DE3504748A1 - Verfahren zur kultivierung von zellen in hoher dichte - Google Patents
Verfahren zur kultivierung von zellen in hoher dichteInfo
- Publication number
- DE3504748A1 DE3504748A1 DE19853504748 DE3504748A DE3504748A1 DE 3504748 A1 DE3504748 A1 DE 3504748A1 DE 19853504748 DE19853504748 DE 19853504748 DE 3504748 A DE3504748 A DE 3504748A DE 3504748 A1 DE3504748 A1 DE 3504748A1
- Authority
- DE
- Germany
- Prior art keywords
- cells
- medium
- gas
- oxygen
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 40
- 210000004027 cell Anatomy 0.000 claims description 130
- 239000007789 gas Substances 0.000 claims description 72
- 239000002609 medium Substances 0.000 claims description 68
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 50
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 50
- 229910052760 oxygen Inorganic materials 0.000 claims description 50
- 239000001301 oxygen Substances 0.000 claims description 50
- 239000002775 capsule Substances 0.000 claims description 39
- 239000001569 carbon dioxide Substances 0.000 claims description 25
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 25
- 230000005587 bubbling Effects 0.000 claims description 14
- 210000004102 animal cell Anatomy 0.000 claims description 13
- 210000004962 mammalian cell Anatomy 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 10
- 230000011278 mitosis Effects 0.000 claims description 8
- 210000002421 cell wall Anatomy 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 230000035899 viability Effects 0.000 claims description 5
- 238000012423 maintenance Methods 0.000 claims description 4
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 2
- 230000001133 acceleration Effects 0.000 claims 1
- 230000004060 metabolic process Effects 0.000 claims 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 17
- 238000004113 cell culture Methods 0.000 description 15
- 239000000243 solution Substances 0.000 description 10
- 230000012010 growth Effects 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- 239000006260 foam Substances 0.000 description 5
- 210000004408 hybridoma Anatomy 0.000 description 5
- 239000003094 microcapsule Substances 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 4
- 231100000572 poisoning Toxicity 0.000 description 4
- 230000000607 poisoning effect Effects 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 238000005538 encapsulation Methods 0.000 description 3
- 239000012737 fresh medium Substances 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- MKWKNSIESPFAQN-UHFFFAOYSA-N N-cyclohexyl-2-aminoethanesulfonic acid Chemical compound OS(=O)(=O)CCNC1CCCCC1 MKWKNSIESPFAQN-UHFFFAOYSA-N 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000000661 sodium alginate Substances 0.000 description 2
- 235000010413 sodium alginate Nutrition 0.000 description 2
- 229940005550 sodium alginate Drugs 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000008000 CHES buffer Substances 0.000 description 1
- 241001315286 Damon Species 0.000 description 1
- 206010018746 Growth accelerated Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000000648 calcium alginate Substances 0.000 description 1
- 235000010410 calcium alginate Nutrition 0.000 description 1
- 229960002681 calcium alginate Drugs 0.000 description 1
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000004098 cellular respiration Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- ZFTFAPZRGNKQPU-UHFFFAOYSA-N dicarbonic acid Chemical compound OC(=O)OC(O)=O ZFTFAPZRGNKQPU-UHFFFAOYSA-N 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- -1 iron (III) ions Chemical class 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57949384A | 1984-02-13 | 1984-02-13 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3504748A1 true DE3504748A1 (de) | 1985-09-05 |
| DE3504748C2 DE3504748C2 (enrdf_load_stackoverflow) | 1987-07-23 |
Family
ID=24317121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19853504748 Granted DE3504748A1 (de) | 1984-02-13 | 1985-02-12 | Verfahren zur kultivierung von zellen in hoher dichte |
Country Status (11)
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3504715A1 (de) * | 1984-02-13 | 1985-09-05 | Damon Biotech, Inc., Needham Heights, Mass. | Verfahren und medium zur foerderung der proteinproduktion von saeugerzellen |
| EP1673452B1 (en) | 2003-10-10 | 2015-12-23 | Novo Nordisk Health Care AG | Method for large-scale production of a polypeptide in eukaryote cells |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4829002A (en) * | 1986-05-12 | 1989-05-09 | Baxter International Inc. | System for metering nutrient media to cell culture containers and method |
| WO1988000967A1 (en) * | 1986-08-04 | 1988-02-11 | The University Of New South Wales | Serum free tissue culture medium containing polymeric cell-protective agent |
| JPS6423888A (en) * | 1987-07-16 | 1989-01-26 | Etsuko Kakizaki | Culture vessel with micro-cellular wall |
| JP2509630B2 (ja) * | 1987-07-29 | 1996-06-26 | 三井石油化学工業株式会社 | 培養方法及び培養装置 |
| DE3739650C1 (de) * | 1987-11-23 | 1989-05-24 | Immuno Ag | Fermenter zum Zuechten von Zellkulturen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4352883A (en) * | 1979-03-28 | 1982-10-05 | Damon Corporation | Encapsulation of biological material |
| US4409331A (en) * | 1979-03-28 | 1983-10-11 | Damon Corporation | Preparation of substances with encapsulated cells |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4724206A (en) * | 1984-02-13 | 1988-02-09 | Damon Biotech, Inc. | Protein production using hypertonic media |
-
1985
- 1985-02-07 NL NL8500351A patent/NL8500351A/nl not_active Application Discontinuation
- 1985-02-08 GB GB08503248A patent/GB2154246B/en not_active Expired
- 1985-02-08 AU AU38571/85A patent/AU572608B2/en not_active Expired - Fee Related
- 1985-02-11 BE BE0/214491A patent/BE901702A/fr not_active IP Right Cessation
- 1985-02-12 FR FR8501963A patent/FR2559500A1/fr active Pending
- 1985-02-12 DK DK65085A patent/DK65085A/da not_active Application Discontinuation
- 1985-02-12 IT IT67141/85A patent/IT1184884B/it active
- 1985-02-12 DE DE19853504748 patent/DE3504748A1/de active Granted
- 1985-02-12 SE SE8500622A patent/SE8500622L/xx not_active Application Discontinuation
- 1985-02-12 NO NO850534A patent/NO850534L/no unknown
- 1985-02-13 JP JP60026134A patent/JPS60199380A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4352883A (en) * | 1979-03-28 | 1982-10-05 | Damon Corporation | Encapsulation of biological material |
| US4409331A (en) * | 1979-03-28 | 1983-10-11 | Damon Corporation | Preparation of substances with encapsulated cells |
Non-Patent Citations (2)
| Title |
|---|
| BERNHAUER: Gärungschemisches Praktikum, 1939, 78 * |
| Engineering Principles and Scule-up Trends in Biotechnology, 1983, 102 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3504715A1 (de) * | 1984-02-13 | 1985-09-05 | Damon Biotech, Inc., Needham Heights, Mass. | Verfahren und medium zur foerderung der proteinproduktion von saeugerzellen |
| EP1673452B1 (en) | 2003-10-10 | 2015-12-23 | Novo Nordisk Health Care AG | Method for large-scale production of a polypeptide in eukaryote cells |
Also Published As
| Publication number | Publication date |
|---|---|
| IT8567141A0 (it) | 1985-02-12 |
| BE901702A (fr) | 1985-05-29 |
| IT1184884B (it) | 1987-10-28 |
| GB8503248D0 (en) | 1985-03-13 |
| SE8500622L (sv) | 1985-08-14 |
| JPS6110115B2 (enrdf_load_stackoverflow) | 1986-03-28 |
| JPS60199380A (ja) | 1985-10-08 |
| NL8500351A (nl) | 1985-09-02 |
| GB2154246A (en) | 1985-09-04 |
| SE8500622D0 (sv) | 1985-02-12 |
| AU3857185A (en) | 1985-08-22 |
| GB2154246B (en) | 1987-09-09 |
| FR2559500A1 (fr) | 1985-08-16 |
| NO850534L (no) | 1985-08-14 |
| DK65085A (da) | 1985-08-14 |
| AU572608B2 (en) | 1988-05-12 |
| DK65085D0 (da) | 1985-02-12 |
| DE3504748C2 (enrdf_load_stackoverflow) | 1987-07-23 |
| IT8567141A1 (it) | 1986-08-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8363 | Opposition against the patent | ||
| 8330 | Complete renunciation |