JP4527884B2 - 化学的及び生物化学的アッセイ方法及び装置 - Google Patents
化学的及び生物化学的アッセイ方法及び装置 Download PDFInfo
- Publication number
- JP4527884B2 JP4527884B2 JP2000600105A JP2000600105A JP4527884B2 JP 4527884 B2 JP4527884 B2 JP 4527884B2 JP 2000600105 A JP2000600105 A JP 2000600105A JP 2000600105 A JP2000600105 A JP 2000600105A JP 4527884 B2 JP4527884 B2 JP 4527884B2
- Authority
- JP
- Japan
- Prior art keywords
- component
- beads
- solution
- amount
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 87
- 239000000126 substance Substances 0.000 title abstract description 6
- 238000010256 biochemical assay Methods 0.000 title abstract description 4
- 238000009739 binding Methods 0.000 claims abstract description 73
- 230000027455 binding Effects 0.000 claims abstract description 71
- 238000003556 assay Methods 0.000 claims abstract description 66
- 150000001875 compounds Chemical class 0.000 claims abstract description 16
- 238000000926 separation method Methods 0.000 claims abstract description 8
- 230000008859 change Effects 0.000 claims abstract description 5
- 239000011324 bead Substances 0.000 claims description 144
- 238000005259 measurement Methods 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 22
- 230000005499 meniscus Effects 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 12
- 238000002372 labelling Methods 0.000 claims description 11
- 238000010494 dissociation reaction Methods 0.000 claims description 10
- 230000005593 dissociations Effects 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 6
- 230000006957 competitive inhibition Effects 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 4
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 239000000356 contaminant Substances 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 238000013537 high throughput screening Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 abstract 3
- 239000007787 solid Substances 0.000 abstract 3
- 238000004624 confocal microscopy Methods 0.000 abstract 1
- 238000007405 data analysis Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 66
- 239000000243 solution Substances 0.000 description 57
- 239000003446 ligand Substances 0.000 description 53
- 102000005962 receptors Human genes 0.000 description 43
- 108020003175 receptors Proteins 0.000 description 43
- 239000000523 sample Substances 0.000 description 38
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 33
- 230000008569 process Effects 0.000 description 20
- 238000001444 catalytic combustion detection Methods 0.000 description 12
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 8
- 238000011109 contamination Methods 0.000 description 7
- 238000009826 distribution Methods 0.000 description 7
- 238000007876 drug discovery Methods 0.000 description 7
- 238000002060 fluorescence correlation spectroscopy Methods 0.000 description 7
- 230000003993 interaction Effects 0.000 description 7
- 238000013178 mathematical model Methods 0.000 description 7
- 239000002953 phosphate buffered saline Substances 0.000 description 7
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 239000002547 new drug Substances 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 238000005070 sampling Methods 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 238000012937 correction Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 5
- 238000002821 scintillation proximity assay Methods 0.000 description 5
- 230000003595 spectral effect Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000002875 fluorescence polarization Methods 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 238000010791 quenching Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000003491 array Methods 0.000 description 3
- 238000003759 clinical diagnosis Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 3
- 230000009871 nonspecific binding Effects 0.000 description 3
- 230000010287 polarization Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 230000032258 transport Effects 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 108010001857 Cell Surface Receptors Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012875 competitive assay Methods 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 102000006240 membrane receptors Human genes 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 230000005653 Brownian motion process Effects 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 240000002426 Persea americana var. drymifolia Species 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000007488 abnormal function Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 238000005537 brownian motion Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011362 coarse particle Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000008606 intracellular interaction Effects 0.000 description 1
- 102000027411 intracellular receptors Human genes 0.000 description 1
- 108091008582 intracellular receptors Proteins 0.000 description 1
- 238000000670 ligand binding assay Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 239000012857 radioactive material Substances 0.000 description 1
- 238000001525 receptor binding assay Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008672 reprogramming Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- -1 such as a cell Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000000954 titration curve Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/806—Electrical property or magnetic property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/15—Inorganic acid or base [e.g., hcl, sulfuric acid, etc. ]
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9903555.2 | 1999-02-16 | ||
| GBGB9903555.2A GB9903555D0 (en) | 1999-02-16 | 1999-02-16 | Chemical and biological assay method and apparatus |
| PCT/GB2000/000549 WO2000049415A1 (en) | 1999-02-16 | 2000-02-16 | Chemical and biochemical assay method and apparatus |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002537563A JP2002537563A (ja) | 2002-11-05 |
| JP2002537563A5 JP2002537563A5 (cg-RX-API-DMAC7.html) | 2007-05-17 |
| JP4527884B2 true JP4527884B2 (ja) | 2010-08-18 |
Family
ID=10847903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000600105A Expired - Fee Related JP4527884B2 (ja) | 1999-02-16 | 2000-02-16 | 化学的及び生物化学的アッセイ方法及び装置 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6730521B1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1153299B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP4527884B2 (cg-RX-API-DMAC7.html) |
| AT (1) | ATE359517T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU2561400A (cg-RX-API-DMAC7.html) |
| DE (1) | DE60034315T2 (cg-RX-API-DMAC7.html) |
| GB (1) | GB9903555D0 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2000049415A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1239284A1 (en) * | 2001-03-08 | 2002-09-11 | The Technology Partnership Public Limited Company | Non-separation assay method and system using opaque particles |
| WO2003096015A1 (en) * | 2002-05-08 | 2003-11-20 | Matsushita Electric Industrial Co., Ltd. | Biomolecular substrate, method of testing or diagnosis with use thereof and apparatus therefor |
| US7368082B1 (en) * | 2002-12-12 | 2008-05-06 | Tung-Lian Huang | Formulation of spotting solution to achieve uniform spot size and morphology and for nondestructive quality control of assay articles |
| US20040171167A1 (en) * | 2002-12-13 | 2004-09-02 | Affymetrix, Inc. | Chip-in-a-well scanning |
| US7092843B2 (en) * | 2003-10-21 | 2006-08-15 | X-Ray Optical Systems, Inc. | Apparatus and method for suppressing insignificant variations in measured sample composition data, including data measured from dynamically changing samples using x-ray analysis techniques |
| US7877212B2 (en) * | 2004-08-04 | 2011-01-25 | Agilent Technologies, Inc. | Methods and compositions for assessing partially saturated pixel signals |
| US8735142B2 (en) * | 2005-08-16 | 2014-05-27 | Chipotle Business Group, Inc. | Systems and methods for immunosorbent assays for single and multiple analytes |
| US7767404B2 (en) * | 2005-08-16 | 2010-08-03 | Chipotle Business Group, Inc. | Apparatus and method for single-step immunosorbent assay for single and multiple analytes |
| EP4071458B1 (en) | 2005-09-21 | 2025-11-05 | Luminex Corporation | Methods and systems for image data processing |
| CN102016686B (zh) | 2008-03-21 | 2015-03-25 | 艾博特健康公司 | 确定对生物样本成像的成像装置的焦点位置的方法和设备 |
| EP2265945B1 (en) * | 2008-03-21 | 2012-11-07 | Abbott Point Of Care, Inc. | Method and apparatus for determining red blood cell indices of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells |
| ES2588223T3 (es) | 2008-03-21 | 2016-10-31 | Abbott Point Of Care, Inc. | Procedimiento para el análisis de células individuales o partículas en la sangre usando fluorescencia y absorción de un colorante |
| CN102016578B (zh) | 2008-03-21 | 2014-10-01 | 艾博特健康公司 | 利用红细胞内含有的血红蛋白的本征色素沉着来确定血样的血细胞比容的方法及设备 |
| ES2560098T3 (es) * | 2008-03-21 | 2016-02-17 | Abbott Point Of Care, Inc. | Método y aparato para detectar y contar plaquetas individualmente y en agregados |
| WO2009124179A1 (en) | 2008-04-02 | 2009-10-08 | Abbott Point Of Care, Inc. | Virtual separation of bound and free label in a ligand assay for performing immunoassays of biological fluids, including whole blood |
| WO2009126800A1 (en) * | 2008-04-09 | 2009-10-15 | Abbott Point Of Care, Inc. | Method for measuring the area of a sample disposed within an analysis chamber |
| WO2009126505A1 (en) * | 2008-04-09 | 2009-10-15 | Abbott Point Of Care, Inc. | Method of detecting very low levels of analyte within a thin film fluid sample contained in a thin thickness chamber |
| US20100255605A1 (en) * | 2009-04-02 | 2010-10-07 | Abbott Point Of Care, Inc. | Method and device for transferring biologic fluid samples |
| ES2438841T3 (es) * | 2009-12-31 | 2014-01-20 | Abbott Point Of Care, Inc. | Método y aparato para determinar el volumen celular medio de los glóbulos rojos en la sangre |
| US8472693B2 (en) | 2010-03-18 | 2013-06-25 | Abbott Point Of Care, Inc. | Method for determining at least one hemoglobin related parameter of a whole blood sample |
