DE60116500T2 - Dna/rna-einbettungsmedium und verfahren zur verwendung - Google Patents
Dna/rna-einbettungsmedium und verfahren zur verwendung Download PDFInfo
- Publication number
- DE60116500T2 DE60116500T2 DE60116500T DE60116500T DE60116500T2 DE 60116500 T2 DE60116500 T2 DE 60116500T2 DE 60116500 T DE60116500 T DE 60116500T DE 60116500 T DE60116500 T DE 60116500T DE 60116500 T2 DE60116500 T2 DE 60116500T2
- Authority
- DE
- Germany
- Prior art keywords
- dna
- rna
- pcr
- medium
- hpmc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000007654 immersion Methods 0.000 title 1
- 230000003321 amplification Effects 0.000 claims abstract description 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 15
- 230000003204 osmotic effect Effects 0.000 claims abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- 238000003752 polymerase chain reaction Methods 0.000 claims description 43
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 36
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 36
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 36
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 36
- 238000004321 preservation Methods 0.000 claims description 19
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 12
- 238000010240 RT-PCR analysis Methods 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 230000000087 stabilizing effect Effects 0.000 claims description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- 230000004544 DNA amplification Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
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- 239000003761 preservation solution Substances 0.000 claims 2
- 229920002678 cellulose Polymers 0.000 abstract description 10
- 239000001913 cellulose Substances 0.000 abstract description 10
- 239000003381 stabilizer Substances 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 54
- 210000004027 cell Anatomy 0.000 description 46
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 39
- 238000003757 reverse transcription PCR Methods 0.000 description 21
- 210000001519 tissue Anatomy 0.000 description 19
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- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 7
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 7
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- 108700005091 Immunoglobulin Genes Proteins 0.000 description 4
- 238000002944 PCR assay Methods 0.000 description 4
- 108091092330 cytoplasmic RNA Proteins 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
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- 229920000936 Agarose Polymers 0.000 description 2
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 239000001856 Ethyl cellulose Substances 0.000 description 2
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 2
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 2
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 2
- 229920001479 Hydroxyethyl methyl cellulose Polymers 0.000 description 2
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 208000030289 Lymphoproliferative disease Diseases 0.000 description 2
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- 229920001249 ethyl cellulose Polymers 0.000 description 2
- 235000019325 ethyl cellulose Nutrition 0.000 description 2
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 2
- 229920013819 hydroxyethyl ethylcellulose Polymers 0.000 description 2
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 2
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 229920000609 methyl cellulose Polymers 0.000 description 2
- 239000001923 methylcellulose Substances 0.000 description 2
- 235000010981 methylcellulose Nutrition 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 208000003950 B-cell lymphoma Diseases 0.000 description 1
- 102000006734 Beta-Globulins Human genes 0.000 description 1
- 108010087504 Beta-Globulins Proteins 0.000 description 1
- 241000357209 Cordia subcordata Species 0.000 description 1
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000017604 Hodgkin disease Diseases 0.000 description 1
- 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 206010042971 T-cell lymphoma Diseases 0.000 description 1
- 208000027585 T-cell non-Hodgkin lymphoma Diseases 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 229940071125 manganese acetate Drugs 0.000 description 1
- UOGMEBQRZBEZQT-UHFFFAOYSA-L manganese(2+);diacetate Chemical compound [Mn+2].CC([O-])=O.CC([O-])=O UOGMEBQRZBEZQT-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- XEBWQGVWTUSTLN-UHFFFAOYSA-M phenylmercury acetate Chemical compound CC(=O)O[Hg]C1=CC=CC=C1 XEBWQGVWTUSTLN-UHFFFAOYSA-M 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 239000002342 ribonucleoside Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- YEENEYXBHNNNGV-XEHWZWQGSA-M sodium;3-acetamido-5-[acetyl(methyl)amino]-2,4,6-triiodobenzoate;(2r,3r,4s,5s,6r)-2-[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound [Na+].CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I.O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 YEENEYXBHNNNGV-XEHWZWQGSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- -1 vanadyl ribonucleoside Chemical compound 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US605611 | 1990-10-30 | ||
| US60561100A | 2000-06-28 | 2000-06-28 | |
| PCT/SE2001/001468 WO2002000848A1 (en) | 2000-06-28 | 2001-06-27 | Dna/rna-embedding medium and method of use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60116500D1 DE60116500D1 (de) | 2006-03-30 |
| DE60116500T2 true DE60116500T2 (de) | 2006-09-21 |
Family
ID=24424431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60116500T Expired - Lifetime DE60116500T2 (de) | 2000-06-28 | 2001-06-27 | Dna/rna-einbettungsmedium und verfahren zur verwendung |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6706499B2 (https=) |
| EP (1) | EP1303586B1 (https=) |
| JP (1) | JP2004506414A (https=) |
| AT (1) | ATE315080T1 (https=) |
| AU (2) | AU8033401A (https=) |
| CA (1) | CA2413916C (https=) |
| DE (1) | DE60116500T2 (https=) |
| WO (1) | WO2002000848A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1505871B1 (en) * | 2002-05-10 | 2013-04-24 | University of Miami | Preservation of rna and morphology in cells and tissues |
| DE60334471D1 (de) | 2002-10-11 | 2010-11-18 | Univ Erasmus | Primer für nukleinsäureamplifikation in pcr-basierten klonalitätsstudien |
| JP2008538752A (ja) * | 2005-04-12 | 2008-11-06 | クレオ コスメティック アンド ファーマスーティカルカンパニー エルエルシー | 角膜組織を生体外保存するための組成物および方法 |
| EP2162531B1 (en) | 2007-05-04 | 2019-01-16 | Ascendia AB | Method and means for culturing osteoblastic cells |
| US9587268B2 (en) * | 2014-01-29 | 2017-03-07 | Agilent Technologies Inc. | Fast hybridization for next generation sequencing target enrichment |
| WO2022005900A1 (en) | 2020-07-01 | 2022-01-06 | Illumina, Inc. | Nucleic acid capture, concentration, and purification |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63216476A (ja) * | 1987-03-05 | 1988-09-08 | Rikagaku Kenkyusho | 無血清凍結保存用培地 |
| US5399493A (en) * | 1989-06-15 | 1995-03-21 | The Regents Of The University Of Michigan | Methods and compositions for the optimization of human hematopoietic progenitor cell cultures |
| US5612218A (en) | 1991-12-03 | 1997-03-18 | Busch; Christer | Method of handling biologic matter and means for carrying out the method |
| JPH07255469A (ja) * | 1994-03-17 | 1995-10-09 | Kurabo Ind Ltd | 動物細胞の凍結保存液 |
| SE9700983D0 (sv) * | 1997-03-18 | 1997-03-18 | Ascendia Ab | Stimulering, odling och preservation av pankreas- och andra celler |
-
2001
- 2001-06-27 WO PCT/SE2001/001468 patent/WO2002000848A1/en not_active Ceased
- 2001-06-27 EP EP01958713A patent/EP1303586B1/en not_active Expired - Lifetime
- 2001-06-27 AT AT01958713T patent/ATE315080T1/de not_active IP Right Cessation
- 2001-06-27 DE DE60116500T patent/DE60116500T2/de not_active Expired - Lifetime
- 2001-06-27 JP JP2002505964A patent/JP2004506414A/ja active Pending
- 2001-06-27 AU AU8033401A patent/AU8033401A/xx active Pending
- 2001-06-27 AU AU2001280334A patent/AU2001280334B2/en not_active Ceased
- 2001-06-27 CA CA002413916A patent/CA2413916C/en not_active Expired - Fee Related
- 2001-11-13 US US10/011,119 patent/US6706499B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002000848A1 (en) | 2002-01-03 |
| US20020150928A1 (en) | 2002-10-17 |
| JP2004506414A (ja) | 2004-03-04 |
| CA2413916C (en) | 2007-09-18 |
| DE60116500D1 (de) | 2006-03-30 |
| EP1303586B1 (en) | 2006-01-04 |
| US6706499B2 (en) | 2004-03-16 |
| EP1303586A1 (en) | 2003-04-23 |
| ATE315080T1 (de) | 2006-02-15 |
| CA2413916A1 (en) | 2002-01-03 |
| AU2001280334B2 (en) | 2005-05-05 |
| AU8033401A (en) | 2002-01-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |