DE507591T1 - Anionenaustauschtrennung von nukleinsaeuren. - Google Patents
Anionenaustauschtrennung von nukleinsaeuren.Info
- Publication number
- DE507591T1 DE507591T1 DE199292302930T DE92302930T DE507591T1 DE 507591 T1 DE507591 T1 DE 507591T1 DE 199292302930 T DE199292302930 T DE 199292302930T DE 92302930 T DE92302930 T DE 92302930T DE 507591 T1 DE507591 T1 DE 507591T1
- Authority
- DE
- Germany
- Prior art keywords
- solid
- solvent
- concentration
- contacting
- anion exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004707 nucleic acids Proteins 0.000 title claims 8
- 102000039446 nucleic acids Human genes 0.000 title claims 8
- 150000007523 nucleic acids Chemical class 0.000 title claims 8
- 238000005349 anion exchange Methods 0.000 title claims 3
- 239000007787 solid Substances 0.000 claims abstract 15
- 238000000034 method Methods 0.000 claims abstract 8
- 108020004414 DNA Proteins 0.000 claims abstract 5
- 102000053602 DNA Human genes 0.000 claims abstract 5
- 239000002904 solvent Substances 0.000 claims 9
- 239000002253 acid Substances 0.000 claims 3
- 239000000203 mixture Substances 0.000 claims 3
- 150000003839 salts Chemical class 0.000 claims 3
- 239000002245 particle Substances 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 claims 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims 1
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims 1
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 claims 1
- 229910002651 NO3 Inorganic materials 0.000 claims 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims 1
- 229920002873 Polyethylenimine Chemical group 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- 238000002835 absorbance Methods 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 239000003125 aqueous solvent Substances 0.000 claims 1
- 125000002091 cationic group Chemical group 0.000 claims 1
- 150000001768 cations Chemical class 0.000 claims 1
- 125000005131 dialkylammonium group Chemical group 0.000 claims 1
- -1 diethylaminoethyl functional groups Chemical group 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 claims 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- 125000000524 functional group Chemical group 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 125000005207 tetraalkylammonium group Chemical group 0.000 claims 1
- 125000005208 trialkylammonium group Chemical group 0.000 claims 1
- 239000000463 material Substances 0.000 abstract 3
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 150000003974 aralkylamines Chemical class 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67973691A | 1991-04-03 | 1991-04-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE507591T1 true DE507591T1 (de) | 1993-03-18 |
Family
ID=24728140
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE199292302930T Pending DE507591T1 (de) | 1991-04-03 | 1992-04-03 | Anionenaustauschtrennung von nukleinsaeuren. |
| DE69232677T Expired - Fee Related DE69232677T2 (de) | 1991-04-03 | 1992-04-03 | Verfahren zur Trennung doppelsträngiger DNA von einzelsträngiger DNA oder RNA |
| DE69220132T Expired - Fee Related DE69220132T2 (de) | 1991-04-03 | 1992-04-03 | Anionenaustauschtrennung von Nukleinsäuren |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69232677T Expired - Fee Related DE69232677T2 (de) | 1991-04-03 | 1992-04-03 | Verfahren zur Trennung doppelsträngiger DNA von einzelsträngiger DNA oder RNA |
| DE69220132T Expired - Fee Related DE69220132T2 (de) | 1991-04-03 | 1992-04-03 | Anionenaustauschtrennung von Nukleinsäuren |
Country Status (12)
| Country | Link |
|---|---|
| EP (2) | EP0691148B1 (enExample) |
| JP (1) | JP3195039B2 (enExample) |
| KR (1) | KR920019931A (enExample) |
| AT (2) | ATE154132T1 (enExample) |
| AU (1) | AU645674B2 (enExample) |
| CA (1) | CA2063855C (enExample) |
| DE (3) | DE507591T1 (enExample) |
| ES (1) | ES2053416T1 (enExample) |
| GR (1) | GR930300019T1 (enExample) |
| IE (1) | IE921074A1 (enExample) |
| IL (1) | IL101356A (enExample) |
| NZ (1) | NZ242226A (enExample) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT398973B (de) * | 1992-11-18 | 1995-02-27 | Bonn Guenther Dr | Verfahren zur trennung von nukleinsäuren |
| US5622822A (en) * | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
| US5630924A (en) | 1995-04-20 | 1997-05-20 | Perseptive Biosystems, Inc. | Compositions, methods and apparatus for ultrafast electroseparation analysis |
| JP2965131B2 (ja) | 1995-07-07 | 1999-10-18 | 東洋紡績株式会社 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
| US6066258A (en) | 1997-12-05 | 2000-05-23 | Transgenomic, Inc. | Polynucleotide separations on polymeric separation media |
| US5772889A (en) * | 1995-11-13 | 1998-06-30 | Transgenomic, Inc. | System and method for performing nucleic acid separations using liquid chromatography |
| US6355791B1 (en) | 1995-11-13 | 2002-03-12 | Transgenomic, Inc. | Polynucleotide separations on polymeric separation media |
| EP0880537B1 (en) | 1996-02-14 | 2004-12-15 | bioMerieux B.V. | Isolation of single stranded nucleic acids |
| SE9600796D0 (sv) * | 1996-02-29 | 1996-02-29 | Pharmacia Biotech Ab | A method of preparing a liquid mixture |
| US5997742A (en) * | 1996-11-13 | 1999-12-07 | Transgenomic, Inc. | Method for performing polynucleotide separations using liquid chromatography |
| US6017457A (en) * | 1996-11-13 | 2000-01-25 | Transgenomic, Inc. | Method for performing polynucleotide separations using liquid chromatography |
| US6475388B1 (en) | 1996-11-13 | 2002-11-05 | Transgenomic, Inc. | Method and system for RNA analysis by matched ion polynucleotide chromatography |
| US6471866B1 (en) | 1996-11-13 | 2002-10-29 | Transgenomic, Inc. | Process for performing polynucleotide separations |
| US6174441B1 (en) | 1996-11-13 | 2001-01-16 | Transgenomic, Inc. | Method for performing polynucleotide separations using liquid chromatography |
| US6576133B2 (en) | 1996-11-13 | 2003-06-10 | Transgenomic, Inc | Method and system for RNA analysis by matched ion polynucleotide chromatography |
| US5972222A (en) * | 1996-11-13 | 1999-10-26 | Transgenomic, Inc. | Process for performing polynucleotide separations |
| US7138518B1 (en) | 1996-11-13 | 2006-11-21 | Transgenomic, Inc. | Liquid chromatographic separation of polynucleotides |
| US6482317B2 (en) | 1996-11-13 | 2002-11-19 | Transgenomic, Inc. | Polynucleotide separations on polymeric separation media |
| US6287822B1 (en) | 1997-08-05 | 2001-09-11 | Transgenomic, Inc. | Mutation detection method |
| US6030527A (en) * | 1996-11-13 | 2000-02-29 | Transgenomic, Inc. | Apparatus for performing polynucleotide separations using liquid chromatography |
| US6372142B1 (en) | 1996-11-13 | 2002-04-16 | Transgenomic, Inc. | Column for DNA separation by matched ion polynucleotide chromatography |
| US6177559B1 (en) | 1998-04-24 | 2001-01-23 | Transgenomic, Inc. | Process for separation of polynucleotide fragments |
| US5986085A (en) * | 1997-04-25 | 1999-11-16 | Transgenomic, Inc. | Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments |
| US6056877A (en) * | 1997-12-05 | 2000-05-02 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
| DE69817207T2 (de) | 1997-06-10 | 2004-06-17 | Transgenomic, Inc., San Jose | System und verfahren zur durchführung von polynukleotid-trennungen mit hilfe von flüssigchromatographie |
| CA2268368A1 (en) * | 1997-08-28 | 1999-03-11 | The Perkin-Elmer Corporation | Improved detection of mutations in nucleic acids by chemical cleavage |
| US6372130B1 (en) | 1997-12-05 | 2002-04-16 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
| US6258264B1 (en) | 1998-04-10 | 2001-07-10 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
| US6503397B2 (en) | 1997-12-05 | 2003-01-07 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
| US6265168B1 (en) | 1998-10-06 | 2001-07-24 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
| US6485648B1 (en) | 1998-05-18 | 2002-11-26 | Transgenomic, Inc. | MIPC column cleaning system and process |
| US6455692B1 (en) | 1998-08-04 | 2002-09-24 | Transgenomic, Inc. | Method of concentrating polynucleotides using MIPC |
| US6074880A (en) * | 1998-08-28 | 2000-06-13 | Transgenomic, Inc. | Sample analyte containing solution fraction collection system, and method of use |
| WO2001027331A2 (en) * | 1999-10-12 | 2001-04-19 | Transgenomic, Inc. | Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography |
| WO2001055713A2 (en) | 2000-01-26 | 2001-08-02 | Transgenomic, Inc. | Method and apparatus for separating polynucleotides using monolithic capillary columns |
| US6969587B2 (en) | 2000-04-04 | 2005-11-29 | Taylor Paul D | Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography |
| DE60119173T2 (de) * | 2000-08-30 | 2007-01-18 | Avi Biopharma, Inc., Corvallis | Methode zur analyse von oligonukleotidanaloga |
| US20020197726A1 (en) * | 2001-05-23 | 2002-12-26 | Gyula Vigh | Anionic ion pairing compositions having volatile combustion products with O or S or halogen atoms or their combinations as heteroatoms for use with gas-phase or vapor-phase element-specific detectors |
| US20030004371A1 (en) * | 2001-05-23 | 2003-01-02 | Gyula Vigh | Cationic ion pairing compositions having volatile combustion products with O or S or halogen atoms or their combinations as heteroatoms for use with gas-phase or vapor-phase element-specific detectors |
| AU2003256787A1 (en) * | 2002-07-26 | 2004-02-16 | Applera Corporation | Petal-array support for use with microplates |
| DE102006020463B4 (de) * | 2005-05-24 | 2013-05-23 | Sartorius Stedim Biotech Gmbh | Verfahren zum Entfernen von Nukleinsäuren aus einer Flüssigkeit, Verfahren zur Herstellung von Adsorberelementen und Verwendung von Membranadsorbermaterial-Verschnitt |
| AU2012205204B2 (en) * | 2005-12-09 | 2015-03-12 | Qiagen Gmbh | Method for enriching short-chain nucleic acids |
| DE102005059315A1 (de) * | 2005-12-09 | 2007-06-14 | Qiagen Gmbh | Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren |
| CA2668818C (en) | 2006-10-10 | 2018-06-26 | Xenomics, Inc. | Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media |
| ES2613740T3 (es) | 2009-02-11 | 2017-05-25 | University Of Rochester | Medios porosos funcionalizados de forma espacialmente no homogénea y procedimiento para su uso en la eliminación selectiva de contaminantes |
| JP5914338B2 (ja) * | 2009-09-24 | 2016-05-11 | キアジェン ゲイサーズバーグ インコーポレイテッド | 陰イオン交換材料を使用した核酸の単離および分析のための組成物、方法およびキット |
| JP6061381B2 (ja) | 2011-01-12 | 2017-01-18 | 積水メディカル株式会社 | 一塩基多型の検出方法 |