| WO2011119441A1 (en) | 2010-03-22 | 2011-09-29 | Bionex Solutions Inc. | Transfer or interrogation of materials by carrier and receiving devices moving independently and simultaneously on multiple axes |
| JP5433517B2 (ja) * | 2010-07-14 | 2014-03-05 | 株式会社日立ハイテクノロジーズ | 解析装置及び解析方法 |
| US10190986B2 (en) | 2011-06-06 | 2019-01-29 | Abbott Laboratories | Spatially resolved ligand-receptor binding assays |
| EP2769198B1 (en) | 2011-10-18 | 2016-05-25 | Luminex Corporation | Methods and systems for image data processing |
| CN110312923A (zh) * | 2017-03-14 | 2019-10-08 | 凸版印刷株式会社 | 解析设备、解析试剂盒及解析系统 |
| EP3830577B1 (en) * | 2018-08-03 | 2023-06-07 | InSingulo AB | A method for determining the interaction between a ligand and a receptor |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4816419A (en) * | 1983-08-01 | 1989-03-28 | University Of Health Sciences/The Chicago Medical School | Fluorescence ligand binding assay using charge-matched dye and solvent components |
| US5830769A (en) * | 1985-03-18 | 1998-11-03 | Wieder; Irwin | Homogeneous fluorassay methods employing fluorescent background rejection and water-soluble rare earth metal chelates |
| GB8827853D0 (en) * | 1988-11-29 | 1988-12-29 | Ares Serono Res & Dev Ltd | Sensor for optical assay |
| JP3211389B2 (ja) * | 1992-06-30 | 2001-09-25 | 株式会社島津製作所 | 蛍光検出型ゲル電気泳動装置 |
| GB9326450D0 (en) * | 1993-12-24 | 1994-02-23 | Multilyte Ltd | Binding assay |
| DE69417899T2 (de) * | 1994-11-17 | 1999-11-04 | Chemunex, Maisons-Alfort | Vorrichtung und Verfahren zum Erkennen und Zählen von selten vorkommenden Säugerzellen |
| EP0713087B1 (en) * | 1994-11-17 | 1999-04-14 | Chemunex | Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence |
-
1999
- 1999-02-16 GB GBGB9903555.2A patent/GB9903555D0/en not_active Ceased
-
2000
- 2000-02-16 EP EP00903860A patent/EP1153299B1/en not_active Expired - Lifetime
- 2000-02-16 US US09/913,630 patent/US6730521B1/en not_active Expired - Lifetime
- 2000-02-16 AT AT00903860T patent/ATE359517T1/de not_active IP Right Cessation
- 2000-02-16 WO PCT/GB2000/000549 patent/WO2000049415A1/en not_active Ceased
- 2000-02-16 AU AU25614/00A patent/AU2561400A/en not_active Abandoned
- 2000-02-16 DE DE60034315T patent/DE60034315T2/de not_active Expired - Lifetime
- 2000-02-16 JP JP2000600105A patent/JP4527884B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002537563A (ja) | 2002-11-05 |
| DE60034315D1 (de) | 2007-05-24 |
| EP1153299A1 (en) | 2001-11-14 |
| WO2000049415A1 (en) | 2000-08-24 |
| EP1153299B1 (en) | 2007-04-11 |
| AU2561400A (en) | 2000-09-04 |
| US6730521B1 (en) | 2004-05-04 |
| ATE359517T1 (de) | 2007-05-15 |
| DE60034315T2 (de) | 2008-01-03 |
| GB9903555D0 (en) | 1999-04-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6730521B1 (en) | Chemical and biochemical assay method and apparatus | |
| US20240377397A1 (en) | Methods and systems for extending dynamic range in assays for the detection of molecules or particles | |
| US20240310367A1 (en) | Compositions and methods for the detection and molecular profiling of membrane bound vesicles | |
| US9551663B2 (en) | Methods and systems for extending dynamic range in assays for the detection of molecules or particles | |
| CN107121396B (zh) | 用于检测分析物的方法 | |
| TW412637B (en) | Optical quantification of analytes in membranes | |
| CA2458802C (en) | Rapid and sensitive detection of molecules | |
| US20060170918A1 (en) | Detection Apparatus and Detection Method for Plasmon Resonance and Fluorescence | |
| US20100196914A1 (en) | Rare cell detection using flat-panel imager and chemiluminescent or radioisotopic tags | |
| KR20120132668A (ko) | 라만 분석 기반 고속 다중 약물 고속 스크리닝 장치 | |
| JP2002526743A (ja) | アナライト検出算出法 | |
| JP2004505245A (ja) | 空間解像型酵素結合アッセイ | |
| JP3340715B2 (ja) | 被検体、その相互作用又は反応動力学を定量的又は定性的に測定する方法及びサンプルキャリア | |
| CN117388227A (zh) | 使用检测微球测定样品中目标分子浓度的方法、计算机可读介质和分析设备 | |
| EP1239284A1 (en) | Non-separation assay method and system using opaque particles | |
| JPH01501893A (ja) | 細胞スクリーニング、装置および方法 | |
| CN114217073A (zh) | 一种基于数字等离子免疫吸附测定法的蛋白质结合动力学超灵敏动态成像分析方法 | |
| JPH11183381A (ja) | 蛍光顕微鏡評価方法および装置 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20040721 |
|
| A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20040721 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20040721 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070214 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070222 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20091208 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20100308 |
|
| RD02 | Notification of acceptance of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7422 Effective date: 20100308 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20100308 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20100331 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100407 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20100511 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20100604 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130611 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| LAPS | Cancellation because of no payment of annual fees |