DE4333805C2 - Verfahren zur Extraktion von Nucleinsäuren und Verfahren zum Nachweis spezifizierter Nucleinsäuresequenzen - Google Patents

Verfahren zur Extraktion von Nucleinsäuren und Verfahren zum Nachweis spezifizierter Nucleinsäuresequenzen

Info

Publication number
DE4333805C2
DE4333805C2 DE19934333805 DE4333805A DE4333805C2 DE 4333805 C2 DE4333805 C2 DE 4333805C2 DE 19934333805 DE19934333805 DE 19934333805 DE 4333805 A DE4333805 A DE 4333805A DE 4333805 C2 DE4333805 C2 DE 4333805C2
Authority
DE
Germany
Prior art keywords
reagent
nucleic acids
pcr
alcohol
carrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE19934333805
Other languages
German (de)
English (en)
Other versions
DE4333805A1 (de
Inventor
Hiroaki Yamagishi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Publication of DE4333805A1 publication Critical patent/DE4333805A1/de
Application granted granted Critical
Publication of DE4333805C2 publication Critical patent/DE4333805C2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE19934333805 1993-08-24 1993-10-04 Verfahren zur Extraktion von Nucleinsäuren und Verfahren zum Nachweis spezifizierter Nucleinsäuresequenzen Expired - Fee Related DE4333805C2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23087093A JP3451667B2 (ja) 1993-08-24 1993-08-24 核酸抽出及び特定核酸配列の検出方法

Publications (2)

Publication Number Publication Date
DE4333805A1 DE4333805A1 (de) 1995-03-02
DE4333805C2 true DE4333805C2 (de) 2003-08-07

Family

ID=16914603

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19934333805 Expired - Fee Related DE4333805C2 (de) 1993-08-24 1993-10-04 Verfahren zur Extraktion von Nucleinsäuren und Verfahren zum Nachweis spezifizierter Nucleinsäuresequenzen

Country Status (2)

Country Link
JP (1) JP3451667B2 (ja)
DE (1) DE4333805C2 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US11739382B2 (en) 2019-08-27 2023-08-29 Roche Molecular Systems, Inc. Compositions and methods for amplification and detection of hepatitis B virus RNA, including HBV RNA transcribed from cccDNA

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3108105B2 (ja) * 1995-08-21 2000-11-13 三光純薬株式会社 共沈剤及び核酸の抽出方法
WO1997012994A1 (en) * 1995-10-02 1997-04-10 Novagen, Inc. Method for precipitating nucleic acid with visible carrier
DE10033583A1 (de) 2000-07-11 2002-01-24 Bayer Ag Superparamagnetische Perlpolymerisate
EP1306446A4 (en) * 2000-08-02 2004-10-20 Oriental Yeast Co Ltd TEST SETS FOR THE EXTRACTION OF NUCLEIC ACIDS AND METHODS FOR THE EXTRACTION OF NUCLEIC ACIDS BY USING THE TEST SETS
JP2003000248A (ja) * 2001-04-25 2003-01-07 Tosoh Corp 改良された核酸抽出方法
DE60230788D1 (de) 2001-11-06 2009-02-26 Cortex Biochem Inc Isolierung und aufreinigung von nukleinsäuren
US8426126B2 (en) * 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
AT501380B1 (de) * 2005-02-07 2007-11-15 Vitali Dipl Ing Dshemelinski Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat
JP2008092835A (ja) * 2006-10-10 2008-04-24 Ebara Jitsugyo Co Ltd 核酸抽出方法
JP5428272B2 (ja) 2008-10-06 2014-02-26 東ソー株式会社 サバイビンｍＲＮＡの測定方法
JP5743666B2 (ja) * 2011-04-15 2015-07-01 英二 小西 Ｒｎａ検出用試薬およびｒｎａ検出方法
JP5743701B2 (ja) * 2011-05-10 2015-07-01 英二 小西 Ｒｎａ検出用試薬およびｒｎａ検出方法
JP5743704B2 (ja) * 2011-05-12 2015-07-01 住友大阪セメント株式会社 Ｒｎａ検出用試薬およびｒｎａ検出方法
US10837011B2 (en) 2014-03-26 2020-11-17 Universal Bio Research Co., Ltd. Method and reagent for extracting nucleic acid

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
EP0389063A2 (en) * 1989-03-23 1990-09-26 Akzo Nobel N.V. Process for isolating nucleic acid
EP0393744A1 (en) * 1989-04-17 1990-10-24 Johnson & Johnson Clinical Diagnostics, Inc. Methods of extracting, amplifying and detecting a nucleic acid from PBMC blood fraction
EP0487218A1 (en) * 1990-10-31 1992-05-27 Tosoh Corporation Method for detecting or quantifying target nucleic acid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
EP0389063A2 (en) * 1989-03-23 1990-09-26 Akzo Nobel N.V. Process for isolating nucleic acid
EP0393744A1 (en) * 1989-04-17 1990-10-24 Johnson & Johnson Clinical Diagnostics, Inc. Methods of extracting, amplifying and detecting a nucleic acid from PBMC blood fraction
EP0487218A1 (en) * 1990-10-31 1992-05-27 Tosoh Corporation Method for detecting or quantifying target nucleic acid

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8519119B2 (en) 2009-08-28 2013-08-27 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US11739382B2 (en) 2019-08-27 2023-08-29 Roche Molecular Systems, Inc. Compositions and methods for amplification and detection of hepatitis B virus RNA, including HBV RNA transcribed from cccDNA

Also Published As

Publication number Publication date
DE4333805A1 (de) 1995-03-02
JPH0759572A (ja) 1995-03-07
JP3451667B2 (ja) 2003-09-29

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Legal Events

Date Code Title Description
8110 Request for examination paragraph 44
8304 Grant after examination procedure
8364 No opposition during term of opposition
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee

Effective date: 20110502