CA1019975A - Classifying biological cells by fluorescent radiation using split laser beam - Google Patents

Classifying biological cells by fluorescent radiation using split laser beam

Info

Publication number
CA1019975A
CA1019975A CA213,712A CA213712A CA1019975A CA 1019975 A CA1019975 A CA 1019975A CA 213712 A CA213712 A CA 213712A CA 1019975 A CA1019975 A CA 1019975A
Authority
CA
Canada
Prior art keywords
laser beam
biological cells
fluorescent radiation
split laser
classifying biological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA213,712A
Other languages
English (en)
Other versions
CA213712S (en
Inventor
Mack J. Fulwyler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
Original Assignee
Coulter Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc
Application granted granted Critical
Publication of CA1019975A publication Critical patent/CA1019975A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA213,712A 1973-11-28 1974-11-14 Classifying biological cells by fluorescent radiation using split laser beam Expired CA1019975A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41968973A 1973-11-28 1973-11-28
US516090A US3916197A (en) 1973-11-28 1974-10-18 Method and apparatus for classifying biological cells

Publications (1)

Publication Number Publication Date
CA1019975A true CA1019975A (en) 1977-11-01

Family

ID=27024586

Family Applications (1)

Application Number Title Priority Date Filing Date
CA213,712A Expired CA1019975A (en) 1973-11-28 1974-11-14 Classifying biological cells by fluorescent radiation using split laser beam

Country Status (5)

Country Link
US (1) US3916197A (enExample)
JP (1) JPS5087395A (enExample)
CA (1) CA1019975A (enExample)
DE (1) DE2455870A1 (enExample)
GB (1) GB1471969A (enExample)

Cited By (1)

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US5733739A (en) * 1995-06-07 1998-03-31 Inphocyte, Inc. System and method for diagnosis of disease by infrared analysis of human tissues and cells

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DE2437984B2 (de) * 1974-08-07 1976-06-10 Wilhelm Will Kg, Optisches Werk, 6331 Nauborn Verfahren zur kontrastverbesserung eines optischen mikroskopes
US4031398A (en) * 1976-03-23 1977-06-21 Research Corporation Video fluorometer
DE2732272C2 (de) * 1977-07-16 1979-07-05 Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts, 6900 Heidelberg Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefärbten Partikeln, insbesondere biologischen Zellen
SE7806922L (sv) * 1978-06-15 1979-12-16 Svenska Traeforskningsinst Forfarande och anordning for att indikera storleksfordelningen av i ett strommande medium befintliga partiklar
US4172227A (en) * 1978-07-21 1979-10-23 Becton, Dickinson And Company Flow microfluorometer
US4242447A (en) * 1978-11-29 1980-12-30 Bioresearch Rapid detection of bacteria
US4354114A (en) * 1979-10-09 1982-10-12 Karnaukhov Valery N Apparatus for investigation of fluorescence characteristics of microscopic objects
US4500509A (en) * 1981-03-11 1985-02-19 Lawrence Kass Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
GB2097144A (en) * 1981-03-23 1982-10-27 Tno New method for identifying algae in water samples and apparatus for use in that method
JPS58118948A (ja) * 1982-01-06 1983-07-15 Takeshi Nishisaka 分光分析による異常細胞の検出方法及び装置
DE3219620A1 (de) * 1982-05-25 1983-12-01 Vsesojuznyj naučno-issledovatel'skij institut biosinteza belkovych veščestv, Moskva Verfahren zur ermittlung des verhaeltnisses von lebenden und toten zellen in hefesuspensionen
US4499052A (en) * 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
US4717655A (en) * 1982-08-30 1988-01-05 Becton, Dickinson And Company Method and apparatus for distinguishing multiple subpopulations of cells
JPS59184862A (ja) * 1983-04-05 1984-10-20 ベクトン・ディッキンソン・アンド・カンパニ− 試料内細胞の複数副次集団を識別する方法と装置
JPS60181637A (ja) * 1984-02-28 1985-09-17 Mitsubishi Electric Corp メタン菌の菌数またはメタン生成活性の測定方法
US4686372A (en) * 1983-05-09 1987-08-11 Mitsubishi Denki Kabushiki Kaisha Method and apparatus for measuring cell counts of Methanogens or methane producing activity thereof
JPS59205998A (ja) * 1983-05-09 1984-11-21 Mitsubishi Electric Corp メタン菌の菌数またはメタン生成活性の測定方法
US4609286A (en) * 1984-04-16 1986-09-02 Becton, Dickinson And Company Dispersion prism for separation of wavelengths of spectrally rich light in a flow cytometry apparatus
JPS61186854A (ja) * 1985-02-14 1986-08-20 Fuji Photo Film Co Ltd 超純水中のバクテリア数測定装置
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
JPH0782009B2 (ja) * 1988-03-24 1995-09-06 工業技術院長 細胞融合方法及び装置
DE3825352A1 (de) * 1988-07-26 1990-02-01 Kessler Manfred Verfahren und vorrichtung zur bestimmung von lokalen farbstoff-konzentrationen und von streuparametern in tierischen und menschlichen geweben
US5760900A (en) * 1989-03-18 1998-06-02 Canon Kabushiki Kaisha Method and apparatus for optically measuring specimen
JP3213333B2 (ja) * 1991-05-14 2001-10-02 シスメックス株式会社 尿中の細胞分析装置および方法。
JP3213334B2 (ja) * 1991-05-14 2001-10-02 シスメックス株式会社 尿中の細胞分析装置
US5999256A (en) * 1992-02-12 1999-12-07 Cambridge Consultants Limited Particle measurement system
CA2092373A1 (en) * 1992-04-24 1993-10-25 Klaus W. Berndt Methods and apparatus for detecting biological activities in a specimen
US5432061A (en) * 1992-09-01 1995-07-11 Becton Dickinson And Company Method and apparatus for detecting microorganisms
US5710628A (en) * 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
US6014213A (en) * 1994-12-12 2000-01-11 Visible Genetics Inc. High dynamic range apparatus for separation and detection of polynucleotide fragments
US5793485A (en) * 1995-03-20 1998-08-11 Sandia Corporation Resonant-cavity apparatus for cytometry or particle analysis
US5608519A (en) * 1995-03-20 1997-03-04 Gourley; Paul L. Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells
DE102007047093B4 (de) * 2007-10-01 2010-07-01 Ferton Holding S.A. Vorrichtung zur Messung von Fluoreszenzstrahlung an biologischen Substanzen mit einer Halbleitersensorenanordnung
EP2194368B1 (de) * 2008-12-03 2019-07-17 Grundfos Management A/S Sensorsystem zum Erfassen und Spezifizieren von einzelnen Partikeln in einem Fluid
WO2010068670A1 (en) 2008-12-09 2010-06-17 Advanced Analytical Technologies, Inc. Capillary electrophoresis fluorescent detection system
EP2236596B1 (en) * 2009-04-03 2012-07-25 Synoptics Limited High fidelity colour imaging of microbial colonies
ES2767133T3 (es) * 2011-06-24 2020-06-16 Becton Dickinson Co Citometría de flujo de exploración de espectro de absorción
CN104807792B (zh) * 2015-04-27 2017-06-30 电子科技大学 一种负折射率光子晶体型生物荧光探测器
US10024780B2 (en) 2015-12-18 2018-07-17 Abbott Laboratories (Diagnostics Division) Methods for detecting events in a flow cytometer
CN114002196B (zh) 2016-02-22 2025-03-28 株式会社日立高新技术 发光检测装置
DE112016007594B3 (de) * 2016-02-22 2021-06-10 Hitachi High-Tech Corporation Lichtemittierende detektionsvorrichtung
US10436697B2 (en) * 2016-11-19 2019-10-08 Cytek Biosciences, Inc. Flow cytometery system with fluidics control system
WO2020241652A1 (ja) * 2019-05-30 2020-12-03 ソニー株式会社 光学測定装置及び光学測定システム
WO2021167777A1 (en) * 2020-02-19 2021-08-26 Becton, Dickinson And Company Strobed laser excitation systems and methods of use thereof

Family Cites Families (4)

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US3062963A (en) * 1960-09-29 1962-11-06 Amchem Prod Method of monitoring colored fluids
US3413464A (en) * 1965-04-29 1968-11-26 Ibm Method for measuring the nucleic acid in biological cells after enhancement in an acidic solution
US3470373A (en) * 1966-10-18 1969-09-30 Litton Systems Inc Method for analysis and identification of biologic entities by phosphorescence
US3497690A (en) * 1967-09-21 1970-02-24 Bausch & Lomb Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733739A (en) * 1995-06-07 1998-03-31 Inphocyte, Inc. System and method for diagnosis of disease by infrared analysis of human tissues and cells

Also Published As

Publication number Publication date
DE2455870A1 (de) 1975-06-19
GB1471969A (en) 1977-04-27
JPS5087395A (enExample) 1975-07-14
US3916197A (en) 1975-10-28

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