DE2754252A1 - Trennungszone fuer chromatographie, verfahren zur chromatographischen trennung und dafuer zu verwendende pulver - Google Patents
Trennungszone fuer chromatographie, verfahren zur chromatographischen trennung und dafuer zu verwendende pulverInfo
- Publication number
- DE2754252A1 DE2754252A1 DE19772754252 DE2754252A DE2754252A1 DE 2754252 A1 DE2754252 A1 DE 2754252A1 DE 19772754252 DE19772754252 DE 19772754252 DE 2754252 A DE2754252 A DE 2754252A DE 2754252 A1 DE2754252 A1 DE 2754252A1
- Authority
- DE
- Germany
- Prior art keywords
- macroparticles
- pore size
- bimodal
- distribution
- separation zone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000926 separation method Methods 0.000 title claims description 57
- 238000000034 method Methods 0.000 title claims description 25
- 238000013375 chromatographic separation Methods 0.000 title claims description 9
- 238000004587 chromatography analysis Methods 0.000 title description 9
- 239000011148 porous material Substances 0.000 claims description 183
- 238000009826 distribution Methods 0.000 claims description 105
- 230000002902 bimodal effect Effects 0.000 claims description 76
- 238000011088 calibration curve Methods 0.000 claims description 51
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 47
- 239000011859 microparticle Substances 0.000 claims description 32
- 239000000463 material Substances 0.000 claims description 29
- 239000000843 powder Substances 0.000 claims description 19
- 239000000377 silicon dioxide Substances 0.000 claims description 17
- 230000014759 maintenance of location Effects 0.000 claims description 16
- 235000012239 silicon dioxide Nutrition 0.000 claims description 8
- 239000002356 single layer Substances 0.000 claims description 2
- 210000002231 macronucleus Anatomy 0.000 claims 3
- 229910052710 silicon Inorganic materials 0.000 claims 1
- 239000010703 silicon Substances 0.000 claims 1
- 239000002245 particle Substances 0.000 description 55
- 239000000945 filler Substances 0.000 description 8
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 6
- 229910052753 mercury Inorganic materials 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 5
- 238000001542 size-exclusion chromatography Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 4
- 239000000908 ammonium hydroxide Substances 0.000 description 4
- 230000007717 exclusion Effects 0.000 description 4
- 238000011049 filling Methods 0.000 description 4
- 239000010410 layer Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000002216 antistatic agent Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000011049 pearl Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 238000010908 decantation Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 238000002459 porosimetry Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910021485 fumed silica Inorganic materials 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000010954 inorganic particle Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000006082 mold release agent Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000006069 physical mixture Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229910021426 porous silicon Inorganic materials 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
- B01J20/103—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate comprising silica
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28004—Sorbent size or size distribution, e.g. particle size
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28016—Particle form
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
- B01J20/28088—Pore-size distribution
- B01J20/28092—Bimodal, polymodal, different types of pores or different pore size distributions in different parts of the sorbent
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
- B01J20/283—Porous sorbents based on silica
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3291—Characterised by the shape of the carrier, the coating or the obtained coated product
- B01J20/3295—Coatings made of particles, nanoparticles, fibers, nanofibers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/52—Physical parameters
- G01N2030/524—Physical parameters structural properties
- G01N2030/525—Physical parameters structural properties surface properties, e.g. porosity
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74876976A | 1976-12-08 | 1976-12-08 | |
| US05/826,171 US4160728A (en) | 1976-12-08 | 1977-08-22 | Bimodal chromatographic resolving zone |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2754252A1 true DE2754252A1 (de) | 1978-06-15 |
| DE2754252C2 DE2754252C2 (enExample) | 1989-03-09 |
Family
ID=27114997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19772754252 Granted DE2754252A1 (de) | 1976-12-08 | 1977-12-06 | Trennungszone fuer chromatographie, verfahren zur chromatographischen trennung und dafuer zu verwendende pulver |
Country Status (12)
| Country | Link |
|---|---|
| JP (1) | JPS5371899A (enExample) |
| CA (1) | CA1101827A (enExample) |
| CH (1) | CH634661A5 (enExample) |
| DE (1) | DE2754252A1 (enExample) |
| DK (1) | DK544977A (enExample) |
| FR (1) | FR2373316B1 (enExample) |
| GB (1) | GB1592885A (enExample) |
| IE (1) | IE45932B1 (enExample) |
| IT (1) | IT1089344B (enExample) |
| LU (1) | LU78658A1 (enExample) |
| NL (1) | NL7713517A (enExample) |
| SE (1) | SE442591B (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2506166B (en) * | 2012-09-24 | 2014-12-17 | Thermo Electron Mfg Ltd | Improvements in and relating to chromatography columns |
| GB2506165B (en) * | 2012-09-24 | 2017-04-12 | Thermo Electron Mfg Ltd | Improvements in and relating to chromatography columns |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3505785A (en) * | 1967-06-20 | 1970-04-14 | Du Pont | Superficially porous supports for chromatography |
-
1977
- 1977-12-06 CA CA292,467A patent/CA1101827A/en not_active Expired
- 1977-12-06 DE DE19772754252 patent/DE2754252A1/de active Granted
- 1977-12-07 GB GB5102277A patent/GB1592885A/en not_active Expired
- 1977-12-07 IT IT3052077A patent/IT1089344B/it active
- 1977-12-07 IE IE248377A patent/IE45932B1/en unknown
- 1977-12-07 SE SE7713892A patent/SE442591B/sv not_active IP Right Cessation
- 1977-12-07 FR FR7736829A patent/FR2373316B1/fr not_active Expired
- 1977-12-07 NL NL7713517A patent/NL7713517A/xx not_active Application Discontinuation
- 1977-12-07 DK DK544977A patent/DK544977A/da not_active Application Discontinuation
- 1977-12-08 LU LU78658A patent/LU78658A1/xx unknown
- 1977-12-08 CH CH1508077A patent/CH634661A5/de not_active IP Right Cessation
- 1977-12-08 JP JP14770777A patent/JPS5371899A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3505785A (en) * | 1967-06-20 | 1970-04-14 | Du Pont | Superficially porous supports for chromatography |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2373316A1 (fr) | 1978-07-07 |
| JPS6410779B2 (enExample) | 1989-02-22 |
| SE7713892L (sv) | 1978-06-09 |
| IE45932L (en) | 1978-06-08 |
| NL7713517A (nl) | 1978-06-12 |
| SE442591B (sv) | 1986-01-20 |
| IE45932B1 (en) | 1982-12-29 |
| DE2754252C2 (enExample) | 1989-03-09 |
| JPS5371899A (en) | 1978-06-26 |
| DK544977A (da) | 1978-06-09 |
| LU78658A1 (enExample) | 1978-07-11 |
| IT1089344B (it) | 1985-06-18 |
| CA1101827A (en) | 1981-05-26 |
| CH634661A5 (en) | 1983-02-15 |
| GB1592885A (en) | 1981-07-08 |
| FR2373316B1 (fr) | 1985-07-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8125 | Change of the main classification |
Ipc: B01J 20/28 |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition |