JPS6423888A - Culture vessel with micro-cellular wall - Google Patents

Abstract

PURPOSE:To obtain a culture vessel with a micro-cellular wall surface, having a pore diameter capable of aeration at the minimum and passing through a solution at the maximum and the maximum pore diameter without passing cells therethrough. CONSTITUTION:A culture vessel obtained by using a porous polymer, such as Porous(R) manufactured by Teijin Ltd. or Entrant(R) manufactured by Toray Industries, Inc., micro-cellular synthetic polymer or glass prepared so as to provide the pore diameter of size with air permeability at the minimum without passing a solution or cells therethrough at the maximum and forming a wall having a thickness larger than that capable of withstanding at least the weight of contents of cells or nutriculture solution into an optional shape, such as bottle or bag. The above-mentioned vessel is sterilized by gas treatment, overheating treatment, etc., to afford the aimed vessel. The nutriculture solution and cells are placed in the afore- mentioned vessel and cultivated to smoothly vent generated gases and reduce foaming in the vessel. The above-mentioned vessel is placed in a water tank and gaseous carbon dioxide is fed and taken in the vessel. The vessel prepared by adjusting so that the pore diameter may pass a solution at the maximum without passing cells therethrough is placed in the water tank and growth components, etc., are added to allow the vessel to stand. Gaseous carbon dioxide is fed to take in the growth components and gaseous carbon dioxide in the vessel.