AU2001280334B2 - DNA/rna-embedding medium and method of use - Google Patents
DNA/rna-embedding medium and method of use Download PDFInfo
- Publication number
- AU2001280334B2 AU2001280334B2 AU2001280334A AU2001280334A AU2001280334B2 AU 2001280334 B2 AU2001280334 B2 AU 2001280334B2 AU 2001280334 A AU2001280334 A AU 2001280334A AU 2001280334 A AU2001280334 A AU 2001280334A AU 2001280334 B2 AU2001280334 B2 AU 2001280334B2
- Authority
- AU
- Australia
- Prior art keywords
- dna
- rna
- pcr
- native
- cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims abstract description 24
- 229920002678 cellulose Polymers 0.000 claims abstract description 18
- 239000001913 cellulose Substances 0.000 claims abstract description 18
- 230000003321 amplification Effects 0.000 claims abstract description 16
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 16
- 230000003204 osmotic effect Effects 0.000 claims abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 8
- 238000003752 polymerase chain reaction Methods 0.000 claims description 36
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 27
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 27
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 27
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 27
- 235000010980 cellulose Nutrition 0.000 claims description 17
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- 238000010240 RT-PCR analysis Methods 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- LNAZSHAWQACDHT-XIYTZBAFSA-N (2r,3r,4s,5r,6s)-4,5-dimethoxy-2-(methoxymethyl)-3-[(2s,3r,4s,5r,6r)-3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6r)-4,5,6-trimethoxy-2-(methoxymethyl)oxan-3-yl]oxyoxane Chemical compound CO[C@@H]1[C@@H](OC)[C@H](OC)[C@@H](COC)O[C@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@H](O[C@H]2[C@@H]([C@@H](OC)[C@H](OC)O[C@@H]2COC)OC)O[C@@H]1COC LNAZSHAWQACDHT-XIYTZBAFSA-N 0.000 claims description 3
- 239000001856 Ethyl cellulose Substances 0.000 claims description 3
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 claims description 3
- 229920001479 Hydroxyethyl methyl cellulose Polymers 0.000 claims description 3
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 3
- 229920001249 ethyl cellulose Polymers 0.000 claims description 3
- 235000019325 ethyl cellulose Nutrition 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 229920013819 hydroxyethyl ethylcellulose Polymers 0.000 claims description 3
- 239000001863 hydroxypropyl cellulose Substances 0.000 claims description 3
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 claims description 3
- 229920000609 methyl cellulose Polymers 0.000 claims description 3
- 239000001923 methylcellulose Substances 0.000 claims description 3
- 235000010981 methylcellulose Nutrition 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000001103 potassium chloride Substances 0.000 claims description 2
- 235000011164 potassium chloride Nutrition 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 abstract description 6
- 108020004414 DNA Proteins 0.000 description 54
- 210000004027 cell Anatomy 0.000 description 50
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 39
- 238000004321 preservation Methods 0.000 description 24
- 210000001519 tissue Anatomy 0.000 description 19
- 239000012634 fragment Substances 0.000 description 12
- 108091093088 Amplicon Proteins 0.000 description 11
- 238000003860 storage Methods 0.000 description 10
- 210000003719 b-lymphocyte Anatomy 0.000 description 9
- 239000003550 marker Substances 0.000 description 9
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 8
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 8
- 102000007469 Actins Human genes 0.000 description 7
- 108010085238 Actins Proteins 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 239000008188 pellet Substances 0.000 description 7
- 238000003556 assay Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 108700005091 Immunoglobulin Genes Proteins 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 4
- 238000002944 PCR assay Methods 0.000 description 4
- 230000036210 malignancy Effects 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 108010067770 Endopeptidase K Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 230000001351 cycling effect Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 2
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 101100188555 Arabidopsis thaliana OCT6 gene Proteins 0.000 description 1
- 208000003950 B-cell lymphoma Diseases 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
- 102000020897 Formins Human genes 0.000 description 1
- 108091022623 Formins Proteins 0.000 description 1
- 208000017604 Hodgkin disease Diseases 0.000 description 1
- 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 208000030289 Lymphoproliferative disease Diseases 0.000 description 1
- 238000013494 PH determination Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000011509 clonal analysis Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 229940071125 manganese acetate Drugs 0.000 description 1
- UOGMEBQRZBEZQT-UHFFFAOYSA-L manganese(2+);diacetate Chemical compound [Mn+2].CC([O-])=O.CC([O-])=O UOGMEBQRZBEZQT-UHFFFAOYSA-L 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- XEBWQGVWTUSTLN-UHFFFAOYSA-M phenylmercury acetate Chemical compound CC(=O)O[Hg]C1=CC=CC=C1 XEBWQGVWTUSTLN-UHFFFAOYSA-M 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000013777 protein digestion Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- YEENEYXBHNNNGV-XEHWZWQGSA-M sodium;3-acetamido-5-[acetyl(methyl)amino]-2,4,6-triiodobenzoate;(2r,3r,4s,5s,6r)-2-[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound [Na+].CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I.O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 YEENEYXBHNNNGV-XEHWZWQGSA-M 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 125000005287 vanadyl group Chemical group 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US60561100A | 2000-06-28 | 2000-06-28 | |
| US09/605,611 | 2000-06-28 | ||
| PCT/SE2001/001468 WO2002000848A1 (en) | 2000-06-28 | 2001-06-27 | Dna/rna-embedding medium and method of use |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| AU2001280334A1 AU2001280334A1 (en) | 2002-03-28 |
| AU2001280334A2 AU2001280334A2 (en) | 2003-03-13 |
| AU2001280334B2 true AU2001280334B2 (en) | 2005-05-05 |
Family
ID=24424431
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001280334A Ceased AU2001280334B2 (en) | 2000-06-28 | 2001-06-27 | DNA/rna-embedding medium and method of use |
| AU8033401A Pending AU8033401A (en) | 2000-06-28 | 2001-06-27 | Dna/rna-embedding medium and method of use |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU8033401A Pending AU8033401A (en) | 2000-06-28 | 2001-06-27 | Dna/rna-embedding medium and method of use |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6706499B2 (https=) |
| EP (1) | EP1303586B1 (https=) |
| JP (1) | JP2004506414A (https=) |
| AT (1) | ATE315080T1 (https=) |
| AU (2) | AU2001280334B2 (https=) |
| CA (1) | CA2413916C (https=) |
| DE (1) | DE60116500T2 (https=) |
| WO (1) | WO2002000848A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1505871T3 (da) * | 2002-05-10 | 2013-05-13 | Univ Miami | Konservering af rna og morfologi i celler og væv |
| EP2418287B1 (en) * | 2002-10-11 | 2013-09-18 | Erasmus Universiteit Rotterdam | Nucleic acid amplification primers for PCR-based clonality studies of the TCR-beta gene |
| EP1868430B1 (en) * | 2005-04-12 | 2015-07-08 | Cleo Cosmetic and Pharmaceutical Co., LLC | Composition and method for in vitro preservation of corneal tissues |
| US10010649B2 (en) | 2007-05-04 | 2018-07-03 | Ascendia Ab | Method and means for culturing osteoblastic cells |
| US9587268B2 (en) * | 2014-01-29 | 2017-03-07 | Agilent Technologies Inc. | Fast hybridization for next generation sequencing target enrichment |
| MX2022014719A (es) | 2020-07-01 | 2023-03-06 | Illumina Inc | Captura, concentración y purificación de ácido nucleico. |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63216476A (ja) * | 1987-03-05 | 1988-09-08 | Rikagaku Kenkyusho | 無血清凍結保存用培地 |
| US5399493A (en) * | 1989-06-15 | 1995-03-21 | The Regents Of The University Of Michigan | Methods and compositions for the optimization of human hematopoietic progenitor cell cultures |
| US5612218A (en) | 1991-12-03 | 1997-03-18 | Busch; Christer | Method of handling biologic matter and means for carrying out the method |
| JPH07255469A (ja) * | 1994-03-17 | 1995-10-09 | Kurabo Ind Ltd | 動物細胞の凍結保存液 |
| SE9700983D0 (sv) * | 1997-03-18 | 1997-03-18 | Ascendia Ab | Stimulering, odling och preservation av pankreas- och andra celler |
-
2001
- 2001-06-27 WO PCT/SE2001/001468 patent/WO2002000848A1/en not_active Ceased
- 2001-06-27 AU AU2001280334A patent/AU2001280334B2/en not_active Ceased
- 2001-06-27 EP EP01958713A patent/EP1303586B1/en not_active Expired - Lifetime
- 2001-06-27 AT AT01958713T patent/ATE315080T1/de not_active IP Right Cessation
- 2001-06-27 JP JP2002505964A patent/JP2004506414A/ja active Pending
- 2001-06-27 CA CA002413916A patent/CA2413916C/en not_active Expired - Fee Related
- 2001-06-27 AU AU8033401A patent/AU8033401A/xx active Pending
- 2001-06-27 DE DE60116500T patent/DE60116500T2/de not_active Expired - Lifetime
- 2001-11-13 US US10/011,119 patent/US6706499B2/en not_active Expired - Lifetime
Non-Patent Citations (1)
| Title |
|---|
| Nil Relevant * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20020150928A1 (en) | 2002-10-17 |
| EP1303586B1 (en) | 2006-01-04 |
| EP1303586A1 (en) | 2003-04-23 |
| DE60116500T2 (de) | 2006-09-21 |
| DE60116500D1 (de) | 2006-03-30 |
| AU8033401A (en) | 2002-01-08 |
| US6706499B2 (en) | 2004-03-16 |
| CA2413916C (en) | 2007-09-18 |
| WO2002000848A1 (en) | 2002-01-03 |
| JP2004506414A (ja) | 2004-03-04 |
| ATE315080T1 (de) | 2006-02-15 |
| CA2413916A1 (en) | 2002-01-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11008629B1 (en) | Rapid diagnostic test using colorimetric LAMP | |
| US7803929B2 (en) | Kits for diagnosis and monitoring of pathogenic infection by analysis of cell-free pathogenic nucleic acids in urine | |
| KR100723328B1 (ko) | 고정되고 파라핀 포매된 조직 표본으로부터 ｒｎａ를 분리하는 방법 | |
| WO2004072230A2 (en) | Real-time polymerase chain reaction using large target amplicons | |
| US8895240B2 (en) | Method, kit and system for collecting and processing samples for DNA and RNA detection | |
| JPWO2005012518A1 (ja) | 核酸検出用キット | |
| JP5926194B2 (ja) | Ｆｅｃｖ及びｆｉｐｖを区別する為の手段及び方法 | |
| GB2457418A (en) | Disposable articles for analysis and diagnostics for a laboratory | |
| BRPI0715982A2 (pt) | par de sequÊncias do primier diagnàstico de vibro harveyi, kit para a detecÇço do vibrio harveyi mÉtodospara a detecÇço da presenÇa do vibrio harveyi e mÉtodo para q quantificaÇço da quantidade de vibrio harveyi | |
| Sirakov | Nucleic acid isolation and downstream applications | |
| AU2001280334B2 (en) | DNA/rna-embedding medium and method of use | |
| JP5099920B2 (ja) | 核酸試験用対照 | |
| US20240309434A1 (en) | Targeted rare allele crispr enrichment | |
| Gruber et al. | Detection of bovine viral diarrhea virus RNA in formalin-fixed, paraffin-embedded brain tissue by nested polymerase chain reaction | |
| AU2001280334A1 (en) | DNA/RNA-embedding medium and method of use | |
| AU2001280334A2 (en) | DNA/RNA-embedding medium and method of use | |
| Gray et al. | Detection of channel catfish virus DNA in acutely infected channel catfish, Ictalurus punctatus (Rafinesque), using the polymerase chain reaction | |
| JP3494509B2 (ja) | 核酸合成法 | |
| KR101655069B1 (ko) | 잉어 허피스 바이러스 그레이 에스피에이치 판별용 유전자 마커, 및 이를 이용한 원인바이러스의 판별 방법 | |
| KR101911017B1 (ko) | 타일로렐라 에퀴제니탈리스의 검출용 조성물, 타일로렐라 에퀴제니탈리스 감염에 의한 말전염성자궁염의 진단용 조성물, 타일로렐라 에퀴제니탈리스의 검출방법, 및 타일로렐라 에퀴제니탈리스 감염에 의한 말전염성자궁염의 진단방법 | |
| JP2982304B2 (ja) | 核酸の識別方法及び核酸の識別用検査セット | |
| KR101644776B1 (ko) | 참돔 이리도바이러스 검출용 유전자 마커, 및 이를 이용한 원인바이러스의 검출 방법 | |
| JP2025097106A (ja) | リゾチームによる核酸増幅阻害の抑制に用いるための組成物およびその用途 | |
| SU1659488A1 (ru) | Способ определени генетический вариантов каппа-казеина у сельскохоз йственных животных | |
| KR101967733B1 (ko) | 테트라사이클린계 항생제 내성균 판별용 pna 프로브 및 이를 이용한 항생제 내성균 판별 방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 16 DEC 2002 |
|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |