DE60131837D1 - Analyse der fluoreszenzintensitäts- und lebensdauerverteilung - Google Patents

Analyse der fluoreszenzintensitäts- und lebensdauerverteilung

Info

Publication number
DE60131837D1
DE60131837D1 DE60131837T DE60131837T DE60131837D1 DE 60131837 D1 DE60131837 D1 DE 60131837D1 DE 60131837 T DE60131837 T DE 60131837T DE 60131837 T DE60131837 T DE 60131837T DE 60131837 D1 DE60131837 D1 DE 60131837D1
Authority
DE
Germany
Prior art keywords
particles
function
determining
argument
photon counts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60131837T
Other languages
English (en)
Other versions
DE60131837T2 (de
Inventor
Peet Kask
Karsten Gall
Kaupo Palo
Leif Brand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Application granted granted Critical
Publication of DE60131837D1 publication Critical patent/DE60131837D1/de
Publication of DE60131837T2 publication Critical patent/DE60131837T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N15/0211—Investigating a scatter or diffraction pattern
    • G01N2015/0216—Investigating a scatter or diffraction pattern from fluctuations of diffraction pattern
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
DE60131837T 2000-02-10 2001-02-10 Analyse der fluoreszenzintensitäts- und lebensdauerverteilung Expired - Lifetime DE60131837T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18154800P 2000-02-10 2000-02-10
US181548P 2000-02-10
PCT/EP2001/001470 WO2001059436A2 (en) 2000-02-10 2001-02-10 Fluorescence intensity and lifetime distribution analysis

Publications (2)

Publication Number Publication Date
DE60131837D1 true DE60131837D1 (de) 2008-01-24
DE60131837T2 DE60131837T2 (de) 2008-11-27

Family

ID=22664753

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60131837T Expired - Lifetime DE60131837T2 (de) 2000-02-10 2001-02-10 Analyse der fluoreszenzintensitäts- und lebensdauerverteilung

Country Status (9)

Country Link
US (1) US6690463B2 (de)
EP (1) EP1254362B1 (de)
JP (1) JP4776854B2 (de)
AT (1) ATE381013T1 (de)
AU (1) AU2001239258A1 (de)
DE (1) DE60131837T2 (de)
DK (1) DK1254362T3 (de)
ES (1) ES2298221T3 (de)
WO (1) WO2001059436A2 (de)

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DE10144435B4 (de) * 2001-09-06 2005-03-24 EuroPhoton GmbH Gesellschaft für optische Sensorik Verfahren zur Charakterisierung der Eigenschaften von fluoreszierenden Proben, insbesondere lebenden Zellen und Geweben, in multi-well, in in-vitro Fluoreszenz-Assays, in DNA-Chips, Vorrichtungen zur Durchführung des Verfahrens und deren Verwendung
CA2494478A1 (en) * 2002-08-01 2004-02-12 Sensor Technologies Llc Fluorescence correlation spectroscopy instrument
US20060237665A1 (en) * 2003-03-10 2006-10-26 Barney William S Bioaerosol discrimination
WO2004081548A1 (en) * 2003-03-10 2004-09-23 Tiax Llc System and method for bioaerosol discrimination by time-resolved fluorescence
EP1670943B1 (de) 2003-09-30 2013-11-13 Evotec International GmbH Diagnostische und therapeutische verwendung einer sulfotransferase für alzheimer-krankheit
JP3793531B2 (ja) * 2003-10-07 2006-07-05 オリンパス株式会社 蛍光寿命測定装置
CA2542774A1 (en) * 2003-10-18 2005-05-12 Bayer Healthcare Ag Direct observation of molecular modifications in biological test systems by measuring fluorescence lifetime
EP1528379A1 (de) * 2003-10-27 2005-05-04 Evotec Technologies GmbH Verfahren zum Auflösen von Spezies von fluoreszierenden Molekülen in einer Probe
JP4323369B2 (ja) * 2004-04-09 2009-09-02 オリンパス株式会社 走査型蛍光顕微鏡装置
US20050231717A1 (en) * 2004-04-16 2005-10-20 Industrial Technology Research Institute Fluorescence inspection spectrometer
US7332700B2 (en) * 2004-04-26 2008-02-19 Bruce Masato Ishimoto Photomultiplier tube with dynode modulation for photon-counting
US20080301821A1 (en) 2004-08-11 2008-12-04 Evotec Neurosciences Gmbh Diagnostic and Therapeutic use of a Plasma Membrane Atpase
JP2006133164A (ja) * 2004-11-09 2006-05-25 Fyuuensu:Kk カルモデュリンの構造変化を検出する方法、カルモデュリンの構造変化に影響を与える活性を有する物質を探索する方法
JP2008545402A (ja) 2005-05-27 2008-12-18 エボテツク・ニユーロサイエンシーズ・ゲー・エム・ベー・ハー 神経変性疾患の診断及び治療ターゲットとしてのｋｃｎｎ３
WO2006128879A1 (en) 2005-05-30 2006-12-07 Takeda Pharmaceutical Company Limited Diagnostic and therapeutic target prkx proteins for neurodegenerative diseases
WO2006128902A1 (en) 2005-06-01 2006-12-07 Evotec Neurosciences Gmbh Diagnostic and therapeutic target slc39a12 proteins for neurodegenerative diseases
JP2008546379A (ja) 2005-06-16 2008-12-25 エボテツク・ニユーロサイエンシーズ・ゲー・エム・ベー・ハー 神経変性疾患のための診断および治療の標的ａｄａｒｂ２タンパク質
GB2434483A (en) 2006-01-20 2007-07-25 Fianium Ltd High-Power Short Optical Pulse Source
FR2896877A1 (fr) * 2006-01-31 2007-08-03 Commissariat Energie Atomique Appareil et procede de caracterisation d'un systeme de comptage de particules elementaires
EP1930716A1 (de) 2006-12-08 2008-06-11 Micronas GmbH Verfahren und Vorrichtung zur Detektion eines Liganden in einem Fluid
JP5354938B2 (ja) * 2007-05-02 2013-11-27 オリンパス株式会社 蛍光顕微鏡装置
GB2451442B (en) * 2007-07-30 2013-03-06 Lein Applied Diagnostics Ltd Optical measurement apparatus and method therefor
GB0800936D0 (en) 2008-01-19 2008-02-27 Fianium Ltd A source of optical supercontinuum generation having a selectable pulse repetition frequency
DE102008045886A1 (de) * 2008-09-03 2010-03-04 Friedrich-Schiller-Universität Jena Verfahren zur exakten Bestimmung der Fluoreszenz in einem Schichtsystem, beispielsweise dem Auge
EP2340429B1 (de) * 2008-10-14 2022-08-24 Tissuevision, Inc. Vorrichtungen und verfahren für direktabtastende analoge zeitaufgelöse detektion
EP2186827A1 (de) 2008-11-14 2010-05-19 HS LifeSciences Ltd. Ersatzmarkergerichtetes cDNA-Klonen von selektiv induzierten mRNAs
US8611535B2 (en) * 2010-09-15 2013-12-17 At&T Intellectual Property I, L.P. Characterization of an entangled photon system
DE102011055330A1 (de) 2011-11-14 2013-05-16 Leica Microsystems Cms Gmbh Verfahren zum Messen der Lebensdauer eines angeregten Zustandes in einer Probe
US8981318B1 (en) * 2011-12-30 2015-03-17 Gene Capture, Inc. Multi-dimensional scanner for nano-second time scale signal detection
US8743356B1 (en) 2012-11-22 2014-06-03 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of National Defence Man-portable device for detecting hazardous material
DE102014017006B4 (de) * 2014-11-17 2019-07-11 Technische Universität Ilmenau Verfahren zur Bestimmung und Auswertung zeitaufgelöster Fluoreszenz- oder Reflexionsbilder an ausgedehnten dreidimensionalen Oberflächen
KR101859172B1 (ko) * 2016-12-22 2018-05-16 (주) 인텍플러스 아날로그 평균지연 방법에서 수집한 신호로부터 가상의 형광분포 모델을 통해 최소자승오차를 계산하여 2개 이상의 형광수명 성분을 구하는 형광수명 측정장치 및 그 측정방법
KR20230047328A (ko) 2020-08-06 2023-04-07 하마마츠 포토닉스 가부시키가이샤 시간 계측 장치, 형광 수명 계측 장치, 및 시간 계측 방법
WO2022030062A1 (ja) 2020-08-06 2022-02-10 浜松ホトニクス株式会社 時間計測装置、蛍光寿命計測装置、及び時間計測方法
DE102022121505A1 (de) 2022-08-25 2024-03-07 Carl Zeiss Meditec Ag Verfahren, Computerprogramm und Datenverarbeitungseinheit zur Vorbereitung der Beobachtung einer Fluoreszenzintensität, Verfahren zum Beobachten einer Fluoreszenzintensität und optisches Beobachtungssystem

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US5323008A (en) 1992-03-23 1994-06-21 Diatron Corporation Fluorometer detection system
EP0836090A1 (de) 1996-10-12 1998-04-15 Evotec BioSystems GmbH Probenanalyseverfahren durch Bestimmung der spezifischen Helligkeitsverteilung von Teilchen
JP3830167B2 (ja) * 1996-11-27 2006-10-04 マックス−プランク−ゲゼルシャフト ツル フェルデルング デァ ヴィッセンシャフテン エー．フォオ．，ベルリン 試料媒体にある標的粒子の所定の特性を決定するための方法および装置
EP0884583A1 (de) * 1997-06-10 1998-12-16 Evotec BioSystems GmbH Verfahren zur Charakterisierung von Proben in einem mindestens zweidimensionalen Raum spezifischer physikalischer Eigenschaften
US6556296B1 (en) * 1997-09-29 2003-04-29 Evotec Biosystems Ag Method for characterizing samples by determination of a function of at least one specific property of particles in a sample
EP0945718A1 (de) 1998-03-21 1999-09-29 Evotec BioSystems AG Ein Verfahren zur Probencharakterisierung durch Bestimmung einer Funktion mindestens einer spezifischen Eigenschaft von Einheiten der Probe
KR100618502B1 (ko) * 1998-03-16 2006-09-01 지이 헬스케어 바이오-사이언시즈 코프. 공초점 마이크로스코피 영상 시스템에서 사용하기 위한 전자기 방출의 집속 시스템 및 방법
DE60001731T2 (de) * 1999-04-29 2004-02-05 Evotec Oai Ag Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen
US6376843B1 (en) * 1999-06-23 2002-04-23 Evotec Oai Ag Method of characterizing fluorescent molecules or other particles using generating functions

Also Published As

Publication number Publication date
EP1254362A2 (de) 2002-11-06
ATE381013T1 (de) 2007-12-15
JP2003522946A (ja) 2003-07-29
AU2001239258A1 (en) 2001-08-20
US20020063863A1 (en) 2002-05-30
US6690463B2 (en) 2004-02-10
EP1254362B1 (de) 2007-12-12
DK1254362T3 (da) 2008-05-05
DE60131837T2 (de) 2008-11-27
WO2001059436A3 (en) 2002-03-14
JP4776854B2 (ja) 2011-09-21
ES2298221T3 (es) 2008-05-16
WO2001059436A2 (en) 2001-08-16

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