DE2455870A1 - Verfahren und vorrichtung zur klassifizierung biologischer zellen durch fluoreszente strahlung - Google Patents
Verfahren und vorrichtung zur klassifizierung biologischer zellen durch fluoreszente strahlungInfo
- Publication number
- DE2455870A1 DE2455870A1 DE19742455870 DE2455870A DE2455870A1 DE 2455870 A1 DE2455870 A1 DE 2455870A1 DE 19742455870 DE19742455870 DE 19742455870 DE 2455870 A DE2455870 A DE 2455870A DE 2455870 A1 DE2455870 A1 DE 2455870A1
- Authority
- DE
- Germany
- Prior art keywords
- light
- cells
- cell
- classification
- wavelengths
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 13
- 230000005855 radiation Effects 0.000 title claims description 9
- 230000004044 response Effects 0.000 claims description 14
- 230000005856 abnormality Effects 0.000 claims description 7
- 230000001427 coherent effect Effects 0.000 claims description 5
- 238000005259 measurement Methods 0.000 claims description 2
- 230000004043 responsiveness Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 85
- 108020004414 DNA Proteins 0.000 description 5
- 230000002159 abnormal effect Effects 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 3
- JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 description 3
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000003211 malignant effect Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- NOFPXGWBWIPSHI-UHFFFAOYSA-N 2,7,9-trimethylacridine-3,6-diamine;hydrochloride Chemical compound Cl.CC1=C(N)C=C2N=C(C=C(C(C)=C3)N)C3=C(C)C2=C1 NOFPXGWBWIPSHI-UHFFFAOYSA-N 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 208000001860 Eye Infections Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010043866 Tinea capitis Diseases 0.000 description 1
- KZENBFUSKMWCJF-UHFFFAOYSA-N [5-[5-[5-(hydroxymethyl)-2-thiophenyl]-2-furanyl]-2-thiophenyl]methanol Chemical compound S1C(CO)=CC=C1C1=CC=C(C=2SC(CO)=CC=2)O1 KZENBFUSKMWCJF-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009118 appropriate response Effects 0.000 description 1
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- -1 argon ion Chemical class 0.000 description 1
- KSCQDDRPFHTIRL-UHFFFAOYSA-N auramine O Chemical compound [H+].[Cl-].C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 KSCQDDRPFHTIRL-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 208000011323 eye infectious disease Diseases 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012543 microbiological analysis Methods 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41968973A | 1973-11-28 | 1973-11-28 | |
| US516090A US3916197A (en) | 1973-11-28 | 1974-10-18 | Method and apparatus for classifying biological cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2455870A1 true DE2455870A1 (de) | 1975-06-19 |
Family
ID=27024586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19742455870 Pending DE2455870A1 (de) | 1973-11-28 | 1974-11-26 | Verfahren und vorrichtung zur klassifizierung biologischer zellen durch fluoreszente strahlung |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US3916197A (enExample) |
| JP (1) | JPS5087395A (enExample) |
| CA (1) | CA1019975A (enExample) |
| DE (1) | DE2455870A1 (enExample) |
| GB (1) | GB1471969A (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3304795A1 (de) * | 1982-03-09 | 1983-09-22 | Lawrence 44233 Hinckley Ohio Kass | Verfahren zur differentiellen bestimmung der entwicklungsstufen neutrophiler, granulozytischer zellen und anderer leukozyten mittels metachromatischer frabstoffadsorption und einer fluoreszenzlicht-emissionseinrichtung |
| DE3219620A1 (de) * | 1982-05-25 | 1983-12-01 | Vsesojuznyj naučno-issledovatel'skij institut biosinteza belkovych veščestv, Moskva | Verfahren zur ermittlung des verhaeltnisses von lebenden und toten zellen in hefesuspensionen |
| EP2236596A1 (en) * | 2009-04-03 | 2010-10-06 | Synoptics Limited | High fidelity colour imaging of microbial colonies |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2437984B2 (de) * | 1974-08-07 | 1976-06-10 | Wilhelm Will Kg, Optisches Werk, 6331 Nauborn | Verfahren zur kontrastverbesserung eines optischen mikroskopes |
