DE2258300A1 - Verfahren zur konzentrierung und reinigung von viren - Google Patents
Verfahren zur konzentrierung und reinigung von virenInfo
- Publication number
- DE2258300A1 DE2258300A1 DE19722258300 DE2258300A DE2258300A1 DE 2258300 A1 DE2258300 A1 DE 2258300A1 DE 19722258300 DE19722258300 DE 19722258300 DE 2258300 A DE2258300 A DE 2258300A DE 2258300 A1 DE2258300 A1 DE 2258300A1
- Authority
- DE
- Germany
- Prior art keywords
- virus
- polyethylene glycol
- solution
- suspension
- carbon tetrachloride
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000700605 Viruses Species 0.000 title claims description 30
- 238000000034 method Methods 0.000 title claims description 24
- 238000004140 cleaning Methods 0.000 title claims description 10
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 claims description 20
- 239000002202 Polyethylene glycol Substances 0.000 claims description 18
- 229920001223 polyethylene glycol Polymers 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 12
- 239000000725 suspension Substances 0.000 claims description 9
- 229940093429 polyethylene glycol 6000 Drugs 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 description 11
- 206010022000 influenza Diseases 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000002244 precipitate Substances 0.000 description 8
- 239000012530 fluid Substances 0.000 description 7
- 238000000265 homogenisation Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 5
- 241000287828 Gallus gallus Species 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000011491 glass wool Substances 0.000 description 3
- 238000000464 low-speed centrifugation Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 2
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 2
- 239000011362 coarse particle Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 230000035931 haemagglutination Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 1
- 241000710960 Sindbis virus Species 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 229960000458 allantoin Drugs 0.000 description 1
- 229940037003 alum Drugs 0.000 description 1
- 235000011124 aluminium ammonium sulphate Nutrition 0.000 description 1
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
- LCQXXBOSCBRNNT-UHFFFAOYSA-K ammonium aluminium sulfate Chemical compound [NH4+].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O LCQXXBOSCBRNNT-UHFFFAOYSA-K 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000001085 differential centrifugation Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229960003971 influenza vaccine Drugs 0.000 description 1
- 238000001738 isopycnic centrifugation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16151—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH1762171 | 1971-12-03 | ||
| CH1762071 | 1971-12-03 | ||
| CH1762271 | 1971-12-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2258300A1 true DE2258300A1 (de) | 1973-06-14 |
Family
ID=27177637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19722258300 Pending DE2258300A1 (de) | 1971-12-03 | 1972-11-29 | Verfahren zur konzentrierung und reinigung von viren |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS4862988A (enrdf_load_stackoverflow) |
| BE (1) | BE792217A (enrdf_load_stackoverflow) |
| DE (1) | DE2258300A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2162139A1 (enrdf_load_stackoverflow) |
| NL (1) | NL7216091A (enrdf_load_stackoverflow) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ225583A (en) * | 1987-08-06 | 1991-07-26 | Merck & Co Inc | Process for purifying hepatitis a virions (hav) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1226743A (enrdf_load_stackoverflow) * | 1967-04-19 | 1971-03-31 | ||
| DE2005117A1 (de) * | 1970-02-05 | 1971-08-19 | Farbenfabriken Bayer Aktiengesell schaft, 5090 Leverkusen | Verfahren zur Anreicherung und Reim gung von Losungen des Newcastle Disease Virus |
-
1972
- 1972-11-28 NL NL7216091A patent/NL7216091A/xx unknown
- 1972-11-29 DE DE19722258300 patent/DE2258300A1/de active Pending
- 1972-12-01 FR FR7242716A patent/FR2162139A1/fr active Granted
- 1972-12-01 BE BE792217D patent/BE792217A/xx unknown
- 1972-12-01 JP JP11992972A patent/JPS4862988A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| FR2162139A1 (en) | 1973-07-13 |
| BE792217A (fr) | 1973-06-01 |
| NL7216091A (enrdf_load_stackoverflow) | 1973-06-05 |
| FR2162139B1 (enrdf_load_stackoverflow) | 1976-08-20 |
| JPS4862988A (enrdf_load_stackoverflow) | 1973-09-01 |
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