DE2306072C2 - Verfahren zur Gewinnung oder Reinigung von Orgotein durch enzymatische Behandlung von Protein-Gemischen - Google Patents
Verfahren zur Gewinnung oder Reinigung von Orgotein durch enzymatische Behandlung von Protein-GemischenInfo
- Publication number
- DE2306072C2 DE2306072C2 DE2306072A DE2306072A DE2306072C2 DE 2306072 C2 DE2306072 C2 DE 2306072C2 DE 2306072 A DE2306072 A DE 2306072A DE 2306072 A DE2306072 A DE 2306072A DE 2306072 C2 DE2306072 C2 DE 2306072C2
- Authority
- DE
- Germany
- Prior art keywords
- orgotein
- proteins
- enzyme
- liver
- pancreatin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 74
- 108090000623 proteins and genes Proteins 0.000 title claims description 74
- 108010070915 orgotein Proteins 0.000 title claims description 67
- 229960004534 orgotein Drugs 0.000 title claims description 67
- TUGDLVFMIQZYPA-UHFFFAOYSA-N tetracopper;tetrazinc Chemical compound [Cu+2].[Cu+2].[Cu+2].[Cu+2].[Zn+2].[Zn+2].[Zn+2].[Zn+2] TUGDLVFMIQZYPA-UHFFFAOYSA-N 0.000 title claims description 67
- 238000000034 method Methods 0.000 title claims description 35
- 239000000203 mixture Substances 0.000 title claims description 34
- 230000002255 enzymatic effect Effects 0.000 title claims description 8
- 238000000746 purification Methods 0.000 title claims description 6
- 238000004519 manufacturing process Methods 0.000 title 1
- 108010019160 Pancreatin Proteins 0.000 claims description 24
- 229940055695 pancreatin Drugs 0.000 claims description 24
- 102000035195 Peptidases Human genes 0.000 claims description 23
- 108091005804 Peptidases Proteins 0.000 claims description 23
- 102000004190 Enzymes Human genes 0.000 claims description 21
- 108090000790 Enzymes Proteins 0.000 claims description 21
- 229940088598 enzyme Drugs 0.000 claims description 21
- 210000004185 liver Anatomy 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 5
- 230000002797 proteolythic effect Effects 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 description 19
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 16
- 108010059712 Pronase Proteins 0.000 description 15
- 239000006228 supernatant Substances 0.000 description 13
- 239000012535 impurity Substances 0.000 description 12
- 239000004365 Protease Substances 0.000 description 11
- 235000019833 protease Nutrition 0.000 description 11
- 235000021336 beef liver Nutrition 0.000 description 10
- 239000000872 buffer Substances 0.000 description 8
- 239000000284 extract Substances 0.000 description 8
- 230000029087 digestion Effects 0.000 description 7
- 239000002002 slurry Substances 0.000 description 7
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- 239000000706 filtrate Substances 0.000 description 6
- 108010004032 Bromelains Proteins 0.000 description 5
- 235000019835 bromelain Nutrition 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 238000004255 ion exchange chromatography Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 4
- 108090000526 Papain Proteins 0.000 description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 108090000787 Subtilisin Proteins 0.000 description 4
- 150000001540 azides Chemical class 0.000 description 4
- 238000000502 dialysis Methods 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 229940040511 liver extract Drugs 0.000 description 4
- 229940055729 papain Drugs 0.000 description 4
- 235000019834 papain Nutrition 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 102000004400 Aminopeptidases Human genes 0.000 description 2
- 108090000915 Aminopeptidases Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000006862 enzymatic digestion Effects 0.000 description 2
- 238000005227 gel permeation chromatography Methods 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 235000020004 porter Nutrition 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- AXAVXPMQTGXXJZ-UHFFFAOYSA-N 2-aminoacetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound NCC(O)=O.OCC(N)(CO)CO AXAVXPMQTGXXJZ-UHFFFAOYSA-N 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 108090000101 Asclepain Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 102000000496 Carboxypeptidases A Human genes 0.000 description 1
- 108010080937 Carboxypeptidases A Proteins 0.000 description 1
- 102000005600 Cathepsins Human genes 0.000 description 1
- 108010084457 Cathepsins Proteins 0.000 description 1
- 108090001081 Dipeptidases Proteins 0.000 description 1
- 102000004860 Dipeptidases Human genes 0.000 description 1
- 108010059378 Endopeptidases Proteins 0.000 description 1
- 102000005593 Endopeptidases Human genes 0.000 description 1
- 108010091443 Exopeptidases Proteins 0.000 description 1
- 102000018389 Exopeptidases Human genes 0.000 description 1
- 108090000270 Ficain Proteins 0.000 description 1
- 108010063312 Metalloproteins Proteins 0.000 description 1
- 102000010750 Metalloproteins Human genes 0.000 description 1
- 101710184866 Mexicain Proteins 0.000 description 1
- -1 NaCl Chemical class 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000223892 Tetrahymena Species 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 102100039662 Xaa-Pro dipeptidase Human genes 0.000 description 1
- SMEGJBVQLJJKKX-HOTMZDKISA-N [(2R,3S,4S,5R,6R)-5-acetyloxy-3,4,6-trihydroxyoxan-2-yl]methyl acetate Chemical compound CC(=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)O)OC(=O)C)O)O SMEGJBVQLJJKKX-HOTMZDKISA-N 0.000 description 1
- 229940081735 acetylcellulose Drugs 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- VREFGVBLTWBCJP-UHFFFAOYSA-N alprazolam Chemical compound C12=CC(Cl)=CC=C2N2C(C)=NN=C2CN=C1C1=CC=CC=C1 VREFGVBLTWBCJP-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011097 chromatography purification Methods 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 108010057788 chymotrypsin B Proteins 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 229940066758 endopeptidases Drugs 0.000 description 1
- 235000019836 ficin Nutrition 0.000 description 1
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011034 membrane dialysis Methods 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- IQFXJRXOTKFGPN-UHFFFAOYSA-N n-ethenyl-n-ethylethanamine Chemical group CCN(CC)C=C IQFXJRXOTKFGPN-UHFFFAOYSA-N 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 229940104658 papain 16 mg Drugs 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 108010066823 proline dipeptidase Proteins 0.000 description 1
- 108010017314 prolyl dipeptidase Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007281 self degradation Effects 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 210000003699 striated muscle Anatomy 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N25/00—Circuitry of solid-state image sensors [SSIS]; Control thereof
- H04N25/70—SSIS architectures; Circuits associated therewith
- H04N25/79—Arrangements of circuitry being divided between different or multiple substrates, chips or circuit boards, e.g. stacked image sensors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
- Y10S435/897—Streptomyces griseus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Signal Processing (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Multimedia (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US00273278A US3806411A (en) | 1972-07-19 | 1972-07-19 | Enzymatic treatment of protein mixtures containing orgotein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2306072A1 DE2306072A1 (de) | 1974-02-07 |
| DE2306072C2 true DE2306072C2 (de) | 1982-06-24 |
Family
ID=23043280
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2306072A Expired DE2306072C2 (de) | 1972-07-19 | 1973-02-08 | Verfahren zur Gewinnung oder Reinigung von Orgotein durch enzymatische Behandlung von Protein-Gemischen |
Country Status (23)
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5811200B2 (ja) * | 1974-05-18 | 1983-03-01 | 鐘淵化学工業株式会社 | テイブンシリヨウ ノ コウボタンパクシツ オ セイゾウスルホウホウ |
| US3962039A (en) * | 1974-08-08 | 1976-06-08 | Center For Laboratory Medicine | Analytical procedure for thyroid hormones |
| FR2548214B1 (fr) * | 1983-06-14 | 1986-09-12 | Edinen Zentar Chim | Procede de traitement de la biomasse en vue de la separation des aminoacides et des lipides |
