DE2737513B2 - Verfahren zur Bestimmung von Phospholipiden und Reagens zur Durchführung des Verfahrens - Google Patents

Verfahren zur Bestimmung von Phospholipiden und Reagens zur Durchführung des Verfahrens

Info

Publication number
DE2737513B2
DE2737513B2 DE19772737513 DE2737513A DE2737513B2 DE 2737513 B2 DE2737513 B2 DE 2737513B2 DE 19772737513 DE19772737513 DE 19772737513 DE 2737513 A DE2737513 A DE 2737513A DE 2737513 B2 DE2737513 B2 DE 2737513B2
Authority
DE
Germany
Prior art keywords
choline
lecithin
reaction
phospholipase
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
DE19772737513
Other languages
German (de)
English (en)
Other versions
DE2737513A1 (de
Inventor
Hiroaki Wako Saitama Hayashi
Toshio Numazu Shizuoka Tatano
Katsuyuki Tokio Watanabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Publication of DE2737513A1 publication Critical patent/DE2737513A1/de
Publication of DE2737513B2 publication Critical patent/DE2737513B2/de
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/92—Nitro blue tetrazolium chloride, i.e. NBT
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/906—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE19772737513 1976-08-19 1977-08-19 Verfahren zur Bestimmung von Phospholipiden und Reagens zur Durchführung des Verfahrens Ceased DE2737513B2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9823376A JPS5324888A (en) 1976-08-19 1976-08-19 Composition for quantitating fats containing phosphorus

Publications (2)

Publication Number Publication Date
DE2737513A1 DE2737513A1 (de) 1978-02-23
DE2737513B2 true DE2737513B2 (de) 1980-02-14

Family

ID=14214230

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19772737513 Ceased DE2737513B2 (de) 1976-08-19 1977-08-19 Verfahren zur Bestimmung von Phospholipiden und Reagens zur Durchführung des Verfahrens

Country Status (4)

Country Link
JP (1) JPS5324888A (enExample)
CA (1) CA1081589A (enExample)
DE (1) DE2737513B2 (enExample)
FR (1) FR2362394A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4409987C1 (de) * 1994-03-23 1995-07-06 Wolfgang Dr Bernhard Hochdruck-Flüssigkeitschromatographieverfahren zur Trennung der Hauptphospholipidklassen

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5937737B2 (ja) * 1978-05-25 1984-09-11 新日本製鐵株式会社 熱間鍛造用鋼材
IT1157281B (it) * 1981-06-25 1987-02-11 Toyo Jozo Kk Metodo di saggio per un componente relativo ai lipidi, composizione per il saggio e procedimento per la produzione dell'enzima utilizzato allo scopo
DE3128480A1 (de) * 1981-07-18 1983-01-27 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur bestimmung von lecithin in fruchtwasser sowie reagens zur durchfuehrung dieses verfahrens
JPS58107199A (ja) * 1981-12-16 1983-06-25 Toyo Jozo Co Ltd ホスフアチジルグリセロ−ルの定量方法
JP4527925B2 (ja) * 2000-02-08 2010-08-18 シスメックス株式会社 腎障害の検査方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4409987C1 (de) * 1994-03-23 1995-07-06 Wolfgang Dr Bernhard Hochdruck-Flüssigkeitschromatographieverfahren zur Trennung der Hauptphospholipidklassen

Also Published As

Publication number Publication date
FR2362394A1 (fr) 1978-03-17
DE2737513A1 (de) 1978-02-23
JPS5324888A (en) 1978-03-08
FR2362394B1 (enExample) 1981-08-28
CA1081589A (en) 1980-07-15

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Legal Events

Date Code Title Description
OD Request for examination
8235 Patent refused