DE60034315T2 - Chemisches und biochemisches nachweisverfahren und vorrichtung - Google Patents
Chemisches und biochemisches nachweisverfahren und vorrichtung Download PDFInfo
- Publication number
- DE60034315T2 DE60034315T2 DE60034315T DE60034315T DE60034315T2 DE 60034315 T2 DE60034315 T2 DE 60034315T2 DE 60034315 T DE60034315 T DE 60034315T DE 60034315 T DE60034315 T DE 60034315T DE 60034315 T2 DE60034315 T2 DE 60034315T2
- Authority
- DE
- Germany
- Prior art keywords
- bead
- solution
- component
- beads
- intensity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001514 detection method Methods 0.000 title claims description 12
- 239000000126 substance Substances 0.000 title abstract description 4
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- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 9
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 8
- 238000004364 calculation method Methods 0.000 description 7
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- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
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- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
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- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 3
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 108010001857 Cell Surface Receptors Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
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- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
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- 201000010099 disease Diseases 0.000 description 2
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- 206010001497 Agitation Diseases 0.000 description 1
- 241001136792 Alle Species 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
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- 239000012491 analyte Substances 0.000 description 1
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- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
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- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
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- 239000000356 contaminant Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
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- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
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- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 238000000670 ligand binding assay Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
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- 239000008363 phosphate buffer Substances 0.000 description 1
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- 229920002223 polystyrene Polymers 0.000 description 1
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- 239000012857 radioactive material Substances 0.000 description 1
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- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
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- 238000005728 strengthening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- 238000004448 titration Methods 0.000 description 1
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- 230000007704 transition Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/806—Electrical property or magnetic property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/15—Inorganic acid or base [e.g., hcl, sulfuric acid, etc. ]
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9903555 | 1999-02-16 | ||
| GBGB9903555.2A GB9903555D0 (en) | 1999-02-16 | 1999-02-16 | Chemical and biological assay method and apparatus |
| PCT/GB2000/000549 WO2000049415A1 (en) | 1999-02-16 | 2000-02-16 | Chemical and biochemical assay method and apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60034315D1 DE60034315D1 (de) | 2007-05-24 |
| DE60034315T2 true DE60034315T2 (de) | 2008-01-03 |
Family
ID=10847903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60034315T Expired - Lifetime DE60034315T2 (de) | 1999-02-16 | 2000-02-16 | Chemisches und biochemisches nachweisverfahren und vorrichtung |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6730521B1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1153299B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP4527884B2 (cg-RX-API-DMAC7.html) |
| AT (1) | ATE359517T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU2561400A (cg-RX-API-DMAC7.html) |
| DE (1) | DE60034315T2 (cg-RX-API-DMAC7.html) |
| GB (1) | GB9903555D0 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2000049415A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1239284A1 (en) * | 2001-03-08 | 2002-09-11 | The Technology Partnership Public Limited Company | Non-separation assay method and system using opaque particles |
| WO2003096015A1 (en) * | 2002-05-08 | 2003-11-20 | Matsushita Electric Industrial Co., Ltd. | Biomolecular substrate, method of testing or diagnosis with use thereof and apparatus therefor |
| US7368082B1 (en) * | 2002-12-12 | 2008-05-06 | Tung-Lian Huang | Formulation of spotting solution to achieve uniform spot size and morphology and for nondestructive quality control of assay articles |
| US20040171167A1 (en) * | 2002-12-13 | 2004-09-02 | Affymetrix, Inc. | Chip-in-a-well scanning |
| US7092843B2 (en) * | 2003-10-21 | 2006-08-15 | X-Ray Optical Systems, Inc. | Apparatus and method for suppressing insignificant variations in measured sample composition data, including data measured from dynamically changing samples using x-ray analysis techniques |
| US7877212B2 (en) * | 2004-08-04 | 2011-01-25 | Agilent Technologies, Inc. | Methods and compositions for assessing partially saturated pixel signals |
| US8735142B2 (en) * | 2005-08-16 | 2014-05-27 | Chipotle Business Group, Inc. | Systems and methods for immunosorbent assays for single and multiple analytes |
| US7767404B2 (en) * | 2005-08-16 | 2010-08-03 | Chipotle Business Group, Inc. | Apparatus and method for single-step immunosorbent assay for single and multiple analytes |
| EP4071458B1 (en) | 2005-09-21 | 2025-11-05 | Luminex Corporation | Methods and systems for image data processing |
| CN102016686B (zh) | 2008-03-21 | 2015-03-25 | 艾博特健康公司 | 确定对生物样本成像的成像装置的焦点位置的方法和设备 |
| EP2265945B1 (en) * | 2008-03-21 | 2012-11-07 | Abbott Point Of Care, Inc. | Method and apparatus for determining red blood cell indices of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells |
| ES2588223T3 (es) | 2008-03-21 | 2016-10-31 | Abbott Point Of Care, Inc. | Procedimiento para el análisis de células individuales o partículas en la sangre usando fluorescencia y absorción de un colorante |
| CN102016578B (zh) | 2008-03-21 | 2014-10-01 | 艾博特健康公司 | 利用红细胞内含有的血红蛋白的本征色素沉着来确定血样的血细胞比容的方法及设备 |
| ES2560098T3 (es) * | 2008-03-21 | 2016-02-17 | Abbott Point Of Care, Inc. | Método y aparato para detectar y contar plaquetas individualmente y en agregados |
| WO2009124179A1 (en) | 2008-04-02 | 2009-10-08 | Abbott Point Of Care, Inc. | Virtual separation of bound and free label in a ligand assay for performing immunoassays of biological fluids, including whole blood |
| WO2009126800A1 (en) * | 2008-04-09 | 2009-10-15 | Abbott Point Of Care, Inc. | Method for measuring the area of a sample disposed within an analysis chamber |
| WO2009126505A1 (en) * | 2008-04-09 | 2009-10-15 | Abbott Point Of Care, Inc. | Method of detecting very low levels of analyte within a thin film fluid sample contained in a thin thickness chamber |
| US20100255605A1 (en) * | 2009-04-02 | 2010-10-07 | Abbott Point Of Care, Inc. | Method and device for transferring biologic fluid samples |
| ES2438841T3 (es) * | 2009-12-31 | 2014-01-20 | Abbott Point Of Care, Inc. | Método y aparato para determinar el volumen celular medio de los glóbulos rojos en la sangre |
| US8472693B2 (en) | 2010-03-18 | 2013-06-25 | Abbott Point Of Care, Inc. | Method for determining at least one hemoglobin related parameter of a whole blood sample |
| WO2011119441A1 (en) | 2010-03-22 | 2011-09-29 | Bionex Solutions Inc. | Transfer or interrogation of materials by carrier and receiving devices moving independently and simultaneously on multiple axes |
| JP5433517B2 (ja) * | 2010-07-14 | 2014-03-05 | 株式会社日立ハイテクノロジーズ | 解析装置及び解析方法 |
| US10190986B2 (en) | 2011-06-06 | 2019-01-29 | Abbott Laboratories | Spatially resolved ligand-receptor binding assays |
| EP2769198B1 (en) | 2011-10-18 | 2016-05-25 | Luminex Corporation | Methods and systems for image data processing |
| CN110312923A (zh) * | 2017-03-14 | 2019-10-08 | 凸版印刷株式会社 | 解析设备、解析试剂盒及解析系统 |
| EP3830577B1 (en) * | 2018-08-03 | 2023-06-07 | InSingulo AB | A method for determining the interaction between a ligand and a receptor |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4816419A (en) * | 1983-08-01 | 1989-03-28 | University Of Health Sciences/The Chicago Medical School | Fluorescence ligand binding assay using charge-matched dye and solvent components |
| US5830769A (en) * | 1985-03-18 | 1998-11-03 | Wieder; Irwin | Homogeneous fluorassay methods employing fluorescent background rejection and water-soluble rare earth metal chelates |
| GB8827853D0 (en) * | 1988-11-29 | 1988-12-29 | Ares Serono Res & Dev Ltd | Sensor for optical assay |
| JP3211389B2 (ja) * | 1992-06-30 | 2001-09-25 | 株式会社島津製作所 | 蛍光検出型ゲル電気泳動装置 |
| GB9326450D0 (en) * | 1993-12-24 | 1994-02-23 | Multilyte Ltd | Binding assay |
| DE69417899T2 (de) * | 1994-11-17 | 1999-11-04 | Chemunex, Maisons-Alfort | Vorrichtung und Verfahren zum Erkennen und Zählen von selten vorkommenden Säugerzellen |
| EP0713087B1 (en) * | 1994-11-17 | 1999-04-14 | Chemunex | Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence |
-
1999
- 1999-02-16 GB GBGB9903555.2A patent/GB9903555D0/en not_active Ceased
-
2000
- 2000-02-16 EP EP00903860A patent/EP1153299B1/en not_active Expired - Lifetime
- 2000-02-16 US US09/913,630 patent/US6730521B1/en not_active Expired - Lifetime
- 2000-02-16 AT AT00903860T patent/ATE359517T1/de not_active IP Right Cessation
- 2000-02-16 WO PCT/GB2000/000549 patent/WO2000049415A1/en not_active Ceased
- 2000-02-16 AU AU25614/00A patent/AU2561400A/en not_active Abandoned
- 2000-02-16 DE DE60034315T patent/DE60034315T2/de not_active Expired - Lifetime
- 2000-02-16 JP JP2000600105A patent/JP4527884B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002537563A (ja) | 2002-11-05 |
| JP4527884B2 (ja) | 2010-08-18 |
| DE60034315D1 (de) | 2007-05-24 |
| EP1153299A1 (en) | 2001-11-14 |
| WO2000049415A1 (en) | 2000-08-24 |
| EP1153299B1 (en) | 2007-04-11 |
| AU2561400A (en) | 2000-09-04 |
| US6730521B1 (en) | 2004-05-04 |
| ATE359517T1 (de) | 2007-05-15 |
| GB9903555D0 (en) | 1999-04-07 |
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