-
1972
- 1972-07-19 US US00273278A patent/US3806411A/en not_active Expired - Lifetime
- 1972-11-28 IL IL40956A patent/IL40956A/xx unknown
- 1972-11-30 HU HUDA302A patent/HU167256B/hu unknown
- 1972-12-04 AU AU49583/72A patent/AU464345B2/en not_active Expired
- 1972-12-04 ZA ZA728582A patent/ZA728582B/xx unknown
- 1972-12-15 CH CH1833172A patent/CH579915A5/xx not_active IP Right Cessation
- 1972-12-15 JP JP12669572A patent/JPS548756B2/ja not_active Expired
- 1972-12-21 AT AT10935/72A patent/AT320141B/de not_active IP Right Cessation
- 1972-12-28 DD DD168021*A patent/DD101902A5/xx unknown
- 1972-12-28 FI FI723678A patent/FI49170C/fi active
-
1973
- 1973-01-10 FR FR7300783A patent/FR2193084B1/fr not_active Expired
- 1973-01-23 NL NL7300935A patent/NL7300935A/xx not_active Application Discontinuation
- 1973-02-08 DE DE2306072A patent/DE2306072C2/de not_active Expired
- 1973-03-15 PH PH7314431A patent/PH12333A/en unknown
- 1973-04-17 GB GB1856973A patent/GB1423913A/en not_active Expired
- 1973-04-19 IE IE629/73A patent/IE38688B1/xx unknown
- 1973-05-22 SU SU1918551A patent/SU535912A3/ru active
- 1973-05-23 CA CA172,006A patent/CA1007587A/en not_active Expired
- 1973-07-12 PL PL1973164023A patent/PL88837B1/pl unknown
- 1973-07-17 CS CS7300005131A patent/CS182236B2/cs unknown
- 1973-07-17 IT IT51506/73A patent/IT1043862B/it active
- 1973-07-18 DK DK397573AA patent/DK133558B/da unknown
- 1973-07-18 NO NO2936/73A patent/NO137697C/no unknown
Non-Patent Citations (1)
| Title |
|---|
| NICHTS-ERMITTELT |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69432859T2 (de) | Verfahren zur reinigung von kollagenase | |
| EP0409053B1 (de) | Verfahren zur Reinigung von Annexinen | |
| DE2440529C3 (de) | Hyaluronidase-Präparat aus menschlicher Plazenta | |
| DE3032488A1 (de) | Verfahren zur herstellung eines impfstoffs | |
| DE69315388T2 (de) | Aus bakteriellen Proteinen bestehender Extrakt, Verfahren zur dessen Herstellung und diesen Extrakt enthaltende pharmazeutische Zusammensetzung | |
| DE2733565A1 (de) | Verfahren zum isolieren und gewinnen von aus hypophysegeweben stammenden proteinhormonen | |
| DE2306072C2 (de) | Verfahren zur Gewinnung oder Reinigung von Orgotein durch enzymatische Behandlung von Protein-Gemischen | |
| EP0065286B1 (de) | Verfahren zum Reinigen bzw. Anreichern von biologisch aktiven Proteinen und hierzu geeignetes Mittel | |
| DE2715748C3 (de) | Gereinigte aktive Verbindung des Plasminogen-Typs menschlichen Ursprungs und ihre Verwendung | |
| DE1617332C3 (de) | Verfahren zum Isolieren eines wasserlöslichen Protein-Metall-Chelats mit antiphlogistischer Wirkung | |
| DE3034045C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| DE2259405A1 (de) | Verfahren zur isolierung von orgotein aus blutzellen | |
| DE2509482C2 (de) | Verfahren zur Herstellung des Kallikrein-Trypsin-Inhibitors aus Rinderlunge | |
| DE19726626C1 (de) | Verfahren zur Herstellung eines eine Hyaluronidase enthaltenden Präparats | |
| DE1927517A1 (de) | Verfahren zur Gewinnung und Reinigung von L-Asparaginase | |
| DE2259404A1 (de) | Verfahren zur gewinnung von orgotein aus tierischem gewebe | |
| DE1089630B (de) | Verfahren zur Herstellung geruch- und geschmackloser Proteinabbauprodukte aus Blut | |
| DE2828235B1 (de) | Verfahren und Adsorptionsmittel zur Isolierung und Reindarstellung von Enzymen aus einer Roh-Enzym-Loesung | |
| DE2822842C2 (de) | Verfahren zur Herstellung von CoA-SPC Bäckerhefeextrakt sowie Reagenz zum Nachweis von Krebs beim Menschen | |
| DE2632212A1 (de) | Verfahren zur gewinnung von urokinase | |
| DE2333884A1 (de) | Verfahren zur herstellung von gereinigtem albumin und das dabei erhaltene gereinigte albumin | |
| DE3888404T2 (de) | Verfahren zur Unterdrückung des thermischen Abbaus der Urokinase. | |
| DE2635894A1 (de) | Verfahren zur herstellung eines antihaemophiles globulin a enthaltenden konzentrats | |
| DE950594C (de) | Verfahren zur Gewinnung von Heparin | |
| DE3907162C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OD | Request for examination | ||
| D2 | Grant after examination |