CN216585033U

CN216585033U - Incubator device for cell culture

Info

Publication number: CN216585033U
Application number: CN202123218135.XU
Authority: CN
Inventors: 毕晓玉; 杨少斌; 汝昆; 蔺亚妮
Original assignee: Tianjin Jiankang Huamei Medical Diagnosis Technology Co ltd
Current assignee: Tianjin Jiankang Huamei Medical Diagnosis Technology Co ltd
Priority date: 2021-12-20
Filing date: 2021-12-20
Publication date: 2022-05-24
Anticipated expiration: 2031-12-20

Abstract

The utility model relates to an incubator device for cell culture, which comprises a culture bracket, a fixed cover and a plurality of groups of culture tubes, wherein the culture tubes can be horizontally arranged so as to be inserted on a bracket body and subjected to sample adding operation, and can also be obliquely arranged so as to incline the culture tubes, so that the liquid level area of a culture medium is effectively increased, meanwhile, the device can also realize repeated reverse and uniform shaking operation of the culture tubes, so that cultures in the culture tubes can be fully mixed, the device is provided with the fixed cover, the culture tubes can be arranged in a relatively closed cavity structure through the fixed cover and the culture bracket, and CO2 gas and air are introduced into the cavity through connecting holes and ventilation holes, so that the growth of the culture medium is more facilitated.

Description

Incubator device for cell culture

Technical Field

The utility model belongs to the technical field of medical instruments, and relates to an incubator device for cell culture.

Background

The incubator used in cell culture is commonly referred to as CO₂An incubator with a gas atmosphere requiring 5% CO₂. Therefore, the culture medium must have air holes for CO to pass through₂The gas can enter the culture medium to maintain the pH value of the culture solution, so that the cells can grow better. After the original culture medium is laid flat, the culture solution can block the air holes to influence CO₂Of the mobile terminal. And the culture medium that has inoculated needs to reverse the mixing, but at present because the fixed knot of culture tube adopts porous structure's mount more, when the culture tube needs to reverse the mixing, the mount can't closely fix the culture tube position, consequently needs a can realize that the culture tube reverses repeatedly and shakes the even, can keep the mount structure that certain angle slope was placed again simultaneously.

Chinese patent document CN207002699U discloses a culture tube rack capable of being placed obliquely. It can only the level be placed for overcoming current test-tube rack, leads to the test tube can only vertically stand, be unfavorable for carrying out the defect of cell culture at the in-vitro, includes in this patent technical scheme the lateral part of support body is equipped with puts support piece to one side, put support piece to one side and include the holding surface, the holding surface holds the axis deflection of chamber and constitutes contained angle alpha for the test tube, wherein alpha < 90, the support body can rotate to holding surface and mesa laminating along with putting support piece to one side to the support body that makes the slope obtains the support. The advantages are that: the test-tube rack can wholly incline and place, and the contained angle that support body and mesa constitute this moment equals with alpha, and the test tube can be for mesa or horizontal plane slope, avoids the culture to block up the bleeder vent, influences CO 2's entering, and then influences cell growth, but the device can't realize simultaneously that a plurality of culture tubes reverse to shake evenly, and this structure still has certain defect.

SUMMERY OF THE UTILITY MODEL

Aiming at the problems in the prior art, the utility model discloses an incubator device for cell culture, which can be horizontally placed so that a culture tube is inserted on a frame body and sample adding operation is carried out, can also be obliquely placed so that the culture tube is inclined, the liquid level area of a culture medium is effectively increased, and meanwhile, the device can also realize repeated reverse and uniform shaking operation of a plurality of culture tubes, so that cultures in the culture tubes can be fully mixed.

The technical scheme adopted by the utility model for solving the technical problems is as follows: an incubator device for cell culture comprises a culture bracket, a fixing cover and a plurality of groups of culture tubes, wherein the culture bracket adopts a multi-layer plate fixing structure and comprises a bottom support, two side vertical plates and two layers of fixing plates, the bottom support is horizontally arranged at the lowest layer of the culture bracket, the two side vertical plates are relatively fixed on the side surface of the bottom support, the two layers of fixing plates are kept parallel to the bottom support, and the side surfaces of the fixing plates are fixedly connected with the side vertical plates; a plurality of fixing holes are uniformly formed in the surfaces of the two layers of fixing plates, and the positions of the fixing holes of the upper fixing plate and the lower fixing plate correspond to each other; the culture tubes are respectively fixed in the fixing holes, the fixing cover is positioned above the culture tubes, and the side surface of the fixing cover is fixedly connected with the culture bracket; and supporting mechanisms for supporting the side lying culture bracket are respectively arranged on the side vertical plates of the culture bracket.

Furthermore, the supporting mechanism adopts a supporting plate in a triangular structure, one side edge of the supporting plate is fixedly connected with the edge, close to the top, of the side vertical plate, and the width of the supporting plate is gradually increased from bottom to top in the vertical state of the culture support.

Furthermore, the supporting mechanism comprises a wedge-shaped supporting plate, an adjusting nut and a connecting screw, wherein the wedge-shaped supporting plate is located at one edge of the surface of the side vertical plate, the wedge-shaped supporting plate is connected with the side vertical plate through the connecting screw, the adjusting nut is in threaded connection with the connecting screw, and the wedge-shaped supporting plate is pressed on the side vertical plate through screwing the adjusting nut.

Furthermore, the surface of each side vertical plate in the culture support is respectively provided with at least one connecting buckle, the side surface of the fixed cover is provided with a connecting bulge at the position corresponding to the connecting buckle, and the fixed cover is fixed on the culture support through the matching of the connecting buckles and the connecting bulges.

Furthermore, the connection hasp comprises a hasp fixing part, a hasp bending part and a hasp matching part, the hasp fixing part is fixed on the surface of the side vertical plate of the culture support, the hasp fixing part is connected with the hasp matching part through the hasp bending part, and the hasp matching part is matched with the connection bulge.

Furthermore, the top of the culture tube is provided with a vent hole for communicating the culture medium in the tube body with the outside air.

Furthermore, the diameter of a fixing hole on the uppermost fixing plate in the culture bracket is larger than that of a fixing hole on the lower fixing plate.

Furthermore, the side surface of the fixed cover is provided with a connecting hole for introducing CO2 gas.

Furthermore, a plurality of air holes are uniformly distributed on the surface of the top of the fixing cover.

Compared with the prior art, the utility model has the following advantages:

1) the culture box device can be horizontally placed so that the culture tubes can be inserted on the frame body and sample adding operation can be conveniently carried out, the culture tubes can also be obliquely placed so that the culture tubes can be inclined, the liquid level area of a culture medium can be effectively increased, meanwhile, the device can also realize repeated reverse shaking operation of a plurality of culture tubes, cultures in the culture tubes can be fully mixed, the device is provided with a fixed cover, the culture tubes can be arranged in a relatively closed cavity structure through the fixed cover and the culture support, and CO is introduced into the cavity through the connecting holes and the air vents₂Gas and air are more beneficial to the growth of the culture medium.

Drawings

FIG. 1 is a schematic view showing the construction of an incubator apparatus according to the present embodiment;

FIG. 2 is an exploded view of an incubator assembly according to the present embodiment;

FIG. 3 is a sectional view showing the internal structure of an incubator apparatus according to the present embodiment;

FIG. 4 is an enlarged schematic view at A in FIG. 3;

FIG. 5 is a schematic side view of a support mechanism on the culture support in this embodiment;

FIG. 6 is a schematic view showing the structure of an adjustable support mechanism of the culture support of the present embodiment;

FIG. 7 is a schematic side view of an adjustable support mechanism of the culture support of this embodiment.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Example (b):

as shown in fig. 1-3, the incubator apparatus for cell culture in this embodiment comprises a culture support 1, a fixing cover 3 and a plurality of groups of culture tubes 2, wherein the culture support 1 adopts a multi-layer plate fixing structure, the culture support 1 comprises a base, two side vertical plates and two layers of fixing plates, the base is horizontally arranged at the lowest layer of the culture support 1, the two side vertical plates are fixed to the side surfaces of the base in an opposite manner, the two layers of fixing plates are parallel to the base, and the side surfaces of the fixing plates are fixedly connected with the side vertical plates; the surfaces of the two layers of fixing plates are uniformly provided with a plurality of fixing holes, the positions of the fixing holes of the upper and lower fixing plates correspond to each other, and the diameter of the fixing hole on the uppermost layer of fixing plate in the culture bracket 1 is larger than that of the fixing hole on the lower layer of fixing plate; the culture tubes 2 are respectively fixed in the fixing holes, the fixing cover 3 is positioned above the culture tubes 2, and the side surface of the fixing cover 3 is fixedly connected with the culture support 1; and the side vertical plates of the culture bracket 1 are respectively provided with a supporting mechanism 4 for supporting the culture bracket 1 in a side lying state. Specifically, as shown in fig. 5, the supporting mechanism 4 may be a supporting plate having a triangular structure, one side of the supporting plate is fixedly connected to the edge of the side vertical plate near the top, and the width of the supporting plate is gradually increased from bottom to top in the vertical state of the culture support 1. When the culture support 1 is in the state of lying on one side, the side surface of the culture support 1 and the horizontal plane form an included angle, the culture tube 2 is also laid on one side along with the included angle, so that the liquid surface area of the internal culture medium is increased, the total height of the liquid is reduced, cells can be distributed on the culture base part in a large range, the situation that nutrient substances on the upper part of the culture medium cannot be fully utilized is avoided, and the contact area between the cells and gas is increased.

However, the above-mentioned supporting mechanism 4 adopts a fixed structure, the inclined angle of the culture rack 1 is fixed when lying on the side, and in order to further make the inclined angle of the culture rack 1 adjustable, so as to adjust the inclined degree of the liquid level in the culture tube 2, alternatively, the supporting mechanism includes a wedge-shaped supporting plate 401, an adjusting nut 402 and a connecting screw 403, as shown in fig. 6-7, wherein the wedge-shaped supporting plate 401 is located at an edge position of the surface of the side standing plate, the wedge-shaped supporting plate 401 is connected with the side standing plate by the connecting screw 403, and the adjusting nut 402 is in threaded connection with the connecting screw 403, and the wedge-shaped supporting plate 401 is pressed on the side standing plate by screwing the adjusting nut 402. By adjusting the rotation angle of the wedge-shaped supporting plate 401, the culture bracket 1 can be adjusted when being inclined, so that the device is suitable for culture tubes 2 with different liquid level heights, and has better universality.

In addition, as shown in fig. 3 and fig. 4, at least one connection buckle 5 is respectively arranged on the surface of each side plate in the culture support 1, a connection protrusion 301 is arranged on the side surface of the fixing cover 3 at a position corresponding to the connection buckle 5, and the fixing cover 3 is fixed on the culture support 1 by the cooperation of the connection buckle 5 and the connection protrusion 301. More specifically, the connection buckle 5 comprises a buckle fixing part 501, a buckle bending part 502 and a buckle matching part 503, wherein the buckle fixing part 501 is fixed on the surface of the side vertical plate of the culture support 1, the buckle fixing part 501 and the buckle matching part 503 are connected through the buckle bending part 502, and the buckle matching part 503 is matched with the connection bulge 301. The fixing cover 3 can be fixed to the culture support 1 by the coupling snap 5 and the coupling projection 301, and when the culture tube 2 is turned upside down, it is ensured that the culture tube 2 does not come off the culture support 1.

And as a further improvement, the top of the culture tube 2 is provided with a vent hole for communicating the culture medium in the tube body with the outside air. The side surface of the fixed cover 3 is provided with a connecting hole for introducing CO2 gas. And a plurality of air holes are uniformly distributed on the surface of the top of the fixed cover 3. Since the gaseous environment during cell culture requires 5% CO 2. Therefore, the culture tube 2 must have a vent hole to enable CO2 gas to enter the culture tube 2 to maintain the pH value of the culture solution, so that cells can grow better, and the concentration of CO2 in the gas can be manually adjusted through the connecting hole and the vent hole on the fixed cover 3 to ensure that the cells are always in a 5% CO2 environment in the culture process, thereby promoting cell culture.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims

1. The incubator device for cell culture is characterized by comprising a culture bracket, a fixing cover and a plurality of groups of culture tubes, wherein the culture bracket adopts a multi-layer plate fixing structure and comprises a bottom support, two side vertical plates and two layers of fixing plates, the bottom support is horizontally arranged at the lowest layer of the culture bracket, the two side vertical plates are relatively fixed on the side surface of the bottom support, the two layers of fixing plates are parallel to the bottom support, and the side surfaces of the fixing plates are fixedly connected with the side vertical plates; a plurality of fixing holes are uniformly formed in the surfaces of the two layers of fixing plates, and the positions of the fixing holes of the upper fixing plate and the lower fixing plate correspond to each other; the culture tubes are respectively fixed in the fixing holes, the fixing cover is positioned above the culture tubes, and the side surface of the fixing cover is fixedly connected with the culture bracket; and supporting mechanisms for supporting the side lying culture bracket are respectively arranged on the side vertical plates of the culture bracket.

2. The incubator apparatus according to claim 1, wherein the support mechanism is a support plate having a triangular structure, one side of the support plate is fixedly connected to the edge of the side vertical plate near the top, and the width of the support plate is gradually increased from bottom to top in a vertical state of the cultivation support.

3. The incubator device according to claim 1, wherein the support mechanism comprises a wedge-shaped support plate, an adjusting nut and a connecting screw, wherein the wedge-shaped support plate is located at an edge of the surface of the side standing plate, the wedge-shaped support plate and the side standing plate are connected by the connecting screw, the adjusting nut is screwed on the connecting screw, and the wedge-shaped support plate is pressed on the side standing plate by screwing the adjusting nut.

4. The incubator apparatus according to claim 1, wherein the surface of each side plate of the cultivation support is provided with at least one connection buckle, the side of the fixing cover is provided with a connection protrusion at a position corresponding to the connection buckle, and the fixing cover is fixed on the cultivation support by the cooperation of the connection buckle and the connection protrusion.

5. The incubator apparatus according to claim 4, wherein the connection buckle comprises a buckle fixing portion fixed to a surface of the side vertical plate of the culture support, a buckle bending portion connected to the buckle fixing portion via the buckle bending portion, and a buckle fitting portion fitted to the connection protrusion.

6. The incubator assembly according to claim 1 wherein the top of the culture tube is provided with a vent for communicating the medium inside the tube with the outside atmosphere.

7. The incubator apparatus according to claim 1, wherein the diameter of the fixing hole of the uppermost fixing plate in the culture support is larger than the diameter of the fixing hole of the lower fixing plate.

8. Incubator assembly according to claim 1, characterised in that the side of the fixed cover is provided with means for introducing CO₂And a gas connecting hole.

9. The incubator assembly according to claim 1, wherein the top surface of the fixing cover is uniformly distributed with a plurality of ventilation holes.