| US4031398A (en) * | 1976-03-23 | 1977-06-21 | Research Corporation | Video fluorometer |
| DE2732272C2 (de) * | 1977-07-16 | 1979-07-05 | Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts, 6900 Heidelberg | Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefärbten Partikeln, insbesondere biologischen Zellen |
| SE7806922L (sv) * | 1978-06-15 | 1979-12-16 | Svenska Traeforskningsinst | Forfarande och anordning for att indikera storleksfordelningen av i ett strommande medium befintliga partiklar |
| US4172227A (en) * | 1978-07-21 | 1979-10-23 | Becton, Dickinson And Company | Flow microfluorometer |
| US4242447A (en) * | 1978-11-29 | 1980-12-30 | Bioresearch | Rapid detection of bacteria |
| US4354114A (en) * | 1979-10-09 | 1982-10-12 | Karnaukhov Valery N | Apparatus for investigation of fluorescence characteristics of microscopic objects |
| GB2097144A (en) * | 1981-03-23 | 1982-10-27 | Tno | New method for identifying algae in water samples and apparatus for use in that method |
| JPS58118948A (ja) * | 1982-01-06 | 1983-07-15 | Takeshi Nishisaka | 分光分析による異常細胞の検出方法及び装置 |
| US4499052A (en) * | 1982-08-30 | 1985-02-12 | Becton, Dickinson And Company | Apparatus for distinguishing multiple subpopulations of cells |
| US4717655A (en) * | 1982-08-30 | 1988-01-05 | Becton, Dickinson And Company | Method and apparatus for distinguishing multiple subpopulations of cells |
| JPS59184862A (ja) * | 1983-04-05 | 1984-10-20 | ベクトン・ディッキンソン・アンド・カンパニ− | 試料内細胞の複数副次集団を識別する方法と装置 |
| JPS60181637A (ja) * | 1984-02-28 | 1985-09-17 | Mitsubishi Electric Corp | メタン菌の菌数またはメタン生成活性の測定方法 |
| US4686372A (en) * | 1983-05-09 | 1987-08-11 | Mitsubishi Denki Kabushiki Kaisha | Method and apparatus for measuring cell counts of Methanogens or methane producing activity thereof |
| JPS59205998A (ja) * | 1983-05-09 | 1984-11-21 | Mitsubishi Electric Corp | メタン菌の菌数またはメタン生成活性の測定方法 |
| US4609286A (en) * | 1984-04-16 | 1986-09-02 | Becton, Dickinson And Company | Dispersion prism for separation of wavelengths of spectrally rich light in a flow cytometry apparatus |
| JPS61186854A (ja) * | 1985-02-14 | 1986-08-20 | Fuji Photo Film Co Ltd | 超純水中のバクテリア数測定装置 |
| US4727020A (en) * | 1985-02-25 | 1988-02-23 | Becton, Dickinson And Company | Method for analysis of subpopulations of blood cells |
| US5175109A (en) * | 1986-09-10 | 1992-12-29 | Toa Medical Electronics Co., Ltd. | Reagent for classifying leukocytes by flow cytometry |
| US5179026A (en) * | 1986-11-27 | 1993-01-12 | Toa Medical Electronics Co., Ltd. | Method of classifying leukocytes by flow cytometry and reagents used in the method |
| US5039613A (en) * | 1986-11-27 | 1991-08-13 | Toa Medical Electronics Co., Ltd. | Reagents used in a method of classifying leukocytes by flow cytometry |
| JPH0782009B2 (ja) * | 1988-03-24 | 1995-09-06 | 工業技術院長 | 細胞融合方法及び装置 |
| DE3825352A1 (de) * | 1988-07-26 | 1990-02-01 | Kessler Manfred | Verfahren und vorrichtung zur bestimmung von lokalen farbstoff-konzentrationen und von streuparametern in tierischen und menschlichen geweben |
| US5760900A (en) * | 1989-03-18 | 1998-06-02 | Canon Kabushiki Kaisha | Method and apparatus for optically measuring specimen |
| JP3213333B2 (ja) * | 1991-05-14 | 2001-10-02 | シスメックス株式会社 | 尿中の細胞分析装置および方法。 |
| JP3213334B2 (ja) * | 1991-05-14 | 2001-10-02 | シスメックス株式会社 | 尿中の細胞分析装置 |
| US5999256A (en) * | 1992-02-12 | 1999-12-07 | Cambridge Consultants Limited | Particle measurement system |
| CA2092373A1 (en) * | 1992-04-24 | 1993-10-25 | Klaus W. Berndt | Methods and apparatus for detecting biological activities in a specimen |
| US5432061A (en) * | 1992-09-01 | 1995-07-11 | Becton Dickinson And Company | Method and apparatus for detecting microorganisms |
| US5710628A (en) * | 1994-12-12 | 1998-01-20 | Visible Genetics Inc. | Automated electrophoresis and fluorescence detection apparatus and method |
| US6014213A (en) * | 1994-12-12 | 2000-01-11 | Visible Genetics Inc. | High dynamic range apparatus for separation and detection of polynucleotide fragments |
| US5793485A (en) * | 1995-03-20 | 1998-08-11 | Sandia Corporation | Resonant-cavity apparatus for cytometry or particle analysis |
| US5608519A (en) * | 1995-03-20 | 1997-03-04 | Gourley; Paul L. | Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells |
| US5733739A (en) * | 1995-06-07 | 1998-03-31 | Inphocyte, Inc. | System and method for diagnosis of disease by infrared analysis of human tissues and cells |
| DE102007047093B4 (de) * | 2007-10-01 | 2010-07-01 | Ferton Holding S.A. | Vorrichtung zur Messung von Fluoreszenzstrahlung an biologischen Substanzen mit einer Halbleitersensorenanordnung |
| EP2194368B1 (de) * | 2008-12-03 | 2019-07-17 | Grundfos Management A/S | Sensorsystem zum Erfassen und Spezifizieren von einzelnen Partikeln in einem Fluid |
| WO2010068670A1 (en) | 2008-12-09 | 2010-06-17 | Advanced Analytical Technologies, Inc. | Capillary electrophoresis fluorescent detection system |
| ES2767133T3 (es) * | 2011-06-24 | 2020-06-16 | Becton Dickinson Co | Citometría de flujo de exploración de espectro de absorción |
| CN104807792B (zh) * | 2015-04-27 | 2017-06-30 | 电子科技大学 | 一种负折射率光子晶体型生物荧光探测器 |
| US10024780B2 (en) | 2015-12-18 | 2018-07-17 | Abbott Laboratories (Diagnostics Division) | Methods for detecting events in a flow cytometer |
| CN114002196B (zh) | 2016-02-22 | 2025-03-28 | 株式会社日立高新技术 | 发光检测装置 |
| DE112016007594B3 (de) * | 2016-02-22 | 2021-06-10 | Hitachi High-Tech Corporation | Lichtemittierende detektionsvorrichtung |
| US10436697B2 (en) * | 2016-11-19 | 2019-10-08 | Cytek Biosciences, Inc. | Flow cytometery system with fluidics control system |
| WO2020241652A1 (ja) * | 2019-05-30 | 2020-12-03 | ソニー株式会社 | 光学測定装置及び光学測定システム |
| WO2021167777A1 (en) * | 2020-02-19 | 2021-08-26 | Becton, Dickinson And Company | Strobed laser excitation systems and methods of use thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3062963A (en) * | 1960-09-29 | 1962-11-06 | Amchem Prod | Method of monitoring colored fluids |
| US3413464A (en) * | 1965-04-29 | 1968-11-26 | Ibm | Method for measuring the nucleic acid in biological cells after enhancement in an acidic solution |
| US3470373A (en) * | 1966-10-18 | 1969-09-30 | Litton Systems Inc | Method for analysis and identification of biologic entities by phosphorescence |
| US3497690A (en) * | 1967-09-21 | 1970-02-24 | Bausch & Lomb | Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof |
-
1974
- 1974-10-18 US US516090A patent/US3916197A/en not_active Expired - Lifetime
- 1974-11-14 CA CA213,712A patent/CA1019975A/en not_active Expired
- 1974-11-26 DE DE19742455870 patent/DE2455870A1/de active Pending
- 1974-11-28 GB GB5159474A patent/GB1471969A/en not_active Expired
- 1974-11-28 JP JP49135978A patent/JPS5087395A/ja active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3304795A1 (de) * | 1982-03-09 | 1983-09-22 | Lawrence 44233 Hinckley Ohio Kass | Verfahren zur differentiellen bestimmung der entwicklungsstufen neutrophiler, granulozytischer zellen und anderer leukozyten mittels metachromatischer frabstoffadsorption und einer fluoreszenzlicht-emissionseinrichtung |
| DE3219620A1 (de) * | 1982-05-25 | 1983-12-01 | Vsesojuznyj naučno-issledovatel'skij institut biosinteza belkovych veščestv, Moskva | Verfahren zur ermittlung des verhaeltnisses von lebenden und toten zellen in hefesuspensionen |
| EP2236596A1 (en) * | 2009-04-03 | 2010-10-06 | Synoptics Limited | High fidelity colour imaging of microbial colonies |
| WO2010112583A1 (en) * | 2009-04-03 | 2010-10-07 | Synoptics Limited | High fidelity colour imaging of microbial colonies |
Also Published As
| Publication number | Publication date |
|---|---|
| GB1471969A (en) | 1977-04-27 |
| CA1019975A (en) | 1977-11-01 |
| JPS5087395A (enExample) | 1975-07-14 |
| US3916197A (en) | 1975-10-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |