DE60222857T2 - Concatemere unterschiedlich exprimierter multipler gene - Google Patents
Concatemere unterschiedlich exprimierter multipler gene Download PDFInfo
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- DE60222857T2 DE60222857T2 DE60222857T DE60222857T DE60222857T2 DE 60222857 T2 DE60222857 T2 DE 60222857T2 DE 60222857 T DE60222857 T DE 60222857T DE 60222857 T DE60222857 T DE 60222857T DE 60222857 T2 DE60222857 T2 DE 60222857T2
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N2800/00—Nucleic acids vectors
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- C12N2800/00—Nucleic acids vectors
- C12N2800/20—Pseudochromosomes, minichrosomosomes
- C12N2800/206—Pseudochromosomes, minichrosomosomes of yeast origin, e.g. YAC, 2u
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Applications Claiming Priority (5)
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| DK200100127 | 2001-01-25 | ||
| DKPA200100127 | 2001-01-25 | ||
| US30102201P | 2001-06-27 | 2001-06-27 | |
| US301022P | 2001-06-27 | ||
| PCT/DK2002/000055 WO2002059296A2 (en) | 2001-01-25 | 2002-01-25 | Concatemers of differentially expressed multiple genes |
Publications (2)
| Publication Number | Publication Date |
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| DE60222857D1 DE60222857D1 (de) | 2007-11-22 |
| DE60222857T2 true DE60222857T2 (de) | 2008-07-17 |
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| DE60222857T Expired - Lifetime DE60222857T2 (de) | 2001-01-25 | 2002-01-25 | Concatemere unterschiedlich exprimierter multipler gene |
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| DE (1) | DE60222857T2 (https=) |
| NZ (1) | NZ527208A (https=) |
| WO (1) | WO2002059296A2 (https=) |
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| US7119250B2 (en) | 1997-06-03 | 2006-10-10 | The University Of Chicago | Plant centromere compositions |
| US7227057B2 (en) | 1997-06-03 | 2007-06-05 | Chromatin, Inc. | Plant centromere compositions |
| US7193128B2 (en) | 1997-06-03 | 2007-03-20 | Chromatin, Inc. | Methods for generating or increasing revenues from crops |
| US7235716B2 (en) | 1997-06-03 | 2007-06-26 | Chromatin, Inc. | Plant centromere compositions |
| US8008459B2 (en) | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
| WO2002059297A2 (en) | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | A library of a collection of cells |
| WO2004016791A1 (en) * | 2002-08-01 | 2004-02-26 | Evolva Ltd | Methods of mixing large numbers of heterologous genes |
| CA2958259C (en) | 2004-10-22 | 2020-06-30 | Revivicor, Inc. | Ungulates with genetically modified immune systems |
| DK2348827T3 (en) | 2008-10-27 | 2015-07-20 | Revivicor Inc | IMMUNICIPLY COMPROMATED PETS |
| US9096909B2 (en) | 2009-07-23 | 2015-08-04 | Chromatin, Inc. | Sorghum centromere sequences and minichromosomes |
| WO2011146833A1 (en) | 2010-05-20 | 2011-11-24 | Evolva Inc. | Method of producing isoprenoid compounds in yeast |
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| JP6295512B2 (ja) * | 2012-03-15 | 2018-03-20 | 株式会社豊田中央研究所 | 酵母における外来遺伝子の発現産物の生産方法、酵母における発現調節剤及びその利用 |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| CN106591370A (zh) * | 2015-10-19 | 2017-04-26 | 南京华贞生物医药科技有限公司 | 一种治疗自身免疫性相关疾病以及糖尿病的病毒载体及其构建方法和应用 |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12595501B2 (en) * | 2019-06-13 | 2026-04-07 | Global Life Sciences Solutions Usa Llc | Expression of products from nucleic acid concatemers |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| CN116312618B (zh) * | 2023-03-27 | 2025-09-16 | 中科南京智能技术研究院 | 一种说话人识别方法及系统 |
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| US5316922A (en) * | 1992-04-15 | 1994-05-31 | The United States Of America As Represented By Department Of Health And Human Services | Method for indentifying and expressing proteins that recognize and adhere to specific probes |
| US6242211B1 (en) * | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
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| EP1360308A2 (en) | 2003-11-12 |
| CA2474161C (en) | 2012-03-27 |
| JP5001346B2 (ja) | 2012-08-15 |
| JP2010057519A (ja) | 2010-03-18 |
| WO2002059296A8 (en) | 2004-04-15 |
| CA2474161A1 (en) | 2002-08-01 |
| ATE375394T1 (de) | 2007-10-15 |
| JP2004525623A (ja) | 2004-08-26 |
| AU2002227882B2 (en) | 2007-06-28 |
| AU2002227882C9 (en) | 2002-08-06 |
| WO2002059296A3 (en) | 2003-05-01 |
| EP1360308B1 (en) | 2007-10-10 |
| DE60222857D1 (de) | 2007-11-22 |
| AU2002227882C1 (en) | 2002-08-06 |
| NZ527208A (en) | 2005-05-27 |
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