DE3839948A1 - Elektrophoresevorrichtung zur durchfuehrung der horizontalen oder vertikalen gelelektrophorese - Google Patents
Elektrophoresevorrichtung zur durchfuehrung der horizontalen oder vertikalen gelelektrophoreseInfo
- Publication number
- DE3839948A1 DE3839948A1 DE19883839948 DE3839948A DE3839948A1 DE 3839948 A1 DE3839948 A1 DE 3839948A1 DE 19883839948 DE19883839948 DE 19883839948 DE 3839948 A DE3839948 A DE 3839948A DE 3839948 A1 DE3839948 A1 DE 3839948A1
- Authority
- DE
- Germany
- Prior art keywords
- plate
- buffer solution
- electrophoresis
- electrophoresis device
- hinge
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 44
- 238000001502 gel electrophoresis Methods 0.000 title claims abstract description 12
- 239000007853 buffer solution Substances 0.000 claims abstract description 55
- 238000000926 separation method Methods 0.000 claims abstract description 46
- 239000000499 gel Substances 0.000 claims abstract description 12
- 229920002521 macromolecule Polymers 0.000 claims abstract description 6
- 102000004506 Blood Proteins Human genes 0.000 claims abstract description 4
- 108010017384 Blood Proteins Proteins 0.000 claims abstract description 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- 229920002401 polyacrylamide Polymers 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 9
- 229910000831 Steel Inorganic materials 0.000 claims description 8
- 239000010959 steel Substances 0.000 claims description 8
- 229920002472 Starch Polymers 0.000 claims description 3
- 239000011543 agarose gel Substances 0.000 claims description 3
- 238000012512 characterization method Methods 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 238000001155 isoelectric focusing Methods 0.000 abstract description 6
- 238000000539 two dimensional gel electrophoresis Methods 0.000 abstract description 4
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 5
- 239000000872 buffer Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000019271 petrolatum Nutrition 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000819038 Chichester Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- -1 hydroxyl ions Chemical class 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-O oxonium Chemical compound [OH3+] XLYOFNOQVPJJNP-UHFFFAOYSA-O 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical class [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Electrostatic Separation (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19883839948 DE3839948A1 (de) | 1988-11-26 | 1988-11-26 | Elektrophoresevorrichtung zur durchfuehrung der horizontalen oder vertikalen gelelektrophorese |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19883839948 DE3839948A1 (de) | 1988-11-26 | 1988-11-26 | Elektrophoresevorrichtung zur durchfuehrung der horizontalen oder vertikalen gelelektrophorese |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3839948A1 true DE3839948A1 (de) | 1989-05-03 |
| DE3839948C2 DE3839948C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1989-08-03 |
Family
ID=6367940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19883839948 Granted DE3839948A1 (de) | 1988-11-26 | 1988-11-26 | Elektrophoresevorrichtung zur durchfuehrung der horizontalen oder vertikalen gelelektrophorese |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3839948A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5717134A (en) * | 1995-02-23 | 1998-02-10 | Robert Bosch Gmbh | Process and device for testing the function of an electronically controlling brake system |
| US5882495A (en) * | 1996-11-12 | 1999-03-16 | Proteome, Inc. | Electrophoresis system |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2141245A1 (de) * | 1971-08-17 | 1973-03-01 | Max Planck Gesellschaft | Apparatur zur durchfuehrung der traegerfreien kontinuierlichen elektrophorese und/oder magnetophorese sowie ein verfahren zur verwendung dieser apparatur |
| US3766047A (en) * | 1972-09-11 | 1973-10-16 | F Elevitch | Gel for electrophoresis |
| US3773647A (en) * | 1972-05-12 | 1973-11-20 | Electro Nucleonics | Low voltage electrophoretic testing system |
| US3932265A (en) * | 1974-03-28 | 1976-01-13 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis apparatus |
| US3980540A (en) * | 1975-03-28 | 1976-09-14 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis apparatus and method |
| DE2013844B2 (de) * | 1970-03-23 | 1978-02-23 | Verfahren und vorrichtung zur isoelektrischen fokussierung unter stabilisierung des ph-gradienten mit einem gel | |
| DE3032070A1 (de) * | 1980-08-26 | 1982-07-01 | Nikolaus Dr. 6900 Heidelberg Grubhofer | Verfahren zur herstellung von duennen schichten von waessrigem polyacrylamid-gel auf kunststoff-folien fuer die elektrophorese |
| DE2915415C2 (de) * | 1979-04-17 | 1983-06-23 | Stiftung Deutsches Krebsforschungszentrum, 6900 Heidelberg | Elektrophoresegerät |
| US4481094A (en) * | 1982-05-17 | 1984-11-06 | Techamerica Group, Inc. | Stabilized polyacrylamide gels and system for SDS electrophoresis |
-
1988
- 1988-11-26 DE DE19883839948 patent/DE3839948A1/de active Granted
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2013844B2 (de) * | 1970-03-23 | 1978-02-23 | Verfahren und vorrichtung zur isoelektrischen fokussierung unter stabilisierung des ph-gradienten mit einem gel | |
| DE2141245A1 (de) * | 1971-08-17 | 1973-03-01 | Max Planck Gesellschaft | Apparatur zur durchfuehrung der traegerfreien kontinuierlichen elektrophorese und/oder magnetophorese sowie ein verfahren zur verwendung dieser apparatur |
| US3773647A (en) * | 1972-05-12 | 1973-11-20 | Electro Nucleonics | Low voltage electrophoretic testing system |
| US3766047A (en) * | 1972-09-11 | 1973-10-16 | F Elevitch | Gel for electrophoresis |
| US3932265A (en) * | 1974-03-28 | 1976-01-13 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis apparatus |
| US3980540A (en) * | 1975-03-28 | 1976-09-14 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis apparatus and method |
| DE2915415C2 (de) * | 1979-04-17 | 1983-06-23 | Stiftung Deutsches Krebsforschungszentrum, 6900 Heidelberg | Elektrophoresegerät |
| DE3032070A1 (de) * | 1980-08-26 | 1982-07-01 | Nikolaus Dr. 6900 Heidelberg Grubhofer | Verfahren zur herstellung von duennen schichten von waessrigem polyacrylamid-gel auf kunststoff-folien fuer die elektrophorese |
| US4481094A (en) * | 1982-05-17 | 1984-11-06 | Techamerica Group, Inc. | Stabilized polyacrylamide gels and system for SDS electrophoresis |
Non-Patent Citations (3)
| Title |
|---|
| DE-Z.: MITTELBACH F., ANTL W., HAMBURGER K.: Z. klin. Chem. u. klin. Biochem. 7, 1969, 189-193 * |
| NL-B.: DEYL Z. (editor), EVERAERTS F.M., PRUSIK Z. and SVENDSEN P.J. (co-editors): Electrophoresis a survey of techniques and applications, part A, techniques, Journal of Chromatography Library, Vol. 18, 1979, Elsevier Scientific Publishing Company Amsterdam - Oxford - New York * |
| US-B.: GAAL Ö., MEDGYESII G.A., VERECZKEY L.: Electrophoresis in the Separation of Biologi- cal Macromolecules, John Wiley and Sons, Chichester, New York, Brisbane, Toronto, 1980 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5717134A (en) * | 1995-02-23 | 1998-02-10 | Robert Bosch Gmbh | Process and device for testing the function of an electronically controlling brake system |
| US5882495A (en) * | 1996-11-12 | 1999-03-16 | Proteome, Inc. | Electrophoresis system |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3839948C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1989-08-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OAV | Applicant agreed to the publication of the unexamined application as to paragraph 31 lit. 2 z1 | ||
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8322 | Nonbinding interest in granting licenses declared | ||
| 8339 | Ceased/non-payment of the annual fee |