DE2305820A1 - Verfahren und vorrichtung zur elektrophorese - Google Patents
Verfahren und vorrichtung zur elektrophoreseInfo
- Publication number
- DE2305820A1 DE2305820A1 DE2305820A DE2305820A DE2305820A1 DE 2305820 A1 DE2305820 A1 DE 2305820A1 DE 2305820 A DE2305820 A DE 2305820A DE 2305820 A DE2305820 A DE 2305820A DE 2305820 A1 DE2305820 A1 DE 2305820A1
- Authority
- DE
- Germany
- Prior art keywords
- medium
- separation
- electrophoresis
- container
- collection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000001962 electrophoresis Methods 0.000 title claims description 65
- 238000000034 method Methods 0.000 title claims description 13
- 238000000926 separation method Methods 0.000 claims description 118
- 239000007787 solid Substances 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 20
- 238000013508 migration Methods 0.000 claims description 17
- 230000005012 migration Effects 0.000 claims description 17
- 230000003287 optical effect Effects 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 4
- 230000002441 reversible effect Effects 0.000 claims description 4
- 239000012056 semi-solid material Substances 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 239000011343 solid material Substances 0.000 claims 1
- 239000002245 particle Substances 0.000 description 17
- 239000007853 buffer solution Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 11
- 239000011521 glass Substances 0.000 description 10
- 239000012530 fluid Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 238000007789 sealing Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229920002307 Dextran Polymers 0.000 description 3
- 230000005684 electric field Effects 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000002826 coolant Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000007863 gel particle Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/4476—Apparatus specially adapted therefor of the density gradient type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Electrostatic Separation (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US00226016A US3847785A (en) | 1972-02-14 | 1972-02-14 | Electrophoresis aparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2305820A1 true DE2305820A1 (de) | 1973-08-30 |
Family
ID=22847211
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2305820A Withdrawn DE2305820A1 (de) | 1972-02-14 | 1973-02-07 | Verfahren und vorrichtung zur elektrophorese |
| DE19737304459U Expired DE7304459U (de) | 1972-02-14 | 1973-02-07 | Vorrichtung zur elektrophorese |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19737304459U Expired DE7304459U (de) | 1972-02-14 | 1973-02-07 | Vorrichtung zur elektrophorese |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US3847785A (enExample) |
| CA (1) | CA969887A (enExample) |
| DE (2) | DE2305820A1 (enExample) |
| FR (1) | FR2172115B3 (enExample) |
| GB (1) | GB1418922A (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4090937A (en) * | 1976-07-28 | 1978-05-23 | Vish Minno-Geoloshki Institute | Electrophoretic technique for varying the concentration of a colloidal solution |
| US4288425A (en) * | 1979-01-31 | 1981-09-08 | Technicon Instruments Corporation | Method and apparatuses for electrophoretic immunoassay |
| US4323439A (en) * | 1979-12-31 | 1982-04-06 | The Regents Of The University Of California | Method and apparatus for dynamic equilibrium electrophoresis |
| US4290855A (en) * | 1979-12-31 | 1981-09-22 | The Regents Of The University Of California | Method of isotope enrichment |
| US4545888A (en) * | 1984-04-06 | 1985-10-08 | Walsh J William | Apparatus for electrophoretic recovery of nucleic acids and other substances |
| US4863647A (en) * | 1984-06-28 | 1989-09-05 | Baylor Jr Charles | Process for preparation of electrophoresis gel material |
| US4790919A (en) * | 1984-06-28 | 1988-12-13 | E. I. Du Pont De Nemours And Company | Process for preparation of electrophoresis gel material |
| US5102518A (en) * | 1989-01-27 | 1992-04-07 | Whitehead Institute For Biomedical Research | Electrophoresis apparatus and method for electroeluting desired molecules for further processing |
| US5246577A (en) * | 1990-05-29 | 1993-09-21 | Millipore Corporation | Apparatus for effecting capillary electrophoresis |
| US5409586A (en) * | 1992-08-26 | 1995-04-25 | Hitachi, Ltd. | Method for analyzing nucleic acid or protein and apparatus therefor |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3384564A (en) * | 1962-11-21 | 1968-05-21 | Mount Sinai Hospital Res Found | Electrophoretic process for simultaneously spearating and concentrating particles |
| US3453200A (en) * | 1966-05-25 | 1969-07-01 | Instrumentation Specialties Co | Apparatus for density gradient electrophoresis |
| US3553097A (en) * | 1967-12-29 | 1971-01-05 | Buchler Instr Inc | Fractional electrophoresis separator |
| US3616454A (en) * | 1969-03-13 | 1971-10-26 | Univ New York | Method of and apparatus for electrophoretic separation in a gel column |
-
1972
- 1972-02-14 US US00226016A patent/US3847785A/en not_active Expired - Lifetime
-
1973
- 1973-01-08 GB GB94273A patent/GB1418922A/en not_active Expired
- 1973-01-11 CA CA161,049A patent/CA969887A/en not_active Expired
- 1973-02-01 FR FR7303491A patent/FR2172115B3/fr not_active Expired
- 1973-02-07 DE DE2305820A patent/DE2305820A1/de not_active Withdrawn
- 1973-02-07 DE DE19737304459U patent/DE7304459U/de not_active Expired
Non-Patent Citations (1)
| Title |
|---|
| NICHTS-ERMITTELT * |
Also Published As
| Publication number | Publication date |
|---|---|
| US3847785A (en) | 1974-11-12 |
| CA969887A (en) | 1975-06-24 |
| FR2172115B3 (enExample) | 1976-01-30 |
| GB1418922A (en) | 1975-12-24 |
| FR2172115A1 (enExample) | 1973-09-28 |
| DE7304459U (de) | 1973-05-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8127 | New person/name/address of the applicant |
Owner name: ISCO, INC., 68504 LINCOLN, NEBRASKA, US |
|
| 8139 | Disposal/non-payment of the annual fee |