DE60044876D1 - Zur mutation geeignete einzelsträngige oligonukleotid vektoren - Google Patents

Zur mutation geeignete einzelsträngige oligonukleotid vektoren

Info

Publication number
DE60044876D1
DE60044876D1 DE60044876T DE60044876T DE60044876D1 DE 60044876 D1 DE60044876 D1 DE 60044876D1 DE 60044876 T DE60044876 T DE 60044876T DE 60044876 T DE60044876 T DE 60044876T DE 60044876 D1 DE60044876 D1 DE 60044876D1
Authority
DE
Germany
Prior art keywords
effective
oligodeoxynucleotides
mutational
sided
oligonucleotide vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60044876T
Other languages
English (en)
Inventor
Richard A Metz
Bruce L Frank
Debra M Walther
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valigen US Inc
Original Assignee
Valigen US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valigen US Inc filed Critical Valigen US Inc
Application granted granted Critical
Publication of DE60044876D1 publication Critical patent/DE60044876D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE60044876T 1999-08-27 2000-08-25 Zur mutation geeignete einzelsträngige oligonukleotid vektoren Expired - Lifetime DE60044876D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/384,960 US6271360B1 (en) 1999-08-27 1999-08-27 Single-stranded oligodeoxynucleotide mutational vectors
PCT/US2000/023457 WO2001015740A1 (en) 1999-08-27 2000-08-25 Single-stranded oligodeoxynucleotide mutational vectors

Publications (1)

Publication Number Publication Date
DE60044876D1 true DE60044876D1 (de) 2010-10-07

Family

ID=23519459

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60044876T Expired - Lifetime DE60044876D1 (de) 1999-08-27 2000-08-25 Zur mutation geeignete einzelsträngige oligonukleotid vektoren

Country Status (15)

Country Link
US (3) US6271360B1 (de)
EP (3) EP2266627B1 (de)
JP (2) JP5697187B2 (de)
CN (1) CN1384759B (de)
AT (1) ATE478687T1 (de)
AU (2) AU773242C (de)
BR (1) BR0013590A (de)
CA (1) CA2382120C (de)
DE (1) DE60044876D1 (de)
DK (3) DK2324856T3 (de)
ES (3) ES2358292T3 (de)
NZ (1) NZ517942A (de)
PT (3) PT2324856E (de)
WO (1) WO2001015740A1 (de)
ZA (1) ZA200201228B (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6652607B2 (en) 1999-07-07 2003-11-25 The Lubrizol Corporation Concentrated emulsion for making an aqueous hydrocarbon fuel
EP1212406A1 (de) * 1999-08-20 2002-06-12 University Of Delaware Zellfreies testverfahren für zielgerichtete genveränderungen und konversionen von pflanzengenen
US6271360B1 (en) * 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
AR025996A1 (es) 1999-10-07 2002-12-26 Valigen Us Inc Plantas no transgenicas resistentes a los herbicidas.
US6936467B2 (en) 2000-03-27 2005-08-30 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
KR20030003240A (ko) * 2000-03-27 2003-01-09 유니버시티 오브 델라웨어 개질된 단일 가닥 올리고뉴클레오티드를 이용한 표적염색체 게놈 변경법
CA2409172A1 (en) * 2000-05-17 2001-11-22 University Of Delaware Plant gene targeting using oligonucleotides
IL154099A0 (en) * 2000-07-27 2003-07-31 Univ Delaware A method and kit for enhancing oligonucleotide-mediated gene alteration
US7053195B1 (en) * 2001-06-12 2006-05-30 Syngenta Participatious Ag Locked nucleic acid containing heteropolymers and related methods
US20040096880A1 (en) * 2001-08-07 2004-05-20 Kmiec Eric B. Compositions and methods for the treatment of diseases exhibiting protein misassembly and aggregation
WO2003013437A2 (en) * 2001-08-07 2003-02-20 University Of Delaware Compositions and methods for the prevention and treatment of huntington's disease
EP1446503A2 (de) * 2001-09-27 2004-08-18 University Of Delaware Zusammensetzung und verfahren zur verbesserung der oligonukleotidgesteuerten veränderung von nukleinsäuresequenzen
WO2003027264A2 (en) * 2001-09-27 2003-04-03 University Of Delaware Coisogenic eukaryotic cell collections
WO2003075856A2 (en) * 2002-03-07 2003-09-18 University Of Delaware Methods, compositions, and kits for enhancing oligonucleotide-mediated nucleic acid sequence alteration using compositions comprising a histone deacetylase inhibitor, lambda phage beta protein, or hydroxyurea
GB0218803D0 (en) * 2002-08-13 2002-09-18 Nl Kanker I Targeted gene modification by single-standed DNA oligonucleotides
WO2004033708A2 (en) * 2002-10-07 2004-04-22 University Of Delaware Methods and compositions for reducing screening in oligonucleotide-directed nucleic acid sequence alteration
US6929917B2 (en) * 2002-11-18 2005-08-16 Pregentis Method for cloning of a rare, specifically mutated cell
US20070072815A1 (en) * 2004-05-04 2007-03-29 Kmiec Eric B Methods and kits to increase the efficiency of oligonucleotide-directed nucleic acid sequence alteration
PT2465341E (pt) 2006-01-12 2015-03-09 Cibus Europe Bv Mutantes de epsps
RU2483057C2 (ru) 2006-06-28 2013-05-27 Ньюселис Инк. Смеси жирных кислот и их применение
US8710299B2 (en) 2006-10-06 2014-04-29 Basf Plant Science Gmbh Processes for producing polyunsaturated fatty acids in transgenic organisms
US9102948B2 (en) 2006-11-17 2015-08-11 22Nd Century Limited, Llc Regulating alkaloids
PL2700721T3 (pl) * 2007-10-05 2019-06-28 Cibus Europe B.V. Zmutowane geny kodujące syntazę kwasu acetohydroksylowego w brassica
CA2710767C (en) * 2007-12-27 2016-09-27 Cibus Llc Alkylester fatty acid blends and uses therefor
EA201071338A1 (ru) * 2008-05-23 2012-01-30 Сибас Ойлз, Ллс Получение сквалена с применением дрожжей
PL2342331T3 (pl) 2008-09-26 2016-05-31 Basf Agrochemical Products Bv Mutanty ahas odporne na herbicydy i sposoby ich zastosowania
WO2010066703A2 (en) 2008-12-12 2010-06-17 Basf Plant Science Gmbh Desaturases and process for the production of polyunsaturated fatty acids in transgenic organisms
EP2504421B1 (de) 2009-11-23 2017-02-15 Nucelis Inc. Verfahren und zusammensetzungen zur herstellung von squalen mit hefe
US20120122223A1 (en) 2010-08-03 2012-05-17 Cibus Us Llc Mutated protoporphyrinogen ix oxidase (ppx) genes
UA112969C2 (uk) 2010-08-03 2016-11-25 Сібас Юс Ллс Рослина, стійка до одного або більше ррх-інгібуючих гербіцидів, яка містить мутантний ген протопорфіриноген ix оксидази (ррх)
EP2426204A1 (de) 2010-09-02 2012-03-07 Ludwig-Maximilians-Universität München Spontane Knöllchenorganogenese in Pflanzen
WO2012028673A1 (en) 2010-09-02 2012-03-08 Ludwig-Maximilians-Universitaet Muenchen Spontaneous organogenesis in plants
KR101137285B1 (ko) 2010-10-28 2012-04-20 위월드 주식회사 초소형 광대역 송수신 안테나 피더
AU2013302947B2 (en) 2012-08-17 2017-06-01 Diaa ALABED Use of a maize untranslated region for transgene expression in plants
PE20151922A1 (es) 2013-03-14 2016-01-16 Cibus Us Llc Genes de aleno oxido sintasa 2 (aos2) mutados
HUE059269T2 (hu) 2013-03-15 2022-11-28 Cibus Us Llc Eljárások és készítmények a célzott génmódosítás hatékonyságának növelésére oligonukleotid-közvetített génjavítás alkalmazásával
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
LT2966984T (lt) 2013-03-15 2022-05-25 Cibus Us Llc Tikslinė genų modifikacija, naudojant oligonukleotidų sukeltą genų taisymą
KR102569558B1 (ko) 2014-03-14 2023-08-22 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
EA201891629A1 (ru) 2016-02-09 2019-03-29 Сибас Юс Ллс Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов
US10584363B2 (en) 2016-06-03 2020-03-10 Takara Bio Usa, Inc. Methods of producing and using single-stranded deoxyribonucleic acids and compositions for use in practicing the same
CN111819285B (zh) 2018-01-09 2024-08-09 希博斯美国有限公司 防碎基因和突变

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2731844B2 (ja) * 1987-04-22 1998-03-25 ザ・ユニヴァーシティー・オブ・コネチカット 哺乳動物の細胞にポリヌクレオチドを導入するキャリア及び水溶性高分子複合体並びにその導入方法
ATE151076T1 (de) * 1990-07-02 1997-04-15 Hoechst Ag Oligonucleotid-analoge mit terminalen 3'-3'-bzw. 5'-5'-internucleotidverknüpfungen
KR100252547B1 (ko) 1991-09-05 2000-09-01 프레드 마리얀스키 폴리-또는 올리고누클레오티드의 세포로의 표적화된 전달
US5556959A (en) 1993-01-22 1996-09-17 Pharmacia P-L Biochemicals Inc. Indocarbocyanine-linked phosphoramidites
US5808044A (en) * 1993-01-22 1998-09-15 Pharmacia Biotech Inc. Indocarbocyanine and benzindocarbocyanine phosphoramidites
AU691550B2 (en) 1993-12-09 1998-05-21 Thomas Jefferson University Compounds and methods for site-directed mutations in eukaryotic cells
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
AU6328696A (en) * 1995-06-07 1996-12-30 Yale University Treatment of hemoglobinopathies
US5908777A (en) * 1995-06-23 1999-06-01 University Of Pittsburgh Lipidic vector for nucleic acid delivery
FR2736926B1 (fr) 1995-07-19 1997-08-22 Rhone Poulenc Agrochimie 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene
US5731181A (en) 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
US5760012A (en) 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations
US5888983A (en) 1996-05-01 1999-03-30 Thomas Jefferson University Method and oligonucleobase compounds for curing diseases caused by mutations
NZ337445A (en) * 1997-02-26 2000-12-22 Gen Hospital Corp Transgenic animals and cell lines for screening drugs effective for the treatment or prevention of alzheimer's disease
US6524613B1 (en) 1997-04-30 2003-02-25 Regents Of The University Of Minnesota Hepatocellular chimeraplasty
EP0979311A1 (de) 1997-04-30 2000-02-16 Of The University Of Minnesota Regents $i(IN VIVO)VERWENDUNG REKOMBINAGENISCHER OLIGONUKLEOBASEN ZUR KORREKTUR GENETISCHER DEFEKTE IN HEPATOZYTEN
GB9711015D0 (en) 1997-05-28 1997-07-23 Zeneca Ltd Improvements in or relating to organic compounds
US5986086A (en) * 1997-06-20 1999-11-16 Amersham Pharmacia Biotech Inc. Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides
AU748015B2 (en) 1997-08-05 2002-05-30 Valigen (Us), Inc. The use of mixed duplex oligonucleotides to effect localized genetic changes in plants
AU1526199A (en) 1997-11-18 1999-06-07 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
US6004804A (en) 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
US6271360B1 (en) * 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
CN107124878B (zh) 2014-08-21 2020-09-29 康宁股份有限公司 防止层叠玻璃制品中的气泡的方法以及由此形成的层叠玻璃制品

Also Published As

Publication number Publication date
ES2544727T3 (es) 2015-09-03
CA2382120C (en) 2013-10-29
ZA200201228B (en) 2003-05-28
DK2324856T3 (en) 2015-08-03
JP2003508451A (ja) 2003-03-04
NZ517942A (en) 2004-07-30
EP2266627B1 (de) 2015-04-22
JP5813571B2 (ja) 2015-11-17
DK2266627T3 (da) 2015-06-15
PT1210123E (pt) 2010-11-30
ATE478687T1 (de) 2010-09-15
AU7076700A (en) 2001-03-26
WO2001015740A1 (en) 2001-03-08
PT2324856E (pt) 2015-09-03
AU2004205139A1 (en) 2004-09-16
US20040023392A1 (en) 2004-02-05
AU773242C (en) 2005-04-07
EP1210123A1 (de) 2002-06-05
ES2542530T3 (es) 2015-08-06
EP1210123A4 (de) 2004-08-18
PT2266627E (pt) 2015-08-25
US6271360B1 (en) 2001-08-07
ES2358292T3 (es) 2011-05-09
US6479292B1 (en) 2002-11-12
CN1384759B (zh) 2013-05-22
AU773242B2 (en) 2004-05-20
DK1210123T3 (da) 2010-12-20
JP2012184246A (ja) 2012-09-27
CA2382120A1 (en) 2001-03-08
JP5697187B2 (ja) 2015-04-08
EP2324856B1 (de) 2015-05-06
EP1210123B1 (de) 2010-08-25
BR0013590A (pt) 2002-05-07
EP2324856A1 (de) 2011-05-25
CN1384759A (zh) 2002-12-11
EP2266627A1 (de) 2010-12-29
US7060500B2 (en) 2006-06-13
AU2004205139B2 (en) 2008-01-10

Similar Documents

Publication Publication Date Title
DE60044876D1 (de) Zur mutation geeignete einzelsträngige oligonukleotid vektoren
US5565350A (en) Compounds and methods for site directed mutations in eukaryotic cells
CN108291218A (zh) 核酸酶非依赖性靶向基因编辑平台及其用途
Kivisaar Stationary phase mutagenesis: mechanisms that accelerate adaptation of microbial populations under environmental stress
CN107002093A (zh) 用于靶向遗传修饰的方法和组合物，以及这些组合物的使用方法
WO2019103442A3 (ko) CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도
HRP20240657T1 (hr) Materijali i postupci za liječenje hemoglobinopatija
CN107922949A (zh) 用于通过同源重组的基于crispr/cas的基因组编辑的化合物和方法
EP2267139A3 (de) Verfahren und Mittel zum Erhalt von modifizierten Phänotypen
ATE412054T1 (de) Delta-endotoxin gene und verfahren zu ihrer verwendung
BR0209745A (pt) Cromossomas artificiais de plantas, usos e dos mesmos e método para preparação de cromossomas artificiais de plantas
CN101346474A (zh) 用于改进的靶向核苷酸交换的可选择核苷酸
EP0827410A4 (de) Verfahren zür einführung und expression von genen in tierischen zellen
JP6958917B2 (ja) 遺伝子ノックイン細胞の作製方法
MX2010003891A (es) Genes sinteticos que codifican cry1ac.
US20240218358A1 (en) Prime editing-based gene editing composition with enhanced editing efficiency and use thereof
KR102515727B1 (ko) 중첩된 가이드핵산을 이용한 표적 핵산에 특정 핵산 서열을 삽입하기 위한 조성물 및 방법
KR102679001B1 (ko) 신규의 개량된 염기 편집 또는 교정용 융합단백질 및 이의 용도
WO2001061030A3 (en) LIBRARIES OF OPTIMUM SUBSEQUENCE REGIONS OF mRNA AND GENOMIC DNA FOR CONTROL OF GENE EXPRESSION
EP4230738A1 (de) Prime-editierungsbasierte geneditierungszusammensetzung mit verbesserter editierungseffizienz und verwendung davon
EP1900815A3 (de) In-vivo-Herstellung kleiner interferierender und Genverstummung vermittelnder RNAs
MXPA02004782A (es) Genes del empequenecimiento del sorgo y metodos de uso.
KR20190023602A (ko) 생식조절인자의 유전적 조작 및 이의 용도
EP0777730A4 (de) Dna-vektor für knochenspezifische genexpression
Erickson Antisense Approaches for Studying Growth and Development

Legal Events

Date Code Title Description
R082 Change of representative

Ref document number: 1210123

Country of ref document: EP

Representative=s name: ZIMMERMANN & PARTNER, 80331 MUENCHEN, DE