ES8606496A1 - Un metodo para recuperar una hormona de crecimiento eucario-tica purificada - Google Patents

Un metodo para recuperar una hormona de crecimiento eucario-tica purificada

Info

Publication number
ES8606496A1
ES8606496A1 ES546395A ES546395A ES8606496A1 ES 8606496 A1 ES8606496 A1 ES 8606496A1 ES 546395 A ES546395 A ES 546395A ES 546395 A ES546395 A ES 546395A ES 8606496 A1 ES8606496 A1 ES 8606496A1
Authority
ES
Spain
Prior art keywords
hormone
analog
ultrafiltration
separating
purified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546395A
Other languages
English (en)
Other versions
ES546395A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Savient Pharmaceuticals Inc
Original Assignee
Savient Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Savient Pharmaceuticals Inc filed Critical Savient Pharmaceuticals Inc
Publication of ES546395A0 publication Critical patent/ES546395A0/es
Publication of ES8606496A1 publication Critical patent/ES8606496A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)

Abstract

METODO DE RECUPERACION DE HORMONAS DE CRECIMIENTO EUCARIOTICAS PURIFICADAS O SUS ANALOGOS, A PARTIR DE LAS CELULAS BACTERIANAS EN LAS QUE SE HAN PRODUCIDO MEDIANTE LA EXPRESION DE UNA SECUENCIA DE DNA CODIFICANTE DE DICHAS HORMONAS. COMPRENDE ROMPER LA PARED CELULAR DE LA CELULA BACTERIANA O SUS FRAGMENTOS; SEPARAR EL PRECIPITADO QUE CONTIENE MATERIAL PARTICULADO E INSOLUBLE DEL LISADO FORMADO; PREPARAR CON ESTE UNA SUSPENSION EN UNA DISOLUCION REGULADORA; SEPARAR EL MATERIAL QUE HAYA PRECIPITADO Y SOLUBILIZARLO EN SUFICIENTE DISOLUCION ACUOSA, EN LA QUE SE HA AJUSTADO EL PH A UN VALOR ALCALINO. SEPARAR LA HORMONA O ANALOGO SOLUBILIZADOS MEDIANTE ULTRAFILTRACION, TRATARLOS PARA VOLVER A SOLUBILIZARLOS Y RECUPERARLOS. TIENE APLICACIONES PARA RECUPERAR HORMONAS DE CRECIMIENTO BOVINAS, PORCINAS, DE POLLO Y HUMANAS.
ES546395A 1984-08-27 1985-08-23 Un metodo para recuperar una hormona de crecimiento eucario-tica purificada Expired ES8606496A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64443584A 1984-08-27 1984-08-27
US73445085A 1985-05-15 1985-05-15

Publications (2)

Publication Number Publication Date
ES546395A0 ES546395A0 (es) 1986-04-16
ES8606496A1 true ES8606496A1 (es) 1986-04-16

Family

ID=27094481

Family Applications (1)

Application Number Title Priority Date Filing Date
ES546395A Expired ES8606496A1 (es) 1984-08-27 1985-08-23 Un metodo para recuperar una hormona de crecimiento eucario-tica purificada

Country Status (16)

Country Link
EP (1) EP0173215B1 (es)
JP (1) JPH0655154B2 (es)
KR (1) KR930009336B1 (es)
AT (1) ATE90389T1 (es)
AU (1) AU589569B2 (es)
CA (1) CA1267615A (es)
DE (1) DE3587391T2 (es)
DK (1) DK174889B1 (es)
ES (1) ES8606496A1 (es)
GR (1) GR852031B (es)
HK (1) HK147695A (es)
HU (1) HU201806B (es)
IE (1) IE58860B1 (es)
IL (1) IL76191A (es)
NZ (1) NZ213153A (es)
PT (1) PT81012B (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
CA1272144A (en) * 1984-06-29 1990-07-31 Tamio Mizukami Fish growth hormone polypeptide
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
US4975529A (en) * 1989-08-18 1990-12-04 Monsanto Company Method of folding somatotropins
DE69013475T2 (de) * 1989-12-05 1995-05-04 American Cyanamid Co Methode zur Auflösung und Naturation von Somatotropin unter Verwendung von einer niedrigen Harnstoffkonzentration.
KR940005594B1 (ko) * 1991-12-24 1994-06-21 주식회사 럭키 양 성장 호르몬의 정제방법
KR100229420B1 (ko) * 1992-05-09 1999-11-01 성재갑 대장균에서 발현된 닭 성장 호르몬의 정제 방법
US5437986A (en) * 1994-06-22 1995-08-01 Schering Corporation Extraction of heterologous insoluble proteins from bacteria
KR970061918A (ko) * 1996-02-22 1997-09-12 성재갑 대장균에서 발현된 유전자 재조합 닭 성장 호르몬의 정제방법
US6316240B1 (en) 1999-01-25 2001-11-13 Novozymes A/S Recovery of a glycosidase or peptidase from a culture broth at high pH
US6566329B1 (en) 1999-06-28 2003-05-20 Novo Nordisk A/S Freeze-dried preparation of human growth hormone
JP6231559B2 (ja) 2012-06-05 2017-11-15 シージェイ ヘルスケア コーポレイションＣｊ Ｈｅａｌｔｈｃａｒｅ Ｃｏｒｐｏｒａｔｉｏｎ 高グリコシル化された持続型ヒト成長ホルモンタンパク質の製造方法及び精製方法
KR101460266B1 (ko) * 2012-06-05 2014-11-11 씨제이헬스케어 주식회사 신규한 지속형 인간 성장호르몬의 정제 방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0111814A3 (en) * 1982-12-16 1985-08-14 Mgi Pharma, Inc. The cloning and expression of nucleotide sequences encoding bovine growth hormone
GR79124B (es) * 1982-12-22 1984-10-02 Genentech Inc
BG49718A3 (en) * 1983-07-15 1992-01-15 Bio Technology General Corp Method for preparing of polypeptid with superoxiddismutasne activitty
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods

Also Published As

Publication number Publication date
DE3587391D1 (de) 1993-07-15
HU201806B (en) 1990-12-28
PT81012A (en) 1985-09-01
JPH0655154B2 (ja) 1994-07-27
IL76191A0 (en) 1985-12-31
EP0173215B1 (en) 1993-06-09
KR930009336B1 (ko) 1993-09-27
EP0173215A2 (en) 1986-03-05
HK147695A (en) 1995-09-22
AU589569B2 (en) 1989-10-19
CA1267615A (en) 1990-04-10
DE3587391T2 (de) 1994-01-13
JPS6193128A (ja) 1986-05-12
KR870002258A (ko) 1987-03-30
ES546395A0 (es) 1986-04-16
DK377985A (da) 1986-02-28
DK377985D0 (da) 1985-08-20
PT81012B (pt) 1987-09-18
ATE90389T1 (de) 1993-06-15
EP0173215A3 (en) 1987-11-25
NZ213153A (en) 1988-07-28
DK174889B1 (da) 2004-01-19
AU4626885A (en) 1986-03-06
IE58860B1 (en) 1993-11-17
IE852092L (en) 1986-02-27
IL76191A (en) 1990-12-23
HUT38398A (en) 1986-05-28
GR852031B (es) 1985-12-30

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20051219