CA1081589A - Composition and method for the quantitative determination of phospholipids - Google Patents

Composition and method for the quantitative determination of phospholipids

Info

Publication number
CA1081589A
CA1081589A CA284,986A CA284986A CA1081589A CA 1081589 A CA1081589 A CA 1081589A CA 284986 A CA284986 A CA 284986A CA 1081589 A CA1081589 A CA 1081589A
Authority
CA
Canada
Prior art keywords
choline
reaction
phospholipase
lecithin
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA284,986A
Other languages
English (en)
French (fr)
Inventor
Hiroaki Hayashi
Katsuyuki Watanabe
Toshio Tatano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Application granted granted Critical
Publication of CA1081589A publication Critical patent/CA1081589A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/92—Nitro blue tetrazolium chloride, i.e. NBT
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/906—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA284,986A 1976-08-19 1977-08-18 Composition and method for the quantitative determination of phospholipids Expired CA1081589A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP98233/76 1976-08-19
JP9823376A JPS5324888A (en) 1976-08-19 1976-08-19 Composition for quantitating fats containing phosphorus

Publications (1)

Publication Number Publication Date
CA1081589A true CA1081589A (en) 1980-07-15

Family

ID=14214230

Family Applications (1)

Application Number Title Priority Date Filing Date
CA284,986A Expired CA1081589A (en) 1976-08-19 1977-08-18 Composition and method for the quantitative determination of phospholipids

Country Status (4)

Country Link
JP (1) JPS5324888A (enExample)
CA (1) CA1081589A (enExample)
DE (1) DE2737513B2 (enExample)
FR (1) FR2362394A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5937737B2 (ja) * 1978-05-25 1984-09-11 新日本製鐵株式会社 熱間鍛造用鋼材
IT1157281B (it) * 1981-06-25 1987-02-11 Toyo Jozo Kk Metodo di saggio per un componente relativo ai lipidi, composizione per il saggio e procedimento per la produzione dell'enzima utilizzato allo scopo
DE3128480A1 (de) * 1981-07-18 1983-01-27 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur bestimmung von lecithin in fruchtwasser sowie reagens zur durchfuehrung dieses verfahrens
JPS58107199A (ja) * 1981-12-16 1983-06-25 Toyo Jozo Co Ltd ホスフアチジルグリセロ−ルの定量方法
DE4409987C1 (de) * 1994-03-23 1995-07-06 Wolfgang Dr Bernhard Hochdruck-Flüssigkeitschromatographieverfahren zur Trennung der Hauptphospholipidklassen
JP4527925B2 (ja) * 2000-02-08 2010-08-18 シスメックス株式会社 腎障害の検査方法

Also Published As

Publication number Publication date
FR2362394A1 (fr) 1978-03-17
DE2737513A1 (de) 1978-02-23
DE2737513B2 (de) 1980-02-14
JPS5324888A (en) 1978-03-08
FR2362394B1 (enExample) 1981-08-28

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Legal Events

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