DE2154881A1 - Verfahren zum Transparentmachen einer Elektrophoresemembran - Google Patents
Verfahren zum Transparentmachen einer ElektrophoresemembranInfo
- Publication number
- DE2154881A1 DE2154881A1 DE19712154881 DE2154881A DE2154881A1 DE 2154881 A1 DE2154881 A1 DE 2154881A1 DE 19712154881 DE19712154881 DE 19712154881 DE 2154881 A DE2154881 A DE 2154881A DE 2154881 A1 DE2154881 A1 DE 2154881A1
- Authority
- DE
- Germany
- Prior art keywords
- membrane
- transparent
- alcohol
- electrophoresis
- ether
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012528 membrane Substances 0.000 title claims description 70
- 238000001962 electrophoresis Methods 0.000 title claims description 15
- 238000000034 method Methods 0.000 title claims description 14
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 235000019441 ethanol Nutrition 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 5
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 claims description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- ONNDGYXYNGPSJT-UHFFFAOYSA-N 2,2-diethyl-1,4-dioxane Chemical compound CCC1(CC)COCCO1 ONNDGYXYNGPSJT-UHFFFAOYSA-N 0.000 claims description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 2
- XOBKSJJDNFUZPF-UHFFFAOYSA-N Methoxyethane Chemical compound CCOC XOBKSJJDNFUZPF-UHFFFAOYSA-N 0.000 claims description 2
- 239000007788 liquid Substances 0.000 description 13
- NNBZCPXTIHJBJL-UHFFFAOYSA-N decalin Chemical compound C1CCCC2CCCCC21 NNBZCPXTIHJBJL-UHFFFAOYSA-N 0.000 description 10
- 239000011521 glass Substances 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 6
- 239000011148 porous material Substances 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
- 229940057995 liquid paraffin Drugs 0.000 description 5
- PXXNTAGJWPJAGM-UHFFFAOYSA-N vertaline Natural products C1C2C=3C=C(OC)C(OC)=CC=3OC(C=C3)=CC=C3CCC(=O)OC1CC1N2CCCC1 PXXNTAGJWPJAGM-UHFFFAOYSA-N 0.000 description 5
- URAYPUMNDPQOKB-UHFFFAOYSA-N triacetin Chemical compound CC(=O)OCC(OC(C)=O)COC(C)=O URAYPUMNDPQOKB-UHFFFAOYSA-N 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000004042 decolorization Methods 0.000 description 3
- 238000001739 density measurement Methods 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 229920001747 Cellulose diacetate Polymers 0.000 description 2
- 229920002284 Cellulose triacetate Polymers 0.000 description 2
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 230000001680 brushing effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000002170 ethers Chemical class 0.000 description 2
- 235000013773 glyceryl triacetate Nutrition 0.000 description 2
- 239000001087 glyceryl triacetate Substances 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 229960002622 triacetin Drugs 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 125000005233 alkylalcohol group Chemical group 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000012461 cellulose resin Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- -1 ζ. B Polymers 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatments Of Macromolecular Shaped Articles (AREA)
- Peptides Or Proteins (AREA)
- Manufacture Of Macromolecular Shaped Articles (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP45096404A JPS5024000B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1970-11-04 | 1970-11-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2154881A1 true DE2154881A1 (de) | 1972-05-10 |
Family
ID=14164008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19712154881 Pending DE2154881A1 (de) | 1970-11-04 | 1971-11-04 | Verfahren zum Transparentmachen einer Elektrophoresemembran |
Country Status (5)
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4084541A (en) * | 1975-04-22 | 1978-04-18 | Olympus Optical Co., Ltd. | Dyeing and decolorization apparatus for use in a blood serum analyzer of an electrophoretic type |
| US4128470A (en) * | 1975-08-29 | 1978-12-05 | Fuji Photo Film Co., Ltd. | Supports for electrophoresis and process for the production of the same |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3230212A (en) * | 1960-04-27 | 1966-01-18 | Frederick A H Rice | Cross-linked cellulosic polymer |
| US3373056A (en) * | 1964-08-28 | 1968-03-12 | Aerojet General Co | Modifying cellulose ester osmotic membranes |
| US3432424A (en) * | 1965-08-23 | 1969-03-11 | Beckman Instruments Inc | Immunoelectrophoresis antiserum strips |
| US3594263A (en) * | 1967-03-27 | 1971-07-20 | Millipore Corp | Laminated sheet for electrophoresis |
| FR1547418A (fr) * | 1967-05-22 | 1968-11-29 | Rhodiaceta | Triacétate de cellulose de caractéristiques nouvelles et procédé pour l'obtenir |
| US3497437A (en) * | 1967-06-21 | 1970-02-24 | Baxter Laboratories Inc | Method of electrophoresis |
| US3553067A (en) * | 1968-03-25 | 1971-01-05 | Millipore Corp | Laminated sheet for evaluating diffusive reactions |
-
1970
- 1970-11-04 JP JP45096404A patent/JPS5024000B1/ja active Pending
-
1971
- 1971-11-02 CA CA127,417A patent/CA966621A/en not_active Expired
- 1971-11-02 GB GB5096971A patent/GB1362873A/en not_active Expired
- 1971-11-03 US US00195420A patent/US3857778A/en not_active Expired - Lifetime
- 1971-11-04 DE DE19712154881 patent/DE2154881A1/de active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US3857778A (en) | 1974-12-31 |
| CA966621A (en) | 1975-04-29 |
| GB1362873A (en) | 1974-08-07 |
| JPS5024000B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1975-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE1256920B (de) | Testindikator zum Nachweis von Ketonverbindungen in Koerperfluessigkeiten und Verfahren zu seiner Herstellung | |
| DE2162122A1 (de) | Indikator zum nachweis von metallionen | |
| DE2130559C3 (de) | Diagnostisches Mittel zum Nach weis von Urobihnogen | |
| DE2826363A1 (de) | Deckfolie zur verwendung in mikroskopischen faerbeverfahren und verfahren zu ihrer herstellung | |
| DE2240471A1 (de) | Diagnostisches mittel zum nachweis von bilirubin | |
| DE2605221C2 (de) | Verfahren zur Herstellung eines diagnostischen Mittels zum Nachweis von Ketonen | |
| DE2154881A1 (de) | Verfahren zum Transparentmachen einer Elektrophoresemembran | |
| DE2921023C2 (de) | Zusammensetzung, Testmittel und Verfahren zum Bestimmen von Urobilinogen in einer Probe unter Verwendung der Zusammensetzung, sowie Verfahren zum Herstellen des Testmittels | |
| DE3540104A1 (de) | Diagnostizierstreifen | |
| DE1172117B (de) | Verfahren zur Herstellung eines lichtbestaendigen photographischen Farbbildes nach dem Farbdiffusionsuebertragungsverfahren unter Verwendung von Farbstoffentwicklersubstanzen | |
| DE2853300C3 (de) | Schnelldiagnostica zur diagnostischen Erfassung von okkultem Blut | |
| DE2834371C2 (de) | Wärmeentwickelbares Diazotypiematerial | |
| EP0041683B1 (de) | Prüfmittel zum Nachweis kuppelnder Verbindungen sowie ein Verfahren zu seiner Herstellung | |
| DE2139563A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| DE1598739C3 (de) | Mittel zur Bestimmung der Chohnesteraseaktivitat im Serum | |
| DE2117246A1 (de) | Verfahren zur Herstellung einer Prüfmittel-Membran | |
| EP0564925A2 (de) | Polarisationsfolie | |
| EP0276442B1 (de) | Eindeckmittel für Mikroskopie-Präparate | |
| DE571692C (de) | Verfahren zum UEbersensibilisieren von photographischen Filmen | |
| DE1911282A1 (de) | Verfahren zum verbesserten Nachweis und zur verbesserten Bestimmung ionisierender Korpuskularstrahlung | |
| DE2158125C3 (de) | Verfahren zur Herstellung eines diagnostischen Mittels zum Nachweis von Ketonkörpem in Körperflüssigkeiten | |
| DE2038207C3 (de) | Verfahren zum Ausbleichen von Sensibillsierungsfarbstoffen in einer photoleitfähkjen Schicht | |
| DE2429647A1 (de) | Verfahren zum faerben von gewebe | |
| DE1904764A1 (de) | Positiv arbeitende lichtempfindliche Flachdruckplatten und Verfahren zu ihrer Herstellung | |
| DE564229C (de) | Celluloseesterueberzug fuer Puppen galvanischer Elemente |