| US9481881B2 (en) | 2011-01-12 | 2016-11-01 | Sekisui Medical Co., Ltd. | Eluent for ion-exchange chromatography, and method of analyzing nucleic acid chains |
| JPWO2012133834A1 (ja) * | 2011-03-31 | 2014-07-28 | 積水メディカル株式会社 | 一塩基多型検出用の試料核酸、一塩基多型検出試料調製用のｐｃｒプライマー及びイオン交換クロマトグラフィー分析に用いる一塩基多型検出用試料の調製方法 |
| CN106380592B (zh) * | 2016-11-04 | 2018-10-16 | 江南大学 | 一种从发酵液中提取ε-聚赖氨酸及其盐酸盐的方法 |
| CN111511924A (zh) * | 2017-11-08 | 2020-08-07 | 库瑞瓦格股份公司 | Rna序列调整 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4672040A (en) * | 1983-05-12 | 1987-06-09 | Advanced Magnetics, Inc. | Magnetic particles for use in separations |
| DE3639949A1 (de) * | 1986-11-22 | 1988-06-09 | Diagen Inst Molekularbio | Verfahren zur trennung von langkettigen nukleinsaeuren |
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| DE3717211A1 (de) * | 1987-05-22 | 1988-12-01 | Diagen Inst Molekularbio | Vorrichtung und verfahren zur trennung und reinigung von molekuelen |
| US4959176A (en) * | 1988-04-29 | 1990-09-25 | Beckman Instruments, Inc. | High resolution non corrosive amino acid analysis buffer solutions |
| SE8902315D0 (sv) * | 1989-06-27 | 1989-06-27 | Pharmacia Ab | Anjonbytare |
| US5010183A (en) * | 1989-07-07 | 1991-04-23 | Macfarlane Donald E | Process for purifying DNA and RNA using cationic detergents |
-
1992
- 1992-03-24 CA CA002063855A patent/CA2063855C/en not_active Expired - Fee Related
- 1992-03-24 IL IL10135692A patent/IL101356A/en not_active IP Right Cessation
- 1992-04-01 JP JP07964392A patent/JP3195039B2/ja not_active Expired - Fee Related
- 1992-04-02 KR KR1019920005492A patent/KR920019931A/ko not_active Withdrawn
- 1992-04-03 IE IE107492A patent/IE921074A1/en unknown
- 1992-04-03 ES ES92302930T patent/ES2053416T1/es active Pending
- 1992-04-03 DE DE199292302930T patent/DE507591T1/de active Pending
- 1992-04-03 AT AT92302930T patent/ATE154132T1/de not_active IP Right Cessation
- 1992-04-03 NZ NZ242226A patent/NZ242226A/en not_active IP Right Cessation
- 1992-04-03 AU AU14034/92A patent/AU645674B2/en not_active Ceased
- 1992-04-03 EP EP95202705A patent/EP0691148B1/en not_active Expired - Lifetime
- 1992-04-03 AT AT95202705T patent/ATE220341T1/de not_active IP Right Cessation
- 1992-04-03 EP EP92302930A patent/EP0507591B1/en not_active Expired - Lifetime
- 1992-04-03 DE DE69232677T patent/DE69232677T2/de not_active Expired - Fee Related
- 1992-04-03 DE DE69220132T patent/DE69220132T2/de not_active Expired - Fee Related
-
1993
- 1993-04-28 GR GR930300019T patent/GR930300019T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL101356A (en) | 1996-08-04 |
| DE69220132D1 (de) | 1997-07-10 |
| IL101356A0 (en) | 1992-11-15 |
| DE69220132T2 (de) | 1997-11-13 |
| CA2063855A1 (en) | 1992-10-04 |
| EP0507591A3 (en) | 1993-02-03 |
| AU645674B2 (en) | 1994-01-20 |
| IE921074A1 (en) | 1992-10-07 |
| GR930300019T1 (en) | 1993-04-28 |
| ES2053416T1 (es) | 1994-08-01 |
| EP0691148A2 (en) | 1996-01-10 |
| EP0691148A3 (enExample) | 1996-01-24 |
| EP0691148B1 (en) | 2002-07-10 |
| ATE220341T1 (de) | 2002-07-15 |
| JP3195039B2 (ja) | 2001-08-06 |
| DE69232677T2 (de) | 2003-02-13 |
| AU1403492A (en) | 1992-10-08 |
| KR920019931A (ko) | 1992-11-20 |
| JPH0599909A (ja) | 1993-04-23 |
| NZ242226A (en) | 1993-07-27 |
| EP0507591B1 (en) | 1997-06-04 |
| DE69232677D1 (de) | 2002-08-14 |
| ATE154132T1 (de) | 1997-06-15 |
| EP0507591A2 (en) | 1992-10-07 |
| CA2063855C (en) | 1997-08-26 |
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