KR920019931A - 핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법 - Google Patents

핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법 Download PDF

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KR920019931A
KR920019931A KR1019920005492A KR920005492A KR920019931A KR 920019931 A KR920019931 A KR 920019931A KR 1019920005492 A KR1019920005492 A KR 1019920005492A KR 920005492 A KR920005492 A KR 920005492A KR 920019931 A KR920019931 A KR 920019931A
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nucleic acids
nucleic acid
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브로치 윌
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더 퍼킨 엘머 코포레이션
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Abstract

내용 없음

Description

핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (19)

  1. (a) (1) 디알킬암모늄, 트리알킬암모늄 및 테트라알킬암모늄(여기에서, 알킬 그룹은 메틸 및 에틸의 배합으로 이루어진다)으로 이루어진 그룹 중에서 선택된 양이온과 (2) 브로마이드, 클로라이드, 아세테이트, 포르메이트, 니트레이트, 피클로레이트, 디하이드로겐 포스페이트, 에탄 설포네이트 및 메탄 설포네이트로 이루어진 그룹 중에서 선택된 음이온의 동일 농도로 구성된 0.5 내지 1.5M의 적당한 농도 범위의 용출염 및 (b) 약 0.05M의 농도를 초과하지 않고 3.5 내지 8.5의 적당한 pKa 범위를 갖는 완충산을 함유하고, 약 4 내지 8의 pH 범위를 가지며, 물에 대하여 260㎚(광로 1㎝)에서의 흡광도가 약 0.1을 초과하지 않는, 핵산의 음이온-교환 크로마토그래피에 유용한 수성용매.
  2. 제 1항에 있어서, 완충산이 양이온이고 양이온이거나 중성인 하전을 갖는 짝염기를 갖는 용매.
  3. 수중 조성물의 1/2 내지 1/20 희석 결과 제 1항의 용매를 제공하도록 제 1항에 따른 용매의 약 2배 내지 20배 농축물을 함유하는 수성 조성물.
  4. 제 1항에 있어서, 약 1㎍/L 내지 1g/L의 농도 범위로 핵산을 추가로 함유하는 조성물.
  5. 제 4항에 있어서, 핵산이 이본쇄 DNA인 조성물.
  6. 제 1항의 용매가 음이온-교환 고체와 배합된 조성물.
  7. 제 6항에 있어서, 고체가 폴리크릴계 골격을 포함하는 조성물.
  8. 제 6항에 있어서, 고체가 디에틸아미노에틸 작용그룹을 포함하는 조성물.
  9. 제 6항에 있어서, 고체가 폴리에틸렌이민 작용그룹을 포함하는 조성물.
  10. 제 6항에 있어서, 고체가 약 10㎛ 내지 2㎛의 평균 직경을 입자를 포함하는 조성물.
  11. 제 6항에 있어서, 고체가 실질적으로 비공성인 조성물.
  12. 제 6항에 있어서, 고체가 폴리스티렌 골격을 포함하는 조성물.
  13. 제 6항에 있어서, 약 1㎍/L 내지 1g/L의 농도 범위의 핵산을 추가로 함유하는 조성물.
  14. (a) 핵산을 함유하는 시험샘플을 음이온-교환고체와 접촉시키고, (b) 단계 (a)의 고체를 일련의 제 1항의 용매(여기에서, 용출염의 농도는 용매의 대부분이 접촉 후 고체로부터 분리되도록 약 0.5M로부터 약 1.5M로 체계적으로 증가된다)와 접촉시키며, (c) 핵산에 대해 일련의 용매를 분석함을 특징으로 하여, 분자 크기의 차이를 근거로 핵산을 분리하고 분석하는 방법.
  15. 제 14항에 있어서, 고체와 접촉하는 용출염의 농도가 연속적으로 증가하는 방법.
  16. 제 14항에 있어서, 고체로부터 분리된 용매 중의 핵산의 농도를 약 250㎚ 내지 290㎚의 파장 범위에서 자외선 흡광도로 측정하는 방법.
  17. 제 14항에 있어서, 고체가 약 10㎛ 내지 약 2㎛의 평균 직경을 갖는 입자를 포함하는 방법.
  18. 제 17항에 있어서, 고체가 직경이 약 1㎜ 내지 약 6㎜이고 길이가 약 10㎜ 내지 약 60㎜인 실린더형 컬럼에 함유된 방법.
  19. 제 14항에 있어서, 공정을 완성하는데 필요한 총 시간이 약 2분 내지 약 30분인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920005492A 1991-04-03 1992-04-02 핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법 KR920019931A (ko)

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EP (2) EP0691148B1 (ko)
JP (1) JP3195039B2 (ko)
KR (1) KR920019931A (ko)
AT (2) ATE220341T1 (ko)
AU (1) AU645674B2 (ko)
CA (1) CA2063855C (ko)
DE (3) DE69220132T2 (ko)
ES (1) ES2053416T1 (ko)
GR (1) GR930300019T1 (ko)
IE (1) IE921074A1 (ko)
IL (1) IL101356A (ko)
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CA2063855A1 (en) 1992-10-04
IL101356A (en) 1996-08-04
EP0691148A3 (ko) 1996-01-24
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DE69232677D1 (de) 2002-08-14
JP3195039B2 (ja) 2001-08-06
DE69220132D1 (de) 1997-07-10
EP0507591A2 (en) 1992-10-07
EP0507591A3 (en) 1993-02-03
DE507591T1 (de) 1993-03-18
ATE220341T1 (de) 2002-07-15
AU1403492A (en) 1992-10-08
GR930300019T1 (en) 1993-04-28
EP0691148B1 (en) 2002-07-10
NZ242226A (en) 1993-07-27
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EP0507591B1 (en) 1997-06-04
EP0691148A2 (en) 1996-01-10
DE69220132T2 (de) 1997-11-13
IE921074A1 (en) 1992-10-07
ATE154132T1 (de) 1997-06-15
CA2063855C (en) 1997-08-26
DE69232677T2 (de) 2003-02-13
IL101356A0 (en) 1992-11-15

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