DE3587391D1 - Verfahren zur rueckgewinnung von gereinigten wachstumshormonen aus genetisch modifizierten mikro-organismen. - Google Patents
Verfahren zur rueckgewinnung von gereinigten wachstumshormonen aus genetisch modifizierten mikro-organismen.Info
- Publication number
- DE3587391D1 DE3587391D1 DE8585110371T DE3587391T DE3587391D1 DE 3587391 D1 DE3587391 D1 DE 3587391D1 DE 8585110371 T DE8585110371 T DE 8585110371T DE 3587391 T DE3587391 T DE 3587391T DE 3587391 D1 DE3587391 D1 DE 3587391D1
- Authority
- DE
- Germany
- Prior art keywords
- hormone
- analog
- purified
- ultrafiltration
- separating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000122 growth hormone Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 244000005700 microbiome Species 0.000 title 1
- 238000011084 recovery Methods 0.000 title 1
- 239000005556 hormone Substances 0.000 abstract 7
- 229940088597 hormone Drugs 0.000 abstract 7
- 230000001580 bacterial effect Effects 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- 239000002244 precipitate Substances 0.000 abstract 3
- 239000000725 suspension Substances 0.000 abstract 3
- 238000000108 ultra-filtration Methods 0.000 abstract 3
- 102000018997 Growth Hormone Human genes 0.000 abstract 2
- 108010051696 Growth Hormone Proteins 0.000 abstract 2
- 239000006166 lysate Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000287828 Gallus gallus Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 239000002198 insoluble material Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000011236 particulate material Substances 0.000 abstract 1
- 239000012465 retentate Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 230000003381 solubilizing effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 238000000527 sonication Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US64443584A | 1984-08-27 | 1984-08-27 | |
| US73445085A | 1985-05-15 | 1985-05-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3587391D1 true DE3587391D1 (de) | 1993-07-15 |
| DE3587391T2 DE3587391T2 (de) | 1994-01-13 |
Family
ID=27094481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE85110371T Expired - Lifetime DE3587391T2 (de) | 1984-08-27 | 1985-08-19 | Verfahren zur Rückgewinnung von gereinigten Wachstumshormonen aus genetisch modifizierten Mikro-organismen. |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP0173215B1 (de) |
| JP (1) | JPH0655154B2 (de) |
| KR (1) | KR930009336B1 (de) |
| AT (1) | ATE90389T1 (de) |
| AU (1) | AU589569B2 (de) |
| CA (1) | CA1267615A (de) |
| DE (1) | DE3587391T2 (de) |
| DK (1) | DK174889B1 (de) |
| ES (1) | ES8606496A1 (de) |
| GR (1) | GR852031B (de) |
| HK (1) | HK147695A (de) |
| HU (1) | HU201806B (de) |
| IE (1) | IE58860B1 (de) |
| IL (1) | IL76191A (de) |
| NZ (1) | NZ213153A (de) |
| PT (1) | PT81012B (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| CA1272144A (en) * | 1984-06-29 | 1990-07-31 | Tamio Mizukami | Fish growth hormone polypeptide |
| US4748234A (en) * | 1985-06-26 | 1988-05-31 | Cetus Corporation | Process for recovering refractile bodies containing heterologous proteins from microbial hosts |
| US5064943A (en) * | 1988-12-16 | 1991-11-12 | American Cyanamid Company | Method for solubilization and naturation of somatotropin |
| US4975529A (en) * | 1989-08-18 | 1990-12-04 | Monsanto Company | Method of folding somatotropins |
| DK0432419T3 (da) * | 1989-12-05 | 1994-11-14 | American Cyanamid Co | Fremgangsmåde til solubilisering og naturering af somatotropiner under anvendelse af lav urinstofkoncentration |
| KR940005594B1 (ko) * | 1991-12-24 | 1994-06-21 | 주식회사 럭키 | 양 성장 호르몬의 정제방법 |
| KR100229420B1 (ko) * | 1992-05-09 | 1999-11-01 | 성재갑 | 대장균에서 발현된 닭 성장 호르몬의 정제 방법 |
| US5437986A (en) * | 1994-06-22 | 1995-08-01 | Schering Corporation | Extraction of heterologous insoluble proteins from bacteria |
| KR970061918A (ko) * | 1996-02-22 | 1997-09-12 | 성재갑 | 대장균에서 발현된 유전자 재조합 닭 성장 호르몬의 정제방법 |
| US6316240B1 (en) | 1999-01-25 | 2001-11-13 | Novozymes A/S | Recovery of a glycosidase or peptidase from a culture broth at high pH |
| US6566329B1 (en) | 1999-06-28 | 2003-05-20 | Novo Nordisk A/S | Freeze-dried preparation of human growth hormone |
| KR101460266B1 (ko) * | 2012-06-05 | 2014-11-11 | 씨제이헬스케어 주식회사 | 신규한 지속형 인간 성장호르몬의 정제 방법 |
| CN104379598B (zh) | 2012-06-05 | 2017-04-05 | Cj医药健康株式会社 | 高度糖基化的长效人生长激素蛋白及其生产方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0111814A3 (de) * | 1982-12-16 | 1985-08-14 | Mgi Pharma, Inc. | Klonierung und Expression von nucleotiden Sequenzen die für Rinderwachstumshormon kodieren |
| GR79124B (de) * | 1982-12-22 | 1984-10-02 | Genentech Inc | |
| BG49718A3 (en) * | 1983-07-15 | 1992-01-15 | Bio Technology General Corp | Method for preparing of polypeptid with superoxiddismutasne activitty |
| CA1340597C (en) * | 1984-08-27 | 1999-06-22 | Haim Aviv | Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods |
-
1985
- 1985-08-15 CA CA000488765A patent/CA1267615A/en not_active Expired - Lifetime
- 1985-08-16 KR KR1019850005918A patent/KR930009336B1/ko not_active IP Right Cessation
- 1985-08-16 AU AU46268/85A patent/AU589569B2/en not_active Expired
- 1985-08-19 AT AT85110371T patent/ATE90389T1/de not_active IP Right Cessation
- 1985-08-19 DE DE85110371T patent/DE3587391T2/de not_active Expired - Lifetime
- 1985-08-19 EP EP85110371A patent/EP0173215B1/de not_active Expired - Lifetime
- 1985-08-20 NZ NZ213153A patent/NZ213153A/xx unknown
- 1985-08-20 DK DK198503779A patent/DK174889B1/da not_active IP Right Cessation
- 1985-08-22 GR GR852031A patent/GR852031B/el unknown
- 1985-08-22 JP JP60184984A patent/JPH0655154B2/ja not_active Expired - Fee Related
- 1985-08-22 HU HU853203A patent/HU201806B/hu unknown
- 1985-08-23 ES ES546395A patent/ES8606496A1/es not_active Expired
- 1985-08-23 PT PT81012A patent/PT81012B/pt unknown
- 1985-08-26 IE IE209285A patent/IE58860B1/en not_active IP Right Cessation
- 1985-08-26 IL IL76191A patent/IL76191A/xx not_active IP Right Cessation
-
1995
- 1995-09-14 HK HK147695A patent/HK147695A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0173215A3 (en) | 1987-11-25 |
| KR870002258A (ko) | 1987-03-30 |
| EP0173215B1 (de) | 1993-06-09 |
| ES8606496A1 (es) | 1986-04-16 |
| IE58860B1 (en) | 1993-11-17 |
| PT81012B (pt) | 1987-09-18 |
| PT81012A (en) | 1985-09-01 |
| KR930009336B1 (ko) | 1993-09-27 |
| EP0173215A2 (de) | 1986-03-05 |
| GR852031B (de) | 1985-12-30 |
| ES546395A0 (es) | 1986-04-16 |
| AU589569B2 (en) | 1989-10-19 |
| HUT38398A (en) | 1986-05-28 |
| CA1267615A (en) | 1990-04-10 |
| IE852092L (en) | 1986-02-27 |
| IL76191A0 (en) | 1985-12-31 |
| DE3587391T2 (de) | 1994-01-13 |
| IL76191A (en) | 1990-12-23 |
| AU4626885A (en) | 1986-03-06 |
| HU201806B (en) | 1990-12-28 |
| JPS6193128A (ja) | 1986-05-12 |
| NZ213153A (en) | 1988-07-28 |
| JPH0655154B2 (ja) | 1994-07-27 |
| DK174889B1 (da) | 2004-01-19 |
| ATE90389T1 (de) | 1993-06-15 |
| HK147695A (en) | 1995-09-22 |
| DK377985A (da) | 1986-02-28 |
| DK377985D0 (da) | 1985-08-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |