476 T DE 60309476T DE 60309476 T2 DE60309476 T2 DE 60309476T2
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N2013/003—Diffusion; diffusivity between liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/466—Flow patterns using more than one column with separation columns in parallel
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Light Receiving Elements (AREA)
- Arrangements For Transmission Of Measured Signals (AREA)
- Light Guides In General And Applications Therefor (AREA)
- Geophysics And Detection Of Objects (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0219248 | 2002-08-17 | ||
| GBGB0219248.2A GB0219248D0 (en) | 2002-08-17 | 2002-08-17 | OPtical assembly and method for detection of light transmission |
| PCT/GB2003/003591 WO2004017061A1 (en) | 2002-08-17 | 2003-08-15 | Optical assembly and method for detection of light transmission |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60309476D1 DE60309476D1 (de) | 2006-12-14 |
| DE60309476T2 true DE60309476T2 (de) | 2007-08-16 |
Family
ID=9942547
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60309476T Expired - Lifetime DE60309476T2 (de) | 2002-08-17 | 2003-08-15 | Optische vorrichtung und verfahren zur messung von lichttransmission |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7262847B2 (https=) |
| EP (1) | EP1530716B1 (https=) |
| JP (1) | JP4411457B2 (https=) |
| CN (1) | CN1688878B (https=) |
| AT (1) | ATE344452T1 (https=) |
| AU (1) | AU2003259329A1 (https=) |
| DE (1) | DE60309476T2 (https=) |
| GB (1) | GB0219248D0 (https=) |
| WO (1) | WO2004017061A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102014220565B4 (de) * | 2013-10-11 | 2020-09-10 | Waters Technologies Corporation | Phasendetektion in Mehrphasen-Fluiden |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8614768B2 (en) | 2002-03-18 | 2013-12-24 | Raytheon Company | Miniaturized imaging device including GRIN lens optically coupled to SSID |
| US20060199946A1 (en) * | 2003-04-21 | 2006-09-07 | Hideaki Shimada | Apoptosis inducing agent and method for inducing apoptosis |
| WO2006078366A2 (en) * | 2005-01-18 | 2006-07-27 | Solus Biosystems, Inc. | Multiple sample screening using ir spectroscopy with capillary isoelectric focusing |
| US8374887B1 (en) | 2005-02-11 | 2013-02-12 | Emily H. Alexander | System and method for remotely supervising and verifying pharmacy functions |
| JP4645255B2 (ja) * | 2005-03-24 | 2011-03-09 | 株式会社島津製作所 | 分析試料調製用溶媒 |
| CN101175986B (zh) * | 2005-04-06 | 2010-10-13 | 康宁股份有限公司 | 玻璃检测系统及其使用方法 |
| US20070131870A1 (en) * | 2005-12-12 | 2007-06-14 | Combisep | Multiplexed CE fluorescence system |
| US8021531B2 (en) * | 2006-04-14 | 2011-09-20 | Caliper Life Sciences, Inc. | Method for modifying the concentration of reactants in a microfluidic device |
| US7504834B2 (en) * | 2006-12-20 | 2009-03-17 | 3M Innovative Properties Company | Detection system |
| US7835074B2 (en) | 2007-06-05 | 2010-11-16 | Sterling Lc | Mini-scope for multi-directional imaging |
| WO2009048833A1 (en) * | 2007-10-09 | 2009-04-16 | Siemens Healthcare Diagnostics Inc. | Two dimensional imaging of reacted areas on a reagent |
| US7969659B2 (en) | 2008-01-11 | 2011-06-28 | Sterling Lc | Grin lens microscope system |
| EP2299894B1 (en) | 2008-06-18 | 2020-09-02 | Sarcos LC | Transparent endoscope head defining a focal length |
| EP2315015A4 (en) * | 2008-07-22 | 2012-02-08 | Arkray Inc | DEVICE FOR ANALYSIS BY A CAPILLARY ELECTROPHORESIS METHOD |
| WO2010014792A2 (en) | 2008-07-30 | 2010-02-04 | Sterling Lc | Method and device for incremental wavelength variation to analyze tissue |
| US9060704B2 (en) | 2008-11-04 | 2015-06-23 | Sarcos Lc | Method and device for wavelength shifted imaging |
| WO2010068670A1 (en) | 2008-12-09 | 2010-06-17 | Advanced Analytical Technologies, Inc. | Capillary electrophoresis fluorescent detection system |
| US8368882B2 (en) * | 2009-01-30 | 2013-02-05 | Gen-Probe Incorporated | Systems and methods for detecting a signal and applying thermal energy to a signal transmission element |
| JP5219879B2 (ja) * | 2009-02-20 | 2013-06-26 | 倉敷紡績株式会社 | logPの決定方法、および該方法に使用される二相系溶媒 |
| GB0903992D0 (en) | 2009-03-09 | 2009-04-22 | Paraytec Ltd | Optical cell assenbly, cartridge and apparatus |
| JP2012525591A (ja) * | 2009-04-27 | 2012-10-22 | プロテイン・デイスカバリー・インコーポレーテツド | プログラム可能な電気泳動ノッチフィルターシステムと方法 |
| WO2011041730A2 (en) | 2009-10-01 | 2011-04-07 | Jacobsen Stephen C | Light diffusion apparatus |
| US9661996B2 (en) | 2009-10-01 | 2017-05-30 | Sarcos Lc | Needle delivered imaging device |
| US9144664B2 (en) | 2009-10-01 | 2015-09-29 | Sarcos Lc | Method and apparatus for manipulating movement of a micro-catheter |
| US8828028B2 (en) | 2009-11-03 | 2014-09-09 | Raytheon Company | Suture device and method for closing a planar opening |
| US9930297B2 (en) | 2010-04-30 | 2018-03-27 | Becton, Dickinson And Company | System and method for acquiring images of medication preparations |
| GB201016992D0 (en) | 2010-10-08 | 2010-11-24 | Paraytec Ltd | Viscosity measurement apparatus and method |
| AT510765B1 (de) * | 2010-12-15 | 2012-09-15 | Wolfgang Dipl Ing Vogl | Vorrichtung zur photometrischen bzw. spektrometrischen untersuchung einer flüssigen probe |
| US9140666B2 (en) | 2012-03-15 | 2015-09-22 | Advanced Analytical Technologies, Inc. | Capillary electrophoresis system |
| KR101441359B1 (ko) * | 2012-01-16 | 2014-09-23 | 코닝정밀소재 주식회사 | 광전지용 커버유리의 투과율 측정장치 |
| US11340191B2 (en) | 2012-03-15 | 2022-05-24 | Agilent Technologies, Inc. | UV-absorbance multichannel capillary electrophoresis system |
| US11016057B2 (en) | 2012-03-15 | 2021-05-25 | Agilent Technologies, Inc. | Pulse-field multiplex capillary electrophoresis system |
| US9090666B2 (en) * | 2012-06-27 | 2015-07-28 | Tiansong Wang | Lensed optical fiber for illuminating capillary tube |
| WO2015009970A1 (en) * | 2013-07-18 | 2015-01-22 | Erythron Llc | Spectroscopic measurements with parallel array detector |
| US20180021773A1 (en) * | 2014-07-25 | 2018-01-25 | Loughborough University | Assay devices, methods for carrying out assays, assay kits and method for manufacturing assay devices |
| US10679342B2 (en) | 2014-09-08 | 2020-06-09 | Becton, Dickinson And Company | Aerodynamically streamlined enclosure for input devices of a medication preparation system |
| EP3073389A1 (en) | 2015-03-24 | 2016-09-28 | Malvern Instruments Limited | Multicomponent model parameterisation |
| GB2544507B (en) * | 2015-11-18 | 2017-11-22 | Servomex Group Ltd | Method and system for reduction of influence of baseline distortion in absorption spectroscopy measurements |
| CN114002196B (zh) * | 2016-02-22 | 2025-03-28 | 株式会社日立高新技术 | 发光检测装置 |
| DE112016007594B3 (de) * | 2016-02-22 | 2021-06-10 | Hitachi High-Tech Corporation | Lichtemittierende detektionsvorrichtung |
| WO2017192763A1 (en) * | 2016-05-03 | 2017-11-09 | Niagara Bottling, Llc | Systems and methods for optical measurement of container wall thickness |
| CN106153549A (zh) * | 2016-06-14 | 2016-11-23 | 暨南大学 | 基于分子吸收的毛细管电泳检测器及检测方法 |
| JP6786039B2 (ja) * | 2017-03-03 | 2020-11-18 | 国立大学法人 熊本大学 | 光学測定システム、光学セル及び光学測定方法 |
| EP3444603B1 (en) * | 2017-08-15 | 2024-08-07 | Agilent Technologies, Inc. | Improved uv-absorbance multichannel capillary electrophoresis system |
| JP6731901B2 (ja) * | 2017-09-29 | 2020-07-29 | 株式会社日立ハイテク | 分析装置 |
| JP6933551B2 (ja) * | 2017-10-23 | 2021-09-08 | アークレイ株式会社 | 判定方法、分析方法及び分析システム |
| CN112262308B (zh) | 2018-06-22 | 2024-10-29 | 株式会社日立高新技术 | 电泳装置 |
| JP7228060B2 (ja) * | 2018-06-22 | 2023-02-22 | 株式会社日立ハイテク | 電気泳動装置 |
| CN109900973B (zh) * | 2019-02-25 | 2021-06-01 | 西安电子科技大学 | 基于规则矩形折射率单元的带有光强衰减的射线追踪方法 |
| CN112087527B (zh) * | 2020-09-22 | 2024-01-09 | 中国科学技术大学 | 一种远程自动化相机测试平台和方法 |
| GB2616553B (en) * | 2020-12-18 | 2025-06-18 | Hitachi High Tech Corp | Capillary array electrophoresis device |
| JP7495999B2 (ja) * | 2020-12-18 | 2024-06-05 | 株式会社日立ハイテク | キャピラリアレイ |
| CN116569023A (zh) * | 2020-12-18 | 2023-08-08 | 株式会社日立高新技术 | 毛细管阵列电泳装置 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5239360A (en) * | 1988-10-21 | 1993-08-24 | Applied Biosystems, Inc. | Lens for capillary electrophoresis and chromatography |
| JPH07209251A (ja) * | 1994-01-14 | 1995-08-11 | Hitachi Ltd | 電気泳動装置 |
| US5312535A (en) * | 1992-07-17 | 1994-05-17 | Beckman Instruments, Inc. | Capillary electrophoresis detection |
| US5274227A (en) * | 1992-10-23 | 1993-12-28 | Applied Biosystems, Inc. | Capillary detector cell having imaging elements positioned to optimize sensitivity |
| DE69323060T2 (de) * | 1993-03-18 | 1999-06-10 | Novartis Ag, Basel | Optische Detektorvorrichtung für die chemische Analyse von kleinen fluiden Probenvolumina |
| JP3563140B2 (ja) * | 1995-01-19 | 2004-09-08 | 株式会社日立製作所 | キャピラリーアレイ電気泳動装置 |
| US5582705A (en) * | 1995-05-19 | 1996-12-10 | Iowa State University Research Foundation, Inc. | Multiplexed capillary electrophoresis system |
| US5694215A (en) * | 1996-03-04 | 1997-12-02 | Carver; David R. | Optical array and processing electronics and method therefor for use in spectroscopy |
| JP3559648B2 (ja) * | 1996-04-23 | 2004-09-02 | 株式会社日立製作所 | キャピラリーアレー電気泳動装置 |
| AU6382800A (en) | 1999-09-09 | 2001-04-10 | Iowa State University Research Foundation Inc. | Method of analyzing multiple samples simultaneously by detecting absorption and systems for use in such a method |
-
2002
- 2002-08-17 GB GBGB0219248.2A patent/GB0219248D0/en not_active Ceased
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2003
- 2003-08-15 JP JP2004528686A patent/JP4411457B2/ja not_active Expired - Fee Related
- 2003-08-15 DE DE60309476T patent/DE60309476T2/de not_active Expired - Lifetime
- 2003-08-15 AT AT03787908T patent/ATE344452T1/de not_active IP Right Cessation
- 2003-08-15 AU AU2003259329A patent/AU2003259329A1/en not_active Abandoned
- 2003-08-15 EP EP03787908A patent/EP1530716B1/en not_active Expired - Lifetime
- 2003-08-15 US US10/524,779 patent/US7262847B2/en not_active Expired - Lifetime
- 2003-08-15 WO PCT/GB2003/003591 patent/WO2004017061A1/en not_active Ceased
- 2003-08-15 CN CN038242087A patent/CN1688878B/zh not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102014220565B4 (de) * | 2013-10-11 | 2020-09-10 | Waters Technologies Corporation | Phasendetektion in Mehrphasen-Fluiden |
Also Published As
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|---|---|
| EP1530716B1 (en) | 2006-11-02 |
| US7262847B2 (en) | 2007-08-28 |
| ATE344452T1 (de) | 2006-11-15 |
| JP4411457B2 (ja) | 2010-02-10 |
| US20050231718A1 (en) | 2005-10-20 |
| AU2003259329A1 (en) | 2004-03-03 |
| WO2004017061A1 (en) | 2004-02-26 |
| DE60309476D1 (de) | 2006-12-14 |
| EP1530716A1 (en) | 2005-05-18 |
| HK1076151A1 (en) | 2006-01-06 |
| JP2005535895A (ja) | 2005-11-24 |
| CN1688878B (zh) | 2011-11-16 |
| CN1688878A (zh) | 2005-10-26 |
| GB0219248D0 (en) | 2002-09-25 |
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