DE2447781A1 - Verfahren zur herstellung von interferon - Google Patents
Verfahren zur herstellung von interferonInfo
- Publication number
- DE2447781A1 DE2447781A1 DE19742447781 DE2447781A DE2447781A1 DE 2447781 A1 DE2447781 A1 DE 2447781A1 DE 19742447781 DE19742447781 DE 19742447781 DE 2447781 A DE2447781 A DE 2447781A DE 2447781 A1 DE2447781 A1 DE 2447781A1
- Authority
- DE
- Germany
- Prior art keywords
- interferon
- human
- centrifugation
- gradient
- zonal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102000014150 Interferons Human genes 0.000 title claims description 56
- 108010050904 Interferons Proteins 0.000 title claims description 56
- 229940079322 interferon Drugs 0.000 title claims description 54
- 238000004519 manufacturing process Methods 0.000 title description 4
- 238000000034 method Methods 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 16
- 241000282693 Cercopithecidae Species 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 238000000432 density-gradient centrifugation Methods 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 claims description 4
- 241000282461 Canis lupus Species 0.000 claims 1
- 210000002950 fibroblast Anatomy 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 description 9
- 241000700605 Viruses Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000000746 purification Methods 0.000 description 7
- 238000011084 recovery Methods 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 238000002523 gelfiltration Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 239000003443 antiviral agent Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000003189 isokinetic effect Effects 0.000 description 2
- 210000003292 kidney cell Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 101100189378 Caenorhabditis elegans pat-3 gene Proteins 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000711975 Vesicular stomatitis virus Species 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013098 chemical test method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 238000002298 density-gradient ultracentrifugation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 108010025899 gelatin film Proteins 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4686973A GB1475883A (en) | 1973-10-08 | 1973-10-08 | Purification of interferon by means of density gradient centri fugation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2447781A1 true DE2447781A1 (de) | 1975-04-10 |
Family
ID=10442902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19742447781 Withdrawn DE2447781A1 (de) | 1973-10-08 | 1974-10-07 | Verfahren zur herstellung von interferon |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPS5076224A (enrdf_load_stackoverflow) |
| DE (1) | DE2447781A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2246566B1 (enrdf_load_stackoverflow) |
| GB (1) | GB1475883A (enrdf_load_stackoverflow) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2821466A1 (de) * | 1978-05-17 | 1979-11-29 | Thomae Gmbh Dr K | Verbessertes verfahren zur herstellung von humaninterferon |
-
1973
- 1973-10-08 GB GB4686973A patent/GB1475883A/en not_active Expired
-
1974
- 1974-10-07 FR FR7433693A patent/FR2246566B1/fr not_active Expired
- 1974-10-07 DE DE19742447781 patent/DE2447781A1/de not_active Withdrawn
- 1974-10-07 JP JP49115429A patent/JPS5076224A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5076224A (enrdf_load_stackoverflow) | 1975-06-21 |
| FR2246566A1 (enrdf_load_stackoverflow) | 1975-05-02 |
| GB1475883A (en) | 1977-06-10 |
| FR2246566B1 (enrdf_load_stackoverflow) | 1979-06-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8139 | Disposal/non-payment of the annual fee |